Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 1,856,513 A→G D203G (GAT→GGT)  relA → ATP:GTP 3'‑pyrophosphotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,856,5130AG95.2% 129.2 / ‑3.3 42D203G (GAT→GGT) relAATP:GTP 3'‑pyrophosphotransferase
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (18/22);  total (19/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCGACGACGTGCGCGTGGCGCTGATCAAGCTGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCA  >  NC_002947/1856366‑1856648
                                                                                                                                                   |                                                                                                                                       
tCGACGACGTGCGCGTGGCGCTGATCAAGCTGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTc                                                                                                                                       <  1:849776/150‑1 (MQ=255)
         tGCGCGTGGCGCTGATCAAGCTGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGt                                                                                                                              <  1:122899/150‑1 (MQ=255)
                  cgcTGATCAAGCTGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTgg                                                                                                                     >  2:656516/1‑150 (MQ=255)
                   gcTGATCAAGCTGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTca                                                                                                                                      >  1:738544/1‑132 (MQ=255)
                   gcTGATCAAGCTGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTca                                                                                                                                      <  2:738544/132‑1 (MQ=255)
                      gATCAAGCTGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGt                                                                                                                              >  2:62198/1‑137 (MQ=255)
                      gATCAAGCTGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGt                                                                                                                              <  1:62198/137‑1 (MQ=255)
                      gATCAAGCTGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGa                                                                                                                 <  1:1150210/150‑1 (MQ=255)
                       aTCAAGCTGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGAc                                                                                                                <  2:30883/150‑1 (MQ=255)
                              tGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGT‑GGAGCTGGAAGACCTGTCCtt                                                                                                        >  2:7894/1‑150 (MQ=255)
                                gCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGTg                                                                                                                             <  2:1234717/128‑1 (MQ=255)
                                gCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGTg                                                                                                                             >  1:1234717/1‑128 (MQ=255)
                                gCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTAGCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTc                                                                                                       >  1:982903/1‑150 (MQ=255)
                                   gAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGc                                                                                                    >  2:1108866/1‑150 (MQ=255)
                                       gCACGTGCGCAATCCGGGCGATCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTgg                                                                                                                            <  2:167372/122‑1 (MQ=255)
                                       gCACGTGCGCAATCCGGGCGATCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTgg                                                                                                                            >  1:167372/1‑122 (MQ=255)
                                        cACGTGCGCAACACGGGCGGTAAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCt                                                                                               <  2:982903/150‑1 (MQ=255)
                                           gTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGa                                                                                            <  1:1108866/150‑1 (MQ=255)
                                             gcgcAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGc                                                                                          <  1:903961/150‑1 (MQ=255)
                                               gcAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCg                                                                                                     >  2:740381/1‑137 (MQ=255)
                                               gcAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCg                                                                                                     <  1:740381/137‑1 (MQ=255)
                                                 aaTCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGa                                                                                      <  1:503357/150‑1 (MQ=255)
                                                            tCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTc                                                                                                                                       <  1:1230198/90‑1 (MQ=255)
                                                              aaGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGt                                                                                                            >  2:1295747/1‑115 (MQ=255)
                                                              aaGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGt                                                                                                            <  1:1295747/115‑1 (MQ=255)
                                                                      cgATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCaa                                                                 >  1:905612/1‑150 (MQ=255)
                                                                             gagaAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAATACCTGTCCTTCCGCTACCTGGAGCCCGACCAGAACAAGCAGATCGCCAAGCTGTTg                                                          >  1:415219/1‑150 (MQ=255)
                                                                             gagaAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTg                                                          >  2:1141114/1‑150 (MQ=255)
                                                                                aaaCGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTGCAt                                                       <  1:1141114/150‑1 (MQ=255)
                                                                                                        gtgtTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTcc                                                                                                          >  2:793415/1‑75 (MQ=255)
                                                                                                        gtgtTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTcc                                                                                                          <  1:793415/75‑1 (MQ=255)
                                                                                                           ttCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTtc                            <  1:656516/150‑1 (MQ=255)
                                                                                                               aCATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTtcatca                        <  1:719502/150‑1 (MQ=255)
                                                                                                                    tACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGAc                   >  1:842340/1‑150 (MQ=255)
                                                                                                                        cgccgcTCGCGCACCGCCTGGGTATCGGTCACATCAAGT‑GGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGtgat              <  1:7894/150‑1 (MQ=255)
                                                                                                                            gcTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGAGCTTCATAAGCGTCGTAATGaa           >  2:596549/1‑150 (MQ=255)
                                                                                                                                gcgcACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAg       >  2:228762/1‑150 (MQ=255)
                                                                                                                                     ccGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCa  <  1:596549/150‑1 (MQ=255)
                                                                                                                                      cGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTAc                                                                               >  2:647970/1‑72 (MQ=255)
                                                                                                                                      cGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTAc                                                                               <  1:647970/72‑1 (MQ=255)
                                                                                                                                          tGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGAc                                                                                     <  1:1233466/62‑1 (MQ=255)
                                                                                                                                          tGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGAc                                                                                     >  2:1233466/1‑62 (MQ=255)
                                                                                                                                                   |                                                                                                                                       
TCGACGACGTGCGCGTGGCGCTGATCAAGCTGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCA  >  NC_002947/1856366‑1856648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑