| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,011,323 | +T | intergenic (‑258/+192) | PP_0871 ← / ← SBRG_Ecoli_galM | glycine betaine/carnitine/choline ABC transporter permease/SBRG_Ecoli_galM |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,011,323 | 1 | . | T | 97.4% | 126.4 / ‑3.0 | 39 | intergenic (‑258/+192) | PP_0871/SBRG_Ecoli_galM | glycine betaine/carnitine/choline ABC transporter permease/SBRG_Ecoli_galM |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base T (17/21); total (18/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.62e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01 | |||||||||||
GCTGATGAAACCAGTCCCCAACCGAAGCGGGTTGCAGGGGAGTGGACGTCTCCGTGGGCGTAAAGGTTCCCATCTGAGGCGGCATTTGGCCAGTGTTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGAT‑G‑CTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCAACAGCAATGCTTACGCATAACCATAGCGAAAATAGTGGCGCAGTGTAAGGTTGTTGTGAATATTGAGTTGCAAATATGTCG > NC_002947/1011180‑1011457 | gCTGATGAAACCAGTCCCCAACCGAAGCGGGTTGCAGGGGAGTGGACGTCTCCGTGGGCGTAAAGGTTCCCATCTGAGGCGGCATTTGGCCAGTGTTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGAT‑GTCTCaa < 1:391291/150‑1 (MQ=255) cTGATTAAACCAGTCCCCAACCGAAGCGGGTTGCAGGGGATTGGACGTCTCCGTGGGCGTAAAGGTTCCAATCTGGGGCGGCATTTGGCCAGTGTTAATCGCTGGGTTGCGGCTTTTTGGGCGAGAAAAACAGCGACTTGAT‑GTCTCaaa < 1:85361/150‑1 (MQ=255) tgatgaAACCAGTCCCCAACCGAAGCGGGTTGCAGGGGAGTGGACGTCTCCGTGGGCGTAAAGGTTCCCATCTGAGGCGGCATTTGGCCAGTGTTAATCGCTGGGTTGCGGGTTCTTGGGCGATTAAAACAGCGACTTGAT‑GTCTCAAat > 2:337639/1‑150 (MQ=255) aCCAGTCCCCAACCGAAGCGGGTTGCAGGGGAGTGGACGTCTCCGTGGGCGTAAAGGTTCCCATCTGAGGCGGCATTTGGCCAGTGTTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGAT‑GTCTCAAATCATCttt > 1:543184/1‑150 (MQ=255) atctcccAACCGAAGCGGGTTGCAGGGGAGTGGACGTCTCCGTGGGCGTAAAGGTTCCCATCTGAGGCGGCATTTGGCCAGTGTTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGAT‑GTCTCAAATCATCTTTTaa < 1:1245906/146‑1 (MQ=255) cccAACCGCAGCGGGTTGCAGGGGAGTGGACGTCTCCGTGGGCGTAAAGGTTCCCATCTGAGGCGGGATTTGGCCAGTGTTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGATCT‑CTCAAATCATCTTTTAATGat > 2:582973/1‑150 (MQ=255) cGAAGCGGGTTGCAGGGGAGTGGACGTCTCCGTGGGCGTAAAGGTTCCCATCTGAGGCGGCATTTGGCCAGTGTTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGAT‑GTCTCAAATCATCTTTTAATGATaataat > 1:918561/1‑150 (MQ=255) cAGGGGAGTGGACGTCTCCGTGGGCGTAAAGGTTCCCATCTGAGGCGGCATTTGGCCAGTGTTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGAT‑GTCTCAAATCATCTTTTAATGATAATAATTCTCATTATAtt < 1:1268943/150‑1 (MQ=255) aGGGGAGTGGACGTCTCCGTGGGCGTAAAGGTTCCCATCTGAGGCGGCATTTGGCCAGTGTTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGAT‑GTCTCAAATCATCTTTTAATGATAATAATTCTCATta < 2:646218/145‑1 (MQ=255) aGGGGAGTGGACGTCTCCGTGGGCGTAAAGGTTCCCATCTGAGGCGGCATTTGGCCAGTGTTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGAT‑GTCTCAAATCATCTTTTAATGATAATAATTCTCATta > 1:646218/1‑145 (MQ=255) gggAGTGGACGTCTCCGTGGGCGTAAAGGTTCCCATCTGAGGCGGCATTTGGCCAGTGTTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGAT‑GTCTCAAATCATCTTTTAATGa < 2:187310/128‑1 (MQ=255) gggAGTGGACGTCTCCGTGGGCGTAAAGGTTCCCATCTGAGGCGGCATTTGGCCAGTGTTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGAT‑GTCTCAAATCATCTTTTAATGa > 1:187310/1‑128 (MQ=255) gAGTGGACGTCTCCGTGGGCGTAAAGGTTCCCATCTGAGGCGGCATTTGGCCAGTGTTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGAT‑GTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCcgc < 2:1279265/150‑1 (MQ=255) gTGGACGTCTCCGTGGGCGTAAAGGTTCCCATCTGAGGCGGCATTTGGCCAGTGTTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGAT‑GTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGcga < 2:918561/150‑1 (MQ=255) aCGTCTCCGTGGGCGTAAAGGTTCCCATCTGAGGCGGCATTTGGCCAGTGTTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGAT‑GTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGaa > 2:775635/1‑150 (MQ=255) tcCGTGGGCGTAAAGGTTCCCATCTGAGGCGGCATTTGGCCAGTGTTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGAT‑GTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCAAc > 1:860024/1‑150 (MQ=255) cGTGGGCGTAAAGGTTCCCATCTGAGGCGGCATTTGGCCAGTGTTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGAT‑GTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCAACAg > 2:1290941/1‑150 (MQ=255) aaaGGTTCCCATCTGAGGCGGCATTTGGCCAGTGTTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGAT‑GTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCcg < 1:1261591/127‑1 (MQ=255) aaaGGTTCCCATCTGAGGCGGCATTTGGCCAGTGTTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGAT‑GTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCcg > 2:1261591/1‑127 (MQ=255) cccATCTGAGGCGGCATTTGGCCAGTGTTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGAT‑GTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCAACAGCAATGCTTACGCATaa > 1:615323/1‑150 (MQ=255) ccATCTGAGGCGGCATTTGGCCAGTGTTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGAT‑GTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCAACAGCAATGCTTACGCATAAc > 1:490770/1‑150 (MQ=255) cTGAGGCGGCATTTGGCCAGTGTTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGAT‑GTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCAACAGCAATGCTTACGCATAACCATa < 1:775635/150‑1 (MQ=255) gAGGCGGCATTTGGCCAGTGTTAATCGCTGGGTTGCGGGATGTTGGGCGAGAAAAACAGCGACTTGAT‑GTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCAACAGCAATGCTTACGCATAACCATAGc < 1:337639/150‑1 (MQ=255) gcggcATTTGGCCAGTGTTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGAT‑GTCTCAAATCATCTTTTAATGATAATAAtt > 2:657077/1‑95 (MQ=255) gcggcATTTGGCCAGTGTTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGAT‑GTCTCAAATCATCTTTTAATGATAATAAtt < 1:657077/95‑1 (MQ=255) gcggcATTTGGCCAGTGTTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGAT‑GTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCAACAGCAATGCTTACGCATAACCATAGCGaa > 2:1175530/1‑150 (MQ=255) ggcATTTGGCCAGTGTTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGAT‑GTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCAACAGCAATGCTTACGCATAACCATAGCGaaaa < 2:543184/150‑1 (MQ=255) tttGGCCAGTGTTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGAT‑GTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCAACAGCAATGCTTACGCATAACCATAGCGAAAATAGt > 2:833825/1‑150 (MQ=255) ggCCAGTGTTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGAT‑GTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCAACAGCAATGCTTACGCATAACCATAGCGAAAATAGTGgc < 2:615323/150‑1 (MQ=255) gtgtTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGAT‑GTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCAACAGCAATGCTTACGCATAACCATAGCGAAAATAGTGGCGCAgt < 1:833825/150‑1 (MQ=255) gtgtTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGAT‑GTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCAACAGCAATGCTTACGCATAACCATAGCGAAAATAGTGGCGCAgt < 2:860024/150‑1 (MQ=255) tAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGAT‑GTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCAACAGCAATGCTTACGCATCACCATAGCGAAAATAGTGGCGCAGTGTaa < 1:881580/150‑1 (MQ=255) aaTCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGAT‑GTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCAACAGCAATGCTTACGCATAACCATAGCGAAAATAGTGGCGCAGTGTCAg < 1:1290941/150‑1 (MQ=255) ggTTTTTGGGCGAGAAAAACAGCGACTTGAT‑GTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCAACAGCAATGCTTACGCATAACCATAGCGAAAATAGTGGCGCAGTGTAAGGTTGTTGTGAATAtt > 1:738114/1‑150 (MQ=255) ttttGGGCGAGAAAAACAGCGACTTGAT‑GTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCAACAGCAATGCTTACGCATAACCATAGCGAAAATAGTGGCGCAGTGTAAGGTTGTTGTGAATATTGAg < 2:738114/150‑1 (MQ=255) ggCGAGAAAAACAGCGACTTGAT‑GTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCAACAGCAATGCTTACGCATAACCATAGCGAAAATAGTgg > 1:99034/1‑116 (MQ=255) ggCGAGAAAAACAGCGACTTGAT‑GTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCAACAGCAATGCTTACGCATAACCATAGCGAAAATAGTgg < 2:99034/116‑1 (MQ=255) gagaAAAACAGCGACTTGAT‑GTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCAACAGCAATGCTTACGCATAACCATAGCGAAAATAGTGGCGCAGTGTAAGGTTGTTCGCAATATTGAGTTGCaaaa > 1:66038/1‑149 (MQ=255) aaCAGCGACTTGAT‑GTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCAACAGCAATGCTTACGCATAACCATAGCGAAAATAGTGGCGCAGTGTAAGGTTGTTGTGAATATTGAGTTGCAAATATGTCg < 1:1175530/150‑1 (MQ=255) | GCTGATGAAACCAGTCCCCAACCGAAGCGGGTTGCAGGGGAGTGGACGTCTCCGTGGGCGTAAAGGTTCCCATCTGAGGCGGCATTTGGCCAGTGTTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGAT‑G‑CTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCAACAGCAATGCTTACGCATAACCATAGCGAAAATAGTGGCGCAGTGTAAGGTTGTTGTGAATATTGAGTTGCAAATATGTCG > NC_002947/1011180‑1011457 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |