Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,018,1930GC34.6% 225.7 / 175.6 162intergenic (+69/+62)prfC/potF‑Ipeptide chain release factor 3/putrescine‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (59/47);  new base C (27/29);  total (86/76)
Fisher's exact test for biased strand distribution p-value = 4.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.57e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 168 total aligned reads displayed.

GCGCCGACCCGGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAGCTTGCCC  >  NC_002947/1018044‑1018342
                                                                                                                                                     |                                                                                                                                                      
gcgcCGACCCGGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTg                                                                                                                                                        <  1:2344255/150‑1 (MQ=255)
 cgcCGACCCGGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTgg                                                                                                                                                       <  1:196503/150‑1 (MQ=255)
 cgcCGACCCGGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTgg                                                                                                                                                       <  2:1785806/150‑1 (MQ=255)
      aCCCGGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTc                                                                                                                                                  <  1:1558272/150‑1 (MQ=255)
        ccGGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCCCGCCCGCGGCCCCAGGGAGGACCACACCGGGAGGGCag                                                                                                                                                >  1:4856722/1‑149 (MQ=255)
         cGGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTATCACATGACCTCCAGGTctgg                                                                                                                                               <  2:847941/150‑4 (MQ=37)
          gggTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGATTCACCAGACCTGGAGGTCAtgt                                                                                                                                              <  2:4956949/150‑1 (MQ=255)
          gggTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCAtgt                                                                                                                                              <  1:5182687/150‑1 (MQ=255)
          gggTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCAtgt                                                                                                                                              <  2:3974005/150‑1 (MQ=255)
             tCAACCTGGCGCTGATGGACGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCACTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGGCCGGGAGGTCATGGGAt                                                                                                                                           >  1:3845186/1‑150 (MQ=39)
               aaCCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTATCACATGACCTCCAGGTCTGGTGAttc                                                                                                                                         <  2:4415162/150‑3 (MQ=25)
               aaCCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAAACACCAGACCAGGAGGTCAAGTGATAc                                                                                                                                         >  1:1608772/1‑150 (MQ=255)
                tccTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTATCACATGACCTCCAGGTCTGGTGATTcc                                                                                                                                        <  1:4452446/149‑1 (MQ=25)
                 ccTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCGGCCCTTCGCGGGCACGCCCGCTCCCACAGGTATCACATGACCTCCAGGTCTGGTGATTCCt                                                                                                                                       <  2:2480263/150‑1 (MQ=21)
                 ccTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTATCACATGACCTCCAGGTCTGGTGATTCCt                                                                                                                                       <  2:4591801/150‑1 (MQ=25)
                 ccTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTATCACATGACCTCCAGGTCTGGTGATTCCt                                                                                                                                       <  2:4460618/150‑1 (MQ=25)
                 ccTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCt                                                                                                                                       <  1:1130261/150‑1 (MQ=255)
                  cTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTATCACATGACCTCCAGGTCTGGTGATTCCtg                                                                                                                                      <  1:955388/150‑1 (MQ=25)
                  cTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTATCACATGACCTCCAGGTCTGGTGATTCCta                                                                                                                                      <  1:861664/150‑2 (MQ=25)
                  cTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCCGACCAGGAGGTCATGTGATAccgg                                                                                                                                      >  2:4078556/1‑148 (MQ=255)
                   tGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTATCACATGACCTCCAGGTCTGGTGATTcc                                                                                                                                        <  2:549955/147‑1 (MQ=25)
                   tGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTATCACATGACCTCCAGGTCTGGTGATTCCtgt                                                                                                                                     <  2:4020831/150‑1 (MQ=25)
                   tGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTATCACATGACCTCCAGGTCTGGTGATTCCtgt                                                                                                                                     <  2:1389111/150‑1 (MQ=25)
                   tGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCtgt                                                                                                                                     <  1:4572568/150‑1 (MQ=255)
                    ggCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCtgtg                                                                                                                                    <  2:597116/150‑1 (MQ=255)
                    ggCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCtgtg                                                                                                                                    <  1:4143213/150‑1 (MQ=255)
                    ggCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCtgtg                                                                                                                                    <  1:5155051/150‑1 (MQ=255)
                    ggCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTCTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCtgtg                                                                                                                                    <  1:2370015/150‑1 (MQ=255)
                     gcgcTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTATCACATGACCTCCAGGTCTGGTGATTCCTGTgg                                                                                                                                   <  2:2375376/150‑1 (MQ=25)
                     gcgcTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTATCACATGACCTCCAGGTCTGGTGATTCCTGTgg                                                                                                                                   <  1:3048538/150‑1 (MQ=25)
                     gcgcTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATTCCTGTgg                                                                                                                                   <  1:1080787/150‑1 (MQ=255)
                     gcgcTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTgg                                                                                                                                   <  2:1686015/150‑1 (MQ=255)
                     gcgcTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTgg                                                                                                                                   <  1:2226221/150‑1 (MQ=255)
                     gcgcTGATGGAAGAGCGCTGGCCGGACATCAAGGTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTATCACATGACCTCCAGGTCTGGTGATTCCTGTgg                                                                                                                                   <  2:5195356/150‑1 (MQ=21)
                       gcTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTATCACATGACCTCCAGGTCTGGTGATTCCTGTGGGa                                                                                                                                 <  1:302782/150‑1 (MQ=21)
                       gcTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGa                                                                                                                                 <  2:2541125/150‑1 (MQ=255)
                       gcTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGa                                                                                                                                 <  1:4416906/150‑1 (MQ=255)
                          taTGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCg                                                                                                                              <  1:1294878/149‑1 (MQ=255)
                             ggAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCCCCACGCCTGGCGGTCATGTGATACCTGGGGGTGCGGGc                                                                                                                           >  1:4696915/1‑150 (MQ=38)
                                          ccGGACATCACGTTCCGCGCTCCCCGCGAGCACCCTTACGGCCTGAACTCGGGGCGGCTTTGCAGCCCTGCGCGGGCACGCCCGCTCCCCCAGGAAGCACCCGCCCTGGTGGTCTTGTGATACCTGTGGGCGCGGGCGTGCCCGCGAAgg                                                                                                              <  2:2849165/150‑1 (MQ=25)
                                                aTCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATACACAGACCGGGAGGTCATGTGGTACATGTGGGAGCGGGCGTGCCCGCGAAGGGCCGcg                                                                                                        >  2:606061/1‑149 (MQ=37)
                                                  cAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAg                                                                                                      >  2:3713378/1‑150 (MQ=255)
                                                   aaGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAgg                                                                                                     >  2:3438851/1‑150 (MQ=255)
                                                                 ccGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCGCCCACAGGAATCAACAGACCTGGCGGTCATGTGTTACCTGTGGGGGCGGGCGGGGGCGCGCAGGGCCGCAAGGCGGCCCC‑AATTCaa                                                                                      >  1:3785431/1‑150 (MQ=37)
                                                                        cACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATCCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCgg                                                                               <  2:343468/150‑1 (MQ=255)
                                                                           cATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACACGAATCACCACACCGGGAGGGCATGTGAGACCGGGGGGAGCGGGCGTGCCCGCGCAGGGCCGCGAGGCGGCCCC‑ATTTCAATTTCCGGCtt                                                                            >  2:2764078/1‑150 (MQ=37)
                                                                            aTTACGGCCTGACATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACCGGAATCCCCAGACCTGGAGGACATGTGATACGTGTGGGCGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCttt                                                                           <  2:485025/150‑1 (MQ=37)
                                                                                   gcttaattCGGGGCTTTTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGTAATCACCAGACCTGGTGTTCATTTGATACCTGTGGTCGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTg                                                                    <  1:400986/143‑1 (MQ=37)
                                                                                   ccTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTg                                                                    <  2:4928705/150‑1 (MQ=255)
                                                                                                ctgctTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTcgcg                                                       <  2:4965086/150‑1 (MQ=255)
                                                                                                    tttGCAGCCCTTCGCGGGCACGCCCGCTCCCCCCCGAATAAACACACCGGGTGGTCATGTGTGACCTGTGGGAGCGGGCGGGCCCCCGAAGGGCCGCAAGGCGGCCCC‑AATTCAATTTCCCGCTTTTATAATGGTCCAGATTCGCGGAcc                                                   >  2:207801/1‑149 (MQ=255)
                                                                                                    tttGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGATCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACg                                                   >  2:1664613/1‑150 (MQ=255)
                                                                                                           cccTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACgg                                            <  2:3786656/150‑1 (MQ=255)
                                                                                                            ccTTCGCGGGCACGCCCGCTCCCACACGAAACACCACGCCGGGAGGTCATGTGAAACCGGTGGGAGCGGGCGTGCCCGCCCACGGCCGCGAGGCGGCCCC‑CAATAAAATTCCGGCTTTGATACTGGTCCAGATTCGCGGACGAAAACGGt                                           >  2:4576706/1‑150 (MQ=25)
                                                                                                                    ggCACGCCCGCCCCCACCCGAACCACCACACGTGTTGTTCTTTTTATACCTGGGGGCGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTc                                   <  1:2445268/150‑1 (MQ=255)
                                                                                                                     gCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTa                                                                                                       >  1:3867608/1‑82 (MQ=255)
                                                                                                                       ccgcccgccCCCCCCGCAATCACCAGACGTGTCGGTCTTGTTATACCTGTGGCCGGGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGcc                                <  1:2431449/149‑1 (MQ=255)
                                                                                                                       aCGCCCGCTCCCACAGGTATCACATGACCTCCAGGTCTGGTGATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGcc                                >  2:4735601/1‑150 (MQ=21)
                                                                                                                         gCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCgg                              >  1:4328185/1‑150 (MQ=255)
                                                                                                                         gCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCgg                              >  2:1470375/1‑150 (MQ=255)
                                                                                                                            cGCTCCCACAGGAATCACCAGACCTGGTGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCAt                           <  2:1878865/150‑1 (MQ=255)
                                                                                                                                cccACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGcc                       <  2:2151131/150‑1 (MQ=255)
                                                                                                                                 ccACAGGTATCACATGACCTCCAGGTCTGGTGATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCt                      >  1:556591/1‑150 (MQ=25)
                                                                                                                                  cacaGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTc                     >  1:1403345/1‑150 (MQ=255)
                                                                                                                                  cacaGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTc                     >  2:2712317/1‑150 (MQ=255)
                                                                                                                                  cacaGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTc                     >  1:4603970/1‑150 (MQ=255)
                                                                                                                                   acaGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTcc                    >  2:1158307/1‑150 (MQ=255)
                                                                                                                                   acaGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTcc                    >  2:1042219/1‑150 (MQ=255)
                                                                                                                                   acaGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTcc                    >  2:4378631/1‑150 (MQ=255)
                                                                                                                                   acaGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTcc                    >  1:3530182/1‑150 (MQ=255)
                                                                                                                                   acaGGAATCACCAGACCTGGAGGTCATGGGATGCCTGTGGGAGCGGGCGTGCCCGCGCAGGGCCGCTCGGCGGCCCC‑CTTTCAGTTTCCGGCTTTGGTACTGGTCCGGATTCGCGTACGAAGAGGGTCGGTCTTCGCCGGCAgcgcgtgc                    >  2:4943887/1‑146 (MQ=255)
                                                                                                                                    caGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCa                   >  1:2042689/1‑150 (MQ=255)
                                                                                                                                    caGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCa                   >  1:2042630/1‑150 (MQ=255)
                                                                                                                                     aGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAg                  >  2:918153/1‑150 (MQ=255)
                                                                                                                                       gAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCg                               <  2:1956804/135‑1 (MQ=255)
                                                                                                                                       gAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCg                               >  1:1956804/1‑135 (MQ=255)
                                                                                                                                        taTCACATGACCTCCAGGTCTGGTGATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGa                                                  >  1:597357/2‑115 (MQ=18)
                                                                                                                                        taTCACATGACCTCCAGGTCTGGTGATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAgcgc               >  2:1689510/2‑150 (MQ=25)
                                                                                                                                         aTCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGc                        >  2:3961054/1‑140 (MQ=255)
                                                                                                                                           cACCAGCCCGGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAAGACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATa            <  2:4657035/150‑1 (MQ=255)
                                                                                                                                            acatgACCTCCAGGTCTGGTGATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATAc           >  1:1776912/5‑150 (MQ=37)
                                                                                                                                            acatgACCTCCAGGTCTGGTGATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATAc           >  2:4858904/5‑150 (MQ=37)
                                                                                                                                            acatgACCTCCAGGTCTGGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATAc           >  2:4488976/5‑150 (MQ=38)
                                                                                                                                            acatgACCTCCAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATAc           >  1:3282790/5‑150 (MQ=255)
                                                                                                                                            acatgACCTCCAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATAc           >  1:3282813/5‑150 (MQ=255)
                                                                                                                                            aCCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATAc           >  1:3904525/1‑150 (MQ=255)
                                                                                                                                            aCCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATAc           >  1:40623/1‑150 (MQ=255)
                                                                                                                                             ccAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGcc                       >  2:2879708/1‑137 (MQ=255)
                                                                                                                                             ccAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACa          >  2:712858/1‑150 (MQ=255)
                                                                                                                                             catgACCTCCAGGTCTGGTGATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACa          >  1:3422838/4‑150 (MQ=37)
                                                                                                                                             catgACCTCCAGGTCTGGTGATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACa          >  1:3348311/4‑150 (MQ=37)
                                                                                                                                              atgACCTCCAGGTCTGGTGATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCTGCAAAGCAGCCCCGATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACa          >  1:2567238/3‑150 (MQ=18)
                                                                                                                                              atgACCTCCAGGTCTGGTGATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGc                                                                              <  1:2374297/79‑1 (MQ=14)
                                                                                                                                              atgACCTCCAGGTCTGGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAg         >  2:1260787/3‑150 (MQ=255)
                                                                                                                                              atgACCTCCAGGTCTGGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAg         >  1:349862/3‑150 (MQ=255)
                                                                                                                                               aGACCTGGAGGTCATGTGATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAGc        >  1:2890454/1‑150 (MQ=255)
                                                                                                                                               aGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAGc        >  2:1570428/1‑150 (MQ=255)
                                                                                                                                                 aCCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAGCtt      >  2:2344652/1‑150 (MQ=255)
                                                                                                                                                     ggAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAGCTTGccc  <  2:3048153/150‑1 (MQ=255)
                                                                                                                                                     ggAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAGCTTGccc  >  2:2427505/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
GCGCCGACCCGGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAGCTTGCCC  >  NC_002947/1018044‑1018342

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑