BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	4,983,564	(C)_8→7	coding (30/696 nt)	flgH ←	flagellar L‑ring protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,983,557	0	C	.	99.7%	1708.6 / ‑3.0	354	coding (37/696 nt)	flgH	flagellar L‑ring protein
Reads supporting (aligned to +/- strand): ref base C (0/0); major base . (160/193); minor base T (1/0); total (161/193)
Fisher's exact test for biased strand distribution p-value = 4.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 375 total aligned reads displayed.

GCGGTCGCTGTACAGGTTCTGCTCGAAACCGGCCTGGTAGATCGAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCGCCCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACGACAGCATCTGGTCGGCCGTGGAAATGACTTTGGAGTTCATCTCGTAGG  >  NC_002947/4983408‑4983700

gCGGTCGCTGTACAGGTTCTGCTCGAAACCGGCCTGGTAGATCGAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCGc                                                                                                                                                 >  1:1764175/1‑150 (MQ=255)

   gTCGCTGTACAGGTTCTGCTCGAAACCGGCCTGGTAGATCGAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCGcccc                                                                                                                                              <  2:5648660/150‑1 (MQ=255)

     cGCTGTACAGGTTCTGCTCGAAACCGGCCTGGTAGATCGAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCGcccccc                                                                                                                                            <  2:2398906/150‑1 (MQ=255)

       cTGTACAGGTTCTGCTCGAAACCGGCCTGGTAGATCGAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCGccccccca                                                                                                                                          <  1:3586754/150‑2 (MQ=255)

          tACAGGTTCTGCTCGAAACCGGCCTGGTAGATCGAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCGcccccccaggg                                                                                                                                       >  1:4398848/1‑146 (MQ=255)

          tACAGGTTCTGCTCGAAACCGGCCTGGTAGATCGAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCGcccccccaggg                                                                                                                                       >  1:6521220/1‑146 (MQ=255)

          tACAGGTTCTGCTCGAAACCGGCCTGGTAGATCGAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCGcccccccaggg                                                                                                                                       <  1:2990907/150‑5 (MQ=255)

           aCAGGTTCTGCTCGAAACCGGCCTGGTAGATCGAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGc                                                                                                                                     >  2:2674410/1‑150 (MQ=255)

            cAGGTTCTGCTCGAAACCGGCCTGGTAGATCGAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCg                                                                                                                                    >  1:3888/1‑150 (MQ=255)

            cAGGTTCTGCTCGAAACCGGCCTGGTAGATCGAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCg                                                                                                                                    >  2:5256342/1‑150 (MQ=255)

             aGGTTCTGCTCGAAACCGGCCTGGTAGATCGAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGa                                                                                                                                   >  2:274542/1‑150 (MQ=255)

                ttCTGCTCGAAACCGGCCTGGTAGATCGAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGaaaa                                                                                                                                >  1:972247/1‑150 (MQ=255)

                   tGCTCGAAACCGGCCTGGTAGATCGAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCggtgttgc                                                                                                                             <  2:4054320/150‑8 (MQ=255)

                    gCTCGAAACGGGCCTGGTAGATCGAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGa                                                                                                                            <  1:3235688/150‑1 (MQ=255)

                    gCTCGAAACCGGCCTGGTAGATCGAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGa                                                                                                                            <  2:4830285/150‑1 (MQ=255)

                      tCGAAACCGGCCTGGTAGATCGAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCGcccccccagg                                                                                                                                        <  2:1783747/137‑4 (MQ=255)

                      tCGAAACCGGCCTGGTAGATCGAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGaca                                                                                                                          >  2:262068/1‑150 (MQ=255)

                      tCGAAACCGGCCTGGTAGATCGAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGaca                                                                                                                          >  1:5379491/1‑150 (MQ=255)

                      tCGAAACCGGCCTGGTAGATCGAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGaca                                                                                                                          >  1:5308415/1‑150 (MQ=255)

                         aaaCCGGCCTGGTAGATCGAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGacaaca                                                                                                                       <  2:1159127/150‑1 (MQ=255)

                         aaaCCGGCCTGGTAGATCGAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGacaaca                                                                                                                       <  1:1948156/150‑1 (MQ=255)

                           aCCGGCCTGGTAGATCGAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGa                                                                                                                     <  1:291706/150‑1 (MQ=255)

                                  tGGTAGATCGAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCa                                                                                                              <  1:1172182/150‑1 (MQ=255)

                                    gTAGATCGAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATg                                                                                                            >  1:7887559/1‑150 (MQ=255)

                                    gTAGATCGAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATg                                                                                                            <  1:397166/150‑1 (MQ=255)

                                       gATCGAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGaca                                                                                                         >  1:1786969/1‑150 (MQ=255)

                                         tCGAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACt                                                                                                       >  2:7491583/1‑150 (MQ=255)

                                           gAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTa                                                                                                     <  2:6492645/150‑1 (MQ=255)

                                           gAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTa                                                                                                     <  2:2364739/150‑1 (MQ=255)

                                            aaCCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTAc                                                                                                    >  1:8050653/1‑150 (MQ=255)

                                                  tgttgGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACg                                                                                              <  2:6154827/150‑1 (MQ=255)

                                                   gttgGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACgg                                                                                             <  2:1482255/150‑1 (MQ=255)

                                                   gttgGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACgg                                                                                             <  2:455849/150‑1 (MQ=255)

                                                       cccGTTGCCGGCAGCGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGgcgc                                                                                         >  1:4500893/2‑150 (MQ=255)

                                                        ccGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCa                                                                                        <  2:8058244/150‑1 (MQ=255)

                                                             gCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGa                                                                                   <  2:7790085/150‑1 (MQ=255)

                                                             gCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGATGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGa                                                                                   <  2:4352615/150‑1 (MQ=255)

                                                              ccGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAg                                                                                  >  2:1225892/1‑150 (MQ=255)

                                                               cGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGt                                                                                 <  1:253650/150‑1 (MQ=255)

                                                               cGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGt                                                                                 <  2:1575592/150‑1 (MQ=255)

                                                                   aaCGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACa                                                                             >  2:3128073/1‑150 (MQ=255)

                                                                   aaCGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACa                                                                             >  2:145546/1‑150 (MQ=255)

                                                                     cGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAgg                                                                           <  2:6018556/150‑1 (MQ=255)

                                                                        ggTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCt                                                                        <  2:6764410/150‑1 (MQ=255)

                                                                          tGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACGGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTa                                                                      <  2:170341/150‑1 (MQ=255)

                                                                          tGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTa                                                                      <  1:7413920/150‑1 (MQ=255)

                                                                                gCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCCGCAACACCG‑CCCCCCCAGGGCGAACACGGACAACAGACGAGTCCTGACACTTACCTCACGGGCGCAGCTGAGTGACCGGGCGTAGAGCTg                                                                >  2:143910/1‑150 (MQ=255)

                                                                                  aaCACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGtt                                                              <  2:7917485/150‑1 (MQ=255)

                                                                                  aaCACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGtt                                                              <  2:1901880/150‑1 (MQ=255)

                                                                                  aaCACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGtt                                                              <  1:1225892/150‑1 (MQ=255)

                                                                                  aaCACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGtt                                                              <  1:4068706/150‑1 (MQ=255)

                                                                                  aaCACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGtt                                                              <  1:262068/150‑1 (MQ=255)

                                                                                   acacCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTg                                                             <  2:7636744/150‑1 (MQ=255)

                                                                                    cacCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACACCAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGa                                                            <  1:872988/150‑1 (MQ=255)

                                                                                    cacCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGa                                                            <  2:3667403/150‑1 (MQ=255)

                                                                                    cacCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGa                                                            <  1:2733478/150‑1 (MQ=255)

                                                                                      ccGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCa                                                                                        >  1:1583235/1‑120 (MQ=255)

                                                                                      ccGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGt                                                          <  2:3826219/150‑1 (MQ=255)

                                                                                       cGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTa                                                         >  1:4663364/1‑150 (MQ=255)

                                                                                       cGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTa                                                         >  1:7294801/1‑150 (MQ=255)

                                                                                         gcgcATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTaac                                                       <  2:7560454/150‑1 (MQ=255)

                                                                                           gcATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTaacga                                                     >  2:1826489/1‑150 (MQ=255)

                                                                                            cATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTaacgaa                                                    >  1:4619056/1‑150 (MQ=255)

                                                                                                 tACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACgg                                                                                             <  2:3444881/104‑1 (MQ=255)

                                                                                                    gggTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACGACAGCa                                            <  2:6362465/150‑1 (MQ=255)

                                                                                                    gggTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACGACAGCa                                            >  1:4891200/1‑150 (MQ=255)

                                                                                                      gTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCACCACCG‑CCCCCCCAGGGCGAAAACGGACAACCGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACGACAGCagc                                          >  1:5633353/1‑148 (MQ=255)

                                                                                                      gTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACGACAGCATc                                          <  1:3366055/150‑1 (MQ=255)

                                                                                                         gTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGCGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACGACAGCATCTgg                                       >  1:7791182/1‑150 (MQ=255)

                                                                                                               cttgggcGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGAtt                                                                                                                <  1:75117/71‑1 (MQ=255)

                                                                                                                 tgggcGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACGACAGCATCTGGTCGGCCGt                               <  2:2918183/150‑1 (MQ=255)

                                                                                                                    gcGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTaacgaac                                                   >  2:7099665/1‑127 (MQ=255)

                                                                                                                    gcGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTaacgaac                                                   <  1:7099665/127‑1 (MQ=255)

                                                                                                                    gcGTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACGACAGCATCTGGTCGGCCGTGGa                            >  2:5952621/1‑150 (MQ=255)

                                                                                                                      gTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACGACAGCATCTGGTCGGCCGTGGaaa                          <  1:6425268/150‑1 (MQ=255)

                                                                                                                      gTCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACGACAGCATCTGGTCGGCCGTGGaaa                          >  1:5801940/1‑150 (MQ=255)

                                                                                                                       tCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACGACAGCATCTGGTCGGCCGTGGAAAt                         <  2:2526641/150‑1 (MQ=255)

                                                                                                                       tCGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACGACAGCATCTGGTCGGCCGTGGAAAt                         <  2:7973160/150‑1 (MQ=255)

                                                                                                                        cGGTGCGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACGACAGCATCTGGTCGGCCGTGGAAATg                        >  2:4161940/1‑150 (MQ=255)

                                                                                                                             cGACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACGACAGCATCTGGTCGGCCGTGGAAATGACttt                   <  1:6984487/150‑1 (MQ=255)

                                                                                                                              gACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAgg                                                                           <  2:3239476/93‑1 (MQ=255)

                                                                                                                              gACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACGACAGCATCTGGTCGGCCGTGGAAATGACTTTg                  <  2:848615/150‑1 (MQ=255)

                                                                                                                              gACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACGACAGCATCTGGTCGGCCGTGGAAATGACTTTg                  >  1:7738771/1‑150 (MQ=255)

                                                                                                                              gACGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACGACAGCATCTGGTCGGCCGTGGAAATGACTTTg                  <  1:7574698/150‑1 (MQ=255)

                                                                                                                               aCGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACGACAGCATCTGGTCGGCCGTGGAAATGACTTTgg                 >  2:5693397/1‑150 (MQ=255)

                                                                                                                                cGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACGACAGCATCTGGTCGGCCGTGGAAATGACTTTGGa                <  1:4217261/150‑1 (MQ=255)

                                                                                                                                cGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACGACAGCATCTGGTCGGCCGTGGAAATGACTTTGGa                <  2:1094488/150‑1 (MQ=255)

                                                                                                                                cGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACGACAGCATCTGGTCGGCCGTGGAAATGACTTTGGa                <  1:1412614/150‑1 (MQ=255)

                                                                                                                                cGCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACGACAGCATCTGGTCGGCCGTGGAAATGACTTTGGa                <  2:7318562/150‑1 (MQ=255)

                                                                                                                                 gCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACGACAGCATCTGGTCGGCCGTGGAAATGACTTTGGAg               >  1:3734619/1‑150 (MQ=255)

                                                                                                                                 gCAACCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGCACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACCGGGCTTAGAGCTGTAGAGTAACGAACGACAGCAGCTCGTCGGCCGTGGACATGACTTTGGAg               >  2:3001884/1‑150 (MQ=255)

                                                                                                                                   aaCCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACGACAGCATCTGGTCGGCCGTGGAAATGACTTTGGAGtt             >  1:5090402/1‑150 (MQ=255)

                                                                                                                                    aCCTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACGACAGCATCTGGTCGGCCGTGGAAATGACTTTGGAGTTc            >  2:387409/1‑150 (MQ=255)

                                                                                                                                     ccTGCCAGCAACACCG‑CCCCCCCAGGCCGAAAACGGACAACAGACGATTCATGACCCTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACGACAGCATCTGGTCGGCCGTGGAAATGACTTTGGAGTTCa           <  1:1461376/150‑1 (MQ=255)

                                                                                                                                      cTGCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACGACAGCATCTGGTCGGCCGTGGAAATGACTTTg                  <  2:5584530/142‑1 (MQ=255)

                                                                                                                                        gCCAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTaac                                                       <  1:6793900/103‑1 (MQ=255)

                                                                                                                                         ccAGCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACTACAGCATCTGGTCGGCCGTGGAAATGACTTTGGAGTTCAtctc       >  1:4309001/1‑150 (MQ=255)

                                                                                                                                            gCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACGACAGCATCTGGTCGGCCGTGGAAATGACTTTGGAGTTCATCTCGTa    <  1:6224137/150‑1 (MQ=255)

                                                                                                                                            gCAACACCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACGACAGCATCTGGTCGGCCGTGGAAATGACTTTGGAGTTCATCTCGTa    <  2:3894404/150‑1 (MQ=255)

                                                                                                                                               acacCG‑CCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACGACAGCATCTGGTCGGCCGTGGAAATGACttt                   <  2:6974086/132‑1 (MQ=255)

GCGGTCGCTGTACAGGTTCTGCTCGAAACCGGCCTGGTAGATCGAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCGCCCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACGACAGCATCTGGTCGGCCGTGGAAATGACTTTGGAGTTCATCTCGTAGG  >  NC_002947/4983408‑4983700

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`