Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,639,2690CG28.8% 229.7 / 37.8 111E5D (GAG→GACyfgMancillary SecYEG translocon subunit
Reads supporting (aligned to +/- strand):  ref base C (17/57);  new base G (30/0);  total (54/57)
Fisher's exact test for biased strand distribution p-value = 2.34e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 120 total aligned reads displayed.

AGAAGCGGAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTCACCCAG  >  NC_000913/2639122‑2639417
                                                                                                                                                   |                                                                                                                                                      
agaAGCGGAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTtct                                                                                                                                                       <  1:3024799/149‑1 (MQ=255)
agaAGCGGAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTtct                                                                                                                                                       <  1:2310297/149‑1 (MQ=255)
agaAGCGGAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTtct                                                                                                                                                       <  1:2823574/149‑1 (MQ=255)
agaAGCGGAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTtct                                                                                                                                                       <  2:347266/149‑1 (MQ=255)
     cGGAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTGGTTGTAGTaa                                                                                                                                                 >  1:2630406/1‑149 (MQ=255)
      ggAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTaaa                                                                                                                                                <  2:2646608/149‑1 (MQ=255)
       gAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTGGTTGTCGTAAAt                                                                                                                                               >  1:2101417/1‑149 (MQ=255)
       gAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTAGTGGTCGGAAAt                                                                                                                                               >  2:19310/1‑149 (MQ=255)
        aGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTGGGTGTCGTAAAtt                                                                                                                                              >  1:764679/1‑149 (MQ=255)
                gAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCt                                                                                                                                      <  2:336490/149‑1 (MQ=255)
                 aaTCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTg                                                                                                                                     <  2:727338/149‑1 (MQ=255)
                  aTCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTGTCGGAAATTTACACGCTGt                                                                                                                                    >  2:3102970/1‑149 (MQ=255)
                  aTCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGt                                                                                                                                    <  1:2297390/149‑1 (MQ=255)
                   tCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGtc                                                                                                                                   <  2:2749225/149‑1 (MQ=255)
                   tCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGtc                                                                                                                                   <  2:138525/149‑1 (MQ=255)
                   tCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTGGTTGTGGGTAAATTTCACGCTGtc                                                                                                                                   >  2:29597/1‑149 (MQ=255)
                   tCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTGGTTGTCGTAAATTTGCACGCGGtg                                                                                                                                   >  2:1306086/1‑148 (MQ=255)
                    cAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTGGTTTACCTAAATTTCCACGcgggac                                                                                                                                  >  2:2049759/1‑144 (MQ=255)
                    cAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTGGTTGTCGGAAATTTAAACGCGGtgg                                                                                                                                  >  1:490229/1‑147 (MQ=255)
                    cAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTCGGTGTCGTAAATTTACACgaaggcc                                                                                                                                  >  1:2370227/1‑143 (MQ=255)
                    cAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTAGTTCTCGTAAATTTACACGCTGtcc                                                                                                                                  >  2:2049784/1‑149 (MQ=255)
                    cAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGGTGGTTGTCGTAAAATTACACGCTGtcc                                                                                                                                  >  1:2370249/1‑149 (MQ=255)
                      aCCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGtcctt                                                                                                                                <  1:1111483/149‑1 (MQ=255)
                       ccTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTGGTTGGCGTAAATTTCCACGCTGtccgtc                                                                                                                               >  1:2935555/1‑149 (MQ=255)
                       ccTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTCGTTGTCGGAAATTTGCACGCTGtgcgtg                                                                                                                               >  1:1250786/1‑144 (MQ=255)
                        cTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGtccttct                                                                                                                              <  2:498135/149‑1 (MQ=255)
                           aTGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGtccttctcct                                                                                                                           <  2:2299918/149‑1 (MQ=255)
                            tGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGtccttctcctt                                                                                                                          <  2:1508692/149‑1 (MQ=255)
                            tGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGtccttctcctt                                                                                                                          <  2:2302712/149‑1 (MQ=255)
                                  ttCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTaa                                                                                                                   <  1:2247185/149‑1 (MQ=255)
                                    ccAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAAcc                                                                                                                 <  1:1222179/149‑1 (MQ=255)
                                     cAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTGTAGTTGGGGGAAATTTGCAAGCGGTACGTAGACTTCCTTAAacc                                                                                                                >  2:619025/1‑149 (MQ=255)
                                      aGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCa                                                                                                               <  1:408751/149‑1 (MQ=255)
                                      aGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGGTCTCGTAAAATTACACGCTGTCCTTCTCCTTCCTTAAcccc                                                                                                               >  2:2583393/1‑148 (MQ=255)
                                      aGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTAGTTATCGTTAATTTACACGCAGTCCGTCTCCGTCCGTTAAcca                                                                                                                >  1:1903984/1‑148 (MQ=255)
                                        tAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTGGGTGTCGTAAAATTGCACGCGGTGCGTCGCCATCCTTAACCCCGt                                                                                                             >  1:485875/1‑149 (MQ=255)
                                          gcgcCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTaa                                                                                                           <  2:2172998/149‑1 (MQ=255)
                                            gccagccaATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACg                                                                                                         <  1:1161444/149‑1 (MQ=255)
                                             ccagccaATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGt                                                                                                        <  1:457360/149‑1 (MQ=255)
                                             ccagccaATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGt                                                                                                        <  1:2005666/149‑1 (MQ=255)
                                               agccaATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTGGTTGTCGGTAAATTACACCCTGTCCTTGTCCGTACTTAAAACAGTAAAGTgc                                                                                                      >  1:186680/1‑149 (MQ=255)
                                                gccaATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTGGTTGTAGTAAAATTACAACCTGTACGTCTACTTACTAAAACCAGTAAAGTgag                                                                                                     >  2:3280/1‑147 (MQ=255)
                                                      cAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTAGGTGTAGTAAAATTACACGCTGTCCATCTCCATACTTAAACCACTAAACTGCGCaaaat                                                                                               >  2:2501568/1‑147 (MQ=255)
                                                       aGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATgc                                                                                              <  2:2872297/149‑1 (MQ=255)
                                                             ccAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCt                                                                                        <  2:2277245/149‑1 (MQ=255)
                                                              cAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTa                                                                                       <  1:2676147/149‑1 (MQ=255)
                                                               aaCGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAGCCGCTTCTACCTGGTCGTGTTGGGGGTCGGAAATTTACACGCGGTGCGTCTCCGTACGTTAACCAGTAACGGGCGCAAATGCGCGGCGAc                                                                                      >  1:1526055/1‑149 (MQ=255)
                                                               aaCGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTCGTTGTCGTAAATTTACACGCTGTGCTTCTACTTGCTTAACCCAGTAACGTTCGCAAAAGCGCGGCAAc                                                                                      >  2:2260948/1‑149 (MQ=255)
                                                                    ccAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTAt                                                                                 <  2:182450/149‑1 (MQ=255)
                                                                    ccAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTAt                                                                                 <  2:181500/149‑1 (MQ=255)
                                                                     cAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTGGTTCGCGTAAATTTACACGCGGTGCATCTCCATCCTTAAACCAATAACCTGCGCAAAATCGCCGCTAACCTATc                                                                                >  2:2207484/1‑149 (MQ=255)
                                                                     cAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGGTGTCGTAAATTTCCACGCTGTCCTTCTCCTTGCTTAACCCAGTAACGTGCGCAAATGCGCGGCTTCGCTATc                                                                                >  2:3028357/1‑149 (MQ=255)
                                                                     cAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTGGTTGTCCTAAATTTACACGCTGTCCGTATACTTACTTAAACCACTAAAGTGCGCAAAATCGCGGCTACGCTAta                                                                                >  2:357230/1‑148 (MQ=255)
                                                                     cAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTGGTTAGCGTTAAATTCCACGCTGTACGTCGCCTTACATAAACCAGTAACATGCGCAAATTCGCGGCTAAGCAATc                                                                                >  2:688136/1‑149 (MQ=255)
                                                                     cAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTGGGTGTGGTTAATTTACACGCGGTCCTTGTCCGTCCTTAACCCCCTAACGTGCGCAAAAGCGCGGCTACGCGAta                                                                                >  2:296135/1‑148 (MQ=255)
                                                                      aaaaTCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATcc                                                                               <  2:602288/149‑1 (MQ=255)
                                                                       aaaTCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTGGGTGTCGTAAATTTGCACGCGGGCCTTCGCCTGACGTAAACCAGGAACGGGCGCAAAATCGCGGCGACGCAATACt                                                                              >  1:3022127/1‑149 (MQ=255)
                                                                        aaTCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTg                                                                             <  2:2196761/149‑1 (MQ=255)
                                                                        aaTCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTg                                                                             <  2:2196872/149‑1 (MQ=255)
                                                                             ccccAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCaa                                                                        <  1:615278/149‑1 (MQ=255)
                                                                                cAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTGGTTGTCGTAAATTTACACGCGGTCCTTATCCTTCCTTAACCCAGTAACGTGCGCAAAAGCGCGGCTACGCTATACAGCGCAAcag                                                                     >  2:726438/1‑147 (MQ=255)
                                                                                 aaCAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTGGGTCTCGTAAATTTACACGCTGTCCGTATCCTTCCTTAAACCAGTAACGTGCGCAAAATCGCGGGGACGCGATACAGCGCAACAGc                                                                    >  2:2801281/1‑149 (MQ=255)
                                                                                 aaCAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTGAGTTGTCGTAAATTTACACGCGGTCCATATCCGTCCGTAAACCAGTAACGTGCGCCAAATCGCGGCTACGCTATCCAGCGCaaaagc                                                                    >  2:1130836/1‑145 (MQ=255)
                                                                                     gCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGtt                                                                <  2:2469064/149‑1 (MQ=255)
                                                                                     gCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGtt                                                                <  2:2179586/149‑1 (MQ=255)
                                                                                      ccAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGttt                                                               <  2:406310/149‑1 (MQ=255)
                                                                                        aGTTCTTTGCCAATTTCAGCAAAAAAGAGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGAGGCTACGCTATCCTGCGCAACTGCCGTTTGc                                                             <  1:2778416/149‑1 (MQ=255)
                                                                                        aGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGc                                                             <  1:1215257/149‑1 (MQ=255)
                                                                                        aGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGc                                                             <  1:2778362/149‑1 (MQ=255)
                                                                                           gCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTAGTTATGGTAAATTTACACGCTTTACTTATACTTACTTAAAACAGTAAAGTGCGCCAATTGGCGGCTAAGCTATCCTGGGCAAATGCCGttttatca                                                          >  2:1672895/1‑144 (MQ=255)
                                                                                             tttGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCAcc                                                        <  1:3124080/149‑1 (MQ=255)
                                                                                             tttGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCAcc                                                        <  1:3123285/149‑1 (MQ=255)
                                                                                                 ccATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCagag                                                    <  1:1571134/149‑1 (MQ=255)
                                                                                                   aTTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTAGTTGTCGGTAATTTACACGCTGTCCTTGTGCATCCTTAAACCACTAACGTTCGCAAATGCGCGGCTACGCTATCCAGCGCAACAGCCCTTTTGTAACCAGAgcg                                                  >  2:1887330/1‑149 (MQ=255)
                                                                                                        cAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAAt                                             <  2:1855320/149‑1 (MQ=255)
                                                                                                          gCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTGGTTGTCGGAAATTTACACGCTGTGCGTATCCTTCCTTAACCCCGTAACGTGCGCAAAAGCGCGGCGTCGCTATCCAGCGCCACTGCCGGTTTGTCACCCGAGCGCCAAtac                                           >  2:829793/1‑147 (MQ=255)
                                                                                                           cAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTGGTTCTCGGAAATTTACACGCTGTCCTTCTCCTTGCTTAACCCAGTAAAGTGGGCAAATGGGCGGCGACGCTATCCTGCGCAACTGCCGGTTGCTCACCACAGCGCCAATcat                                          >  2:2040786/1‑147 (MQ=255)
                                                                                                           cAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCt                                          <  2:764679/149‑1 (MQ=255)
                                                                                                             aaaaaGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTc                                        <  1:1563534/149‑1 (MQ=255)
                                                                                                                         aCCGCTTCTACCTGGTCGTTTTAGTTATAGTAAATTTACACGCTGTCCTACTCCGTACATTACCAAGAAACCTGCCCAAATTCGCGGCAAAGCTAACCTGCGCAACAACAGATTGCTAACCACTGCGCAAATACTTCACAACTTAtgtg                            >  2:2068134/1‑149 (MQ=255)
                                                                                                                         aCCGCTTCTACCTGGTCGTGTTCGTTGTCGGAAATTTCCACGCTGTCCTTCTCCGTCCGTAAACCCGTAAACTGCGCAAAAGCGCGGCTACGCTAAACAGCGCCACTGCCGGTTTCTCACCAGAGCGCAAATACGTAACTACAGCggtg                            >  2:2068131/1‑149 (MQ=255)
                                                                                                                              ttCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGtt                       <  1:1813228/149‑1 (MQ=255)
                                                                                                                               tCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTa                      <  1:19310/149‑1 (MQ=255)
                                                                                                                                      ggTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTTCGCAAATTCGCGGCTACGCTATCCAGCGCAACAGCCGTTTGCTCACCAGATCGCAAATCCTTAACAACTGCTGTGCCGTTAGCCACtt               >  2:2954864/1‑149 (MQ=255)
                                                                                                                                      ggTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACtt               <  1:2260948/149‑1 (MQ=255)
                                                                                                                                       gTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTc              <  2:2709318/149‑1 (MQ=255)
                                                                                                                                       gTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTc              <  2:485875/149‑1 (MQ=255)
                                                                                                                                       gTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCACCTTACATAACCCAGTAACGTGCGCAAAAGCGCGGCAACGCGATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACAACTGCTTTGCCGTTAGCCACATc              >  1:2184408/1‑149 (MQ=255)
                                                                                                                                         cGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAg            <  2:2673664/149‑1 (MQ=255)
                                                                                                                                         cGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAg            <  1:2501568/149‑1 (MQ=255)
                                                                                                                                         cGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAg            <  2:2658339/149‑1 (MQ=255)
                                                                                                                                         cGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAg            <  2:1626302/149‑1 (MQ=255)
                                                                                                                                          gTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGa           <  1:589351/149‑1 (MQ=255)
                                                                                                                                           ttttCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGAc          >  1:2479237/1‑149 (MQ=255)
                                                                                                                                            tttCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACt         >  1:1810634/1‑149 (MQ=255)
                                                                                                                                             ttCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTc        >  2:157235/1‑149 (MQ=255)
                                                                                                                                                  tctcGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTCACCCa   >  2:2635111/1‑149 (MQ=255)
                                                                                                                                                  tctcGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTCACCCa   <  1:574308/149‑1 (MQ=255)
                                                                                                                                                  tctcGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTCACCCa   <  1:574322/149‑1 (MQ=255)
                                                                                                                                                   ctcGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTCACCCAg  <  2:490229/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                      
AGAAGCGGAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTCACCCAG  >  NC_000913/2639122‑2639417

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: