Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	1,011,323	+T	intergenic (‑258/+192)	PP_0871 ← / ← SBRG_Ecoli_galM	glycine betaine/carnitine/choline ABC transporter permease/SBRG_Ecoli_galM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,011,323	1	.	T	100.0%	96.6 / NA	29	intergenic (‑258/+192)	PP_0871/SBRG_Ecoli_galM	glycine betaine/carnitine/choline ABC transporter permease/SBRG_Ecoli_galM
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (11/18);  total (11/18)

GAAAATGCTGATGAAACCAGTCCCCAACCGAAGCGGGTTGCAGGGGAGTGGACGTCTCCGTGGGCGTAAAGGTTCCCATCTGAGGCGGCATTTGGCCAGTGTTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGATG‑CTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCAACAGCAATGCTTACGCATAACCATAGCGAAAATAGTGGCGCAGTGTAAGGTTGTTGTGAATATTGAGTTGCAAATATGTCGGTGTTTG  >  NC_002947/1011174‑1011464                                                                                                                                                       |                                                                                                                                              gAAAATGCTGATGAAACCAGTCCCCAACCGAAGCGGGTTGCAGGGGAGTGGACGTCTCCGTGGGCGTAAAGGTTCCCATCTGAGGCGGCATTTGGCCAGTGTTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGATg                                                                                                                                                >  2:117529/1‑150 (MQ=255)                        ccAACCGAAGCGGGTTGCAGGGGAGTGGACGTCTCCGTGGGCGTAAAGGTTCCCATCTGAGGCGGCATTTGGCCAGTGTTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGATGTCTCAAATCATCTTTTAATGata                                                                                                                         >  2:530614/1‑150 (MQ=255)                        ccAACCGAAGCGGGTTGCAGGGGAGTGGACGTCTCCGTGGGCGTAAAGGTTCCCATCTGAGGCGGCATTTGGCCAGTGTTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGATGTCTCAAATCATCTTTTAATGata                                                                                                                         >  1:20031/1‑150 (MQ=255)                                aGCGGGTTGCAGGGGAGTGGACGTCTCCGTGGGCGTAAAGGTTCCCATCTGAGGCGGCATTTGGCCAGTGTTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGATGTCTCAAATCATCTTTTAATGATAATAATtct                                                                                                                 <  1:429192/150‑1 (MQ=255)                                  cGGGTTGCAGGGGAGTGGACGTCTCCGTGGGCGTAAAGGTTCCCATCTGAGGCGGCATTTGGCCAGTGTTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGATGTCTCAAATCATCTTTTAATGATAATAATTCTCa                                                                                                               <  1:598344/150‑1 (MQ=255)                                     gTTGCAGGGGAGTGGACGTCTCCGTGGGCGTAAAGGTTCCCATCTGAGGCGGCATTTGGCCAGTGTTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGATGTCTCAAATCATCTTTTAATGATAATAATTCTCATta                                                                                                            <  2:113484/150‑1 (MQ=255)                                       tGCAGGGGAGTGGACGTCTCCGTGGGCGTAAAGGTTCCCATCTGAGGCGGCATTTGGCCAGTGTTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGATGTCTCAAATCATCTTTTAATGATAATAATTCTCATtata                                                                                                          >  2:384332/1‑150 (MQ=255)                                                    aCGTCTCCGTGGGCTTATAGGTTCCCATGTGTGGCGGCTTTTGGCCAGTTTTAATCGCTGGTTTGCGGTTTTTTTGGGTATAAAATCAGCGACTTGATGTCTCAAATCATCTTTTAATGATATTAATTCTCCTTATATTGCCGAGACGaa                                                                                             <  2:57469/150‑1 (MQ=255)                                                        ctcCGTGGGCGTAAAGGTTCCCATCTGAGGCGGCATTTGGCCAGTGTTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGATGTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCaa                                                                                         <  2:489542/150‑1 (MQ=255)                                                        ctcCGTGGGCGTAAAGGTTCCCATCTGAGGCGGCATTTGGCCAGTGTTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGATGTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCaa                                                                                         <  2:489532/150‑1 (MQ=255)                                                               ggCGTAAAGGTTCCCATCTGAGGCGGCATTTGGCCAGTGTTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGATGTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCAACAGCAAt                                                                                  <  1:591987/150‑1 (MQ=255)                                                                  gTAAAGGTTCCCATCTGAGGCGGCATTTGGCCAGTGTTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGATGTCTGAAATCATCTTTTATTGATAATAATTCTCATTATATTGGCGCGACGAAGCAACAGAAATgat                                                                               >  2:463044/1‑148 (MQ=255)                                                                                       ggcATTTGGCCAGTGTTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGATGTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCAACAGCAATGCTTACGCATAACCATAGCGaaaa                                                          >  1:550901/1‑150 (MQ=255)                                                                                       ggcATGTGGCCAGTGTTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGATGTCTCAAATCTTCTTTTATTGAAAATAATTCTCATTATATTGCCGCGACGAAGCAACAGCAATGCTTACGCATAACCATAGCGtaaa                                                          >  1:243056/1‑150 (MQ=255)                                                                                          aTTTGGCCAGTGTTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGATGTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCAACAGCAATGCTTACGCATAACCATAGCGAAAATAg                                                       >  1:535867/1‑150 (MQ=255)                                                                                            ttGGCCAGTGTTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGATGTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCAACAGCAATGCTTACGCATAACCATAGCGAAAATAGTg                                                     <  2:550901/150‑1 (MQ=255)                                                                                            tcggCCCGTCTTAATCGCTGGGTGGCGGGATTTTGGGCGAGAAAAACAGCGACTTGAAGTCTCAAATCATCTTCTAATGATAATAAATCTCATAATATTGCCGCGACGACGCAACAGCAATGCTTACGCATAACCATAGCGAAAATAGTg                                                     <  2:243056/148‑1 (MQ=255)                                                                                                     gtTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGATGTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCAACAGCAATGCTTACGCATAACCATAGCGAAAATAGTGGCGCAgtgg                                            >  2:470291/1‑149 (MQ=255)                                                                                                        aaTCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGATGTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCAACAGCAATGCTTACGCATAACCATAGCGAAAATAGTGGCGCAGTGTAAg                                         <  2:409598/150‑1 (MQ=255)                                                                                                           cGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGATGTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCAACAGCAATGCTTACGCATAACCATAGCGAAAATAGTGGCGCAGTGTAAGgtt                                      <  1:114840/150‑1 (MQ=255)                                                                                                           cGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGATGTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCAACAGCAATGCTTACGCATAACCATAGCGAAAATAGTGGCGCAGTGTAAGgtt                                      <  1:177528/150‑1 (MQ=255)                                                                                                                ggTTGCGGTTTTTTGGGCGAGAAAAACAGCGACTTGATGTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCAACAGCAATGCTTACGCATAACCATAGCGAAAATAGTGGCGCAGTGTAAGGTtgttgt                                 <  1:62211/150‑1 (MQ=255)                                                                                                                ggTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGATGTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCAACAGCAATGCTTACGCATAACCATAGCGAAAATAGTGGCGCAGTGTAAGGTtgttgt                                 <  1:117529/150‑1 (MQ=255)                                                                                                                  ttGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGATGTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCAACAGCAATGCTTACGCATAACCATAGCGAAAATAGTGGCGCAGTGTAAGGTTGTTGTGa                               >  2:313720/1‑150 (MQ=255)                                                                                                                  ttGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGATGTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCAACAGCAATGCTTACGCATAACCATAGCGAAAATAGTGGCGCAGTGTAAGGTTGTTGTGa                               >  2:313716/1‑150 (MQ=255)                                                                                                                      gggTTTTTGGGCGAGAAAAACAGCGACTTGATGTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCAACAGCAATGCTTACGCATAACCATAGCGAAAATAGTGGCGCAGTGTAAGGTTGTTGTGAaaat                           >  2:32018/1‑150 (MQ=255)                                                                                                                                        aaCAGCGACTTGATGTCTCAAATCATCTTTTATTGATAATAATTCTCATTATATTGCCGCGACGAAGCAACAGCTATGCTTACGCATAACCATAGCGAAAATAGTGGCGCAGTGTAAGGTTGTTGTGAATATTGAGTTGCAAATATGTCg         <  1:419042/150‑1 (MQ=255)                                                                                                                                        aaCAGCGACTTGATGTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCAACAGCAATGCTTACGCATAACCATAGCGAAAATAGTGGCGCAGTGTAAGGTTGTTGTGAATATTGAGTTGCAAATATGTCg         <  1:297060/150‑1 (MQ=255)                                                                                                                                        aaCAGCGACTTGATGTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCAACAGCAATGCTTACGCATAACCATAGCGAAAATAGTGGCGCAGTGTAAGGTTGTTGTGAATATTGAGTTGCAAATATGTCg         <  1:297076/150‑1 (MQ=255)                                                                                                                                               aCTTGATGTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCAACAGCAATGCTTACGCATAACCATAGCGAAAATAGTGGCGCAGTGTAAGGTTGTTGTGAATATTGAGTTGCAAATATGTCGGTGTTTg  <  2:20031/150‑1 (MQ=255)                                                                                                                                                       |                                                                                                                                              GAAAATGCTGATGAAACCAGTCCCCAACCGAAGCGGGTTGCAGGGGAGTGGACGTCTCCGTGGGCGTAAAGGTTCCCATCTGAGGCGGCATTTGGCCAGTGTTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGATG‑CTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCAACAGCAATGCTTACGCATAACCATAGCGAAAATAGTGGCGCAGTGTAAGGTTGTTGTGAATATTGAGTTGCAAATATGTCGGTGTTTG  >  NC_002947/1011174‑1011464

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑