BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,128,359	C→T	A13T (GCT→ACT)	metJ ←	DNA‑binding transcriptional repressor MetJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,128,359	0	C	T	100.0%	1046.3 / NA	278	A13T (GCT→ACT)	metJ	DNA‑binding transcriptional repressor MetJ
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (135/143); total (135/143)

Only 100 of 279 total aligned reads displayed.

ACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGCGTTACCGTGACATCGTG  >  NC_000913/4128212‑4128505

aCGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAgtgt                                                                                                                                                  >  2:2305347/1‑150 (MQ=255)

        cacaGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTg                                                                                                                                          >  1:230010/1‑150 (MQ=255)

         acaGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGa                                                                                                                                         >  2:817403/1‑150 (MQ=255)

           agcagcTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGata                                                                                                                                       >  1:1030408/1‑150 (MQ=255)

               gcTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATAtt                                                                                                                                   <  2:2019605/150‑1 (MQ=255)

               gcTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATAtt                                                                                                                                   <  2:1727144/150‑1 (MQ=255)

                cTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTc                                                                                                                                  <  2:1196625/150‑1 (MQ=255)

                cTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGCGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTc                                                                                                                                  >  2:875785/1‑150 (MQ=255)

                 tCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTcg                                                                                                                                 >  2:101756/1‑150 (MQ=255)

                  cGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTcgc                                                                                                                                <  1:214864/150‑1 (MQ=255)

                   gCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGatat                                                                                                                                      <  1:2424002/143‑1 (MQ=255)

                   gCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTcgcc                                                                                                                               >  1:2915651/1‑150 (MQ=255)

                     tGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTcgccgc                                                                                                                             >  1:1510406/1‑150 (MQ=255)

                          gTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATCTATTCGCCGCTCCAt                                                                                                                        >  2:1074802/1‑150 (MQ=255)

                             gCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCa                                                                                                                     <  2:49982/150‑1 (MQ=255)

                                tGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGcc                                                                                                                  <  1:1278669/150‑1 (MQ=255)

                                  aCGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCAt                                                                                                                <  1:1005577/150‑1 (MQ=255)

                                  aCGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCAt                                                                                                                >  2:684168/1‑150 (MQ=255)

                                  aCGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCAt                                                                                                                >  1:2592106/1‑150 (MQ=255)

                                    gCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATga                                                                                                              >  2:255883/1‑150 (MQ=255)

                                    gCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATga                                                                                                              <  2:1118422/150‑1 (MQ=255)

                                     cAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATgag                                                                                                             <  1:2604406/150‑1 (MQ=255)

                                       ggTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGAt                                                                                                           <  2:724991/150‑1 (MQ=255)

                                         ttgttCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATAc                                                                                                         <  1:407954/150‑1 (MQ=255)

                                           gttCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACtt                                                                                                       >  2:1051504/1‑150 (MQ=255)

                                            ttCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTa                                                                                                      <  2:1426263/150‑1 (MQ=255)

                                             tCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTaa                                                                                                     <  1:2303033/150‑1 (MQ=255)

                                               aCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATc                                                                                                   >  2:1767844/1‑150 (MQ=255)

                                               aCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATc                                                                                                   <  1:580798/150‑1 (MQ=255)

                                               aCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCATTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATc                                                                                                   <  1:507051/150‑1 (MQ=255)

                                                 cTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCct                                                                                                 >  1:1567556/1‑150 (MQ=255)

                                                   gacgacGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCctct                                                                                               <  2:398053/150‑1 (MQ=255)

                                                     cgacgCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTc                                                                                             <  1:459301/150‑1 (MQ=255)

                                                       acgCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGt                                                                                           <  1:2224421/150‑1 (MQ=255)

                                                          cgtgcgtTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCaa                                                                                        <  1:1898375/150‑1 (MQ=255)

                                                                ttCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAAtt                                                                                  <  1:2691961/150‑1 (MQ=255)

                                                                ttCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAAtt                                                                                  >  1:1801825/1‑150 (MQ=255)

                                                                 tCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTg                                                                                 >  1:610998/1‑150 (MQ=255)

                                                                  cATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTga                                                                                <  2:1908487/150‑1 (MQ=255)

                                                                  cATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTga                                                                                <  2:615500/150‑1 (MQ=255)

                                                                  cATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTga                                                                                <  1:2409885/150‑1 (MQ=255)

                                                                   ataggTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTAAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTgat                                                                               >  1:2504365/4‑149 (MQ=255)

                                                                     cGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGagac                                                                             <  1:1963187/150‑1 (MQ=255)

                                                                       gTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGagacca                                                                           >  2:678316/1‑150 (MQ=255)

                                                                        tGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGagaccag                                                                          >  1:1219758/1‑150 (MQ=255)

                                                                          aGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGagaccagac                                                                        >  2:525874/1‑150 (MQ=255)

                                                                          aGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGagaccagac                                                                        <  1:1178402/150‑1 (MQ=255)

                                                                               ttttAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACcacac                                                                   >  2:421762/1‑150 (MQ=255)

                                                                               ttttAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACcacac                                                                   >  1:2765188/1‑150 (MQ=255)

                                                                                   aaCACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGtt                                                               <  2:1239731/150‑1 (MQ=255)

                                                                                    acacCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTg                                                              >  2:143653/1‑150 (MQ=255)

                                                                                      acCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGAt                                                            >  2:2846573/1‑150 (MQ=255)

                                                                                      acCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGAt                                                            <  2:2347150/150‑1 (MQ=255)

                                                                                        cTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGAtgt                                                          <  2:2421390/150‑1 (MQ=255)

                                                                                        cTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGAtgt                                                          >  2:1826237/1‑150 (MQ=255)

                                                                                          tAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTgg                                                        <  1:765375/150‑1 (MQ=255)

                                                                                            agagGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGt                                                      >  2:1377436/1‑150 (MQ=255)

                                                                                             gagGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTa                                                     >  1:985161/1‑150 (MQ=255)

                                                                                                 aaTGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGa                                                 <  2:1316383/150‑1 (MQ=255)

                                                                                                 aaTGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGa                                                 <  1:2466022/150‑1 (MQ=255)

                                                                                                  aTGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGAc                                                >  2:1980727/1‑150 (MQ=255)

                                                                                                   tGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGAcc                                               <  1:1755208/150‑1 (MQ=255)

                                                                                                   tGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGAcc                                               >  2:2342404/1‑150 (MQ=255)

                                                                                                     gAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGt                                             >  1:2089395/1‑150 (MQ=255)

                                                                                                      aaaCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTa                                            >  1:2650077/1‑150 (MQ=255)

                                                                                                        aCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTaaa                                          >  1:1072458/1‑150 (MQ=255)

                                                                                                          cGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAAcc                                        >  1:664527/1‑150 (MQ=255)

                                                                                                          cGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAAcc                                        >  2:2968271/1‑150 (MQ=255)

                                                                                                            tAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCg                                      >  1:49742/1‑150 (MQ=255)

                                                                                                             aaTCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGc                                     <  1:1629545/150‑1 (MQ=255)

                                                                                                              aTCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCa                                    <  2:1537832/150‑1 (MQ=255)

                                                                                                                 tttttGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAg                                 >  2:1614406/1‑150 (MQ=255)

                                                                                                                 tttttGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAg                                 >  1:876143/1‑150 (MQ=255)

                                                                                                                  ttttGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGt                                >  2:1586556/1‑150 (MQ=255)

                                                                                                                   tttGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGtt                               >  1:2957703/1‑150 (MQ=255)

                                                                                                                      gACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTAc                            <  2:2611747/150‑1 (MQ=255)

                                                                                                                       aCTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTAcc                           <  1:198321/150‑1 (MQ=255)

                                                                                                                       aCTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTAcc                           <  2:2743362/150‑1 (MQ=255)

                                                                                                                       aCTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTAcc                           <  2:2122251/150‑1 (MQ=255)

                                                                                                                       aCTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTAcc                           <  2:1709003/150‑1 (MQ=255)

                                                                                                                        cTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCg                          <  2:290324/150‑1 (MQ=255)

                                                                                                                         ttGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGt                         >  1:2536287/1‑150 (MQ=255)

                                                                                                                            ttCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACa                      <  1:2042286/150‑1 (MQ=255)

                                                                                                                              cACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAgg                    >  2:2668847/1‑150 (MQ=255)

                                                                                                                              cACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAgg                    >  1:1406122/1‑150 (MQ=255)

                                                                                                                               aCTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGc                   <  1:2189566/150‑1 (MQ=255)

                                                                                                                                 tcttctTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGCGt                 >  2:2562325/1‑150 (MQ=255)

                                                                                                                                    tcttGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGCGTTAc              >  1:2332669/1‑150 (MQ=255)

                                                                                                                                    tcttGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGCGTTAc              <  1:698413/150‑1 (MQ=255)

                                                                                                                                     cttGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGCGTTAcc             <  2:2185810/150‑1 (MQ=255)

                                                                                                                                      ttGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGCGTTACCg            >  1:2100406/1‑150 (MQ=255)

                                                                                                                                         ccGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGCGTTACCGTGa         >  1:1579096/1‑150 (MQ=255)

                                                                                                                                         ccGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGCGTTACCGTGa         >  2:2777626/1‑150 (MQ=255)

                                                                                                                                         ccGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGCGTTACCGTGa         >  2:2757042/1‑150 (MQ=255)

                                                                                                                                          cGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGCGTTACCGTGAc        <  1:2125262/150‑1 (MQ=255)

                                                                                                                                           gTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGCGTTACCGTGACa       <  1:2747738/150‑1 (MQ=255)

                                                                                                                                            tGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGCGTTACCGTGACAt      <  1:558388/150‑1 (MQ=255)

                                                                                                                                             gCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGCGTTACCGTGACATc     <  1:2846573/150‑1 (MQ=255)

                                                                                                                                               tCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGCGTTACCGTGACATCgt   <  2:2433626/150‑1 (MQ=255)

                                                                                                                                               tCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGCGTTACCGTGACATCgt   <  2:1064954/150‑1 (MQ=255)

ACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGCGTTACCGTGACATCGTG  >  NC_000913/4128212‑4128505

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`