Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913361,3200CA24.9% 575.9 / 42.2 245V181F (GTT→TTT) lacAgalactoside O‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base C (64/120);  new base A (61/0);  total (125/120)
Fisher's exact test for biased strand distribution p-value = 9.91e-23
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 253 total aligned reads displayed.

TCATGCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGATCCAGACGTTATTGCCAATCGTTATC  >  NC_000913/361172‑361468
                                                                                                                                                    |                                                                                                                                                    
tCATGCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAAc                                                                                                                                                      <  1:2523814/149‑1 (MQ=255)
 cATGCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAAcc                                                                                                                                                     <  2:335019/149‑1 (MQ=255)
      cGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACa                                                                                                                                                <  1:2890929/149‑1 (MQ=255)
      cGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACa                                                                                                                                                <  1:605555/149‑1 (MQ=255)
        gATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTGTTTTTAGCGTATAAACCGACAAg                                                                                                                                              >  1:1590983/1‑149 (MQ=39)
             ggCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACg                                                                                                                                         <  2:2773175/149‑1 (MQ=255)
              gCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTTTTTAGCGAATAACCCCACAAAGAACGc                                                                                                                                        >  2:218941/1‑149 (MQ=255)
              gCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGCGAATAACCCCACAAGGAACGc                                                                                                                                        >  1:1890707/1‑149 (MQ=255)
                 aaTGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTTTTTAGCGAATAAACCCACAAGGAACGcccg                                                                                                                                     >  1:747533/1‑147 (MQ=255)
                   tGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTGTATTTATCGAAAAAACCCACAACGAACCCCCgcc                                                                                                                                   >  1:2047248/1‑149 (MQ=38)
                        aTCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGCGAAAAAACCGACCAGGAACCCCAGCCGCCac                                                                                                                              >  1:2014174/1‑149 (MQ=39)
                         tCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCAAATAACCCGACAAGGAACGCCAGCCGCCacg                                                                                                                             >  2:1786436/1‑149 (MQ=255)
                         tCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGCGAATAACCCGACAACGAACGCCAGTCGCCacg                                                                                                                             >  2:1585626/1‑149 (MQ=255)
                         tCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGCGAATAAACCGACAAGGAACCCCCGCCCCCacc                                                                                                                             >  1:2509004/1‑148 (MQ=39)
                          cGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGCGAATAAACCCACAAGGAACGCCAGCCGCCacca                                                                                                                            >  2:764025/1‑147 (MQ=255)
                             tGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCacgacgg                                                                                                                         >  2:1982524/1‑148 (MQ=255)
                             tGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGt                                                                                                                         <  1:135406/149‑1 (MQ=255)
                             tGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGt                                                                                                                         <  1:884326/149‑1 (MQ=255)
                              gAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGtt                                                                                                                        <  2:857747/149‑1 (MQ=255)
                              gAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAAAAACCCGACAAGGAACGCCAGCCGCCACGACGtt                                                                                                                        >  2:167332/1‑149 (MQ=255)
                               aaCTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGttt                                                                                                                       <  1:1743257/149‑1 (MQ=255)
                                aCTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTtg                                                                                                                      <  2:2709864/149‑1 (MQ=255)
                                aCTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTtg                                                                                                                      >  2:1541684/1‑149 (MQ=255)
                                 cTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTtgg                                                                                                                     <  1:1636685/149‑1 (MQ=255)
                                   tGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGGATTACCCGACAAGGAACGCCAGCCGCCccgacgggtgggg                                                                                                                   >  1:239207/1‑142 (MQ=255)
                                   tGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTGTATTTAGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGGTtggtg                                                                                                                   >  1:1427377/1‑149 (MQ=255)
                                     tAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTTTTTGGCGAAAAAACCCACCAGGAACCCCAGCCCCCACCACCTTttgtggg                                                                                                                 >  1:1263784/1‑148 (MQ=255)
                                     tAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGa                                                                                                                 <  2:1049733/149‑1 (MQ=255)
                                      aGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAAGAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGaa                                                                                                                <  2:1893589/149‑1 (MQ=255)
                                         ccTGATACGCTGCGCTTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTTTTTAGGGGATAAACCCACAAAGAACGACAGCCCCCACGAACTTTTGTTGAAttt                                                                                                             >  1:1112839/1‑147 (MQ=255)
                                         ccTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCCGCCGCCACGACGGTTGGTGGAATGt                                                                                                             >  2:2387868/1‑149 (MQ=255)
                                         ccTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAAACCGACAAGGAACGCCAGCCGCCACGACGGTTGGTGGAATGt                                                                                                             >  2:189800/1‑149 (MQ=255)
                                         ccTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTGTTTTTAGCGTATAACCCGCCCAGGAACGCCAGCCCCCCCGACGTGTGGGGGGATGt                                                                                                             >  1:1119471/1‑149 (MQ=255)
                                            gATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCtt                                                                                                          <  2:1398459/149‑1 (MQ=255)
                                            gATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCtt                                                                                                          <  1:1804059/149‑1 (MQ=255)
                                            gATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCtt                                                                                                          <  1:711716/149‑1 (MQ=255)
                                            gATAAGCGCAGAGTAACACGCAATTTTTATAATTTAAACTGACTATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCtt                                                                                                          <  2:2680199/149‑1 (MQ=255)
                                             aTAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTTTTTAGCGAATAAACCCACAAAGCACCCCACCCGCCCCCACGGGTTGTGGGATGtggtt                                                                                                         >  1:37356/1‑145 (MQ=255)
                                             aTAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCttt                                                                                                         <  2:31468/149‑1 (MQ=255)
                                             aTAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCttt                                                                                                         <  2:437050/149‑1 (MQ=255)
                                              tAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCtttt                                                                                                        <  1:2981383/149‑1 (MQ=255)
                                               aaGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTtg                                                                                                       <  2:2112313/149‑1 (MQ=255)
                                                aGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTGTATTTGGCGAATAAACCGACAAGGAAAGCCACCCGCCACGACGTTTGGTGGAATGTCTTTtgt                                                                                                      >  2:1285914/1‑149 (MQ=255)
                                                 gcgcAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTtgtg                                                                                                     <  2:2262638/149‑1 (MQ=255)
                                                  cgcAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGa                                                                                                    <  1:3048452/149‑1 (MQ=255)
                                                  cgcAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGGTTGGTGGTATGTCTTTTGTGa                                                                                                    >  2:1360339/1‑149 (MQ=255)
                                                      gCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTTTTTAGCGAAAAAACCCACAAAGAAACCCAGCCCCCACGACGTTTGGTGGAATTTGTTTTGTTTCGAt                                                                                                >  1:344677/1‑149 (MQ=255)
                                                              ggCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGCGAATAAACCCACAACGAACCCCAGCCCCCACGACGGTTGGTGGTATGTCTTTTTTGACGATACTAaccc                                                                                        >  1:2671377/1‑148 (MQ=255)
                                                                cAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCagcg                                                                                      <  2:2780979/149‑1 (MQ=255)
                                                                cAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTGTTTTTAGCGAATAAACCCACAACGAACCCCCGCCGCCACCACCGTTGGTGGAATGTATTTTGTGAAGATAATAaccccc                                                                                      >  1:1738642/1‑146 (MQ=255)
                                                                      ttATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATa                                                                                <  1:2434441/149‑1 (MQ=255)
                                                                       tataATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGCGAATAACCCGACAAGGAAAGCCAGCCGCCACGACGTTTGGTGGAATGTATTTTGTGACGATTCTAACCCCGCCAaaaa                                                                               >  2:528791/1‑149 (MQ=255)
                                                                        ataATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAAc                                                                              <  1:2617039/149‑1 (MQ=255)
                                                                         taATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTTTTTGGCGAAAAAACCCCCAAGGCACGCCAGCCGCCCCCACGGTTTGTGGGATGTAGTTTTTTTCGATACTAACCCCGCCCAAAACa                                                                             >  1:1243114/1‑149 (MQ=255)
                                                                             ttAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTTTTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAAt                                                                         >  2:2326474/1‑149 (MQ=255)
                                                                             ttAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAAt                                                                         <  2:300830/149‑1 (MQ=255)
                                                                                   tGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAAACCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTGTTTTTTGACGATAATACCCGCGCCAATAACAGAAATATacc                                                                   >  1:2231197/1‑149 (MQ=255)
                                                                                    gACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGCGAATAAACCGACAAGGAAAGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTAACCCCGCCCATAACAGAATTATaccc                                                                  >  2:970174/1‑149 (MQ=255)
                                                                                       gATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGAt                                                               <  1:1982524/149‑1 (MQ=255)
                                                                                         ttCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATgg                                                             <  2:2879800/149‑1 (MQ=255)
                                                                                         ttCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGCCAAAAACCCCACAAAGAACGCCAGCCGCCACGACGTTTTGTGGAATTTATTTTGTGACGATACTAACCGCGCCCATAACAGAATTATACCCGATgg                                                             >  2:676910/1‑149 (MQ=255)
                                                                                          tCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAAAAAACCCACAAGGAACGCCAGCCCCCACCACCTTTGGTGGAATGTCTTTTGTGACGATACAACCCCCGCCAAAAACAGAATTATACCCGATGGt                                                            >  2:708907/1‑149 (MQ=255)
                                                                                            aaCTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGa                                                          <  2:166703/149‑1 (MQ=255)
                                                                                             aCTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTTTTTAGCGAATAACCCCACAACGAACCCCAGCCGCCACCACGGTTGTTGGAATGTCTTTTGTTAAGATACTACCCCCGCCAAAAACAAAAATATACCCGATGGTTAc                                                         >  2:1770497/1‑149 (MQ=255)
                                                                                                ttATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGcc                                                      <  1:952325/149‑1 (MQ=255)
                                                                                                ttATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGcc                                                      <  1:2573184/149‑1 (MQ=255)
                                                                                                   taATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTgg                                                   <  2:1718724/149‑1 (MQ=255)
                                                                                                     aTCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGAt                                                 <  2:2291244/149‑1 (MQ=255)
                                                                                                           gAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATtaataa                                           <  2:1678557/149‑1 (MQ=255)
                                                                                                           gAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATtaataa                                           <  1:1296282/149‑1 (MQ=255)
                                                                                                            aaaTAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAAc                                          <  1:1178546/149‑1 (MQ=255)
                                                                                                            aaaTAATAGTGCTTATCCCGGTCGTTTATTTAGCGAAAAACCCGACAAGGAACGCCAGCCGCCCCGACGTTTGGGGGAAAGTCTTTTGTGACGCTACTACCCGCGCCAATAAAAGAATTATACCCGATGGTGTCGCCTGGATTAATAAc                                          >  2:2515352/1‑149 (MQ=255)
                                                                                                             aataatAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAAcc                                         <  1:2137697/149‑1 (MQ=255)
                                                                                                             aataatAGTGCTTATCCCGGTCGTTTATTTCGCGAAAAAACCCCCCAGGAACCCCAGCCGCCCCGACGGTTGGGGGGATGTGTTTTTTGTCGATACAACCCGCGCCAATAAAAGAATTATACCCCAGGGTGTAGCCGGGGTtaataaac                                         >  2:1235987/1‑147 (MQ=255)
                                                                                                                aataGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACcaca                                      <  1:574636/149‑1 (MQ=255)
                                                                                                                   aGTGCTTATCCCGGTCGTTTTTTTCGCCGCTAAACCCACAACGTACGCCAGCCGCCCCGAGGTTTGGTGGTCGGTCGTTTGTGACGTTACTAACCGCGCCAAAAAAAGAATTATCCCCGAGGGTTACGCCTGGGTTAAAAAAcacaggt                                   >  2:766436/1‑146 (MQ=255)
                                                                                                                   aGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATTTGTTTTGTGACGATACTACCCGCGCCAAAAAACGAATTATACCCCATGGTGACGCCTGGATTAAAAAACACATGa                                   >  2:1753464/1‑149 (MQ=255)
                                                                                                                   aGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGa                                   <  2:1847541/149‑1 (MQ=255)
                                                                                                                   aGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGGTTGGTGGAATGTGTTTTGTGTCGTTACTACCCGCGCCAATAACCGAAATATCCCCCCTGGTGACGCCTGGATTAATAACCACAAGa                                   >  2:3101429/1‑149 (MQ=255)
                                                                                                                      gCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACtt                                <  2:1125562/149‑1 (MQ=255)
                                                                                                                      gCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACtt                                <  1:154015/149‑1 (MQ=255)
                                                                                                                      gCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACtt                                <  2:2455811/149‑1 (MQ=255)
                                                                                                                       cTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTc                               <  2:1877843/149‑1 (MQ=255)
                                                                                                                       cTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTc                               <  1:2801102/149‑1 (MQ=255)
                                                                                                                        ttATCCCGGTCGTTTATTTAGCGAATAAACCCACAAGGAACGCCAGCCGCCACGACGGTTGGTGGAATGTCTTTTGTGACGATACTAACCGCGCCAAAAACATAATTATACCCGATGGTGTCGCCTGGATTAATAAACACATTACttac                              >  2:2162597/1‑147 (MQ=255)
                                                                                                                          aTCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATACCAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGa                            <  1:1493705/149‑1 (MQ=255)
                                                                                                                          aTCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGa                            <  1:2156036/149‑1 (MQ=255)
                                                                                                                            cccGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGATc                          <  1:522523/149‑1 (MQ=255)
                                                                                                                                  cGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGATCCAGACg                    >  2:207796/1‑149 (MQ=255)
                                                                                                                                    tttATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGATCCAGACGtt                  >  2:1465280/1‑149 (MQ=255)
                                                                                                                                     ttATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGATCCAGACGTTa                 <  2:58220/149‑1 (MQ=255)
                                                                                                                                       aTTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGATCCAGACGTTAtt               >  1:1949683/1‑149 (MQ=255)
                                                                                                                                            gcgAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGATCCAGACGTTATTGCCaa          <  2:1965815/149‑1 (MQ=255)
                                                                                                                                             cgAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGATCCAGACGTTATTGCCAAt         >  2:355493/1‑149 (MQ=255)
                                                                                                                                             cgAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGATCCAGACGTTATTGCCAAt         <  2:2047248/149‑1 (MQ=255)
                                                                                                                                              gAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGATCCAGACGTTATTGCCAATc        >  2:1563320/1‑149 (MQ=255)
                                                                                                                                               aaTAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGATCCAGACGTTATTGCCAATCg       >  1:292451/1‑149 (MQ=255)
                                                                                                                                                aTAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGATCCAGACGTTATTGCCAATCGt      <  1:1699053/149‑1 (MQ=255)
                                                                                                                                                   aCCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGATCCAGACGTTATTGCCAATCGTTAt   <  1:167332/149‑1 (MQ=255)
                                                                                                                                                    cccGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGATCCAGACGTTATTGCCAATCGTTATc  <  2:888611/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TCATGCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGATCCAGACGTTATTGCCAATCGTTATC  >  NC_000913/361172‑361468

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑