Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	233,545	C→T	G548G (GGC→GGT)	PP_0180 →	cytochrome c family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	233,545	0	C	T	100.0%	90.1 / NA	27	G548G (GGC→GGT)	PP_0180	cytochrome c family protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/13);  total (14/13)

TCGGTATACCGCGAATTGTTCGAAGTGATCCTGTTCTACGAGACCCTGTGGCTGCAGGCAGGCCCCGCCGGGCATCAGGCGGTGCTGGCGGGTGGCGCGACGGCACTGGTGCTGTTGGTGGGCCTGGCCTGGGTGATTCTGCGGGGCTCGGCCAAGCTGCCGCTATCGCTGTTCTTCAGCATCAACGCCGCGCTGCTGTGCGCGCTGTCGGTAGTATTCGCCGGGCATGGCGTGAAGGCGTTGCAAGAGGCCGGTGTGCTGGGCACACGGCCAGTGGCGTTCTTTGAGT  >  NC_002947/233399‑233687                                                                                                                                                   |                                                                                                                                               tcGGTATACCGCGAATTGTTCGAAGTGATCCTGTTCTACGAGACCCTGTGGCTGCAGGCAGGCCCCGCCGGGCATCAGGCGGTGCTGGCGGGTGGCGCGACGGCACTGGTGCTGTTGGTGGGCCTGGCCTGGGTGATTCTGCGGGGTTCg                                                                                                                                             >  2:112501/1‑150 (MQ=255)      ataCCGCGAATTGTTCGAAGTGATCCTGTTCTACGAGACCCTGTGGCTGCAGGCAGGCCCCGCCGGGCATCAGGCGGTGCTGGCGGGTGGCGCGACGGCACTGGTGCTGTTGGTGGGCCTGGCCTGGGTGATTCTGCGGGGTTCGGCCaa                                                                                                                                        <  2:214131/150‑1 (MQ=255)                      gAAGTGATCCTGTTCTACGAGACCCTGTGGCTGCAGGCAGGCCCCGCCGGGCATCAGGCGGTGCTGGCGGGTGGCGCGACGGCACTGGTGCTGTTGGTGGGCCTGGCCTGGGTGATTCTGCGGGGTTCGGCCAAGCTGCCGCTATCGCTg                                                                                                                        >  1:18918/1‑150 (MQ=255)                        aGTGATCCTGTTCTACGAGACCCTGTGGCTGCAGGCAGGCCCCGCCGGGCATCAGGCGGTGCTGGCGGGTGGCGCGACGGCACTGGTGCTGTTGGTGGGCCTGGCCTGGGTGATTCTGCGGGGTTCGGCCAAGCTGCCGCTATCGCTGtt                                                                                                                      <  2:460579/150‑1 (MQ=255)                            aTCCTGTTCTACGAGACCCTGTGGCTGCAGGCAGGCCCCGCCGGGCATCAGGCGGTGCTGGCGGGTGGCGCGACGGCACTGGTGCTGTTGGTGGGCCTTGCCTGGGTGATTCTGCGGGGTTCGGCCAAGCTGCCGCTATCGCTGttcttc                                                                                                                  <  1:458577/150‑1 (MQ=255)                            aTCCTGTTCTACGAGACCCTGTGGCTGCAGGCAGGCCCCGCCGGGCATCAGGCGGTGCTGGCGGGTGGCGCGACGGCACTGGTGCTGTTGGTGGGCCTGGCCTGGGTGATTCTGCGGGGTTCGGCCAAGCTGCCGCTATCGCTGttcttc                                                                                                                  <  1:458562/150‑1 (MQ=255)                             tCCTGTTCTACGAGACCCTGTGGCTGCAGGCAGGCCCCGCCGGGCATCAGGCGGTGCTGGCGGGTGGCGCGACGGCACTGGTGCTGTTGGTGGGCCTGGCCTGGGTGATTCTGCGGGGTTCGGCCAAGCTGCCGCTATCGCTGTTCTTCa                                                                                                                 <  1:112736/150‑1 (MQ=255)                                    cTACGAGACCCTGTGGCTGCAGGCAGGCCCCGCCGGGCATCAGGCGGTGCTGGCGGGTGGCGCGACGGCACTGGTGCTGTTGGTGGGCCTGGCCTGGGTGATTCTGCGGGGTTCGGCCAAGCTGCCGCTATCGCTGTTCTTCAGCATCaa                                                                                                          <  1:353206/150‑1 (MQ=255)                                       cGAGACCCTGTGGCTGCAGGCAGGCCCCGCCGGGCATCAGGCGGTGCTGGCGGGTGGCGCGACGGCACTGGTGCTGTTGGTGGGCCTGGCCTGGGTGATTCTGCGGGGTTCGGCCAAGCTGCCGCTATCGCTGTTCTTCAGCATCAAcgc                                                                                                       >  2:144216/1‑150 (MQ=255)                                        gagaCCCTGTGGCTGCAGGCAGGCCCCGCCGGGCATCAGGCGGTGCTGGCGGGTGGCGCGACGGCACTGGTGCTGTTGGTGGGCCTGGCCTGGGTGATTCTGCGGGGTTCGGCCAAGCTGCCGCTATCGCTGTTCTTCAGCATCAAcgcc                                                                                                      >  1:247976/1‑150 (MQ=255)                                                  ggCTGCAGGCAGGCCCCGCCGGGCATCAGGCGGTGCTGGCGGGTGGCGCGACGGCACTGGTGCTGTTGGTGGGCCTGGCCTGGGTGATTCTGCGGGGTTCGGCCAAGCTGCCGCTATCGCTGTTCTTCAGCATCAACGCCGCGCTGCtgt                                                                                            <  2:335328/150‑1 (MQ=255)                                                    cTGCAGGCAGGCCCCGCCGGGCATCAGGCGGTGCTGGCGGGTGGCGCGACGGCACTGGTGCTGTTGGTGGGCCTGGCCTGGGTGATTCTGCGGGGTTCGGCCAAGCTGCCGCTATCGCTGTTCTTCAGCATCAACGCCGCGCTGCTGTgc                                                                                          <  1:473309/150‑1 (MQ=255)                                                     tGCAGGCAGGCCCCGCCGGGCATCAGGCGGTGCTGGCGGGTGGCGCGACGGCACTGGTGCTGTTGGTGGGCCTGGCCTGGGTGATTCTGCGGGGTTCGGCCAAGCTGCCGCTATCGCTGTTCTTCAGCATCAACGCCGCGCTGCTGGgcg                                                                                         >  2:199307/1‑150 (MQ=255)                                                            aggcCCCGCCGGGCATCAGGCGGTGCTGGCGGGTGGCGCGACGGCACTGGTGCTGTTGGTGGGCCTGGCCTGGGTGATTCTGCGGGGTTCGGCCAAGCTGCCGCTATCGCTGTTCTTCAGCATCAACGCCGCGCTGCTGTGCGCGCTGTc                                                                                  >  1:345435/1‑150 (MQ=255)                                                                cccGCCGGGCATCAGGCGGTGCTGGCGGGTGGCGCGACGGCACTGGTGCTGTTGGTGGGCCTGGCCTGGGTGATTCTGCGGGGTTCGGCCAAGCTGCCGCTATCGCTGTTCTTCAGCATCAACGCCGCGCTGCTGTGCGCGCTGTCGgta                                                                              <  1:98092/150‑1 (MQ=255)                                                                       agCATCAGGCGGTGCTGGCGGGTGGCGCGACGGCACTGGTGCTGTTGGTGGGCCTGGCCTGGGTGATTCTGCGGGGTTCGGCCAAGCTGCCGCTATCGCTGTTCTTCAGCATCAACGCCGCGCTGCTGTGCGCGCTGTCGGTAGTATTCg                                                                       >  1:424992/2‑150 (MQ=255)                                                                                          gggTGGCGCGACGGCACTGGTGCTGTTGGTGGGCCTGGCCTGGGTGATTCTGCGGGGTTCGGCCAAGCTGCCGCTATCGCTGTTCTTCAGCATCAACGCCGCGCTGCTGTGCGCGCTGTCGGTAGTATTCGCCGGGCATGGCGTGAAGGc                                                    >  2:487410/1‑150 (MQ=255)                                                                                            gTGGCGCGACGGCACTGGTGCTGTTGGTGGGCCTGGCCTGGGTGATTCTGCGGGGTTCGGCCAAGCTGCCGCTATCGCTGTTCTTCAGCATCAACGCCGCGCTGCTGTGCGCGCTGTCGGTAGTATTCGCCGGGCATGGCGTGAAGGCGt                                                  <  2:345435/150‑1 (MQ=255)                                                                                                         aCTGGTGCTGTTGGTGGGCCTGGCCTGGGTGATTCTGCGGGGTTCGGCCAAGCTGCCGCTATCGCTGTTCTTCAGCATCAACGCCGCGCTGCTGTGCGCGCTGTCGGTAGTATTCGCCGGGCATGGCGTGAAGGCGTTGCAAGAGGCCgg                                     >  1:385591/1‑150 (MQ=255)                                                                                                              tGCTGTTGGTGGGCCTGGCCTGGGTGATTCTGCGGGGTTCGGCCAAGCTGCCGCTATCGCTGTTCTTCAGCATCAACGCCGCGCTGCTGTGAGCGCTGTCGGTAGTATTCGCCGGGCATGGCGTGAAGGCGTTGCAAGAGGCCGGTGTGc                                >  1:188941/1‑150 (MQ=255)                                                                                                                   ttGGTGGGCCTGGCCTGGGTGATTCTGCGGGGTTCGGCCAAGCTGCCGCTATCGCTGTTCTTCAGCATCAACGCCGCGCTGCTGTGCGCGCTGTCGGTAGTCTTCGCCGGGCATGGCGTGAAGGCGTTGCAAGAGGCCGGTGTGCTGGGc                           >  2:522551/1‑150 (MQ=255)                                                                                                                            ctggcctggGTGATTCTGCGGGGTTCGGCCAAGCTGCCGCTATCGCTGTTCTTCAGCATCAACGCCGCGCTGCTGTGCGCGCTGTCGGTAGTATTCGCCGGGCATGGCGTGAAGGCGTTGCAAGAGGCCGGTGTGCTGGGCACACGGCCa                  >  1:53850/1‑150 (MQ=255)                                                                                                                               gcctgtGTGATTCTGCGGGGTTCGGCCAAGCTGCCGCTATCGCTGTTCTTCAGCATCAACGCCGCGCTGCTGTGCGCGCTGTCGGTAGTATTCGCCGGGCATGGCGTGAAGGCGTTGCAAGAGGCCGGTGTGCTGGGCACACGGCCAGTg               <  1:156792/150‑1 (MQ=255)                                                                                                                               gcctggGTGATTCTGCGGGGTTCGGCCAAGCTGCCGCTATCGCTGTTCTTCAGCATCAACGCCGCGCTGCTGTGCGCGCTGTCGGTAGTATTCGCCGGGCATGGCGTGAAGGCGTTGCAAGAGGCCGGTGTGCTggg                            <  2:329933/137‑1 (MQ=255)                                                                                                                               gcctggGTGATTCTGCGGGGTTCGGCCAAGCTGCCGCTATCGCTGTTCTTCAGCATCAACGCCGCGCTGCTGTGCGCGCTGTCGGTAGTATTCGCCGGGCATGGCGTGAAGGCGTTGCAAGAGGCCGGTGTGCTggg                            >  1:329933/1‑137 (MQ=255)                                                                                                                                    ggTGATTCTGCGGGGTTCGGCCAAGCTGCCGCTATCGCTGTTCTTCAGCATCAACGCCGCGCTGCTGTGCGCGCTGTCGGTAGTATTCGCCGGGCATGGCGTGAAGGCGTTGCAAGAGGCCGGTGTGCTGGGCACACGGCCAGTGGCGtt          <  2:424992/150‑1 (MQ=255)                                                                                                                                            tGCGGGGTTCGGCCAAGCTGCCGCTATCGCTGTTCTTCAGCATCAACGCCGCGCTGCTGTGCGCGCTGTCGGTAGTATTCGCCGGGCATGGCGTGAAGGCGTTGCAAGAGGCCGGTGTGCTGGGCACACGGCCAGTGGCGTTCTTTGAGt  >  1:426946/1‑150 (MQ=255)                                                                                                                                                   |                                                                                                                                               TCGGTATACCGCGAATTGTTCGAAGTGATCCTGTTCTACGAGACCCTGTGGCTGCAGGCAGGCCCCGCCGGGCATCAGGCGGTGCTGGCGGGTGGCGCGACGGCACTGGTGCTGTTGGTGGGCCTGGCCTGGGTGATTCTGCGGGGCTCGGCCAAGCTGCCGCTATCGCTGTTCTTCAGCATCAACGCCGCGCTGCTGTGCGCGCTGTCGGTAGTATTCGCCGGGCATGGCGTGAAGGCGTTGCAAGAGGCCGGTGTGCTGGGCACACGGCCAGTGGCGTTCTTTGAGT  >  NC_002947/233399‑233687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑