Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,412,7790TG26.7% 305.0 / 32.8 146V131V (GTT→GTG) yjfMDUF1190 domain‑containing protein YjfM
Reads supporting (aligned to +/- strand):  ref base T (15/92);  new base G (39/0);  total (54/92)
Fisher's exact test for biased strand distribution p-value = 1.84e-23
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 154 total aligned reads displayed.

AATGCCGACATTTGCGCGCGTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAG‑CCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGT  >  NC_000913/4412633‑4412927
                                                                                                                                                  |                                                                                                                                                     
 atGCCGACATTTGCGCGCGTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCt                                                                                                                                                    <  1:793712/149‑1 (MQ=255)
  tGCCGACATTTGCGCGCGTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTg                                                                                                                                                   <  2:446993/149‑1 (MQ=255)
   gCCGACATTTGCGCGCGTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTgg                                                                                                                                                  <  2:1431850/149‑1 (MQ=255)
    ccGACATTTGCGCGCGTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTGTGGa                                                                                                                                                 >  2:1052563/1‑149 (MQ=255)
    ccGACATTTGCGCGCGTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTCTggt                                                                                                                                                 >  1:181481/1‑148 (MQ=255)
     cGACATTTGCGCGCGTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTGTGGAt                                                                                                                                                >  1:1615698/1‑149 (MQ=255)
     cGACATTTGCGCGCGTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGAt                                                                                                                                                >  2:1850227/1‑149 (MQ=255)
      gACATTTGCGCGCGTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGAtt                                                                                                                                               <  1:361193/149‑1 (MQ=255)
       aCATTTGCGCGCGTGGCTCGAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTGTGGAttt                                                                                                                                              >  2:187346/1‑149 (MQ=255)
        cATTTGCGCGCGTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTCTGGAtttt                                                                                                                                             >  2:323786/1‑149 (MQ=255)
        cATTTGCGCGCGTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTATGGCttat                                                                                                                                             >  2:360440/1‑147 (MQ=255)
         aTTTGCGCGCGTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCGGGAttttt                                                                                                                                            >  2:2359020/1‑149 (MQ=255)
          tttGCGCGCGTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTg                                                                                                                                           <  1:493804/149‑1 (MQ=255)
          tttGCGCGCGTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTg                                                                                                                                           <  2:2099231/149‑1 (MQ=255)
           ttGCGCGCGTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGt                                                                                                                                          <  2:1470320/149‑1 (MQ=255)
                  cgTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAG‑CCg                                                                                                                                  <  1:1172164/149‑1 (MQ=255)
                       cTGGAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAG‑CCGGGTTa                                                                                                                             <  2:1857847/149‑1 (MQ=255)
                       cTGGAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTGTGGTTTTTTTTTAAT‑CAGGGGTa                                                                                                                             >  1:1672250/1‑149 (MQ=255)
                         ggAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAG‑CCGGGTTAtt                                                                                                                           <  2:572327/149‑1 (MQ=255)
                            acaacGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTGTGGATTTTTGTTAAGCCCGGGttttatt                                                                                                                         >  2:1159996/1‑144 (MQ=255)
                             caacGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAG‑CCGGGTTATTCGTa                                                                                                                       <  2:608485/149‑1 (MQ=255)
                              aacGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAG‑CCGGGTTATTCGTaa                                                                                                                      <  1:2559893/149‑1 (MQ=255)
                              aacGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAG‑CCGGGTTATTCGTaa                                                                                                                      <  1:2659670/149‑1 (MQ=255)
                                cGCCAAACCGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAG‑CCGGGTTATTCGTAAAg                                                                                                                    <  2:1966491/149‑1 (MQ=255)
                                cGCCAAAACGGCATTTTATGCCGATGTTCCTAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAG‑CCGGGTTATTCGTAAAg                                                                                                                    <  1:1897592/149‑1 (MQ=255)
                                  ccAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAG‑CCGGGTTATTCGTAAAGAt                                                                                                                  <  1:957438/149‑1 (MQ=255)
                                    aaaaCGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTGTGGATTTTTGTTAAT‑CCGGGGTATTCGTAAAGATcg                                                                                                                >  2:569404/1‑149 (MQ=255)
                                      aaCGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAG‑CCGGGTTATTCGTAAAGATcgcg                                                                                                              <  1:1193385/149‑1 (MQ=255)
                                         ggCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTGTGGATTTTTTTTTAT‑GCGGGGTTTTGGTTAATATAGCGATg                                                                                                           >  2:2820911/1‑149 (MQ=255)
                                          gCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAG‑CCGGGTTATTCGTAAAGATCGCGATGa                                                                                                          <  1:1915397/149‑1 (MQ=255)
                                            aTTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAG‑CCGGGTTATTCGTAAAGATCGCGATGAGc                                                                                                        <  1:2620026/149‑1 (MQ=255)
                                              tttATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTGTGGTTTTTTGTTTAT‑CCGGGTTATTGGGAAATATAGCGATGTGCCg                                                                                                      >  2:1928267/1‑149 (MQ=255)
                                                tATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAG‑CCGGGTTATTCGTAAAGATCGCGATGAGCCgtt                                                                                                    <  1:574028/149‑1 (MQ=255)
                                                tATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAG‑CCGGGTTATTCGTAAAGATCGCGATGAGCCgtt                                                                                                    <  2:2196562/149‑1 (MQ=255)
                                                tATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAG‑CCGGGTTATTCGTAAAGATCGCGATGAGCCgtt                                                                                                    <  2:2269847/149‑1 (MQ=255)
                                                tATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCATTTCTGGATTTTTGTTAAG‑CCGGGTTATTCGTAAAGATCGCGATGAGCCgtt                                                                                                    <  2:2295928/149‑1 (MQ=255)
                                                  tGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAG‑CCGGGTTATTCGTAAAGATCGCGATGAGCCgtttg                                                                                                  <  1:2204713/149‑1 (MQ=255)
                                                  tGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAG‑CCGGGTTATTCGTAAAGATCGCGATGAGCCgtttg                                                                                                  <  1:847423/149‑1 (MQ=255)
                                                  tGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAG‑CCGGGTTATTCGTAAAGATCGCGATGAGCCgtttg                                                                                                  <  2:291175/149‑1 (MQ=255)
                                                    ccGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTGTGGTTTTTTTTTAAT‑ACGGGGTATTAGGAAATATAGCGGTGTGCCgtttttt                                                                                                >  2:630673/1‑149 (MQ=255)
                                                     cGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACACTGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAG‑CCGGGTTATTCGTAAAGATCGCGATGAGCCgtttgttt                                                                                               <  2:1669953/149‑1 (MQ=255)
                                                        tGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAG‑CCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTtata                                                                                            <  1:1646958/149‑1 (MQ=255)
                                                             cGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAG‑CCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAgc                                                                                       <  2:1398841/149‑1 (MQ=255)
                                                              gaagaaTATGTCTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAG‑CCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAgcg                                                                                      <  1:560221/149‑1 (MQ=255)
                                                              gaagaaTATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAG‑CCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAgcg                                                                                      <  1:1270760/149‑1 (MQ=255)
                                                              gaagaaTATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTGTGGTTTTTTTTTAAT‑CCGGGTTATTAGTAAATATAGCGATGATCCGGTTGTTTATaaaaaag                                                                                      >  2:1405860/1‑144 (MQ=255)
                                                               aagaaTATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTGTGGATTTTTTTTAAG‑ACGGGGTTTTGGTAAATATAGCGATGAGCCGGTTTTTTATAAAAgcgg                                                                                     >  2:917591/1‑149 (MQ=255)
                                                                agaaTATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTGGGGATTTTTTTTAAT‑ACGGGGTATTCGTAAAGATAGCGATGAGCCGGTTGTTTATTAAAtcggc                                                                                    >  2:1743729/1‑149 (MQ=255)
                                                                       gACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAG‑CCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCct                                                                             <  2:1642240/149‑1 (MQ=255)
                                                                          tCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAG‑CCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCtt                                                                          <  2:2660158/149‑1 (MQ=255)
                                                                          tCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAG‑CCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCtt                                                                          <  2:2780651/149‑1 (MQ=255)
                                                                           cagcagAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAG‑CCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCttt                                                                         <  1:2709992/149‑1 (MQ=255)
                                                                           cagcagAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAG‑CCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCttt                                                                         <  2:2790285/149‑1 (MQ=255)
                                                                             gcagAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTCGTGATTTTTTTTTAT‑ACGGGGTATTTCTAAAAAAAGCGATGTTCCGTTTTTTTTTAAAAACGGCGGGTAGTCttttt                                                                       >  1:2437037/1‑148 (MQ=255)
                                                                             gcagAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGGGTGGATTTTTGTTAAT‑CCGGGGTTTTGGGAAATATAGCGATTAGCCCGTTGTTTATAACAGCGGCGGTTTCTCttttt                                                                       >  1:2436728/1‑148 (MQ=255)
                                                                              cagAACTGTCAGTATAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAG‑CCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGc                                                                      <  1:2161966/149‑1 (MQ=255)
                                                                                 aaCTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTGTGGTTTTTTTTTTAG‑GCGGGGTATTAGTAAATATAGCGATGTGCCGGTTGTTTTTAAAATCGGCGGGTACGCtttttgtta                                                                   >  1:1950887/1‑144 (MQ=255)
                                                                                  aCTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTGTGGATTTTTGTTTAT‑CCGGGGTATTGTGAAAGATCGCGATGAGCCGTTTGTTTATAAAAGCGGCGGTTGCTCTTTTTCttag                                                                  >  2:379326/1‑147 (MQ=255)
                                                                                     gTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAG‑CCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCgcgc                                                               <  1:661365/149‑1 (MQ=255)
                                                                                       cAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTTAG‑CCGGGTTTTTCGTAAAGATAGCGATGAGCCGTTTTTTTATAACATCGGCGGTTACACTTTTTCTTGGCGccc                                                             >  2:3007977/1‑149 (MQ=255)
                                                                                         gTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTGTGGATTTTTGTTAAT‑CCGGGGTATTAGTAAATATAGCGGTGTGCCGTTTTTTTTTAACATCGGCGGTTACTCGTTTTCTTAGCGccccg                                                           >  2:1744279/1‑147 (MQ=255)
                                                                                          tCTAAGTACGAAAATTGCTTCTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGGGTGGATTTTTGTTAAG‑CCGGGGTATTCGTAAAGAACGCGAAGTGCCGATTGTTTATAAAAACCGCGGTTACTCTTTTTCTTCGCGCCCAGt                                                          >  2:2939061/1‑149 (MQ=255)
                                                                                           cTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTTAT‑CCGGGTTATTAGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCACTTTTTGTTAGCGCCCCGTc                                                         >  2:2586786/1‑149 (MQ=255)
                                                                                            tAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAG‑CCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCt                                                        <  2:2117058/149‑1 (MQ=255)
                                                                                              aGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTCTGGATTTTTTTTTAG‑CCGGGGTTTTAGTAAAGATAGCGATGATCCGTTTTTTTTTAAAAGCGGCGGTTGCGCTTTTGGGTAGCGCCCCGTCggg                                                      >  2:1360645/1‑149 (MQ=255)
                                                                                                 aCGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTGTGGATTTTTTTTAAT‑CCGGGGTTTTGGTAAAGATAGCGATGTGCCGGTTGTTTATAAAAGCGGCGGGTACTCTTTTTCTTAGCGCCCCGTCTGgcgc                                                   >  2:2562223/1‑149 (MQ=255)
                                                                                                       aTTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAG‑CCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACt                                             <  1:1850227/149‑1 (MQ=255)
                                                                                                        ttGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAG‑CCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACtt                                            <  2:892200/149‑1 (MQ=255)
                                                                                                          gCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAG‑CCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCt                                          <  1:1119457/149‑1 (MQ=255)
                                                                                                          gCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAG‑CCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCt                                          <  2:2418295/149‑1 (MQ=255)
                                                                                                            tactacGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAG‑CCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTgg                                        <  2:2347336/149‑1 (MQ=255)
                                                                                                            tactacGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAG‑CCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTgg                                        <  1:500298/149‑1 (MQ=255)
                                                                                                              ctacGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAG‑CCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGCCGCAGCACTTCTGGTg                                      <  2:2727170/149‑1 (MQ=255)
                                                                                                               tacGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAG‑CCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGa                                     <  2:1582919/149‑1 (MQ=255)
                                                                                                                  gACAATGTTGAACAGAGTTGGATCCCGGTCGTGTGTGGTTTTTTGTTTAG‑CCGGGGTTTTAGTGAAGATAGCGAGGAGCCGGTTGGTTTTAAAAGCGGCGGGTGCTCGTTTTGTTAGCGCCCCCTATGGGGCCGCACAACTGGTGAttt                                  >  2:1328084/1‑148 (MQ=255)
                                                                                                                   aCAATGTTGAACAGAGTTGGATCCCGGTCGTTTGTGGATTTTTTTTTAT‑GCCGGTTGTTAGTAAATTTAGCGATGTTCCGTTTGTTTATAAAAGCGGCGGGTACTATTTTTGGTCGCGCCCCGTCTGGCGCCGCACATCTGGGGATTaa                                 >  2:219034/1‑148 (MQ=255)
                                                                                                                   aCAATGTTGAACAGAGTTGGATCCCGGTCGTGTGTGGATTTTTTTTTAG‑CCGGGGTATTAGTTAAGTTAGCGATGAGCCGTTTTTTTTTAAAAGCGGCGGTTGCACTTTTGGGTGGCGCCCCGTCTGGGGCAGCACGTATGGTGGTTAc                                 >  2:1157593/1‑149 (MQ=255)
                                                                                                                     aaTGTTGAACAGAGTTGGATCCCGGTCGTGTCTGGATTTTTTTTAAG‑CCGGGGTATTAGTAAATATCGCGATGAGCCGTTTTTTTATAACAGCGGCGGTTACTCTTTTTCTTCGCGCCCAGTCTGGCGCAGCACTTATGGTGATTACta                               >  2:2825635/1‑148 (MQ=255)
                                                                                                                        gTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAG‑CCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTg                            <  2:1659785/149‑1 (MQ=255)
                                                                                                                        gTTGAACAGAGTTGGATCCCGGTCGTGTGGGGATTTTTTTTTAG‑CCGGGTTATTCGTAAATATCGCGGTGAGCCGGTTTTTTATAAAAGCGGCGGTTGCACTTTTGGTTGGCCCCCCGTCTGGCGCAGCACGTATGGGGGTTACtacag                            >  2:408272/1‑145 (MQ=255)
                                                                                                                             aCAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAG‑CCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCt                       <  1:1466521/149‑1 (MQ=255)
                                                                                                                              cAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAG‑CCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTc                      <  1:2946709/149‑1 (MQ=255)
                                                                                                                              cAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAG‑CCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTc                      <  2:2404126/149‑1 (MQ=255)
                                                                                                                                    tGGATCCCGGTCGTTTCTGGATTTTTGTTAAG‑CCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTc                <  2:3119697/149‑1 (MQ=255)
                                                                                                                                     ggATCCCGGTCGTTTCTGGATTTTTGTTAAG‑CCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCt               <  2:1430662/149‑1 (MQ=255)
                                                                                                                                      gATCCCGGTCGTTTCTGGATTTTTGTTAAG‑CCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTg              <  1:2733189/149‑1 (MQ=255)
                                                                                                                                      gATCCCGGTCGTTTCTGGATTTTTGTTAAG‑CCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTg              <  1:2561590/149‑1 (MQ=255)
                                                                                                                                             gTCGTTTCTGGATTTTTGTTAAG‑CCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCGGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCaaaaa       >  2:3006904/1‑149 (MQ=255)
                                                                                                                                             gTCGTTTCTGGATTTTTGTTAAG‑CCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCaaaaa       <  2:1642928/149‑1 (MQ=255)
                                                                                                                                             gTCGTTTCTGGATTTTTGTTAAG‑CCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCaaaaa       <  2:2734685/149‑1 (MQ=255)
                                                                                                                                             gTCGTTTCTGGATTTTTGTTAAG‑CCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCaaaaa       <  1:306188/149‑1 (MQ=255)
                                                                                                                                               cGTTTCTGGATTTTTGTTAAG‑CCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAag     <  1:2359020/149‑1 (MQ=255)
                                                                                                                                               cGTTTCTGGATTTTTGTTAAG‑CCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAag     <  1:289987/149‑1 (MQ=255)
                                                                                                                                               cGTTTCTGGATTTTTGTTAAG‑CCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAag     <  2:464748/149‑1 (MQ=255)
                                                                                                                                               cGTTTCTGGATTTTTGTTAAG‑CCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAag     <  1:708379/149‑1 (MQ=255)
                                                                                                                                                 tttCTGGATTTTTGTTAAG‑CCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAagag   <  2:1551491/149‑1 (MQ=255)
                                                                                                                                                 tttCTGGATTTTTGTTAAG‑CCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAagag   >  1:807851/1‑149 (MQ=255)
                                                                                                                                                 tttCTGGATTTTTGTTAAG‑CCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAagag   >  2:779875/1‑149 (MQ=255)
                                                                                                                                                 tttCTGGATTTTTGTTAAG‑CCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAagag   >  1:418179/1‑149 (MQ=255)
                                                                                                                                                  ttCTGGATTTTTGTTAAG‑CCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGt  >  1:2340958/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
AATGCCGACATTTGCGCGCGTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAG‑CCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGT  >  NC_000913/4412633‑4412927

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑