Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,164,571	0	A	C	27.0%	126.3 / 17.4	74	K159N (AAA→AAC)	nagZ	beta‑N‑acetylhexosaminidase
Reads supporting (aligned to +/- strand):  ref base A (39/15);  new base C (0/20);  total (39/35)
Fisher's exact test for biased strand distribution p-value = 5.51e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GCTATGGATATTGATATCAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAAT‑TGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAGATATGTCGGTCTTCAGTTCCTTAATCCGCGAAAATAAACTCGACGCCA  >  NC_000913/1164422‑1164719                                                                                                                                                       |                                                                                                                                                     gCTATGGATATTGATATCAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCACT‑TGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGaaa                                                                                                                                                      >  2:1018209/1‑150 (MQ=255) gCTATGGATATTGATATCAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAAT‑TGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGaaa                                                                                                                                                      <  2:1267161/150‑1 (MQ=255)  cTATGGATATTGATATCAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAAT‑TGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGaaaa                                                                                                                                                     >  1:1544187/1‑150 (MQ=255)      ggATATTGATATCAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAAT‑TGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGAc                                                                                                                                                 >  1:2609887/1‑150 (MQ=255)      ggATATTGATATCAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAAT‑TGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGAc                                                                                                                                                 >  1:2609826/1‑150 (MQ=255)        atatTGATATCAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAAT‑TGCCAGCCGGTTTATTGATGGTCTGCATGAAGCCGGAATGAAAACGACCg                                                                                                                                               >  2:2311660/1‑150 (MQ=255)        atatTGATATCAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAAT‑TGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCg                                                                                                                                               >  2:2308575/1‑150 (MQ=255)           ttGATATCAGCTTTGCGCGTGTGGTGGATGTGGGGCATATCAGCGCGGCGATTGGCGAGCGTTATTATCATGCCGATCCACAAAAAGCCGTGGCATT‑TGCCAGCGGGTTTTTTGTTGTTATGCATTAAGCCGCAATCAACACGACCGGGa                                                                                                                                            <  2:1973534/150‑1 (MQ=255)                atCAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAAT‑TGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAacac                                                                                                                                       >  1:197612/1‑150 (MQ=255)                atCAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAAT‑TGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAacac                                                                                                                                       >  1:197982/1‑150 (MQ=255)                 tCAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAAT‑TGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACt                                                                                                                                      >  2:2168755/1‑150 (MQ=255)                 tCAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAAT‑TGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACt                                                                                                                                      >  2:2168991/1‑150 (MQ=255)                  cAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAAT‑TGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACtt                                                                                                                                     >  2:309127/1‑150 (MQ=255)                    gCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGAAAT‑TGCCAGCCGGTTTATTTTTGGTATGCATGACGCCGGAATTAAAACGACCGGGAAACACTTcc                                                                                                                                   <  2:2173414/150‑1 (MQ=255)                      tttGCGCGTGTGCTGTTTGTCGGGCTTACCCGCGGGGCTATGGGCCAGCGTACTTTTCTTGCCGAACCAAAAAACGCCCTGCCAAT‑TCCCAGCCGGTTTTTTGCTGGTATGCATGAAGCGGCATTGAACACGACCGGGAAACACTTCCCa                                                                                                                                 <  2:482002/150‑1 (MQ=255)                      tttGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTAACATGCCGAACCACAAAAAGCCCTGGAAAT‑TGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCa                                                                                                                                 <  2:2872564/150‑1 (MQ=255)                      ttggcgcCTGTGCGGGATGTCGGCCAAAACCCCCCGGCGATTGGCCAGCGTACTTACCAGCCCCACCCACAAAAACCCCTGGCATT‑GGCCAGCCGTTTTATTTATGGTATGCATGAAGCCGCAATGAAAACGACCGGGAAACACTTCCCa                                                                                                                                 <  2:1523608/147‑1 (MQ=255)                         gcgcCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAAT‑TGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGa                                                                                                                              >  1:1395345/1‑150 (MQ=255)                               gtgCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAAT‑TGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGt                                                                                                                        >  2:363580/1‑150 (MQ=255)                                tgCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAAT‑TGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTg                                                                                                                       >  2:215030/1‑150 (MQ=255)                                  ctgtttGTCGGCAATAACCGCGGGGGGTTGGGCGAGCTTTTTTAACATGCCGATCAACAAAACGCCGTGGAATT‑TGCCAGCCGTTTTTTTTATGTTATGCTTGACGCCGGAATCAACACGACCGGGAAACACTTCCCAGGACACGGTGCa                                                                                                                     <  2:407995/145‑1 (MQ=255)                                       tGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAAT‑TGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAAc                                                                                                                >  1:1278544/1‑150 (MQ=255)                                       tGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAAT‑TGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAAc                                                                                                                >  1:1014314/1‑150 (MQ=255)                                          cGGGCATTTCAGCGGGGCGTTGGGCGAGCTTTCTTATCTGGCCTAACCAAAAAACCCCCTGGAATT‑GGCCCGCGGTTTTATTGTGGGTTTGAATTAAGCCGCAATCAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGc                                                                                                             <  1:2399885/150‑1 (MQ=255)                                           ggtaatatCAGCGCGGGGTTTGGCTTGGGTTATTATCATGCCGAACAACAAAACGCCCTGGAATT‑TGCCAGCCGGTTTTTTGTTGGTTTGCATGAAGCCGCACTCAACACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCa                                                                                                            <  1:2626105/146‑1 (MQ=255)                                           gggCATACCAGCGCGGCGATTGGCGAGCTTTATTATCATCCCGAACCACAAAAAGCCCTGGCAAT‑TGCCAGCCGGTTTTTTGATGGTATGCTTGAAGCCGGAATGAACACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCa                                                                                                            <  2:2171048/150‑1 (MQ=255)                                             gCATATCCGCGCGGCGTTTGGCGAGCGTTCTTATCATGCCTACCCACAAAAAGCCCTGGAAAT‑TGCCAGCGGGTTTTTTTATGTTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGa                                                                                                          <  2:1725450/150‑1 (MQ=255)                                             gCATATCAGCGCGGCTTTTGGCGCGCGTTCTTATCAGGCCCATCCACAAAAAGCCCTGGAATT‑TGCCAGCCGGTTTTTTGTTGGTCTGCATGAAGCCGGAATGAACACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGa                                                                                                          <  2:1725468/150‑1 (MQ=255)                                                 atCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAAT‑TGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTca                                                                                                      >  2:1599747/1‑150 (MQ=255)                                                 aacAGCGCGGCTCTTGGCGCGGGTTCTTCCCAGCCCCACCCACAAAACCCCCT‑GCAATGTGCCCGCCGGTTTTTGTTTGGTTGGCATGAAGCCGTAATCAACACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTca                                                                                                      <  1:2329218/148‑1 (MQ=255)                                                    aGCGCGGCGTTTGGCGAGCTTTCTTATCATGCCGAACCCCAAAAACCCCTGGAAAT‑TGCCAGCGGTTTTATTGTTGGTATGCATGACGCCGTAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTcacac                                                                                                   <  2:940851/150‑1 (MQ=255)                                                        gggcttttGGCGTGGTTTTTTATCATGCCTATCAAAAAAAAGCCCTGTAATT‑TCCCAGCGTTTTTTTTTTTGGTTTGATTCACGCCGTAATCAACACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAg                                                                                               <  1:866744/144‑1 (MQ=255)                                                           ggttgggCTAGGGTTCTTATCAGGCCGACCCACAAAACGCCCTGGAATT‑TGCCAGCGGGTTTATTGTTGGTTGGCATGACGCCGTAATTAACACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGaaa                                                                                            <  1:2603787/145‑1 (MQ=255)                                                                 gctaGCTTTATTATCTTGCCGATCCACAAAAAGCCCTGGAATT‑TGCCAGCGGTTTTATTGTTGTTATGAATGAAGCGGTAATTAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGt                                                                                      <  2:998680/147‑1 (MQ=255)                                                                     ggtttCTTATCATGCCTACCCAAAAACCCCCGGGGCATT‑GGCCCGCGGGTTTATTGTTGTTATGATTGACGCCGCAATCAACACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGa                                                                                  <  1:490731/147‑1 (MQ=255)                                                                       gTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAAT‑TGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATc                                                                                >  1:31748/1‑150 (MQ=255)                                                                       gTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAAT‑TGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATc                                                                                >  1:2186732/1‑150 (MQ=255)                                                                                    ctaaccAAAAAAAGCCCTGGCATT‑GGCCAGCCGGTTTTTTGTTGTTATGCATGAAGCCGTAATTAACACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGc                                                                   <  2:1395345/146‑1 (MQ=255)                                                                                    cGATCCACAAAAAGCCCTGGCAAT‑TGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGc                                                                   >  2:821761/1‑150 (MQ=255)                                                                                     gATCCACAAAAAGCCCTGGCAAT‑TGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCg                                                                  >  1:2702814/1‑150 (MQ=255)                                                                                       tcaacaAAAAGCCCTGGCAAT‑TGCCAGCCGGTTTATTGATGGTATGCATCAAGCCGCAATCAACACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGga                                                                <  2:1444846/147‑1 (MQ=255)                                                                                         cacaAAAAGCCCTGGCAAT‑TGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGgaga                                                              >  1:2318141/1‑150 (MQ=255)                                                                                          acaAAAAGCCCTGGCAAT‑TGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGAt                                                             >  1:1978721/1‑150 (MQ=255)                                                                                          acaAAAAGCCCTGGCAAT‑TGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGAt                                                             >  2:1584046/1‑150 (MQ=255)                                                                                           caAAAAGCCCTGGCAAT‑TGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGAtt                                                            >  1:1450495/1‑150 (MQ=255)                                                                                            aaaaaGCCCTGGCAAT‑TGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTc                                                           >  1:2432902/1‑150 (MQ=255)                                                                                                aGCCCTGGCAAT‑TGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCgcgc                                                       >  1:154800/1‑150 (MQ=255)                                                                                                 gCCCTGGCAAT‑TGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCt                                                      >  2:780874/1‑150 (MQ=255)                                                                                                  cccTGGCAAT‑TGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTa                                                     >  1:1827606/1‑150 (MQ=255)                                                                                                   ccTGGCAAT‑TGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTaa                                                    >  2:2728003/1‑150 (MQ=255)                                                                                                   ccTGGCAAT‑TGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTaa                                                    >  1:1639664/1‑150 (MQ=255)                                                                                                   ccTGGAATT‑TGCCAGCGGTTTTTTTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTaa                                                    <  2:2573362/150‑1 (MQ=255)                                                                                                    cTGGCAAT‑TGCCCGCCGGTTTATTGTTGTTAGGCATTAAGCCGGAATGAACACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTaaa                                                   <  2:1827606/150‑1 (MQ=255)                                                                                                     tGGCAAT‑TGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAg                                                  >  1:1645924/1‑150 (MQ=255)                                                                                                       gaatt‑tGCCAGCGGGTTTTTTTTTGGTAGGATTGACGCGGTAATTAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAGat                                                <  2:31748/146‑1 (MQ=255)                                                                                                           t‑tGCCAGCCGTTTTTTTTTTGGTATGCATGAAGCCGGAATCAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAGATATGt                                            <  2:2289410/150‑1 (MQ=255)                                                                                                           t‑tGCCAGCCGTTTTTTTGTTTTTATGCTTTAAGCCTTATTTAACACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAGATATGt                                            <  1:1018209/150‑1 (MQ=255)                                                                                                              gCCAGCCGTTTTATTGATTGTAGGCATGACGCCGGAATTAACACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAGATATGTCg                                          <  2:884174/150‑1 (MQ=255)                                                                                                              gCCAGCCGGTTTATTGATGGTATGCATGAAGCCGTAATTAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAGATATGTCg                                          <  2:884566/150‑1 (MQ=255)                                                                                                               ccAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAGATATGTCgg                                         >  1:2201228/1‑150 (MQ=255)                                                                                                                   cggtttttttGTTGGTATGAATGAAGCCGAAATTAACACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAGATATGTCGGTCtt                                     <  2:1014314/146‑1 (MQ=255)                                                                                                                   cggttttATTGTTGTTATGCATGACGCCGTAATGAACACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAGATATGTCGGTCtt                                     <  2:1450495/146‑1 (MQ=255)                                                                                                                   cggttttATTGATGTTATGCATGAAGCCGTAATCAACACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAGATATGTCGGTCtt                                     <  2:1278544/146‑1 (MQ=255)                                                                                                                       ttttttGATGGTATGCTTGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAGATATGTCGGTCTTCAGt                                 <  2:2639153/150‑1 (MQ=255)                                                                                                                          aTTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAGATATGTCGGTCTTCAGTTcc                              >  2:1211097/1‑150 (MQ=255)                                                                                                                            tGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAGATATGTCGGTCTTCAGTTCCtt                            >  2:968670/1‑150 (MQ=255)                                                                                                                              ttGGTATGCATGAAGCCGGAATGAACACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAGATATGTCGGTCTTCAGTTCCTTaa                          <  1:2810946/149‑1 (MQ=255)                                                                                                                               tGGTATGCATGAAGCCGTAATGAACACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAGATATGTCGGTCTTCAGTTCCTTAAt                         <  2:2609887/150‑1 (MQ=255)                                                                                                                               tGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAGATATGTCGGTCTTCAGTTCCTTAAt                         <  2:2609826/150‑1 (MQ=255)                                                                                                                                   atgatttaaGCCGTAATTAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAGATATGTCGGTCTTCAGTTCCTTAATCcgc                     <  2:1544187/143‑1 (MQ=255)                                                                                                                                   aTGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAGATATGTCGGTCTTCAGTTCCTTAATCcgc                     <  2:2201228/150‑1 (MQ=255)                                                                                                                                      cATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAGATATGTCGGTCTTCAGTTCCTTAATCCGCGaa                  >  1:1874201/1‑150 (MQ=255)                                                                                                                                       aTGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAGATATGTCGGTCTTCAGTTCCTTAATCCGCGaaa                 >  1:289807/1‑150 (MQ=255)                                                                                                                                         gAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAGATATGTCGGTCTTCAGTTCCTTAATCCGCGAAAAt               >  1:355215/1‑150 (MQ=255)                                                                                                                                            gCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAGATATGTCGGTCTTCAGTTCCTTAATCCGCGAAAATaaa            >  1:2051441/1‑150 (MQ=255)                                                                                                                                                gAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAGATATGTCGGTCTTCAGTTCCTTAATCCGCGAAAATAAACTCg        >  1:2678418/1‑150 (MQ=255)                                                                                                                                                gAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAGATATGTCGGTCTTCAGTTCCTTAATCCGCGAAAATAAACTCg        >  2:726855/1‑150 (MQ=255)                                                                                                                                                      aaaCGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAGATATGTCGGTCTTCAGTTCCTTAATCCGCGAAAATAAACTCGACGCCa  >  2:2207408/1‑150 (MQ=255)                                                                                                                                                      aaaCGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAGATATGTCGGTCTTCAGTTCCTTAATCCGCGAAAATAAACTCGACGCCa  >  2:1282609/1‑150 (MQ=255)                                                                                                                                                       |                                                                                                                                                     GCTATGGATATTGATATCAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAAT‑TGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAGATATGTCGGTCTTCAGTTCCTTAATCCGCGAAAATAAACTCGACGCCA  >  NC_000913/1164422‑1164719

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑