Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913361,3110CA27.6% 506.7 / 28.7 239E184* (GAA→TAA) lacAgalactoside O‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base C (52/116);  new base A (64/0);  total (123/116)
Fisher's exact test for biased strand distribution p-value = 5.11e-25
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 243 total aligned reads displayed.

CGCTTTGTTCATGCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCA‑GCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGATCCAGACGTTATTGCC  >  NC_000913/361164‑361458
                                                                                                                                                   |                                                                                                                                                    
cgcTTTGTTCATGCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTcg                                                                                                                                                     <  1:1860199/149‑1 (MQ=255)
 gcTTTGTTCATGCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTcgc                                                                                                                                                    <  2:2927251/149‑1 (MQ=255)
   tttGTTCATGCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTTTTTAGCGa                                                                                                                                                  >  1:839002/1‑149 (MQ=255)
         cATGCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAAcc                                                                                                                                            <  1:1904853/149‑1 (MQ=255)
              cGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACa                                                                                                                                       <  1:2890929/149‑1 (MQ=255)
               ggATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACaa                                                                                                                                      <  2:1550186/149‑1 (MQ=255)
                gATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTGTTTTTAGCGTATAAACCGACAAg                                                                                                                                     >  1:1590983/1‑149 (MQ=39)
                  tGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGa                                                                                                                                   <  2:827641/149‑1 (MQ=255)
                   gCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGaa                                                                                                                                  <  2:670088/149‑1 (MQ=255)
                      gCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGc                                                                                                                               <  1:3080765/149‑1 (MQ=255)
                      gCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGCGAATAAACCGACCACGAACGc                                                                                                                               >  2:218886/1‑149 (MQ=255)
                             tAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCA‑gccgc                                                                                                                       <  1:2571670/149‑1 (MQ=255)
                                aTCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAAACCCACAAGGAACCCCA‑GCCGCCac                                                                                                                    >  1:1965815/1‑149 (MQ=255)
                                 tCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCA‑GCCGCCacg                                                                                                                   >  2:889139/1‑149 (MQ=255)
                                 tCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCAAATAACCCGACAAGGAACGCCA‑GCCGCCacg                                                                                                                   >  2:1786436/1‑149 (MQ=255)
                                 tCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGCGCAAAAACAGACAACGAAAGCCC‑GCCCCCacc                                                                                                                   >  1:2509017/1‑148 (MQ=38)
                                  cGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCA‑GCCGCCacga                                                                                                                  >  2:1174779/1‑149 (MQ=255)
                                    cTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGCGAATAAACCCACAAGGAAAGCCA‑GCCGCCacgacg                                                                                                                >  1:418529/1‑149 (MQ=255)
                                      gAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTTTTTAGCGAATAACCCGACAAGGAACGCCA‑GCCGCCACGACGtt                                                                                                              >  2:167098/1‑149 (MQ=255)
                                        aCTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCA‑GCCGCCACGACGTTtg                                                                                                            <  2:2709864/149‑1 (MQ=255)
                                         cTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCA‑GCCGCCACGACGTTtgg                                                                                                           <  1:1636685/149‑1 (MQ=255)
                                         aaaaattgCCTGATACGCTGCGCTTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATATCCCGACAAGGAACGCCA‑GCCGCCACGACGGTtgg                                                                                                           >  2:3007945/8‑149 (MQ=255)
                                           tGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCA‑GCCGCCACCACGTTtggtg                                                                                                         >  1:2986967/1‑149 (MQ=255)
                                           tGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTGTATTTAGCGAATAACCCGACAAGGAACGCCA‑GCCGCCACGACGGTtggtg                                                                                                         >  1:1427377/1‑149 (MQ=255)
                                              aGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCA‑GCCGCCACGACGTTTGGTGGaa                                                                                                      <  1:2591033/149‑1 (MQ=255)
                                                gCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTGTATTTAGCGAATAACCCCACAAGGACACCCA‑CCCGCCCCGACCGTTGGTGGaaag                                                                                                    >  1:1987920/1‑147 (MQ=255)
                                                 ccTGATACGCTGCGCTTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTTTTTAGGGGATAAACCCACAAAGAACGACA‑GCCCCCACGAACTTTTGTTGAAttt                                                                                                   >  1:1112839/1‑147 (MQ=255)
                                                 ccTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAAACCGACAAGGAACGCCA‑GCCGCCACGACGGTTGGTGGAATGt                                                                                                   >  2:189800/1‑149 (MQ=255)
                                                 ccTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTGTTTTTAGCGTATAACCCGCCCAGGAACGCCA‑GCCCCCCCGACGTGTGGGGGGATGt                                                                                                   >  1:1119471/1‑149 (MQ=255)
                                                    gATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCA‑GCCGCCACGACGTTTGGTGGAATGTCtt                                                                                                <  1:1340261/149‑1 (MQ=255)
                                                    gATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCA‑GCCGCCACGACGTTTGGTGGAATGTCtt                                                                                                <  2:2680106/149‑1 (MQ=255)
                                                    gATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCA‑GCCGCCACGACGTTTGGTGGAATGTCtt                                                                                                <  1:712032/149‑1 (MQ=255)
                                                    gATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAAATGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTTTTTTTCGAAAAAACCCACAAAGAAACCCACCCCCCCCCAACGTTTGTTGGatgtttt                                                                                                 >  1:182705/1‑143 (MQ=255)
                                                     aTAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCA‑GCCGCCACGACGTTTGGTGGAATGTCttt                                                                                               <  2:24646/149‑1 (MQ=255)
                                                     aTAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCA‑GCCGCCACGACGTTTGGTGGAATGTCttt                                                                                               <  2:437050/149‑1 (MQ=255)
                                                      tAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCA‑GCCGCCACGACGTTTGGTGGAATGTCtttt                                                                                              <  2:2270058/149‑1 (MQ=255)
                                                      tAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGCGAATAACCCCACAAGGAACGCCA‑GCCGCCACCACGTTTGGTGGGATGTCtttt                                                                                              >  1:2251115/1‑149 (MQ=255)
                                                       aaGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCA‑GCCGCCACGACGTTTGGTGGAATGTCTTTtg                                                                                             <  2:2112313/149‑1 (MQ=255)
                                                        aGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTGTATTTGGCGAATAAACCGACAAGGAAAGCCA‑CCCGCCACGACGTTTGGTGGAATGTCTTTtgt                                                                                            >  2:1285914/1‑149 (MQ=255)
                                                          cgcAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCA‑GCCGCCACGACGTTTGGTGGAATGTCTTTTGTGa                                                                                          <  2:1742804/149‑1 (MQ=255)
                                                          cgcAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGCGAATAAACCGACAAGGAACCCCA‑GCCGCCACGACGTTTGGTGGAATGTGTTTTGTGa                                                                                          >  1:630306/1‑149 (MQ=255)
                                                              gCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCCACAAGGAACGCCC‑GCCCCCACCCCCGTTGGGGGGATGTCTTTTTTGTCGAt                                                                                      >  1:344688/1‑149 (MQ=255)
                                                                   tCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGCGAATAAACCCACACGGAAACCCA‑CCCCCCCCCACCGTTTGTGGAAATTGTTTTTTGTCGataataa                                                                                 >  1:2032337/1‑148 (MQ=255)
                                                                      ggCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGCGAATAAACCCACAACGAACCCCA‑GCCCCCACGACGGTTGGTGGTATGTCTTTTTTGACGATACTAaccc                                                                              >  1:2671377/1‑148 (MQ=255)
                                                                        cAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTTTTTCGCGAAAAACCCCACAAGGAAACCCA‑GCCGCCACGACGTTTGGTGGTAAGTGTTTTTTGAAGATAATAaccccc                                                                            >  1:1738460/1‑146 (MQ=255)
                                                                        cAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGTATAACCCGACAAGGAACGCCA‑GCCGCCACGGCGGTTGGTGGGATGTGTTTTGTGTCGATACTACCcgcg                                                                            >  2:1028259/1‑149 (MQ=255)
                                                                        cAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCA‑GCCGCCACGACGTTTGGTGGAATTTCTTTTGTGAAGATACAAACcgcg                                                                            >  2:2154893/1‑149 (MQ=255)
                                                                        cAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCA‑GCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCagcg                                                                            <  2:2780979/149‑1 (MQ=255)
                                                                        cAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGCGAATAAACCGACAAGGAAAGCCA‑GCCCCCACGACGTTTGGTGGTATGTGTTTTGTGAGGATACAACCcgcg                                                                            >  1:2502406/1‑149 (MQ=255)
                                                                          aTTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTTTTTAGCGAATAAAACCACAATTAAAACCC‑GCCGCCCACACCGTTGTGGGGATGTCTTTTTTGAAGAAAAAACCCCCGcc                                                                          >  1:1519321/1‑149 (MQ=255)
                                                                             tttATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCA‑GCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAAt                                                                       <  1:149272/149‑1 (MQ=255)
                                                                               tataATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGCGAATAACCCGACAAGGAAAGCCA‑GCCGCCACGACGTTTGGTGGAATGTATTTTGTGACGATTCTAACCCCGCCAaaaa                                                                     >  2:528791/1‑149 (MQ=255)
                                                                                ataATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCA‑GCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAAc                                                                    <  2:1472115/149‑1 (MQ=255)
                                                                                 taATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTTTTTGGCGAAAAAACCCCCAAGGCACGCCA‑GCCGCCCCCACGGTTTGTGGGATGTAGTTTTTTTCGATACTAACCCCGCCCAAAACa                                                                   >  1:1243114/1‑149 (MQ=255)
                                                                                  aaTTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCA‑GCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAg                                                                  <  2:1408758/149‑1 (MQ=255)
                                                                                   aTTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCA‑GCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGa                                                                 <  1:1245493/149‑1 (MQ=255)
                                                                                     ttAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCA‑GCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAAt                                                               <  2:742501/149‑1 (MQ=255)
                                                                                     ttAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTGTATTTCGCGGATAAACCCACAAGGAACGCCC‑GCCCCCCCCCCGGTTGGGGGGATGTCTTTTTTGTCGATACTACCCCCGCCCAAAACAGAAt                                                               >  1:1144948/1‑149 (MQ=255)
                                                                                        aaCTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCA‑GCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTAt                                                            <  2:2427252/149‑1 (MQ=255)
                                                                                         aCTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCA‑GCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATc                                                           <  2:1370816/149‑1 (MQ=255)
                                                                                         aCTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGCGAATAAACCCACAAAGAACCCCA‑GCCCCCACCACCTTTGGTGGAATGTGTTTTGTGTAGGTAATACCCCCCCCAAAAAAACAATTAta                                                           >  1:2406337/1‑148 (MQ=255)
                                                                                          cTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCA‑GCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATcc                                                          <  1:2140985/149‑1 (MQ=255)
                                                                                            gACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGCGAATAAACCGACAAGGAAAGCCA‑GCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTAACCCCGCCCATAACAGAATTATaccc                                                        >  2:970174/1‑149 (MQ=255)
                                                                                              cGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTTTTTATCGAATAAACCCCCAAAGAAACCCA‑CCCCCCCCCACGTGTGGTGGTATGTCTTTTGTTAAGAAACAAACCCCGCCAAAAACAGAAATaaaaccca                                                      >  1:174090/1‑142 (MQ=255)
                                                                                              cGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCA‑GCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGa                                                      <  2:1969243/149‑1 (MQ=255)
                                                                                                  tCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAAAAAACCCACAAGGAACGCCA‑GCCCCCACCACCTTTGGTGGAATGTCTTTTGTGACGATACAACCCCCGCCAAAAACAGAATTATACCCGATGGt                                                  >  2:708907/1‑149 (MQ=255)
                                                                                                    aaCTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCA‑GCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGa                                                <  2:166703/149‑1 (MQ=255)
                                                                                                     aCTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTTTTTAGCGAATAACCCCACAACGAACCCCA‑GCCGCCACCACGGTTGTTGGAATGTCTTTTGTTAAGATACTACCCCCGCCAAAAACAAAAATATACCCGATGGTTAc                                               >  2:1770497/1‑149 (MQ=255)
                                                                                                        ttATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTGGCGAATAAACCGACAAGGAACGCCA‑GCCGCCACGACGTTTGGTGGAATGTCTTTTGTGAAGATAATAACCGCGCCAATAACAGAATTTAACCCGATGGTGACGcc                                            >  2:2818531/1‑149 (MQ=255)
                                                                                                        ttATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCA‑GCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGcc                                            <  1:952325/149‑1 (MQ=255)
                                                                                                           taATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCA‑GCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTgg                                         <  2:319188/149‑1 (MQ=255)
                                                                                                            aaTCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGCGAATAACCCGACAAGGAACGCCA‑GCCGCCACGACGGTTGGTGGAATGTCTTTTGTGACGATACTACCCCCGCCAATAACAGAATTATACCCGATGGTGACGCCTGGa                                        >  2:2073364/1‑149 (MQ=255)
                                                                                                             aTCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCA‑GCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGAt                                       <  2:2291388/149‑1 (MQ=255)
                                                                                                             aTCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCA‑GCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTAACCGCGCCAATAACAGAATTATACCCGAAGGTGACGCCAGGAt                                       >  2:1705826/1‑149 (MQ=255)
                                                                                                              tCTTTGAAATAATAGTGCTTATCCCGGTCGTTTTTTTATCGAAAAAACAGACAAACTACGCCA‑GCCCCCACCAACGTTGTTGGAAATTAGATTTTGTCCAAAAAAACCCCGCCAATAAAAGAATTATCCCCGATGGTGACGcaagtatt                                      >  1:2916427/1‑142 (MQ=255)
                                                                                                               cTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCA‑GCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATta                                     <  1:595734/149‑1 (MQ=255)
                                                                                                                 ttGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAATGAAAGCCA‑ACCGCCACGACGTTTTGTGGTATTTGTTTTTTGACGATACTAACCCCGCCAATAACAGAATTATACCCCATGGTGACGCCGGGATtaat                                   >  1:2128298/1‑149 (MQ=255)
                                                                                                                 ttGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGCGAATAACCCGACAAGGAACGCCA‑GCCGCCAAGACGTTTGGTGGTATGTCTTTTGTGACGAAACTAACCGCGCCAAAAACAGAATTATACCCCATGGTGACGGCTGGCTtaat                                   >  2:2751875/1‑149 (MQ=255)
                                                                                                                 ttGAAATAATAGTGCTTATCCCGGTCGTGTATTTAGCGCAAAAACCCACAAGGAAACCCA‑CCCGCCACCACGGTTTGTGGGAATTATTTTTTGAAGATAAAAACCCCGCCCAAAAAAAAAATATACCCCAAGGTGTCGCCAGGTTtaat                                   >  1:522769/1‑149 (MQ=255)
                                                                                                                  tGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCA‑GCCGCCACGACGTTTGGTGGTATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATtaata                                  <  1:755787/149‑1 (MQ=255)
                                                                                                                   gAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCA‑GCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATtaataa                                 <  1:1296282/149‑1 (MQ=255)
                                                                                                                   gAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCA‑GCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATtaataa                                 <  2:3097865/149‑1 (MQ=255)
                                                                                                                   gAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCA‑GCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATtaataa                                 <  2:1678550/149‑1 (MQ=255)
                                                                                                                     aataatAGTGCTTATCCCGGTCGTTTATTTAGCGAATAACCCCACAAGGAACGCCC‑GCCGCCACCACGTTTTGTGGGATGTGTTTTGTGTCGATAAAAACCCCGCCCAAAAAAAAATTATCCCCGAGGGTGTAGCCAGGAGttataaac                               >  1:1798556/1‑147 (MQ=255)
                                                                                                                       taataGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCA‑GCCGCCACGACGGTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACcac                             >  2:2348883/1‑149 (MQ=255)
                                                                                                                       taataGTGCTTATCCCGGTCGTTTATTTAGCGAATAAACCGACAAGGAAAGCCA‑GCCGCCACGACGTTTTGTGGAATGTCTTTTGTGTAGATAAAACCCGCGCCAAAAACAGAATTATACCCGATGGTGTAGCCTGGATTAATAAacac                             >  2:1584626/1‑149 (MQ=255)
                                                                                                                        aataGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCA‑GCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACcaca                            <  2:2032337/149‑1 (MQ=255)
                                                                                                                           aGTGCTTATCCCGGTCGTTTTTTTCGCCGCTAAACCCACAACGTACGCCA‑GCCGCCCCGAGGTTTGGTGGTCGGTCGTTTGTGACGTTACTAACCGCGCCAAAAAAAGAATTATCCCCGAGGGTTACGCCTGGGTTAAAAAAcacaggt                         >  2:766436/1‑146 (MQ=255)
                                                                                                                           aGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCA‑GCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGa                         <  2:1847541/149‑1 (MQ=255)
                                                                                                                              gCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCA‑GCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACtt                      <  2:1125562/149‑1 (MQ=255)
                                                                                                                               cTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCA‑GCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTc                     <  2:1877843/149‑1 (MQ=255)
                                                                                                                               cTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCA‑GCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTc                     <  1:2801102/149‑1 (MQ=255)
                                                                                                                                  aTCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCA‑GCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGa                  <  1:2156036/149‑1 (MQ=255)
                                                                                                                                        gTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCA‑GCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGATCCAGa            >  2:3113487/1‑149 (MQ=255)
                                                                                                                                        gTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCA‑GCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGATCCAGa            <  2:348000/149‑1 (MQ=255)
                                                                                                                                         tCGTTTATTTCGCGAATAACCCGACAAGGAACGCCA‑GCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGATCCAGAc           <  1:2633773/149‑1 (MQ=255)
                                                                                                                                             ttATTTCGCGAATAACCCGACAAGGAACGCCA‑GCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGATCCAGACGTTa       <  2:58220/149‑1 (MQ=255)
                                                                                                                                             ttATTTCGCGAATAACCCGACAAGGAACGCCA‑GCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGATCCAGACGTTa       >  2:2214654/1‑149 (MQ=255)
                                                                                                                                              tATTTCGCGAATAACCCGACAAGGAACGCCA‑GCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGATCCAGACGTTAt      <  2:1890707/149‑1 (MQ=255)
                                                                                                                                                  tCGCGAATAACCCGACAAGGAACGCCA‑GCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGATCCAGACGTTATTGcc  >  2:1349166/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CGCTTTGTTCATGCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCA‑GCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGATCCAGACGTTATTGCC  >  NC_000913/361164‑361458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑