Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913164,5640AT30.4% 175.0 / 14.1 102intergenic (+30/‑166)hrpB/mrcBputative ATP‑dependent RNA helicase HrpB/peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcB
Reads supporting (aligned to +/- strand):  ref base A (33/31);  new base T (0/28);  total (33/69)
Fisher's exact test for biased strand distribution p-value = 2.01e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 113 total aligned reads displayed.

TTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAA  >  NC_000913/164415‑164712
                                                                                                                                                       |                                                                                                                                                    
ttCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGgaga                                                                                                                                                      <  1:2438292/150‑1 (MQ=255)
ttCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGgaga                                                                                                                                                      <  2:2496131/150‑1 (MQ=255)
ttCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGgaga                                                                                                                                                      <  1:1962753/150‑1 (MQ=255)
ttCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGgaga                                                                                                                                                      <  1:398011/150‑1 (MQ=255)
ttCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCACAGGgaga                                                                                                                                                      <  2:1982297/150‑1 (MQ=255)
 tCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGagag                                                                                                                                                     <  2:357972/150‑1 (MQ=255)
 tCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAACGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGagag                                                                                                                                                     <  2:1433502/150‑1 (MQ=255)
  cTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAgg                                                                                                                                                    >  1:2179571/1‑150 (MQ=255)
   tGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAggg                                                                                                                                                   >  1:70993/1‑150 (MQ=255)
   tGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCACAGGGAGAggg                                                                                                                                                   <  1:85434/150‑1 (MQ=255)
    ggAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAggga                                                                                                                                                  <  1:962653/150‑2 (MQ=255)
    ggAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGc                                                                                                                                                  <  2:463342/150‑1 (MQ=255)
    ggAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGc                                                                                                                                                  >  2:2146090/1‑150 (MQ=255)
     gAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGcc                                                                                                                                                 >  2:42459/1‑150 (MQ=255)
     gAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGcc                                                                                                                                                 >  2:169509/1‑150 (MQ=255)
     gAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGcc                                                                                                                                                 >  1:1090423/1‑150 (MQ=255)
      ataGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCg                                                                                                                                                <  1:2610995/148‑1 (MQ=255)
      aaaGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCg                                                                                                                                                <  1:2610948/150‑1 (MQ=255)
       aaGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCgg                                                                                                                                               <  2:1737567/150‑1 (MQ=255)
       aaGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCgg                                                                                                                                               >  2:563238/1‑150 (MQ=255)
        aGGTGCGTACCGTTAGGTGAAAAAAGAGATGAAAGGGCGTTATCCCAATCTTTTCTGGCCGGACGACCCTGCAAATACTGCACCGACGCGACGGACGAAAAAGTTTTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGTGGGCCggg                                                                                                                                              <  1:482042/150‑1 (MQ=255)
        aGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCggg                                                                                                                                              <  2:1598257/150‑1 (MQ=255)
        aGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCggg                                                                                                                                              <  2:1098707/150‑1 (MQ=255)
         ggAGCGTACCGAGAGGTGCAAAAAGCGATGAACGGGCGGTATCCCAAACCTGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCgggg                                                                                                                                             <  2:1979399/150‑1 (MQ=255)
          gAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGt                                                                                                                                            >  2:288594/1‑150 (MQ=255)
          gAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGt                                                                                                                                            >  2:220272/1‑150 (MQ=255)
            gCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGa                                                                                                                                          <  1:1407445/150‑1 (MQ=255)
            gCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCGGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGa                                                                                                                                          <  2:1894531/150‑1 (MQ=255)
              gTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAgg                                                                                                                                        <  2:1998895/150‑1 (MQ=255)
               tACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAggg                                                                                                                                       <  2:1929066/150‑1 (MQ=255)
                  cGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTGGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCAt                                                                                                                                    <  1:1057705/150‑1 (MQ=255)
                    tGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCa                                                                                                                                  <  2:2720332/150‑1 (MQ=255)
                       ggTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAgcg                                                                                                                               >  1:622564/1‑150 (MQ=255)
                       ggTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAgcg                                                                                                                               >  1:622628/1‑150 (MQ=255)
                        gTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAgcgc                                                                                                                              >  2:1644934/1‑150 (MQ=255)
                        gTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAgcgc                                                                                                                              >  2:1644030/1‑150 (MQ=255)
                          gCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAgcgcgc                                                                                                                            >  2:839405/1‑150 (MQ=255)
                          gCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAgcgcgc                                                                                                                            >  1:1166002/1‑150 (MQ=255)
                           cAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCa                                                                                                                           >  1:847580/1‑150 (MQ=255)
                            aaaaaGAGATTAATGGGCTTTATCCCAACATTGTCTGGCCGGACGACCCGGAAAATACTGAACCGACGGGACGGACTAAAAATTATTCTTATGGTTGG‑TTTT‑CGCCATATCCCGGTGGTAGTGGGCGGGGTTTTGGGCATCAGCGCGCAc                                                                                                                          <  2:2552139/150‑1 (MQ=255)
                            aaaaaGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTAGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCAc                                                                                                                          <  1:803029/150‑1 (MQ=255)
                             aaaaGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGACAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGAGCCGGGGTGAGGGCATCAGCGCGCACg                                                                                                                         >  2:1360360/1‑150 (MQ=255)
                              aaaGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGt                                                                                                                        >  1:1129346/1‑150 (MQ=255)
                               aaGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCCGCGCGCACGtg                                                                                                                       >  2:41325/1‑149 (MQ=255)
                                agagATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACgggc                                                                                                                      >  1:1457954/1‑147 (MQ=255)
                                    aTGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAATAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGttgtccc                                                                                                                  >  2:1841751/1‑145 (MQ=255)
                                     tGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGtgggcccg                                                                                                                 >  1:2451197/1‑143 (MQ=255)
                                     tGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGtgggcccc                                                                                                                 >  1:1972691/1‑143 (MQ=255)
                                        acgggCTTTAGCCAAAACATGTCGGGCCGCCCGACCGGGCAAATACTGCCCCGACGCGAGGGACTAAAAAGTTTTCGTAAGGTTGG‑TTTT‑CCCCCTCCCCCGGTGGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccctcac                                                                                                              <  2:1793015/148‑1 (MQ=25)
                                            gCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtgacccacaaccaa                                                                                                          >  1:1858371/1‑148 (MQ=255)
                                             ctttATCCAAAACATGTCTGGCCGGACCACCCGCAAAATACTGCACCGACGCGACGCACTAAAAATTTTTCTTATGTTTGG‑TTTT‑CTCCCACCCCCTGTGGGTGTGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTaa                                                                                                         <  2:874755/148‑1 (MQ=255)
                                                    ccAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGTGACCCGCCCCCCAAcacccac                                                                                                  >  2:1968101/1‑144 (MQ=37)
                                                     cAACCATGTCGGGCCGGACGACCCGGAAATTACTGCCCCGACGCGAGGGACGAAAATTTATTCTTATGGTTGG‑TTTT‑CTCCCTCCCCGGGTGGGAGTGGGCGGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTcc                                                                                                 <  2:1434115/150‑1 (MQ=255)
                                                     cAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTGGCCCGCAACCCAAcacacacc                                                                                                 >  1:2435407/1‑143 (MQ=37)
                                                     aaaaCTTGTCGGGCCGGACGACCCGGAAATAACTGCACCTAGGCGCCGGAGTAAAAAGTTTTCTTAAGGTTGT‑TTTT‑CCCCCTCTCCCTGTGGTAGTGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTcc                                                                                                 <  2:1434105/149‑1 (MQ=255)
                                                      aaaCATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGGCCCCCCCCCAAACCCACAccc                                                                                                >  1:2433430/1‑150 (MQ=37)
                                                       aaCTTGTGTGGCGGGACGCCCCGCAAAATCCGGCCCCGACGCTCCGTACTAAAATGTTTTGTTATGGTTGT‑TTTT‑CGCCCCCGCCGTGGGGGTGTGGGCGGGGTTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCt                                                                                               <  1:2182071/150‑1 (MQ=255)
                                                       aaCATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGACCCACACCCGAAACCACAccca                                                                                               >  2:2568444/1‑149 (MQ=37)
                                                           tttggggCCGGACTCCCCTGAAAATACTGCCCCGACGCCACGTACTAAAATTTATTCTTATGTTTGT‑TTTT‑CTCCACCGCCCTGTGGTTGTGGGCGGGGGTTCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAg                                                                                           <  2:48062/145‑1 (MQ=255)
                                                           gttCTGGCCGTACTACCCGGAAAATATGGCACCGACGCTACGTAGGAAAATTTTTTCGTACGGTTGT‑TTTT‑CTCCATCACCCTGTGGGTTTGGGCCGGGGTGTGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAg                                                                                           <  2:2090411/148‑1 (MQ=255)
                                                            gTCGGGCCGGACGACCCGGCAAATCCTGCACCGCCGCGCCGGACGAAAACGTATACGTATGGTTGG‑TTTT‑CGCCCCCCCCCTGTGGGTGCGGGCCGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAgg                                                                                          <  2:1965762/150‑1 (MQ=255)
                                                                 gccggccgACCCGCCAAATACGGCACCGACGCGACGTACTAAAAAGTTTGCGTACGGTTGT‑TTTT‑CTCCCGCTCCCTGGGGGAGCGGGCCGGGGTGTGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGagag                                                                                     <  2:1059190/150‑1 (MQ=255)
                                                                 gCCGTACGCCCCGCAAAATACGCCCCCGCCGCGACGGACTAAAAATTATCCTTATGGTTGG‑TTTT‑CTCCCACCCCCTGGGGGAGCGGGCCGGGGTGCGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTTGAGGGagag                                                                                     <  2:70993/150‑1 (MQ=255)
                                                                  ccGGCCGCCCCGCAAATTACTCCCCCGCCGCCACGCACTAAAATTTTTTC‑TAATGTTGGTTTTT‑CACCCCCCCCCTGGGGGTGCGGGCGGGGGTGCGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAgg                                                                                    <  1:2568444/150‑1 (MQ=255)
                                                                  ccGGACGACCCGGCAAATCCGGCACCGACGCGACCGACGAAAAAGTATTCGCAAGGTTGG‑TTTT‑ATCCCTCTCCCTGTGGGAGTGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAgg                                                                                    <  2:1446060/150‑1 (MQ=255)
                                                                     gcccacCCGCAAAATACTGCCCCCCCGCGACGGACTAAAAATTATTC‑TTATGTTGG‑TTTTACCCCCACCCCGTGGGGGCGCGGGCGGGGGTGCGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGa                                                                                 <  1:2486083/146‑1 (MQ=255)
                                                                       cgccccGGCAATTCCTGCACCGACGCTACGGACTAAAAAGTTTTCTTAAGGTTGG‑TTTG‑CCCCATCTCCCTGTGGGTGAGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTTGAGGGAGAgggttag                                                                               <  2:2482548/147‑5 (MQ=255)
                                                                       cgccccGGCAAATACGCCACCGCCGCGACGTACTAAAAAGTTTTGTTACGGTTGT‑TTTT‑CTCCCTCTCCCTGTGGGTGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTTGAGGGAGAgggttag                                                                               <  2:2482569/147‑5 (MQ=25)
                                                                          cccGCCAAATCCTCCCCCGCCGCCCCGGCCCAAAAAGTATTCGTAAGGTTGG‑TTTT‑CCCCCTCGCCCGGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGAt                                                                            <  2:1726542/150‑1 (MQ=37)
                                                                           ccgtaaaaTACTCCACCGACGCTACGTACTAAAATTTTTTCTTATGGTTGT‑TTTA‑CCCCCTCCCCCTGTGGGTGTGGGCGGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATc                                                                           <  1:1671395/145‑1 (MQ=255)
                                                                           ccGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTACGGTTGT‑TTTT‑CTCCCTCCCCCGGGGGGCGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATc                                                                           <  2:1087100/150‑1 (MQ=39)
                                                                           ccGGAAAATACTGACCCCACGCCACGGCCTAAAAAGTATTCTAAGGGTTGG‑TTTT‑CCCCCGCTCCCTGGGGGCGAGGGCCGGGGTGAGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATc                                                                           <  2:2386502/150‑1 (MQ=255)
                                                                             ggaaaaTACGCCCCCGACGCGACGGACGAAAAAGTATTCGTATGGTTGG‑TTTT‑CGCCCCCTCCCTGGGGGAGTGGGCCGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGa                                                                         <  2:2112108/147‑1 (MQ=255)
                                                                             gcaaaaTACTCCCCCGCCGCGACGGACGAAAAATTATTGGTATGGTTGT‑TTTT‑CACCCTCCCCCTGTGGGCGTGGGCCGGGGTGCGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGa                                                                         <  1:2650151/147‑1 (MQ=255)
                                                                              taaaaTACTGCACCGAGGCGAGGGACTAAAATTTTTTGTTATGGTTGG‑TTTT‑CCCCCTCGCCGTGTGGTTGTGGGCGGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAg                                                                        <  1:1757154/148‑1 (MQ=255)
                                                                               aaaaTCCTCCCCCGCCGCCCCGGACCAAAAAGTATCCGAATGGTTGT‑TTTT‑CCCCCCCCCCCTGGGGGTGCGGGCGGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGc                                                                       <  2:847580/149‑1 (MQ=255)
                                                                                        ccccgccgCTACGCACAAAAATTTA‑TCGTTAGGGTGGTTTTT‑CCCCCCCCCCGGGGGGGTGCGGGCGGGGGGGTGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAAtttt                                                              <  1:81668/148‑1 (MQ=255)
                                                                                        ccccgccgCGCCGGACTAAAATGTTTTCTTATGTTGGT‑TTTT‑CACCGAACCCCTGGGGTAGTGGGGGGGGGTGTGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAAtttt                                                              <  2:1193014/148‑1 (MQ=255)
                                                                                        cACCGCCGCGACGGACTAAAATGTTTTCGTATGGTTGG‑TTTT‑CTCCCACGCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAAtttt                                                              <  2:2179571/150‑1 (MQ=38)
                                                                                           cgacgcgccgGACTAAAAAGTTTTCGTATGGTTGG‑TTTT‑CCCCCTCTCCCTGTGGGTGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTgag                                                           <  2:2724096/150‑1 (MQ=38)
                                                                                               gcgacgGACTAAAAATTATTCGTATGGTTGT‑TTTT‑CTCCCTCTCCCTGTGGGTGTGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGat                                                       <  1:2721698/150‑1 (MQ=38)
                                                                                               gccacgtactAAAAATTTTTCTTATGGTTGG‑TTTA‑CGCCCTCCCCGGGGGGTTGTGGGCGGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGat                                                       <  1:2471749/147‑1 (MQ=255)
                                                                                                 gccggactAAAAATTTTTCGTACGGTTGG‑TTTT‑CTCCCCCGCCCTGGGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCACCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGatat                                                     <  2:2599962/148‑1 (MQ=38)
                                                                                                  ccggactAAAAAGTTTTCGTATGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGTGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtc                                                    <  2:1090423/149‑1 (MQ=38)
                                                                                                    ggacgAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTGGCCCCCACCCCAACCCCCACCCCCCCGGGGGCGGGGGCCGCTGGCGCCCAAAAATTTGGAGATAtatt                                                  >  1:2479045/1‑150 (MQ=16)
                                                                                                              ttATTCGTATGGTTGG‑TTTT‑CTCCCTCTCCGTGTGGGAGAGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCtt                                        <  2:1825242/149‑1 (MQ=255)
                                                                                                                tttctaaaGGTTGT‑TTTA‑CCCCCGCCCCCGGTGGGAGCGGGCCGGGGTTAGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGt                                      <  2:554555/144‑1 (MQ=255)
                                                                                                                tttCGTATGGTTGG‑TTTT‑CTCCCTCTCCGTGTGGGTGTGGGCGGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGt                                      <  2:2451197/149‑1 (MQ=255)
                                                                                                                  tcttttggttgt‑ttTT‑CGCCCCCTCCGTGTGGGTTTGGGCCGGGTTGTGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTaa                                    <  1:41325/144‑1 (MQ=255)
                                                                                                                  tccttcggttgt‑ttTC‑CGCCCCCCCCCTGTGGTCTCGGGCGGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTaa                                    <  1:1208422/144‑1 (MQ=255)
                                                                                                                  tCGTAAGTTTGG‑TTTT‑CTCCCACTCCCTGGGGGAGAGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTaa                                    <  2:870392/150‑1 (MQ=255)
                                                                                                                  tCGTAAGGTTGG‑TTTT‑CGCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTaa                                    <  2:1822037/150‑1 (MQ=255)
                                                                                                                   cGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAAc                                   <  2:2581350/150‑1 (MQ=255)
                                                                                                                   cGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAAc                                   <  2:2581552/150‑1 (MQ=255)
                                                                                                                           tttttttt‑CTCCATCTCCCTGTGGTTTTGGGCGGGGGTGCGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAgaagaa                            <  1:563238/146‑1 (MQ=255)
                                                                                                                                       cctctcccgGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTTTGTCTTGTAACAGAAGAACAGAAAATCgg                 <  2:2128600/150‑1 (MQ=255)
                                                                                                                                        ctctccctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCACCCCCAAAACCACTCCCTCAAGGGGGGGGGGGCCGAGAGAGCCCCAAATTTGTGGGTTATATTATTATGTGTTTTTTCAGAAGAACAGGAAATAggg                >  2:1496340/1‑150 (MQ=12)
                                                                                                                                             cgtgtgggtgtgggCGGGGGTGCGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCtttt           <  1:169509/148‑1 (MQ=255)
                                                                                                                                                   ggAGAGAGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCt     <  1:1360360/150‑1 (MQ=255)
                                                                                                                                                      gagGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGaa  <  2:2134763/150‑1 (MQ=255)
                                                                                                                                                       |                                                                                                                                                    
TTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAA  >  NC_000913/164415‑164712

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑