Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 1,099,817 T→G T400P (ACC→CCC)  rpoN ← RNA polymerase sigma‑54 factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,099,8170TG100.0% 815.2 / NA 227T400P (ACC→CCC) rpoNRNA polymerase sigma‑54 factor
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (107/120);  total (107/120)

Only 100 of 227 total aligned reads displayed.

AACCAGTTTCTTGATGATCGCGCGGATCGCCGTGGACGAGCATTCTCCGCCTTCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGTCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTGCTCGACGATCTGCGTGGCAACCTTCATCAGGGTTTCGTTACGGCTTTGCAGGCTC  >  NC_002947/1099671‑1099962
                                                                                                                                                  |                                                                                                                                                 
aaCCAGTTTCTTGATGATCGCGCGGATCGCCGTGGACGAGCATTCTCCGCCTTCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGa                                                                                                                                                <  2:1822429/150‑1 (MQ=255)
  ccAGTTTCTTGATGATCGCGCGGATCGCCGTGGACGAGCATTCTCCGCCTTCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGAtt                                                                                                                                              >  1:5125533/1‑150 (MQ=255)
   cAGTTTCTTGATGATCGCGCGGATCGCCGTGGACGAGCATTCTCCGCCTTCGGAGGTGCTGACATGGCTGGAGAAATAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTc                                                                                                                                             >  1:4160195/1‑150 (MQ=255)
   cAGTTTCTTGATGATCGCGCGGATCGCCGTGGACGAGCATTCTCCGCCTTCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTc                                                                                                                                             <  1:4963481/150‑1 (MQ=255)
     gTTTCTTGATGATCGCGCGGATCGCCGTGGACGAGCATTCTCCGCCTTCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGt                                                                                                                                           <  2:2140236/150‑1 (MQ=255)
     gTTTCTTGATGATCGCGCGGATCGCCGTGGACGAGCATTCTCCGCCTTCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGt                                                                                                                                           <  1:2584833/150‑1 (MQ=255)
        tCTTGATGATCGCGCGGATCGCCGTGGACGAGCATTCTCCGCCTTCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCa                                                                                                                                        >  2:1509896/1‑150 (MQ=255)
          ttGATGATCGCGCGGATCGCCGTGGACGAGCATTCTCCGCCTTCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATg                                                                                                                                      >  2:2997065/1‑150 (MQ=255)
           tgatgaTCGCGCGGATCGCCGTGGACGAGCATTCTCCGCCTTCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGc                                                                                                                                     >  1:4470796/1‑150 (MQ=255)
            gatgatCGCGCGGATCGCCGTGGACGAGCATTCTCCGCCTTCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGcc                                                                                                                                    >  1:2967586/1‑150 (MQ=255)
                atCGCGCGGATCGCCGTGGACGAGCATTCTCCGCCTTCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACg                                                                                                                                >  1:4875236/1‑150 (MQ=255)
                    cgcgGATCGCCGTGGACGAGCATTCTCCGCCTTCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTATATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCtt                                                                                                                            >  2:17165/1‑150 (MQ=255)
                    cgcgGATCGCCGTGGACGAGCATTCTCCGCCTTCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCtt                                                                                                                            >  1:830795/1‑150 (MQ=255)
                      cgGATCGCCGTGGACGAGCATTCTCCGCCTTCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCg                                                                                                                          <  1:2013936/150‑1 (MQ=255)
                           cGCCGTGGACGAGCATTCTCCGCCTTCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAAt                                                                                                                     <  2:2822333/150‑1 (MQ=255)
                           cGCCGTGGACGAGCATTCTCCGCCTTCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAAt                                                                                                                     <  2:2541369/150‑1 (MQ=255)
                              cGTGGACGAGCATTCTCCGCCTTCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTc                                                                                                                  <  1:2055468/150‑1 (MQ=255)
                                tGGACGAGCATTCTCCGCCTTCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCAt                                                                                                                <  2:4307322/150‑1 (MQ=255)
                                 ggACGAGCATTCTCCGCCTTCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATg                                                                                                               <  1:657453/150‑1 (MQ=255)
                                 ggACGAGCATTCTCCGCCTTCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATg                                                                                                               <  2:4175942/150‑1 (MQ=255)
                                 ggACGAGCATTCTCCGCCTTCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATg                                                                                                               <  2:3224362/150‑1 (MQ=255)
                                   aCGAGCATTCTCCGCCTTCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCa                                                                                                             <  1:384570/150‑1 (MQ=255)
                                   aCGAGCATTCTCCGCCTTCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCa                                                                                                             <  2:452456/150‑1 (MQ=255)
                                    cGAGCATTCTCCGCCTTCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAg                                                                                                            >  1:3784354/1‑150 (MQ=255)
                                     gAGCATTCTCCGCCTTCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGt                                                                                                           >  1:665747/1‑150 (MQ=255)
                                     gAGCATTCTCCGCCTTCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGt                                                                                                           >  2:1474724/1‑150 (MQ=255)
                                     gAGCATTCTCCACCTTCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGt                                                                                                           >  1:3633793/1‑150 (MQ=255)
                                        cATTCTCCGCCTTCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGACATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTAcc                                                                                                        >  2:1320535/1‑150 (MQ=255)
                                        cATTCTCCGCCTTCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTAcc                                                                                                        >  1:4790230/1‑150 (MQ=255)
                                         aTTCTCCGCCTTCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCa                                                                                                       >  1:2478942/1‑150 (MQ=255)
                                           tctcCGCCTTCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAAc                                                                                                     <  2:3238384/150‑1 (MQ=255)
                                             tcCGCCTTCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACgg                                                                                                   >  2:3825106/1‑150 (MQ=255)
                                             tcCGCCTTCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACgg                                                                                                   >  2:2861213/1‑150 (MQ=255)
                                              ccGCCTTCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGc                                                                                                  <  2:1652944/150‑1 (MQ=255)
                                               cGCCTTCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCt                                                                                                 >  2:4313686/1‑150 (MQ=255)
                                                 gcTTCGGAGGTGCTGACATGGCTGGATAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTTCATGTATTTCTGCGTGTTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTc                                                                                               <  1:5042151/149‑1 (MQ=255)
                                                    tCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATc                                                                                            <  2:1366701/150‑1 (MQ=255)
                                                    tCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATc                                                                                            <  2:3998608/150‑1 (MQ=255)
                                                     cGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCg                                                                                           >  2:1185948/1‑150 (MQ=255)
                                                         ggTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTtc                                                                                       <  2:3302247/150‑1 (MQ=255)
                                                         ggTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTtc                                                                                       >  2:805100/1‑150 (MQ=255)
                                                              tGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGc                                                                                  <  1:2902338/150‑1 (MQ=255)
                                                                  aTGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTg                                                                              <  1:3185818/150‑1 (MQ=255)
                                                                   tGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTgg                                                                             <  1:4695794/150‑1 (MQ=255)
                                                                   tGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTgg                                                                             <  1:135617/150‑1 (MQ=255)
                                                                    ggCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGccaagta                                                                            <  2:1699806/150‑6 (MQ=255)
                                                                     gCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTc                                                                           >  2:5101027/1‑150 (MQ=255)
                                                                       tGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGa                                                                         <  1:1160735/150‑1 (MQ=255)
                                                                       tGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGa                                                                         >  2:2593023/1‑150 (MQ=255)
                                                                       tGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGa                                                                         <  2:547847/150‑1 (MQ=255)
                                                                          agaAAAATTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGa                                                                      <  1:2709630/150‑1 (MQ=255)
                                                                           gaAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGaa                                                                     <  1:3235525/150‑1 (MQ=255)
                                                                               aaGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGc                                                                                                  >  2:3445113/1‑117 (MQ=255)
                                                                               aaGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCcg                                                                 <  2:508013/150‑1 (MQ=255)
                                                                               aaGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTACATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCcg                                                                 >  2:5187326/1‑150 (MQ=255)
                                                                                    tttCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTg                                                            <  2:2052717/150‑1 (MQ=255)
                                                                                      tCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAg                                                                                                            <  2:607436/100‑1 (MQ=255)
                                                                                        aGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTGc                                                        <  2:2302063/150‑1 (MQ=255)
                                                                                         gTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTGCt                                                       >  1:1828063/1‑150 (MQ=255)
                                                                                         gTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTGCt                                                       >  1:1365803/1‑150 (MQ=255)
                                                                                          ttCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTGCTc                                                      <  2:873409/150‑1 (MQ=255)
                                                                                           tCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTGCTcg                                                     >  2:375440/1‑150 (MQ=255)
                                                                                            cGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTGCTcga                                                    >  2:412290/1‑150 (MQ=255)
                                                                                             gTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTGCTcgac                                                   >  2:3664206/1‑150 (MQ=255)
                                                                                             gTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCGTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTGCTcgac                                                   >  1:4146145/1‑150 (MQ=255)
                                                                                              tAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCAGCGCTGGTGCTcgacg                                                  >  1:3405690/1‑150 (MQ=255)
                                                                                                gATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTGCTCGACGAt                                                >  1:1711794/1‑150 (MQ=255)
                                                                                                gATGCCACGCGGCGTGAGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTGCTCGACGAt                                                >  2:4897476/1‑150 (MQ=255)
                                                                                                 aTGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTGCTCGACGATc                                               <  1:4384854/150‑1 (MQ=255)
                                                                                                  tGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTGCTCGACGATCt                                              >  1:4230876/1‑150 (MQ=255)
                                                                                                    ccACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTGCTCGACGATCTGc                                            >  1:1554621/1‑150 (MQ=255)
                                                                                                      aCGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTGCTCGACGATCTGCGt                                          <  2:1054708/150‑1 (MQ=255)
                                                                                                      aCGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTGCTCGACGATCTGCGt                                          >  2:1218164/1‑150 (MQ=255)
                                                                                                      aCGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTGCTCGACGATCTGCGt                                          <  1:4007464/150‑1 (MQ=255)
                                                                                                      aCGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTGCTCGACGATCTGCGt                                          <  1:1739290/150‑1 (MQ=255)
                                                                                                       cgcgGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTGCTCGACGATCTGCGTg                                         <  2:3815175/150‑1 (MQ=255)
                                                                                                       cgcgGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTGCTCGACGATCTGCGTg                                         <  1:4110175/150‑1 (MQ=255)
                                                                                                       cgcgGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTGCTCGACGATCTGCGTg                                         <  1:3101029/150‑1 (MQ=255)
                                                                                                           gggTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTGCTCGACGATCTGCGTGGCaa                                     <  2:4965418/150‑1 (MQ=255)
                                                                                                             gtgtGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTGCTCGACGATCTGCGTGGCAAcc                                   >  2:1353112/1‑150 (MQ=255)
                                                                                                                tgCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTGCTCGACGATCTGCGTGGCAACCTtc                                <  1:1185948/150‑1 (MQ=255)
                                                                                                                  cATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTGCTCGACGATCTGCGTGGCAACCTtcat                              <  2:2113287/150‑1 (MQ=255)
                                                                                                                   aTGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTGCTCGACGATCTGCGTGGCAACCTtcatc                             >  1:1074546/1‑150 (MQ=255)
                                                                                                                    tGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTGCTCGACGATCTGCGTGGCAACCTtcatca                            >  2:1064972/1‑150 (MQ=255)
                                                                                                                    tGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTGCTCGACGATCTGCGTGGCAACCTtcatca                            >  1:4905190/1‑150 (MQ=255)
                                                                                                                    tGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTGCTCGACGATCTGCGTGGCAACCTtcatca                            >  1:3500812/1‑150 (MQ=255)
                                                                                                                    tGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTGCTCGACGATCTGCGTGGCAACCTtcatca                            >  1:2667819/1‑150 (MQ=255)
                                                                                                                      tATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGTCAATGTCATGCAGTACCAACGGATTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTTCTCGACGATCTGCGTGGCAACCTTCATCAgg                          <  1:3965245/150‑1 (MQ=255)
                                                                                                                       aTTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTGCTCGACGATCTGCGTGGCAACCTTCATCAggg                         >  2:686623/1‑150 (MQ=255)
                                                                                                                       aTTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTGCTCGACGATCTGCGTGGCAACCTTCATCAggg                         >  1:2454951/1‑150 (MQ=255)
                                                                                                                        tttCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTGCTCGACGATCTGCGTGGCAACCTTCATCAGGGt                        >  1:1509401/1‑150 (MQ=255)
                                                                                                                            tGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTGCTCGACGATCTGCGTGGCAACCTTCATCAGGGTTTCg                    >  2:4429941/1‑150 (MQ=255)
                                                                                                                            tGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTGCTCGACGATCTGCGTGGCAACCTTCATCAGGGTTTCg                    >  1:3580789/1‑150 (MQ=255)
                                                                                                                                 gagcaCCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTGCTc                                                      <  1:1439745/107‑1 (MQ=255)
                                                                                                                                       ccGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTGCTCGACGATCTGCGTGGCAACCTTCATCAGGGTTTCGTTACGGCTTTg         >  2:3390894/1‑150 (MQ=255)
                                                                                                                                        cgcgAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTGCTCGACGATCTGCGTGGCAACCTTCATCAGGGTTTCGTTACGGCTTTGc        >  2:4563086/1‑150 (MQ=255)
                                                                                                                                         gcgAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTGCTCGACGATCTGCGTGGCAACCTTCATCAGGGTTTCGTTACGGCTTTGCa       <  1:2234744/150‑1 (MQ=255)
                                                                                                                                           gAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTGCTCGACGATCTGCGTGGCAACCTTCATCAGGGTTTCGTTACGGCTTTGCAgg     >  1:2525945/1‑150 (MQ=255)
                                                                                                                                            aaaTGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTGCTCGACGATCTGCGTGGCAACCTTCATCAGGGTTTCGTTACGGCTTTGCAGGc    <  1:109923/150‑1 (MQ=255)
                                                                                                                                              aTGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATTGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTGCTCGACGATCTGCGTGGCAACCTTCATCAGGGTTTCGTTACGGCTTTGCAGGctc  <  2:830795/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
AACCAGTTTCTTGATGATCGCGCGGATCGCCGTGGACGAGCATTCTCCGCCTTCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGTCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTGCTCGACGATCTGCGTGGCAACCTTCATCAGGGTTTCGTTACGGCTTTGCAGGCTC  >  NC_002947/1099671‑1099962

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑