Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913159,1010CA27.6% 440.5 / 56.2 199C9F (TGC→TTC) pcnBpoly(A) polymerase I
Reads supporting (aligned to +/- strand):  ref base C (107/37);  new base A (0/55);  total (107/92)
Fisher's exact test for biased strand distribution p-value = 1.27e-24
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 206 total aligned reads displayed.

CGCTTTATTGAGCCTGTACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGGTAAGCCGC  >  NC_000913/158953‑159249
                                                                                                                                                    |                                                                                                                                                    
cGCTTTATTGAGCCTGTACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGc                                                                                                                                                      >  1:758112/1‑149 (MQ=255)
 gCTTTATTGAGCCTGTACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCa                                                                                                                                                     >  1:1152195/1‑149 (MQ=255)
 gCTTTATTGAGCCTGTACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCa                                                                                                                                                     >  1:2834869/1‑149 (MQ=255)
     tATTGAGCCTGTACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCaaaaa                                                                                                                                                 <  2:2687541/149‑1 (MQ=255)
       ttGAGCCTGTACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAAtt                                                                                                                                               <  2:2087864/149‑1 (MQ=255)
       ttGAGCCTGTACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAAtt                                                                                                                                               >  2:1130181/1‑149 (MQ=255)
        tGAGCCTGTACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTa                                                                                                                                              <  2:784255/149‑1 (MQ=255)
           gCCTGTACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCg                                                                                                                                           <  2:2762052/149‑1 (MQ=255)
           gCCTGTACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCg                                                                                                                                           >  1:2631593/1‑149 (MQ=255)
            ccTGTACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGa                                                                                                                                          >  1:307139/1‑149 (MQ=255)
                 aaaTTACCTTCAGGGAATTTTCAATTATATATTGGGGGTAAATAGCTTGCGGCACCCGGGGGATCACCGTCACCTGTGGACGGGCGACGGCCTGTCCCGCCTCGCTTTCCGCGCGGCTTACCACCTTGCGGCAAAAATTCGCGACTCgg                                                                                                                                     <  2:2972587/147‑1 (MQ=255)
                    ttACCTTCAGGGCATTTTCACTGATATCTTTGCGGTAAATAGCATGCTGCACCCGCGGGAACACCGACACCGGTGGACGGGCGACTGCCTGTACAGCCTCGCTTTCCCCGCGGATTAGCACCTTGCGGAAAAAATTCGCGACTCGGGTa                                                                                                                                  <  2:1554665/149‑1 (MQ=255)
                    ttACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTa                                                                                                                                  >  1:2749561/1‑149 (MQ=255)
                     tACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTaa                                                                                                                                 >  1:2887587/1‑149 (MQ=255)
                     tACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTaa                                                                                                                                 >  1:1770516/1‑149 (MQ=255)
                       ccTTCCGGGAATTTTCACTTATATCTTTGCGGTAAATAGCATGCTGCACACGCGGTATCACCGTCACCTGGGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGAAAAAATTCGCGACTCGGGTaaaa                                                                                                                               <  1:2311070/149‑1 (MQ=255)
                         ttCTGGGCATTTTCACTTATATCTTTGCGGGAAATAGCTTGGTGCTCACGCGGTATCACCGTCACCTGTGGAGGGGCGACTGCCTGTTCAGCCTCGTTTTCCTCGGGGATTAGCACCTTGCGGAAAAAATTAGCGACTCGGGTAAAAAt                                                                                                                             <  2:219516/149‑1 (MQ=255)
                         ttCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTAAAAAt                                                                                                                             >  1:1001194/1‑149 (MQ=255)
                         ttCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTAAAAAt                                                                                                                             >  1:3012141/1‑149 (MQ=255)
                          tccgggCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCACACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTACAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGAAAAAATTAGCGACTCGGGTAAAAATa                                                                                                                            <  2:750848/146‑1 (MQ=255)
                           cAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTCAAAATat                                                                                                                           >  2:1300327/1‑148 (MQ=255)
                               gCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTAAAAATAGTaca                                                                                                                       >  1:1861989/1‑149 (MQ=255)
                                cATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTAAAAATAGTacac                                                                                                                      >  1:2449494/1‑149 (MQ=255)
                                    ttCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTAAAAATAGTACACCTCg                                                                                                                  >  2:2396338/1‑149 (MQ=255)
                                    tacACTGATATCTTTGCGGGAAAGAGCATGCTGCACCCGCGGGATCACCGTCCCCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTAAAAATAGTACACCTCg                                                                                                                  <  2:1726628/147‑1 (MQ=255)
                                          gATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTAAAAATAGTACACCTCGGTAgtg                                                                                                            >  2:660472/1‑149 (MQ=255)
                                              tCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCaa                                                                                                        >  1:2273419/1‑149 (MQ=255)
                                               cTTGGCGGTAAATAGCATGCGGCACACGCGGTATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCaaa                                                                                                       <  2:134982/149‑1 (MQ=255)
                                                 ttGCGGGAAATAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAAca                                                                                                     >  1:716549/1‑149 (MQ=255)
                                                     gggAAATAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAAcatcat                                                                                                 >  1:1742289/1‑149 (MQ=255)
                                                      gtaaaTAGATTGCTGCTCACGGGTGATCACCGTCACGTGGGGAGGGGCGACTGCCTGTTCAGCCTGGATTTCCTCGCGGCTTAGCACTTGGCGTAAAAAATACGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCAtt                                                                                                <  1:2578090/147‑1 (MQ=255)
                                                       taaaTAGAATGCGGCACACGCGGTATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGAAAAAATTAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTc                                                                                               <  2:1625444/148‑1 (MQ=255)
                                                       gAAATAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTc                                                                                               >  1:2188402/1‑149 (MQ=255)
                                                       gAAATAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTc                                                                                               <  2:2749561/149‑1 (MQ=255)
                                                             gCATGCTGCTCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACa                                                                                         >  2:2103670/1‑149 (MQ=255)
                                                              cttgcggcTCCCGGGGTATCCCCTTCACTTGTGGCCGGGCGACGGCTTGTTCAGCCTCGTTTTCCTCGGGGTTTAGCACCTTGCGTAAAAATTTAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACaa                                                                                        <  1:111487/147‑1 (MQ=255)
                                                               aTGCTGCTCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACaaa                                                                                       >  2:1785129/1‑149 (MQ=255)
                                                                 gctgctCCCGGGGTATCACCGTCACCTGTGGACGGGCGACTGCCTTTACAGCCTCGCTTTCCTCGCGGATTAGCACCTTGCGGAAAAAATTCGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACaaaaa                                                                                     <  2:414496/149‑1 (MQ=255)
                                                                  ctgctCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACaaaaaa                                                                                    >  1:479582/1‑149 (MQ=255)
                                                                    gctCCCGGGGTATCACCTACACCTGTGGAGGGGCGACTGCTTTTTCAGCCTCGTTTTCCTCGGGGTTTAGAACCTTGGGGAAAAAATAAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAAt                                                                                  <  1:763512/149‑1 (MQ=255)
                                                                     ctCACGGGTGATCACCTTCACGTGTGGTGGGGGTACTGCTTTTTCAGCCTCGTTTTCCTCGCGGTTTAGCACGTTGCGGAAAATATTAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATa                                                                                 <  1:2084831/149‑1 (MQ=255)
                                                                     ctCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATa                                                                                 >  2:1383063/1‑149 (MQ=255)
                                                                     ctCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTACAGCCTCGCTTTCCTCGCGGCTTAGAACCTTGCGGAAAAAATTAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATa                                                                                 <  2:1029375/149‑1 (MQ=255)
                                                                      tCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAg                                                                                >  1:26369/1‑149 (MQ=255)
                                                                      acACGCGGTATCCCCGTCACGTGTGGAGGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGGGGCTTAGCACCTTGCGGAAAAAATTAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAg                                                                                <  2:339809/148‑1 (MQ=255)
                                                                        aCGCGGGATCACCGTCACCTGTGTAGGGGGTAGTGACTGTTCAGCCTCTCTTTCCTCGGGTCTTACCATTTTGCGGCAAAATTTAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCg                                                                              <  2:404592/149‑1 (MQ=255)
                                                                         cgcgGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCgg                                                                             >  2:1906079/1‑149 (MQ=255)
                                                                           cgGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCt                                                                           >  2:2562326/1‑149 (MQ=255)
                                                                                tCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCa                                                                      >  2:720748/1‑149 (MQ=255)
                                                                                   ccGTCACCTGGGGAGGGGCGACTGCCTTTTCAGCCTCGCTTTCCTCGCGGTTTCGCACCTTGCGTAAAAAATAAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAg                                                                   <  1:338370/149‑1 (MQ=255)
                                                                                    cGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGc                                                                  >  2:174011/1‑149 (MQ=255)
                                                                                    cGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGc                                                                  >  1:1000835/1‑149 (MQ=255)
                                                                                        aCCTGTGGACGGGCGACGGCTTGTTCAGCCTCGTTTTCCTCGCGGATTAGCACCTTGCGGAAAAATTTAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAg                                                              <  1:2510732/149‑1 (MQ=255)
                                                                                           tgtgGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCAt                                                           >  2:2365105/1‑149 (MQ=255)
                                                                                           tgtgGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCAt                                                           >  2:181411/1‑149 (MQ=255)
                                                                                                   ggCTACTGCGTTTACAGCCTCGATTTCCTCGCGGATTCGACCCTTGCGGAAAAAATTCGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCAtt                                                   <  1:784093/149‑1 (MQ=255)
                                                                                                   ggCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCAtt                                                   >  2:856447/1‑149 (MQ=255)
                                                                                                     cGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTg                                                 >  1:520637/1‑149 (MQ=255)
                                                                                                     cGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTg                                                 >  1:336479/1‑149 (MQ=255)
                                                                                                        cTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTAAAAATAGGACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTgaga                                              >  2:214105/1‑149 (MQ=255)
                                                                                                        cTGCCGGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGAAAAAATTAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTgaga                                              <  2:2785869/149‑1 (MQ=255)
                                                                                                         tGCCTTTACAGCCTCGCTTTCCTCGCGGATTAGCACCTTGCGGAAAAAATTAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGaa                                             <  1:2039301/149‑1 (MQ=255)
                                                                                                          gCCTGTTCAGCCTCGCTTTCCTCGCGGCTTCGCACCTGGCGGAAAAAATCAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAAt                                            <  1:660472/149‑1 (MQ=255)
                                                                                                            cTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGt                                          >  1:2700495/1‑149 (MQ=255)
                                                                                                            cTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGt                                          >  1:1818515/1‑149 (MQ=255)
                                                                                                            atttacAGCCTCTCTTTCCCGGCGGGTAATCACGTGGCGGAAAAATTAAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGt                                          <  2:1296758/144‑1 (MQ=255)
                                                                                                             tGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATTGCTCAGCATGAAGCCTTTGATAATGtt                                         >  2:2673936/1‑149 (MQ=255)
                                                                                                              gtaccgCCTCGCTTTCCTCGCGGCTTACCACCTTGCGGCAAAAATTCGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTg                                        <  2:1192572/144‑1 (MQ=255)
                                                                                                              gTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTg                                        <  2:608684/149‑1 (MQ=255)
                                                                                                               ttCAGCCTCGGTTTCCGCGCGGCTTAGAACCTTGCGCAAAAAATTAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGa                                       <  2:8695/149‑1 (MQ=255)
                                                                                                                tCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTCAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATAATAGCTCAGCATGACGCATTTGAGAATGTTGaa                                      >  1:90892/1‑149 (MQ=255)
                                                                                                                tCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGAAAAAATTAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGaa                                      <  2:2518033/149‑1 (MQ=255)
                                                                                                                tCAGCCTCGCTTTCATCGCGGCTTCGCCCCTTGCGGCAAAATTTAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGaa                                      <  2:2517464/149‑1 (MQ=255)
                                                                                                                tCAGCCCCGCTTTCCTCGCGGCTTCGCACCTTGCGGAAAAAATCCGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGaa                                      <  2:758191/149‑1 (MQ=255)
                                                                                                                   gCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAAttt                                   >  2:444063/1‑149 (MQ=255)
                                                                                                                   gCCTCGCTGACCCCGCGGCTTAGCACCTGGCGGCAAAAATTAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAAttt                                   <  1:1413132/149‑1 (MQ=255)
                                                                                                                    ccTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTa                                  >  2:2074873/1‑149 (MQ=255)
                                                                                                                     cTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGTCGCATTTGAGAATGTTGAATTTAc                                 >  2:1823365/1‑149 (MQ=255)
                                                                                                                     cTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTAc                                 >  2:2490297/1‑149 (MQ=255)
                                                                                                                     cTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTAc                                 >  1:2647486/1‑149 (MQ=255)
                                                                                                                     cTCGCTTTCCACGCGGCTTAGCACATTGCGTCAAAAATTAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTAc                                 <  1:2325923/149‑1 (MQ=255)
                                                                                                                      tCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCCTGACGCATTTGAGAATGTTGAATTTACa                                >  2:2883904/1‑149 (MQ=255)
                                                                                                                      tCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACa                                >  2:1929199/1‑149 (MQ=255)
                                                                                                                       cGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACaa                               >  1:1573588/1‑149 (MQ=255)
                                                                                                                         cTTTCCTCGCGGCTTAGCACCTTGCGGCAAACATTAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCCTTTGAGAATGTTGAATTTACAAtt                             >  2:3062612/1‑149 (MQ=255)
                                                                                                                           ttCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTAAAAATAGTACACCTCGGTAg                                                                                                              >  1:2112900/1‑66 (MQ=255)
                                                                                                                            tCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGcc                          >  2:2187918/1‑149 (MQ=255)
                                                                                                                               tCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGAc                       >  1:986834/1‑149 (MQ=255)
                                                                                                                                cgcgGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACt                      >  2:1041618/1‑149 (MQ=255)
                                                                                                                                 gcgGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTc                     <  2:1598139/149‑1 (MQ=255)
                                                                                                                                    gCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCggg                  <  2:508932/149‑1 (MQ=255)
                                                                                                                                          cACCTTGCGGCAAAAATTAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCAcggc            >  1:539758/1‑149 (MQ=255)
                                                                                                                                          cACCTTGCGGAAAAAATTAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCAcggc            <  1:2919277/149‑1 (MQ=255)
                                                                                                                                            ccTTGCGGCAAAAATTAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCAcggcgg          >  1:2957490/1‑149 (MQ=255)
                                                                                                                                            ccTTGCGGAAAAAATTAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCAcggcgg          <  1:2183512/149‑1 (MQ=255)
                                                                                                                                               tGCGGCAAAAATTAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGGTaa       >  1:1472663/1‑149 (MQ=255)
                                                                                                                                                 cGGCAAAAATTAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGGTAAgc     <  2:2188402/149‑1 (MQ=255)
                                                                                                                                                   gCAAAAATTAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGGTAAgccg   <  2:2449494/149‑1 (MQ=255)
                                                                                                                                                    cAAAAATTAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGGTAAgccgc  >  2:478300/1‑149 (MQ=255)
                                                                                                                                                    cAAAAATTAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGGTAAgccgc  >  1:1296335/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CGCTTTATTGAGCCTGTACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGGTAAGCCGC  >  NC_000913/158953‑159249

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑