Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 1,099,817 T→G T400P (ACC→CCC)  rpoN ← RNA polymerase sigma‑54 factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,099,8170TG100.0% 1551.5 / NA 436T400P (ACC→CCC) rpoNRNA polymerase sigma‑54 factor
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (227/209);  total (227/209)

Only 100 of 436 total aligned reads displayed.

AACCAGTTTCTTGATGATCGCGCGGATCGCCGTGGACGAGCATTCTCCGCCTTCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGTCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTGCTCGACGATCTGCGTGGCAACCTTCATCAGGGTTTCGTTACGGCTTTGCAGGCTC  >  NC_002947/1099671‑1099962
                                                                                                                                                  |                                                                                                                                                 
aaCCAGTTTCTTGATGATCGCGCGGATCGCCGTGGACGAGCATTCTCCGCCTTCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGa                                                                                                                                                <  1:7445225/150‑1 (MQ=255)
    aGTTTCTTGATGATCGCGCGGATCGCCGTGGACGAGCATTCTCCGCCTTCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCg                                                                                                                                            >  2:4716137/1‑150 (MQ=255)
     gTTTCTTGATGATCGCGCGGATCGCCGTGGACGAGCATTCTCCGCCTTCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGt                                                                                                                                           <  2:1754497/150‑1 (MQ=255)
         cTTGATGATCGCGCGGATCGCCGTGGACGAGCATTCTCCGCCTTCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCAt                                                                                                                                       <  1:7280874/150‑1 (MQ=255)
         cTTGATGATCGCGCGGATCGCCGTGGACGAGCATTCTCCGCCTTCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCAt                                                                                                                                       <  1:1920767/150‑1 (MQ=255)
               gatCGCGCGGATCGCCGTGGACGAGCATTCTCCGCCTTCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTAc                                                                                                                                 <  1:811581/150‑1 (MQ=255)
                 tCGCGCGGATCGCCGTGGACGAGCATTCTCCGCCTTCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACgg                                                                                                                               <  1:9147374/150‑1 (MQ=255)
                   gcgcgGATCGCCGTGGACGAGCATTCTCCGCCTTCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCt                                                                                                                             >  2:9256488/1‑150 (MQ=255)
                   gcgcgGATCGCCGTGGACGAGCATTCTCCGCCTTCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCt                                                                                                                             >  2:3572406/1‑150 (MQ=255)
                     gcgGATCGCCGTGGACGAGCATTCTCCGCCTTCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTc                                                                                                                           <  2:4558969/150‑1 (MQ=255)
                      cgGATCGCCGTGGACGAGCATTCTCCGCCTTCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCg                                                                                                                          <  2:962411/150‑1 (MQ=255)
                       ggATCGCCGTGGACGAGCATTCTCCGCCTTCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCgg                                                                                                                         >  1:5487562/1‑150 (MQ=255)
                           cGCCGTGGACGAGCATTCTCCGCCTTCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAAt                                                                                                                     <  2:8572410/150‑1 (MQ=255)
                           cGCCGTGGACGAGCATTCTCCGCCTTCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAAt                                                                                                                     <  1:10299024/150‑1 (MQ=255)
                              cGTGGACGAGCATTCTCCGCCTTCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTATATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGAGCATGCCTACGGCTTCGGCAATGTc                                                                                                                  >  1:7076910/1‑150 (MQ=255)
                              cGTGGACGAGCATTCTCCGCCTTCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTc                                                                                                                  >  1:9661217/1‑150 (MQ=255)
                               gTGGACGAGCATTCTCCGCCTTCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCa                                                                                                                 <  2:4799114/150‑1 (MQ=255)
                               gTGGACGAGCATTCTCCGCCTTCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCa                                                                                                                 >  1:6648125/1‑150 (MQ=255)
                                 ggACGAGCATTCTCCGCCTTCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATg                                                                                                               <  1:9256293/150‑1 (MQ=255)
                                 ggACGAGCATTCTCCGCCTTCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATg                                                                                                               <  1:8316157/150‑1 (MQ=255)
                                  gACGAGCATTCTCCGCCTTCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGc                                                                                                              >  2:6322049/1‑150 (MQ=255)
                                   aCGAGCATTCTCCGCCTTCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCa                                                                                                             <  2:10111732/150‑1 (MQ=255)
                                   aCGAGCATTCTCCGCCTTCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCa                                                                                                             <  2:6816720/150‑1 (MQ=255)
                                     gAGCATTCTCCGCCTTCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGt                                                                                                           >  1:10147678/1‑150 (MQ=255)
                                     gAGCATTCTCCGCCTTCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGt                                                                                                           >  1:2191820/1‑150 (MQ=255)
                                       gCATTCTCCGCCTTCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTAc                                                                                                         >  2:5182551/1‑150 (MQ=255)
                                       gCATTCTCCGCCTTCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTAc                                                                                                         >  2:719643/1‑150 (MQ=255)
                                         aTTCTCCGCCTTCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCa                                                                                                       >  2:4135348/1‑150 (MQ=255)
                                           tctcCGCCTTCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAAc                                                                                                     >  1:3726265/1‑150 (MQ=255)
                                             tcCGCCTTCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGt                                                                                                                                           >  1:2424323/1‑110 (MQ=255)
                                             tcCGCCTTCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACgg                                                                                                   >  2:2473704/1‑150 (MQ=255)
                                              ccGCCTTCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCg                                                                                                                          <  2:2238019/126‑1 (MQ=255)
                                              ccGCCTTCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGc                                                                                                  <  2:429655/150‑1 (MQ=255)
                                               cGCCTTCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCt                                                                                                 <  2:9184091/150‑1 (MQ=255)
                                                 ccTTCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTc                                                                                               <  2:4996561/150‑1 (MQ=255)
                                                  cTTCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCa                                                                                              >  2:3109585/1‑150 (MQ=255)
                                                  cTTCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCCTCa                                                                                              >  2:5875039/1‑150 (MQ=255)
                                                   ttCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCAt                                                                                             <  1:4545106/150‑1 (MQ=255)
                                                         ggTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTtc                                                                                       <  2:2623710/150‑1 (MQ=255)
                                                         ggTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTtc                                                                                       <  1:4959889/150‑1 (MQ=255)
                                                            gCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTtcgtc                                                                                    >  2:5337993/1‑150 (MQ=255)
                                                              tGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGc                                                                                  >  2:9033931/1‑150 (MQ=255)
                                                              tGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGc                                                                                  >  2:6109843/1‑150 (MQ=255)
                                                              tGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGc                                                                                  >  2:7432337/1‑150 (MQ=255)
                                                               gACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGcc                                                                                 >  2:8185848/1‑150 (MQ=255)
                                                               gACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGcc                                                                                 >  2:8917004/1‑150 (MQ=255)
                                                                aCATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCg                                                                                <  1:5308997/150‑1 (MQ=255)
                                                                  aTGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTg                                                                              >  2:1925098/1‑150 (MQ=255)
                                                                   tGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTgg                                                                             >  1:10377227/1‑150 (MQ=255)
                                                                       tGGAGAAAAAGTATTTTAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGa                                                                         <  2:2053106/150‑1 (MQ=255)
                                                                       tGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGa                                                                         <  1:6342920/150‑1 (MQ=255)
                                                                        ggAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAg                                                                        <  1:5429554/150‑1 (MQ=255)
                                                                           gaAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGaa                                                                     >  2:3376427/1‑150 (MQ=255)
                                                                           gaAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGaa                                                                     <  1:1585796/150‑1 (MQ=255)
                                                                           gaAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGaa                                                                     >  1:10011800/1‑150 (MQ=255)
                                                                            aaaaaGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGcc                                                                                 <  1:4141626/137‑1 (MQ=255)
                                                                            aaaaaGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAg                                                                    >  1:9321965/1‑150 (MQ=255)
                                                                            aaaaaGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAg                                                                    >  1:6484215/1‑150 (MQ=255)
                                                                              aaaGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGcc                                                                  >  1:5247930/1‑150 (MQ=255)
                                                                                   agttCAGTTCGTAGATGCCACGCGGGGTGTGCATGAATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCt                                                             <  2:8652176/148‑1 (MQ=255)
                                                                                   aTTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCt                                                             <  2:10011800/150‑1 (MQ=255)
                                                                                    tttCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCCTGCAGTACCACCGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTg                                                            <  2:2751062/150‑1 (MQ=255)
                                                                                    tttCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTg                                                            <  2:8005514/150‑1 (MQ=255)
                                                                                         gTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTc                                                                                                                           >  2:10281221/1‑82 (MQ=255)
                                                                                          ttCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTGCtn                                                      <  1:1452689/150‑2 (MQ=255)
                                                                                          ttCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTGCTc                                                      >  1:5687257/1‑150 (MQ=255)
                                                                                                gATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGACGAGGAAGCCGCGCTGGTGCTCGACGAt                                                <  1:2279427/150‑1 (MQ=255)
                                                                                                 aTGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGaa                                                                     <  2:10390921/128‑1 (MQ=255)
                                                                                                  tGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTGCTCGACGATCt                                              >  1:8688650/1‑150 (MQ=255)
                                                                                                    ccACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTGCTCGACGATCTGc                                            <  1:5847235/150‑1 (MQ=255)
                                                                                                      aCGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTGCTCGACGATCTGCGt                                          <  1:1226149/150‑1 (MQ=255)
                                                                                                      aCGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTGCTCGACGATCTGCGt                                          <  2:8391825/150‑1 (MQ=255)
                                                                                                      aCGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCAAGGAAGCCGCGCTGGTGCTCGACGATCTGCGt                                          >  2:3916778/1‑150 (MQ=255)
                                                                                                       cgcgGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTGCTCGACGATCTGCGTg                                         <  2:7600053/150‑1 (MQ=255)
                                                                                                              tgtgCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTGCTCGACGATCTGCGTGGCAACCt                                  <  2:1504765/150‑1 (MQ=255)
                                                                                                                tgCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTGCTCGACGATCTGCGTGGCAACCTtc                                <  1:616253/150‑1 (MQ=255)
                                                                                                                tgCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTGCTCGACGATCTGCGTGGCAACCTtc                                <  1:3630561/150‑1 (MQ=255)
                                                                                                                 gCATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTGCTCGACGATCTGCGTGGCAACCTtca                               >  1:8911194/1‑150 (MQ=255)
                                                                                                                  cATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTGCTCGACGATCTGCGt                                          >  2:5440631/1‑138 (MQ=255)
                                                                                                                  cATGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTGCTCGACGATCTGCGTGGCAACCTtcat                              >  1:2788467/1‑150 (MQ=255)
                                                                                                                   aTGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTGCTCGACGATCTGCGTGGCAACCTtcatc                             >  1:2590112/1‑150 (MQ=255)
                                                                                                                   aTGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTGCTCGACGATCTGCGTGGCAACCTtcatc                             >  1:3310094/1‑150 (MQ=255)
                                                                                                                   aTGTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTGCTCGACGATCTGCGTGGCAACCTtcatc                             >  1:3482085/1‑150 (MQ=255)
                                                                                                                     gTATTTCTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTg                                                         >  1:9528464/1‑120 (MQ=255)
                                                                                                                           cTGCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTGCTCGACGATCTGCGTGGCAACCTTCATCAGGGTTTc                     <  2:1594069/150‑1 (MQ=255)
                                                                                                                             gCGTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTGCTCGACGATCTGCGTGGCAACCTTCATCAGGGTTTCGt                   >  2:1351620/1‑150 (MQ=255)
                                                                                                                               gTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTGCTCGACGATCTGCGTGGCAACCTTCATCAGGGTTTCGTTa                 >  2:7937300/1‑150 (MQ=255)
                                                                                                                               gTGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTGCTCGACGATCTGCGTGGCAACCTTCATCAGGGTTTCGTTa                 <  2:6715525/150‑1 (MQ=255)
                                                                                                                                tGGTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTGCTCGACGATCTGCGTGGCAACCTTCATCAGGGTTTCGTTAc                >  2:5749858/1‑150 (MQ=255)
                                                                                                                                  gTAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTGCTCGACGATCTGCGTGGCAACCTTCATCAGGGTTTCGTTACgg              <  1:6809104/150‑1 (MQ=255)
                                                                                                                                   tAACCCGCGAAATGGGCGATTCGTGCATTCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTGCTCGACGATCTGCGTGGCAACCTTCATCAGGGTTTCGTTACGGc             >  2:5066994/1‑150 (MQ=255)
                                                                                                                                   tAACCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTGCTCGACGATCTGCGTGGCAACCTTCATCAGGGTTTCGTTACGGc             >  2:7060129/1‑150 (MQ=255)
                                                                                                                                    aaCCCGCGAAATGGGCGATTCGTGCATGCCTACGGCTACGGCAATGTCATGCAGTACCCACGGCTTCATCGCTTCGTCGCCGGGGTCGAGGAAGCCGCGCTGGTGCTCGACGAGCTGCGTGGCACCCGTCAGCAGGGGGTCGTTCCGGcg            >  1:3073680/1‑149 (MQ=255)
                                                                                                                                       ccGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTGCTCGACGATCTGCGTGGCAACCTTCATCAGGGTTTCGTTACGGCTTTg         >  1:4384120/1‑150 (MQ=255)
                                                                                                                                       ccGCGAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTGCTCGACGATCTGCGTGGCAACCTTCATCAGGGTTTCGTTACGGCTTTg         >  1:8549656/1‑150 (MQ=255)
                                                                                                                                        cgcgAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTGCTCGACGATCTGCGTGGCAACCTTCATCAGGGTTTCGTTACGGCTTTGc        <  2:9469247/150‑1 (MQ=255)
                                                                                                                                         gcgAAATGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTGCTCGACGATCTGCGTGGCAACCTTCATCAGGGTTTCGTTACGGCTTTGCa       <  2:2412522/150‑1 (MQ=255)
                                                                                                                                            aaaTGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTGCTCGACGATCTGCGTGGCAACCTTCATCAGGGTTTCGTTACGGCTTTGCAGGc    <  1:3978111/150‑1 (MQ=255)
                                                                                                                                              ctGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTGCTCGACGATCTGCGTGGCAACCTTCATCAGGGTTTCGTTACGGCTTTGCAGGctc  <  2:1237674/149‑1 (MQ=255)
                                                                                                                                              aTGGGCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTGCTCGACGATCTGCGTGGCAACCTTCATCAGGGTTTCGTTACGGCTTTGCAGGctc  <  2:4206420/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
AACCAGTTTCTTGATGATCGCGCGGATCGCCGTGGACGAGCATTCTCCGCCTTCGGAGGTGCTGACATGGCTGGAGAAAAAGTATTTCAGTTCGTAGATGCCACGCGGGGTGTGCATGTATTTCTGCGTGGTAACCCGCGAAATGGTCGATTCGTGCATGCCTACGGCTTCGGCAATGTCATGCAGTACCAACGGCTTCATCGCTTCGTCGCCGTGGTCGAGGAAGCCGCGCTGGTGCTCGACGATCTGCGTGGCAACCTTCATCAGGGTTTCGTTACGGCTTTGCAGGCTC  >  NC_002947/1099671‑1099962

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑