Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,639,2650CG25.0% 264.7 / 24.6 115E7Q (GAA→CAA) yfgMancillary SecYEG translocon subunit
Reads supporting (aligned to +/- strand):  ref base C (17/61);  new base G (26/0);  total (54/61)
Fisher's exact test for biased strand distribution p-value = 1.87e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 115 total aligned reads displayed.

AAGAGAAGCGGAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTC  >  NC_000913/2639119‑2639411
                                                                                                                                                  |                                                                                                                                                    
aaGAGAAGCGGAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTGGt                                                                                                                                                    >  2:615278/1‑149 (MQ=255)
   agaAGCGGAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTtct                                                                                                                                                 <  1:2310297/149‑1 (MQ=255)
   agaAGCGGAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTtct                                                                                                                                                 <  1:2823574/149‑1 (MQ=255)
   agaAGCGGAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTtct                                                                                                                                                 <  1:3024799/149‑1 (MQ=255)
        cGGAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTGGTTGT‑AGTaa                                                                                                                                           >  1:2630406/1‑149 (MQ=255)
         ggAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTaaa                                                                                                                                          <  2:2646608/149‑1 (MQ=255)
          gAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTAGTGGT‑CGGAAAt                                                                                                                                         >  2:19310/1‑149 (MQ=255)
           aGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTGGGTGT‑CGTAAAtt                                                                                                                                        >  1:764679/1‑149 (MQ=255)
              gTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTcc                                                                                                                                     <  1:1330834/149‑1 (MQ=255)
                   gAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCt                                                                                                                                <  2:336490/149‑1 (MQ=255)
                    aaTCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTg                                                                                                                               <  2:727338/149‑1 (MQ=255)
                     aTCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTGT‑CGGAAATTTACACGCTGt                                                                                                                              >  2:3102970/1‑149 (MQ=255)
                     aTCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGt                                                                                                                              <  1:2297390/149‑1 (MQ=255)
                      tCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGtc                                                                                                                             <  2:138525/149‑1 (MQ=255)
                      tCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGtc                                                                                                                             <  2:2749225/149‑1 (MQ=255)
                      tCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTGGTTGTGGGTAAATTT‑CACGCTGtc                                                                                                                             >  2:29597/1‑149 (MQ=255)
                      tCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTGGTTGT‑CGTAAATTTGCACGCGGtg                                                                                                                             >  2:1306086/1‑148 (MQ=255)
                      tCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGAGTTTAACCGCTTCTACCTGGTCGTTTTAGTATT‑CGTTAAAATAAACGCTGta                                                                                                                             >  2:29458/1‑148 (MQ=255)
                       cAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTGGTTTA‑CCTAAATTTCCACGcgggac                                                                                                                            >  2:2049759/1‑144 (MQ=255)
                       cAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTGGTTGT‑CGGAAATTTAAACGCGGtgg                                                                                                                            >  1:490229/1‑147 (MQ=255)
                       cAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTCGGTGT‑CGTAAATTTACACgaaggcc                                                                                                                            >  1:2370227/1‑143 (MQ=255)
                       cAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTAGTTCT‑CGTAAATTTACACGCTGtcc                                                                                                                            >  2:2049784/1‑149 (MQ=255)
                       cAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGGTGGTTGT‑CGTAAAATTACACGCTGtcc                                                                                                                            >  1:2370249/1‑149 (MQ=255)
                         aCCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGtcctt                                                                                                                          <  1:1111483/149‑1 (MQ=255)
                          ccTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTGGTTGG‑CGTAAATTTCCACGCTGtccgtc                                                                                                                         >  1:2935555/1‑149 (MQ=255)
                          ccTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTCGTTGT‑CGGAAATTTGCACGCTGtgcgtg                                                                                                                         >  1:1250786/1‑144 (MQ=255)
                           cTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGtccttct                                                                                                                        <  2:498135/149‑1 (MQ=255)
                               tGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGtccttctcctt                                                                                                                    <  2:1508692/149‑1 (MQ=255)
                               tGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGtccttctcctt                                                                                                                    <  2:2302712/149‑1 (MQ=255)
                                     ttCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTaa                                                                                                             <  1:2247185/149‑1 (MQ=255)
                                       ccAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAAcc                                                                                                           <  1:1222179/149‑1 (MQ=255)
                                        cAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTGGTTAG‑GGTAAATTTACACGCTGTCCTTCTACTTGC‑TTAAacc                                                                                                          >  2:619537/1‑149 (MQ=255)
                                         aGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGGTCT‑CGTAAAATTACACGCTGTCCTTCTCCTTCC‑TTAAcccc                                                                                                         >  2:2583393/1‑148 (MQ=255)
                                         aGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTAGTTAT‑CGTTAATTTACACGCAGTCCGTCTCCGTCCGTTAAcca                                                                                                          >  1:1903984/1‑148 (MQ=255)
                                          gTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCATCTCCTTCC‑TTAACCCAg                                                                                                        <  1:438282/149‑1 (MQ=255)
                                           tAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTGGTTGT‑CGTAAAATTCCACGCTGTGCGTCTCCTTGC‑TTAACCCAGt                                                                                                       >  1:2179456/1‑149 (MQ=255)
                                           tAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTGGGTGT‑CGTAAAATTGCACGCGGTGCGTCGCCATCC‑TTAACCCCGt                                                                                                       >  1:485875/1‑149 (MQ=255)
                                               gccagccaATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACg                                                                                                   <  1:1161444/149‑1 (MQ=255)
                                                ccagccaATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGt                                                                                                  <  1:2005666/149‑1 (MQ=255)
                                                ccagccaATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGt                                                                                                  <  1:457360/149‑1 (MQ=255)
                                                  agccaATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTGGTTGT‑CGGTAAATTACACCCTGTCCTTGTCCGTAC‑TTAAAACAGTAAAGTgc                                                                                                >  1:186680/1‑149 (MQ=255)
                                                   gccaATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTGGTTGT‑AGTAAAATTACAACCTGTACGTCTACTTAC‑TAAAACCAGTAAAGTgag                                                                                               >  2:3280/1‑147 (MQ=255)
                                                         cAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTAGGTGT‑AGTAAAATTACACGCTGTCCATCTCCATAC‑TTAAACCACTAAACTGCGCaaaat                                                                                         >  2:2501568/1‑147 (MQ=255)
                                                          aGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGTGCGCAAATgc                                                                                        <  2:2872297/149‑1 (MQ=255)
                                                                ccAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGTGCGCAAATGCGCGGCt                                                                                  <  2:2277245/149‑1 (MQ=255)
                                                                 cAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGTGCGCAAATGCGCGGCTa                                                                                 <  1:2676147/149‑1 (MQ=255)
                                                                  aaCGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAGCCGCTTCTACCTGGTCGTGTTGGGGGT‑CGGAAATTTACACGCGGTGCGTCTCCGTAC‑GTTAACCAGTAACGGGCGCAAATGCGCGGCGAc                                                                                >  1:1526055/1‑149 (MQ=255)
                                                                  aaCGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTCGTTGT‑CGTAAATTTACACGCTGTGCTTCTACTTGC‑TTAACCCAGTAACGTTCGCAAAAGCGCGGCAAc                                                                                >  2:2260948/1‑149 (MQ=255)
                                                                       ccAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTAt                                                                           <  2:182450/149‑1 (MQ=255)
                                                                       ccAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTAt                                                                           <  2:181500/149‑1 (MQ=255)
                                                                       ccAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTAt                                                                           <  1:577451/149‑1 (MQ=255)
                                                                        cAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTGGTTCG‑CGTAAATTTACACGCGGTGCATCTCCATCC‑TTAAACCAATAACCTGCGCAAAATCGCCGCTAACCTATc                                                                          >  2:2207484/1‑149 (MQ=255)
                                                                        cAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGGTGT‑CGTAAATTTCCACGCTGTCCTTCTCCTTGC‑TTAACCCAGTAACGTGCGCAAATGCGCGGCTTCGCTATc                                                                          >  2:3028357/1‑149 (MQ=255)
                                                                        cAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTGGTTGT‑CCTAAATTTACACGCTGTCCGTATACTTAC‑TTAAACCACTAAAGTGCGCAAAATCGCGGCTACGCTAta                                                                          >  2:357230/1‑148 (MQ=255)
                                                                        cAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTGGTTAG‑CGTTAAATTCCACGCTGTACGTCGCCTTAC‑ATAAACCAGTAACATGCGCAAATTCGCGGCTAAGCAATc                                                                          >  2:688136/1‑149 (MQ=255)
                                                                        cAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTGGGTGT‑GGTTAATTTACACGCGGTCCTTGTCCGTCC‑TTAACCCCCTAACGTGCGCAAAAGCGCGGCTACGCGAta                                                                          >  2:296135/1‑148 (MQ=255)
                                                                        cAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTGC‑TTTACCCAGTAACCTGCGCAAATGCGCGGCTACGCGATc                                                                          >  2:2207710/1‑149 (MQ=255)
                                                                         aaaaTCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATcc                                                                         <  2:602288/149‑1 (MQ=255)
                                                                          aaaTCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTGGGTGT‑CGTAAATTTGCACGCGGGCCTTCGCCTGAC‑GTAAACCAGGAACGGGCGCAAAATCGCGGCGACGCAATACt                                                                        >  1:3022127/1‑149 (MQ=255)
                                                                           aaTCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTg                                                                       <  2:2196761/149‑1 (MQ=255)
                                                                           aaTCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTg                                                                       <  2:2196872/149‑1 (MQ=255)
                                                                                   cAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTGGTTGT‑CGTAAATTTACACGCGGTCCTTATCCTTCC‑TTAACCCAGTAACGTGCGCAAAAGCGCGGCTACGCTATACAGCGCAAcag                                                               >  2:726438/1‑147 (MQ=255)
                                                                                    aaCAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTGGGTCT‑CGTAAATTTACACGCTGTCCGTATCCTTCC‑TTAAACCAGTAACGTGCGCAAAATCGCGGGGACGCGATACAGCGCAACAGc                                                              >  2:2801281/1‑149 (MQ=255)
                                                                                    aaCAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTGAGTTGT‑CGTAAATTTACACGCGGTCCATATCCGTCC‑GTAAACCAGTAACGTGCGCCAAATCGCGGCTACGCTATCCAGCGCaaaagc                                                              >  2:1130836/1‑145 (MQ=255)
                                                                                        gCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGtt                                                          <  2:2179586/149‑1 (MQ=255)
                                                                                        gCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGtt                                                          <  2:2469064/149‑1 (MQ=255)
                                                                                        gCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGtt                                                          <  2:2469270/149‑1 (MQ=255)
                                                                                         ccAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGttt                                                         <  2:406310/149‑1 (MQ=255)
                                                                                          cAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTGGTTGT‑CGTAAATTTACACGCTGTGCTTATACTTCC‑TTTAACCAGTAACGTGCGCAAATTCGCGGCTACGCTATACTGCGCCACTGCCgggtg                                                        >  2:1813228/1‑145 (MQ=255)
                                                                                           aGTTCTTTGCCAATTTCAGCAAAAAAGAGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGTGCGCAAATGCGAGGCTACGCTATCCTGCGCAACTGCCGTTTGc                                                       <  1:2778416/149‑1 (MQ=255)
                                                                                           aGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGc                                                       <  1:1215257/149‑1 (MQ=255)
                                                                                              gCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTAGTTAT‑GGTAAATTTACACGCTTTACTTATACTTAC‑TTAAAACAGTAAAGTGCGCCAATTGGCGGCTAAGCTATCCTGGGCAAATGCCGttttatca                                                    >  2:1672895/1‑144 (MQ=255)
                                                                                                tttGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCAcc                                                  <  1:3124080/149‑1 (MQ=255)
                                                                                                tttGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCAcc                                                  <  1:3123285/149‑1 (MQ=255)
                                                                                                    ccATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCagag                                              <  1:1571134/149‑1 (MQ=255)
                                                                                                      aTTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTAGTTGT‑CGGTAATTTACACGCTGTCCTTGTGCATCC‑TTAAACCACTAACGTTCGCAAATGCGCGGCTACGCTATCCAGCGCAACAGCCCTTTTGTAACCAGAgcg                                            >  2:1887330/1‑149 (MQ=255)
                                                                                                           cAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAAt                                       <  2:1855320/149‑1 (MQ=255)
                                                                                                             gCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTGGTTGT‑CGGAAATTTACACGCTGTGCGTATCCTTCC‑TTAACCCCGTAACGTGCGCAAAAGCGCGGCGTCGCTATCCAGCGCCACTGCCGGTTTGTCACCCGAGCGCCAAtac                                     >  2:829793/1‑147 (MQ=255)
                                                                                                             gCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTCGGTCT‑CGTAAATTTCCACGCTGTGCTTGTCCTTCC‑TTAACCCAGTTACCTGCGCAAATTCGCGGCTACGCTATCCAGCGCCACTGCCCTTTGCTCACCAGAGCCCAAATcc                                     >  2:3118498/1‑149 (MQ=255)
                                                                                                              cAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTGGTTCT‑CGGAAATTTACACGCTGTCCTTCTCCTTGC‑TTAACCCAGTAAAGTGGGCAAATGGGCGGCGACGCTATCCTGCGCAACTGCCGGTTGCTCACCACAGCGCCAATcat                                    >  2:2040786/1‑147 (MQ=255)
                                                                                                              cAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCt                                    <  2:764679/149‑1 (MQ=255)
                                                                                                                            aCCGCTTCTACCTGGTCGTTTTAGTTAT‑AGTAAATTTACACGCTGTCCTACTCCGTAC‑ATTACCAAGAAACCTGCCCAAATTCGCGGCAAAGCTAACCTGCGCAACAACAGATTGCTAACCACTGCGCAAATACTTCACAACTTAtgtg                      >  2:2068134/1‑149 (MQ=255)
                                                                                                                            aCCGCTTCTACCTGGTCGTGTTCGTTGT‑CGGAAATTTCCACGCTGTCCTTCTCCGTCC‑GTAAACCCGTAAACTGCGCAAAAGCGCGGCTACGCTAAACAGCGCCACTGCCGGTTTCTCACCAGAGCGCAAATACGTAACTACAGCggtg                      >  2:2068131/1‑149 (MQ=255)
                                                                                                                               gCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGAGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCg                   <  1:2655809/149‑1 (MQ=255)
                                                                                                                                 ttCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGtt                 <  2:1340845/149‑1 (MQ=255)
                                                                                                                                 ttCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGtt                 <  1:1813228/149‑1 (MQ=255)
                                                                                                                                  tCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTa                <  1:19310/149‑1 (MQ=255)
                                                                                                                                         ggTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACtt         <  1:2260948/149‑1 (MQ=255)
                                                                                                                                          gTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTc        <  2:2709318/149‑1 (MQ=255)
                                                                                                                                          gTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTc        <  2:485875/149‑1 (MQ=255)
                                                                                                                                          gTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCACCTTAC‑ATAACCCAGTAACGTGCGCAAAAGCGCGGCAACGCGATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACAACTGCTTTGCCGTTAGCCACATc        >  1:2184408/1‑149 (MQ=255)
                                                                                                                                           tCGTTTTCGTTCT‑CGTAAATTTACACGCTGTCCTTCTCCTTAC‑ATAACCCAGTAACGTGCGCAAATGCGCCGCTACGCTATCCAGCGCAACTGCCGTTTTCTCACCAGAGCGCAAATCCATCACAACTGCTGTGCCCTTAGCCACATCa       >  2:465472/1‑149 (MQ=255)
                                                                                                                                            cGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAg      <  2:2658339/149‑1 (MQ=255)
                                                                                                                                            cGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAg      <  2:2673664/149‑1 (MQ=255)
                                                                                                                                            cGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAg      <  1:2501568/149‑1 (MQ=255)
                                                                                                                                             gTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGa     <  1:589351/149‑1 (MQ=255)
                                                                                                                                              ttttCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGAc    >  1:2479237/1‑149 (MQ=255)
                                                                                                                                               tttCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACt   >  2:2153757/1‑149 (MQ=255)
                                                                                                                                               tttCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACt   >  1:1810634/1‑149 (MQ=255)
                                                                                                                                                ttCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTc  >  2:157235/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
AAGAGAAGCGGAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCT‑CGTAAATTTCCACGCTGTCCTTCTCCTTCC‑TTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTC  >  NC_000913/2639119‑2639411

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑