Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,639,2620TG25.4% 217.1 / 32.9 110N8H (AAC→CAC) yfgMancillary SecYEG translocon subunit
Reads supporting (aligned to +/- strand):  ref base T (21/61);  new base G (28/0);  total (49/61)
Fisher's exact test for biased strand distribution p-value = 3.56e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 120 total aligned reads displayed.

GGCAAGAGAAGCGGAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACT  >  NC_000913/2639116‑2639410
                                                                                                                                                  |                                                                                                                                                      
ggCAAGAGAAGCGGAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGtttt                                                                                                                                                      <  1:1130133/149‑1 (MQ=255)
ggCAAGAGAAGCGGAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGtttt                                                                                                                                                      <  1:553428/149‑1 (MQ=255)
ggCAAGAGAAGCGGAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGtttt                                                                                                                                                      <  1:961233/149‑1 (MQ=255)
  cAAGAGAAGCGGAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGtgttgg                                                                                                                                                    >  1:308964/1‑147 (MQ=255)
  cAAGAGAAGCGGAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGtgttag                                                                                                                                                    >  1:2531139/1‑147 (MQ=255)
   aaGAGAAGCGGAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTGGt                                                                                                                                                   >  2:615278/1‑149 (MQ=255)
      agaAGCGGAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTtct                                                                                                                                                <  1:2310297/149‑1 (MQ=255)
      agaAGCGGAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTtct                                                                                                                                                <  1:3024799/149‑1 (MQ=255)
      agaAGCGGAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTtct                                                                                                                                                <  1:2823574/149‑1 (MQ=255)
      agaAGCGGAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTtct                                                                                                                                                <  2:347266/149‑1 (MQ=255)
            ggAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTaaa                                                                                                                                         <  2:2646608/149‑1 (MQ=255)
             gAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTGGTTGT‑CGTAAAt                                                                                                                                        >  1:2101417/1‑149 (MQ=255)
              aGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTGGGTGT‑CGTAAAtt                                                                                                                                       >  1:764679/1‑149 (MQ=255)
                 gTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTcc                                                                                                                                    <  1:1330834/149‑1 (MQ=255)
                      gAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCt                                                                                                                               <  2:336490/149‑1 (MQ=255)
                       aaTCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTg                                                                                                                              <  2:727338/149‑1 (MQ=255)
                        aTCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTGT‑CGGAAATTTACACGCTGt                                                                                                                             >  2:3102970/1‑149 (MQ=255)
                        aTCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGt                                                                                                                             <  1:2297390/149‑1 (MQ=255)
                         tCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGtc                                                                                                                            <  2:2749225/149‑1 (MQ=255)
                         tCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGtc                                                                                                                            <  2:138525/149‑1 (MQ=255)
                         tCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTGGTTGTGGGTAAATTT‑CACGCTGtc                                                                                                                            >  2:29597/1‑149 (MQ=255)
                         tCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTGGTTGT‑CGTAAATTTGCACGCGGtg                                                                                                                            >  2:1306086/1‑148 (MQ=255)
                          cAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTGGTTTA‑CCTAAATTTCCACGcgggac                                                                                                                           >  2:2049759/1‑144 (MQ=255)
                          cAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTGGTTGT‑CGGAAATTTAAACGCGGtgg                                                                                                                           >  1:490229/1‑147 (MQ=255)
                          cAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTCGGTGT‑CGTAAATTTACACgaaggcc                                                                                                                           >  1:2370227/1‑143 (MQ=255)
                          cAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTAGTTCT‑CGTAAATTTACACGCTGtcc                                                                                                                           >  2:2049784/1‑149 (MQ=255)
                          cAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGGTGGTTGT‑CGTAAAATTACACGCTGtcc                                                                                                                           >  1:2370249/1‑149 (MQ=255)
                            aCCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGtcctt                                                                                                                         <  1:1111483/149‑1 (MQ=255)
                              cTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGtccttct                                                                                                                       <  2:498135/149‑1 (MQ=255)
                                 aTGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGtccttctcct                                                                                                                    <  2:2299918/149‑1 (MQ=255)
                                  tGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGtccttctcctt                                                                                                                   <  2:1508692/149‑1 (MQ=255)
                                  tGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGtccttctcctt                                                                                                                   <  2:2302712/149‑1 (MQ=255)
                                        ttCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTaa                                                                                                            <  1:2247185/149‑1 (MQ=255)
                                          ccAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAAcc                                                                                                          <  1:1222179/149‑1 (MQ=255)
                                           cAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTGTAGTTGG‑GGGAAATTTGCAAGCGGTACGTAGACTTCC‑TTAAacc                                                                                                         >  2:619025/1‑149 (MQ=255)
                                            aGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGGTCT‑CGTAAAATTACACGCTGTCCTTCTCCTTCC‑TTAAcccc                                                                                                        >  2:2583393/1‑148 (MQ=255)
                                            aGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTAGTTAT‑CGTTAATTTACACGCAGTCCGTCTCCGTCCGTTAAcca                                                                                                         >  1:1903984/1‑148 (MQ=255)
                                             gTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCATCTCCTTCC‑TTAACCCAg                                                                                                       <  1:438282/149‑1 (MQ=255)
                                              tAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTGGGTGT‑CGTAAAATTGCACGCGGTGCGTCGCCATCC‑TTAACCCCGt                                                                                                      >  1:485875/1‑149 (MQ=255)
                                                gcgcCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTaa                                                                                                    <  2:2172998/149‑1 (MQ=255)
                                                  gccagccaATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACg                                                                                                  <  1:1161444/149‑1 (MQ=255)
                                                   ccagccaATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGt                                                                                                 <  1:457360/149‑1 (MQ=255)
                                                   ccagccaATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGt                                                                                                 <  1:2005666/149‑1 (MQ=255)
                                                     agccaATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTGGTTGT‑CGGTAAATTACACCCTGTCCTTGTCCGTAC‑TTAAAACAGTAAAGTgc                                                                                               >  1:186680/1‑149 (MQ=255)
                                                      gccaATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTGGTTGT‑AGTAAAATTACAACCTGTACGTCTACTTAC‑TAAAACCAGTAAAGTgag                                                                                              >  2:3280/1‑147 (MQ=255)
                                                            cAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTAGGTGT‑AGTAAAATTACACGCTGTCCATCTCCATAC‑TTAAACCACTAAACTGCGCaaaat                                                                                        >  2:2501568/1‑147 (MQ=255)
                                                             aGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGTGCGCAAATgc                                                                                       <  2:2872297/149‑1 (MQ=255)
                                                                   ccAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGTGCGCAAATGCGCGGCt                                                                                 <  2:2277245/149‑1 (MQ=255)
                                                                    cAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGTGCGCAAATGCGCGGCTa                                                                                <  1:2676147/149‑1 (MQ=255)
                                                                     aaCGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAGCCGCTTCTACCTGGTCGTGTTGGGGGT‑CGGAAATTTACACGCGGTGCGTCTCCGTAC‑GTTAACCAGTAACGGGCGCAAATGCGCGGCGAc                                                                               >  1:1526055/1‑149 (MQ=255)
                                                                     aaCGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTCGTTGT‑CGTAAATTTACACGCTGTGCTTCTACTTGC‑TTAACCCAGTAACGTTCGCAAAAGCGCGGCAAc                                                                               >  2:2260948/1‑149 (MQ=255)
                                                                          ccAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTAt                                                                          <  2:181500/149‑1 (MQ=255)
                                                                          ccAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTAt                                                                          <  2:182450/149‑1 (MQ=255)
                                                                          ccAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTAt                                                                          <  1:577451/149‑1 (MQ=255)
                                                                           cAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGGTGT‑CGTAAATTTCCACGCTGTCCTTCTCCTTGC‑TTAACCCAGTAACGTGCGCAAATGCGCGGCTTCGCTATc                                                                         >  2:3028357/1‑149 (MQ=255)
                                                                           cAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTAGTTCT‑CGTAAATTTCCACGCGGTCCTTCTCCTTCC‑TTAACCCAGTAACGTGCGCAAAAGCGCGGCAACGCTATc                                                                         >  2:1939274/1‑149 (MQ=255)
                                                                           cAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTGGTTGT‑CCTAAATTTACACGCTGTCCGTATACTTAC‑TTAAACCACTAAAGTGCGCAAAATCGCGGCTACGCTAta                                                                         >  2:357230/1‑148 (MQ=255)
                                                                           cAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTGGTTAG‑CGTTAAATTCCACGCTGTACGTCGCCTTAC‑ATAAACCAGTAACATGCGCAAATTCGCGGCTAAGCAATc                                                                         >  2:688136/1‑149 (MQ=255)
                                                                           cAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTGGGTGT‑GGTTAATTTACACGCGGTCCTTGTCCGTCC‑TTAACCCCCTAACGTGCGCAAAAGCGCGGCTACGCGAta                                                                         >  2:296135/1‑148 (MQ=255)
                                                                           cAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTGC‑TTTACCCAGTAACCTGCGCAAATGCGCGGCTACGCGATc                                                                         >  2:2207710/1‑149 (MQ=255)
                                                                            aaaaTCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATcc                                                                        <  2:602288/149‑1 (MQ=255)
                                                                             aaaTCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTGGGTGT‑CGTAAATTTGCACGCGGGCCTTCGCCTGAC‑GTAAACCAGGAACGGGCGCAAAATCGCGGCGACGCAATACt                                                                       >  1:3022127/1‑149 (MQ=255)
                                                                              aaTCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTg                                                                      <  2:2196761/149‑1 (MQ=255)
                                                                              aaTCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTg                                                                      <  2:2196872/149‑1 (MQ=255)
                                                                                   ccccAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCaa                                                                 <  1:615278/149‑1 (MQ=255)
                                                                                      cAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTGGTTGT‑CGTAAATTTACACGCGGTCCTTATCCTTCC‑TTAACCCAGTAACGTGCGCAAAAGCGCGGCTACGCTATACAGCGCAAcag                                                              >  2:726438/1‑147 (MQ=255)
                                                                                       aaCAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTGGGTCT‑CGTAAATTTACACGCTGTCCGTATCCTTCC‑TTAAACCAGTAACGTGCGCAAAATCGCGGGGACGCGATACAGCGCAACAGc                                                             >  2:2801281/1‑149 (MQ=255)
                                                                                           gCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGtt                                                         <  2:2179586/149‑1 (MQ=255)
                                                                                           gCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGtt                                                         <  2:2469270/149‑1 (MQ=255)
                                                                                            ccAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGttt                                                        <  2:406310/149‑1 (MQ=255)
                                                                                             cAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTGGTTGT‑CGTAAATTTACACGCTGTGCTTATACTTCC‑TTTAACCAGTAACGTGCGCAAATTCGCGGCTACGCTATACTGCGCCACTGCCgggtg                                                       >  2:1813228/1‑145 (MQ=255)
                                                                                              aGTTCTTTGCCAATTTCAGCAAAAAAGAGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGTGCGCAAATGCGAGGCTACGCTATCCTGCGCAACTGCCGTTTGc                                                      <  1:2778416/149‑1 (MQ=255)
                                                                                              aGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGc                                                      <  1:1215257/149‑1 (MQ=255)
                                                                                              aGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGc                                                      <  1:2778362/149‑1 (MQ=255)
                                                                                                   tttGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCAcc                                                 <  1:3123285/149‑1 (MQ=255)
                                                                                                   tttGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCAcc                                                 <  1:3124080/149‑1 (MQ=255)
                                                                                                       ccATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCagag                                             <  1:1571134/149‑1 (MQ=255)
                                                                                                         aTTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTAGTTGT‑CGGTAATTTACACGCTGTCCTTGTGCATCC‑TTAAACCACTAACGTTCGCAAATGCGCGGCTACGCTATCCAGCGCAACAGCCCTTTTGTAACCAGAgcg                                           >  2:1887330/1‑149 (MQ=255)
                                                                                                              cAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAAt                                      <  2:1855320/149‑1 (MQ=255)
                                                                                                                gCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTGGTTGT‑CGGAAATTTACACGCTGTGCGTATCCTTCC‑TTAACCCCGTAACGTGCGCAAAAGCGCGGCGTCGCTATCCAGCGCCACTGCCGGTTTGTCACCCGAGCGCCAAtac                                    >  2:829793/1‑147 (MQ=255)
                                                                                                                 cAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTGGTTCT‑CGGAAATTTACACGCTGTCCTTCTCCTTGC‑TTAACCCAGTAAAGTGGGCAAATGGGCGGCGACGCTATCCTGCGCAACTGCCGGTTGCTCACCACAGCGCCAATcat                                   >  2:2040786/1‑147 (MQ=255)
                                                                                                                 cAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCt                                   <  2:764679/149‑1 (MQ=255)
                                                                                                                   aaaaaGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTc                                 <  1:1563534/149‑1 (MQ=255)
                                                                                                                               aCCGCTTCTACCTGGTCGTGTTCGTTGT‑CGGAAATTTCCACGCTGTCCTTCTCCGTCC‑GTAAACCCGTAAACTGCGCAAAAGCGCGGCTACGCTAAACAGCGCCACTGCCGGTTTCTCACCAGAGCGCAAATACGTAACTACAGCggtg                     >  2:2068131/1‑149 (MQ=255)
                                                                                                                                  gCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGAGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCg                  <  1:2655809/149‑1 (MQ=255)
                                                                                                                                    ttCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGtt                <  2:1340845/149‑1 (MQ=255)
                                                                                                                                     tCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTa               <  1:19310/149‑1 (MQ=255)
                                                                                                                                            ggTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGTTCGCAAATTCGCGGCTACGCTATCCAGCGCAACAGCCGTTTGCTCACCAGATCGCAAATCCTTAACAACTGCTGTGCCGTTAGCCACtt        >  2:2954864/1‑149 (MQ=255)
                                                                                                                                            ggTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACtt        <  1:2260948/149‑1 (MQ=255)
                                                                                                                                             gTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTc       <  2:485875/149‑1 (MQ=255)
                                                                                                                                             gTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTc       <  2:2709318/149‑1 (MQ=255)
                                                                                                                                             gTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCACCTTAC‑ATAACCCAGTAACGTGCGCAAAAGCGCGGCAACGCGATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACAACTGCTTTGCCGTTAGCCACATc       >  1:2184408/1‑149 (MQ=255)
                                                                                                                                               cGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAg     <  1:2501568/149‑1 (MQ=255)
                                                                                                                                               cGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAg     <  2:2658339/149‑1 (MQ=255)
                                                                                                                                               cGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAg     <  2:2673664/149‑1 (MQ=255)
                                                                                                                                               cGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAg     <  2:1626302/149‑1 (MQ=255)
                                                                                                                                                gTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGa    <  1:589351/149‑1 (MQ=255)
                                                                                                                                                 ttttCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGAc   >  1:2479237/1‑149 (MQ=255)
                                                                                                                                                  tttCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACt  >  1:1810634/1‑149 (MQ=255)
                                                                                                                                                  tttCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACt  >  2:2153757/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                      
GGCAAGAGAAGCGGAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACT  >  NC_000913/2639116‑2639410

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑