Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,917,345 G→A G763G (GGG→GGA barA → sensory histidine kinase BarA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,917,3450GA99.1% 390.4 / ‑5.3 105G763G (GGG→GGAbarAsensory histidine kinase BarA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (49/55);  minor base C (1/0);  total (50/55)
Fisher's exact test for biased strand distribution p-value = 4.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 105 total aligned reads displayed.

GATCTTAATGGATATTCAAATGCCGGACATGGATGGCATTCGGGCCTGCGAACTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGGATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGCGTCGTGACGCCCGAAGTCAACGAAATTGTGGTGAACCCGAATGCGACCCTCGACTG  >  NC_000913/2917198‑2917491
                                                                                                                                                   |                                                                                                                                                  
gATCTTAATGGATATTCAAATGCCGGACATGGATGGCATTCGGGCCTGCGAACTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAAt                                                                                                                                                  <  2:1065322/150‑1 (MQ=255)
       aTGGATATTCAAATGCCGGACATGGATGGCATTCGGGCCTGCGAACTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTCACGGCGCATGCAATGGCCGGGCCAATAGTGAAGCTGCTTGGCGCCGGCCTGAGCGAt                                                                                                                                           >  2:2221455/1‑150 (MQ=255)
        tGGATATTCAAATGCCGGACATGGATGGCATTCGGGCCTGCGAACTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGAtt                                                                                                                                          <  2:100431/150‑1 (MQ=255)
           atatTCAAATGCCGGACATGGATGGCATTCGGGCCTGCGAACTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATc                                                                                                                                       >  1:2697115/1‑150 (MQ=255)
           atatTCAAATGCCGGACATGGATGGCATTCGGGCCTGCGAACTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATc                                                                                                                                       <  1:1900709/150‑1 (MQ=255)
              ttCAAATGCCGGACATGGATGGCATTCGGGCCTGCGAACTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTgg                                                                                                                                    >  1:2737334/1‑150 (MQ=255)
              ttCAAATGCCGGACATGGATGGCATTCGGGCCTGCGAACTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTgg                                                                                                                                    <  1:509856/150‑1 (MQ=255)
              ttCAAATGCCGGACATGGATGGCATTCGGGCCTGCGAACTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTgg                                                                                                                                    >  1:2737452/1‑150 (MQ=255)
               tCAAATGCCGGACATGGATGGCATTCGGGCCTGCGAACTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGc                                                                                                                                   <  2:1667535/150‑1 (MQ=255)
               tCAAATGCCGGACATGGATGGCATTCGGGCCTGCGAACTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGc                                                                                                                                   <  1:1134626/150‑1 (MQ=255)
               tCAAATGCCGGACATGGATGGCATTCGGGCCTGCGAACTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGc                                                                                                                                   <  1:1136338/150‑1 (MQ=255)
                 aaaTGCCGGACATGGATGGCATTCGGGCCTGCGAACTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGa                                                                                                                                 >  2:2695379/1‑150 (MQ=255)
                 aaaTGCCGGACATGGATGGCATTCGGGCCTGCGAACTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGa                                                                                                                                 >  2:967786/1‑150 (MQ=255)
                  aaTGCCGGACATGGATGGCATTCGGGCCTGCGAACTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGaa                                                                                                                                >  1:2608290/1‑150 (MQ=255)
                   aTGCCGGACATGGATGGCATTCGGGCCTGCGAACTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGaaa                                                                                                                               <  1:364093/150‑1 (MQ=255)
                    tGCCGGACATGGATGGCATTCGGGCCTGCGAACTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAAc                                                                                                                              >  2:1085508/1‑150 (MQ=255)
                    tGCCGGACATGGATGGCATTCGGGCCTGCGAACTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAAc                                                                                                                              >  2:1085507/1‑150 (MQ=255)
                       cGGACATGGATGGCATTCGGGCCTGCGAACTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGa                                                                                                                           >  2:1432745/1‑150 (MQ=255)
                         gACATGGATGGCATTCGGGCCTGCGAACTCATCCACCAACTCCCGCATCAGCAACACACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGAtt                                                                                                                         <  2:1232458/150‑1 (MQ=255)
                         gACATGGATGGCATTCGGGCCTGCGAACTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGAtt                                                                                                                         <  2:1232438/150‑1 (MQ=255)
                          aCATGGATGGCATTCGGGCCTGCGAACTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTg                                                                                                                        <  1:1482165/150‑1 (MQ=255)
                                atggCATTCGGGCCTGCGAACTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGaagaag                                                                                                                  >  1:190501/1‑150 (MQ=255)
                                 tggCATTCGGGCCTGCGAACTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAagaaga                                                                                                                 <  2:228400/150‑1 (MQ=255)
                                  ggCATTCGGGCCTGCGAACTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAagag                                                                                                                <  1:941382/150‑1 (MQ=255)
                                     aTTCGGGCCTGCGAACTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGa                                                                                                             <  1:2505063/150‑1 (MQ=255)
                                     aTTCGGGCCTGCGAACTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGa                                                                                                             >  2:874103/1‑150 (MQ=255)
                                       tCGGGCCTGCGAACTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGAtt                                                                                                           <  1:1104998/150‑1 (MQ=255)
                                       tCGGGCCTGCGAACTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGAtt                                                                                                           <  1:1413344/150‑1 (MQ=255)
                                         gggCCTGCGAACTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGc                                                                                                         >  2:877093/1‑150 (MQ=255)
                                         gggCCTGCGAACTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGc                                                                                                         >  1:2022017/1‑150 (MQ=255)
                                             cTGCGAACTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATaa                                                                                                     <  2:1013891/150‑1 (MQ=255)
                                              tGCGAACTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAAt                                                                                                    <  2:406836/150‑1 (MQ=255)
                                                 gAACTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATttg                                                                                                 <  1:2249025/150‑1 (MQ=255)
                                                 gAACTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATttg                                                                                                 >  1:2946382/1‑150 (MQ=255)
                                                  aaCTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATttgt                                                                                                <  2:1576673/150‑1 (MQ=255)
                                                  aaCTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATttgt                                                                                                <  1:247917/150‑1 (MQ=255)
                                                     tCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATttgttgt                                                                                             >  2:378835/1‑150 (MQ=255)
                                                     tCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGAAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATtttttgt                                                                                             >  2:378842/1‑150 (MQ=255)
                                                      cATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATttgttgtt                                                                                            <  2:2608290/150‑1 (MQ=255)
                                                       aTCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATttgttgttg                                                                                           >  1:473846/1‑150 (MQ=255)
                                                       aTCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATttgttgttg                                                                                           >  1:473392/1‑150 (MQ=255)
                                                       aTCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATttgttgttg                                                                                           >  2:1168604/1‑150 (MQ=255)
                                                       aTCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATttgttgttg                                                                                           >  1:1003342/1‑150 (MQ=255)
                                                         ccaccaACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTgcg                                                                                         >  2:2208205/1‑150 (MQ=255)
                                                         ccaccaACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTgcg                                                                                         >  2:2198962/1‑150 (MQ=255)
                                                          caccaACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTgcgc                                                                                        <  2:2000192/150‑1 (MQ=255)
                                                           accaACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCt                                                                                       >  1:951681/1‑150 (MQ=255)
                                                           accaACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCt                                                                                       >  1:952340/1‑150 (MQ=255)
                                                           accaACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCt                                                                                       >  1:1556068/1‑150 (MQ=255)
                                                           accaACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCt                                                                                       >  1:1899396/1‑150 (MQ=255)
                                                                   ccGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCt                                                                               >  1:2317111/1‑150 (MQ=255)
                                                                   ccGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCt                                                                               <  2:1753838/150‑1 (MQ=255)
                                                                   ccGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCt                                                                               >  1:2316868/1‑150 (MQ=255)
                                                                     gCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCACTGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTgg                                                                             <  2:1558280/150‑1 (MQ=255)
                                                                      cATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGc                                                                            <  1:288208/150‑1 (MQ=255)
                                                                        tCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAg                                                                          >  2:2817362/1‑150 (MQ=255)
                                                                          aGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCg                                                                        <  2:2697115/150‑1 (MQ=255)
                                                                             aacaaACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTa                                                                     >  2:2620344/1‑150 (MQ=255)
                                                                             aacaaACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTa                                                                     >  1:2342930/1‑150 (MQ=255)
                                                                              acaaACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGACTGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTAt                                                                    >  1:1942699/1‑150 (MQ=255)
                                                                                aaaCGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTAttt                                                                  >  1:748336/1‑150 (MQ=255)
                                                                                aaaCGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTAttt                                                                  >  1:2023465/1‑150 (MQ=255)
                                                                                aaaCGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTAttt                                                                  >  2:1845607/1‑150 (MQ=255)
                                                                                 aaCGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTc                                                                 <  1:2695379/150‑1 (MQ=255)
                                                                                 aaCGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTc                                                                 <  1:967786/150‑1 (MQ=255)
                                                                                 aaCGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTc                                                                 <  1:1432745/150‑1 (MQ=255)
                                                                                    gCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCctc                                                              >  1:1616941/1‑150 (MQ=255)
                                                                                    gCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCctc                                                              <  2:2048814/150‑1 (MQ=255)
                                                                                     ccGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCctct                                                             >  1:803036/1‑150 (MQ=255)
                                                                                     ccGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCctct                                                             >  1:803113/1‑150 (MQ=255)
                                                                                       ggTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTcg                                                           >  1:1053880/1‑150 (MQ=255)
                                                                                        gTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTcgc                                                          >  2:699982/1‑150 (MQ=255)
                                                                                            tCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGcgtcg                                                      <  1:307218/150‑1 (MQ=255)
                                                                                              gcgGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGCGTCGTg                                                    >  1:2962987/1‑150 (MQ=255)
                                                                                                  tAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGCGTCGTGACGc                                                <  1:949357/150‑1 (MQ=255)
                                                                                                  tAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGCGTCGTGACGc                                                <  2:1899396/150‑1 (MQ=255)
                                                                                                    aCGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGCGTCGTGACGccc                                              <  2:2737452/150‑1 (MQ=255)
                                                                                                    aCGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGCGTCGTGACGccc                                              <  2:2737334/150‑1 (MQ=255)
                                                                                                         gcATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGCGTCGTGACGCCCGAAGt                                         <  1:717150/150‑1 (MQ=255)
                                                                                                         gcATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGCGTCGTGACGCCCGAAGt                                         <  1:874103/150‑1 (MQ=255)
                                                                                                          cATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGCGTCGTGACGCCCGAAGTc                                        >  2:1364194/1‑150 (MQ=255)
                                                                                                                   gCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGCGTCGTGACGCCCGAAGTCAACGAAAtt                               <  1:1215257/150‑1 (MQ=255)
                                                                                                                       ggCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGCGTCGTGACGCCCGAAGTCAACGAAATTgtgg                           >  1:1903312/1‑150 (MQ=255)
                                                                                                                        gCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGCGTCGTGACGCCCGAAGTCAACGAAATTgtggt                          >  2:2623755/1‑150 (MQ=255)
                                                                                                                           aaaaGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGCGTCGTGACGCCCGAAGTCAACGAAATTGTGGTGaa                       >  2:1427426/1‑150 (MQ=255)
                                                                                                                            aaaGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGCGTCGTGACGCCCGAAGTCAACGAAATTGTGGTGAAc                      >  2:2407201/1‑150 (MQ=255)
                                                                                                                               gagaAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGCGTCGTGACGCCCGAAGTCAACGAAATTGTGGTGAACCCg                   >  2:2958037/1‑150 (MQ=255)
                                                                                                                               gagaAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGCGTCGTGACGCCCGAAGTCAACGAAATTGTGGTGAACCCg                   >  1:1586950/1‑150 (MQ=255)
                                                                                                                                  aaGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGCGTCGTGACGCCCGAAGTCAACGAAATTGTGGTGAACCCGAAt                <  1:530802/150‑1 (MQ=255)
                                                                                                                                  aaGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGCGTCGTGACGCCCGAAGTCAACGAAATTGTGGTGAACCCGAAt                <  2:1897206/150‑1 (MQ=255)
                                                                                                                                  aaGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGCGTCGTGACGCCCGAAGTCAACGAAATTGTGGTGAACCCGAAt                <  2:1897603/150‑1 (MQ=255)
                                                                                                                                      tgctTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGCGTCGTGACGCCCGAAGTCAACGAAATTGTGGTGAACCCGAATGCGa            <  1:877093/150‑1 (MQ=255)
                                                                                                                                       gctTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGCGTCGTGACGCCCGAAGTCAACGAAATTGTGGTGAACCCGAATGCGAc           <  1:2786506/150‑1 (MQ=255)
                                                                                                                                        ctTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGCGTCGTGACGCCCGAAGTCAACGAAATTGTGGTGAACCCGAATGCGAcc          <  2:1556068/150‑1 (MQ=255)
                                                                                                                                         ttGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGCGTCGTGACGCCCGAAGTCAACGAAATTGTGGTGAACCCGAATGCGAccc         <  2:2868832/150‑1 (MQ=255)
                                                                                                                                          tGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGCGTCGTGACGCCCGAAGTCAACGAAATTGTGGTGAACCCGAATGCGACCCt        <  2:644621/150‑1 (MQ=255)
                                                                                                                                          tGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGCGTCGTGACGCCCGAAGTCAACGAAATTGTGGTGAACCCGAATGCGACCCt        <  2:644630/150‑1 (MQ=255)
                                                                                                                                             cgcAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGCGTCGTGACGCCCGAAGTCAACGAAATTGTGGTGAACCCGAATGCGACCCTCGa     <  1:1364194/150‑1 (MQ=255)
                                                                                                                                               cAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGCGTCGTGACGCCCGAAGTCAACGAAATTGTGGTGAACCCGAATGCGACCCTCGact   <  2:2022017/150‑1 (MQ=255)
                                                                                                                                                aGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGCGTCGTGACGCCCGAAGTCAACGAAATTGTGGTGAACCCGAATGCGACCCTCGactg  <  2:2120795/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GATCTTAATGGATATTCAAATGCCGGACATGGATGGCATTCGGGCCTGCGAACTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGGATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGCGTCGTGACGCCCGAAGTCAACGAAATTGTGGTGAACCCGAATGCGACCCTCGACTG  >  NC_000913/2917198‑2917491

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: