| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,167,912 | +A | coding (725/1344 nt) | oprB‑I → | carbohydrate‑selective porin |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,167,910 | 1 | . | A | 92.0% | 75.4 / 1.2 | 25 | coding (723/1344 nt) | oprB‑I | carbohydrate‑selective porin |
| Reads supporting (aligned to +/- strand): ref base . (2/0); new base A (8/15); total (10/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.50e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01 | |||||||||||
GTACAACTGGCCGGTCAGCCAGTGGGCGCTGCGGGTGAAGTACAACATCACGCCGGAGTTCTTCGTACAGGTCGGGGCCTTCGAGCAGAACCCTTCCAACCTGGAAACCGGCAACGGCTTCAAGCTCAGCGGCAGTGGTACC‑AAGGGGGCGATCTTGCCGGTGGAAGCGGTGTGGTCGCCCAAGGTCAATGGCCTGCCGGGCGAGTACCGCCTGGGTTACTACTACAGCACGGCCAAGGCTGACGATGTGTACGACGACGTCAACGGCAACCCGCAGGCGCTGACA > NC_002947/1167769‑1168054 | gTACAACTGGCCGGTCAGCCAGTGGGCGCTGCGGGTGAAGTACAACATCACGCCGGAGTTCTTCGTACAGGTCGGGGCCTTCGAGCAGAACCCTTCCAACCTGGAAACCGACAACGGCTTCAAGCTCAGCGGCAGTGGTACC‑AAaggggg > 1:268470/1‑149 (MQ=255) tACAACTGGCCGGTCAGCCAGTGGGCGCTGCGGGTGAAGTACAACATCACGCCGGAGTTCTTCGTACAGGTCGGGGCCTTCGAGCAGAACCCTTCCAACCTGGAAACCGGCAACGGCTTCAAGCTCAGCGGCAGTGGTACC‑AAagggggc > 1:149076/1‑148 (MQ=255) aCTGGCCGGTCAGCCAGTGGGCGCTGCGGGTGAAGTACAACATCACGCCGGAGTTCTTCGTACAGGTCGGGGCCTTCGAGCAGAACCCTTCCGACCTGGAAACCGGCAACGGCTTCAAGCTCAGCGGCAGTGGTACCAAAGGGGGCGATc < 1:464908/150‑1 (MQ=255) aCTGGCCGGTCAGCCAGTGGGCGCTGCGGGTGAAGTACAACATCACGCCGGAGTTCTTCGTACAGGTCGGGGCCTTCGAGCAGAACCCTTCCAACCTGGAAACCGGCAACGGCTTCAAGCTCAGCGGCAGTGGTACCAAAGGGGGCGATc < 1:187758/150‑1 (MQ=255) aCTGGCCGGTCAGCCAGTGGGCGCTGCGGGTGAAGTACAACATCACGCCGGAGTTCTTCGTACAGGTCGGGGCCTTCGAGCAGAACCCTTCCAACCTGGAAACCGGCAACGGCTTCAAGCTCAGCGGCAGTGGTACCAAAGGGGGCGATc < 1:464914/150‑1 (MQ=255) gCCAGTGGGCGCTGCGGGTGAAGTACAACATCACGCCGGAGTTCTTCGTACAGGTCGGGGCCTTCGAGCAGAACCCTTCCAACCTGGAAACCGGCAACGGCTTCAAGCTCAGCGGCAGTGGTACCAAAGGGGGCGATCTTGCCGGTGGaa < 1:350479/150‑1 (MQ=255) gTGGGCGCTGCGGGTGAAGTACAACATCACGCCGGAGTTCTTCGTACAGGTCGGGGCCTTCGAGCAGAACCCTTCCAACCTGGAAACCGGCAACGGCTTCAAGCTCAGCGGCAGTGGTACCAAAGGGGGCGATCTTGCCGGTGGAAGCgg > 2:610042/1‑150 (MQ=255) gggCGCTGCGGGTGAAGTACAACATCACGCCGGAGTTCTTCGTACAGGTCGGGGCCTTCGAGCAGAACCCTTCCAACCTGGAAACCGGCAACGGCTTCAAGCTCAGCGGCAGTGGTACCAAAGGGGGCGATCTTGCCGGTGGAAGCGgtg < 1:296183/150‑1 (MQ=255) ggCGCTGCGGGTGAAGTACAACATCACGCCGGAGTTCTTCGTACAGGTCGGGGCCTTCGAGCAGAACCCTTCCAACCTGGAAACCGGCAACGGCTTCAAGCTCAGCGGCAGTGGTACCAAAGGGGGCGATCTTGCCGGTGGAAGCGgtgt > 1:179137/1‑150 (MQ=255) tGAAGTACAACATCACGCCGGAGTTCTTCGTACAGGTCGGGGCCTTCGAGCAGAACCCTTCCAACCTGGAAACCGGCAACGGCTTCAAGCTCAGCGGCAGTGGTACCAAAGGGGGCGATCTTGCCGGTGGAAGCGGTGTGGTCGCCCAAg < 1:451704/150‑1 (MQ=255) gAAGTACAACATCACGCCGGAGTTCTTCGTACAGGTCGGGGCCTTCGAGCAGAACCCTTCCAACCTGGAAACCGGCAACGGCTTCAAGCTCAGCGGCAGTGGTACCAAAGGGGGCGATCTTGCCGGTGGAAGCGGTGTGGTCGCCCAAgg > 1:255306/1‑150 (MQ=255) gAAGTACAACATCACGCCGGAGTTCTTCGTACAGGTCGGGGCCTTCGAGCAGAACCCTTCCAACCTGGAAACCGGCAACGGCTTCAAGCTCAGCGGCAGTGGTACCAAAGGGGGCGATCTTGCCGGTGGAAGCGGTGTGGTCGCCCAAgg < 2:602887/150‑1 (MQ=255) acaTCACGCCGGAGTTCTTCGTACAGGTCGGGGCCTTCGAGCAGAACCCTTCCAACCTGGAAACCGGCAACGGCTTCAAGCTCAGCGGCAGTGGTACCAAAGGGGGCGATCTTGCCGGTGGAAGCGGTGTGGTCGCCCAAGGTCAATGGc < 2:293246/150‑1 (MQ=255) ccGGAGTTCTTCGTACAGGTCGGGGCCTTCGAGCAGAACCCTTCCAACCTGGAAACCGGCAACGGCTTCAAGCTCAGCGGCAGTGGTACCAAAGGGGGCGATCTTGCCGGTGGAAGCGGTGTGGTCGCCCAAGGTCAATGGCCTGCCggg > 1:117549/1‑150 (MQ=255) ttcttcGTACAGGTCGGGGCCTTCGAGCAGAACCCTTCCAACCTGGAAACCGGCAACGGCTTCAAGCTCAGCGGCAGTGGTACCAAAGGGGGCGATCTTGCCGGTGGAAGCGGTGTGGTCGCCCAAGGTCAATGGCCTGCCGGGCGAGTa < 2:377875/150‑1 (MQ=255) aCAGGTCGGGGCCTTCGAGCAGAACCCTTCCAACCTGGAAACCGGCAACGGCTTCAAGCTCAGCGGCAGTGGTACCAAAGGGGGCGATCTTGCCGGTGGAAGCGGTGTGGTCGCCCAAGGTCAATGGCCTGCCGGGCGAGTACCGCCTgg < 2:571416/150‑1 (MQ=255) aCAGGTCGGGGCCTTCGAGCAGAACCCTTCCAACCTGGAAACCGGCAACGGCTTCAAGCTCAGCGGCAGTGGTACCAAAGGGGGCGATCTTGCCGGTGGAAGCGGTGTGGTCGCCCAAGGTCAATGGCCTGCCGGGCGAGTACCGCCTgg < 2:263689/150‑1 (MQ=255) ttCGAGCAGAACCCTTCCAACCTGGAAACCGGCAACGGCTTCAAGCTCAGCGGCAGTGGTACCAAAGGGGGCGATCTTGCCGGTGGAAGCGGGGTGGTCGCCCAAGGTCAATGGCCTGCCGGGCGAGTACCGCCGGGGTTACTACTACAg > 2:88077/1‑150 (MQ=255) aGCAGAACCCTTCCAACCTGGAAACCGGCAACGGCTTCAAGCTCAGCGGCAGTGGTACCAAAGGGGGCGATCTTGCCGGTGGAAGCGGTGTGGTCGCCCAAGGTCAATGGCCTGCCGGGCGAGTACCGCCTGGGTTACTACTACAGCACg < 2:575916/150‑1 (MQ=255) aCCCTTCCAACCTGGAAACCGGCAACGGCTTCAAGCTCAGCGGCAGTGGTACCAAAGGGGGCGATCTTGCCGGTGGAAGCGGTGTGGTCGCCCAAGGTCAATGGCCTGCCGGGCGAGTACCGCCTGGGTTACTACTACAGCACGGCCAAg < 1:574000/150‑1 (MQ=255) aCCCTTCCAACCTGGAAACCGGCAACGGCTTCAAGCTCAGCGGCAGTGGTACCAAAGGGGGCGATCTTGCCGGTGGAAGCGGTGTGGTCGCCCAAGGTCAATGGCCTGCCGGGCGAGTACCGCCTGGGTTACTACTACAGCACGGCCAAg < 1:266262/150‑1 (MQ=255) ttCCAACCTGGAAACCGGCAACGGCTTCAAGCTCAGCGGCAGTGGTACCAAAGGGGGCGATCTTGCCGGTGGAAGCGGTGTGGTCGCCCAAGGTCAATGGCCTGCCGGGCGAGTACCGCCTGGGTTACTACTACAGCACGGCCAAGGCTg > 1:282080/1‑150 (MQ=255) aaCCGGCAACGGCTTCAAGCTCAGCGGCAGTGGTACCAAAGGGGGCGATCTTGCCGGTGGAAGCGGTGTGGTCGCCCAAGGTCAATGGCCTGCCGGGCGAGTACCGCCTGGGTTACTACTACAGCACGGCCAAGGCTGACGATGTGTacg > 2:164219/1‑150 (MQ=255) gCGGCAGTGGTACCAAAGGGGGCGATCTTGCCGGTGGAAGCGGTGTGGTCGCCCAAGGTCAATGGCCTGCCGGGCGAGTACCGCCTGGGTTACTACTACAGCACGGCCAAGGCTGACGATGTGTACGACGACGTCAACGGCAACCCGCAg > 1:411118/1‑150 (MQ=255) gTACCAAAGGGGGCGATCTTGCCGGTGGAAGCGGTGTGGTCGCCCAAGGTCAATGGCCTGCCGGGCGAGTACCGCCTGGGTTACTACTACAGCACGGCCAAGGCTGACGATGTGTACGACGACATCAACGGCAACCCGCAGGCGCTGACa < 2:280142/150‑1 (MQ=255) | GTACAACTGGCCGGTCAGCCAGTGGGCGCTGCGGGTGAAGTACAACATCACGCCGGAGTTCTTCGTACAGGTCGGGGCCTTCGAGCAGAACCCTTCCAACCTGGAAACCGGCAACGGCTTCAAGCTCAGCGGCAGTGGTACC‑AAGGGGGCGATCTTGCCGGTGGAAGCGGTGTGGTCGCCCAAGGTCAATGGCCTGCCGGGCGAGTACCGCCTGGGTTACTACTACAGCACGGCCAAGGCTGACGATGTGTACGACGACGTCAACGGCAACCCGCAGGCGCTGACA > NC_002947/1167769‑1168054 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |