Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,018,2000AT41.7% 217.2 / inf 283intergenic (+76/+55)prfC/potF‑Ipeptide chain release factor 3/putrescine‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (76/85);  new base T (64/51);  total (147/136)
Fisher's exact test for biased strand distribution p-value = 1.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.11e-02
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 287 total aligned reads displayed.

CCCGGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAGCTTGCCCATCACCT  >  NC_002947/1018051‑1018349
                                                                                                                                                     |                                                                                                                                                      
cccGGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCa                                                                                                                                                        >  2:2037293/1‑150 (MQ=255)
 ccGGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCAt                                                                                                                                                       >  2:3806930/1‑150 (MQ=255)
 ccGGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCAt                                                                                                                                                       <  1:2913091/150‑1 (MQ=255)
  cGGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCAtg                                                                                                                                                      <  2:7873980/150‑1 (MQ=255)
   gggTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCAtgt                                                                                                                                                     <  2:432800/150‑1 (MQ=255)
   gggTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCAtgt                                                                                                                                                     <  1:3806930/150‑1 (MQ=255)
   gggTCAAACTGGCGCTGATGTAAGAGCTCTTTCCGGACATCAAGTTACGCGCTACCCTCGAGCACCTTTAAGGCCTGAAATCGTGGCTGCTTTGCAGCCCTTCTCGGGCACTCACGCTCCCACCGGAATCACCAGACCTGGAGGTCAtgt                                                                                                                                                     <  2:2118802/150‑1 (MQ=255)
     gTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTATCACATGACCTCCAGGTCTGGTGa                                                                                                                                                   >  1:7404563/1‑150 (MQ=37)
     gTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGa                                                                                                                                                   >  2:4234854/1‑150 (MQ=255)
      tCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTATCACATGACCTCCAGGTCTGGTGAt                                                                                                                                                  <  2:398679/150‑1 (MQ=37)
      tCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTATCACATGACCTCCAGGTCTGGTGAt                                                                                                                                                  <  1:6223412/150‑1 (MQ=37)
      tCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTATCACATGACCTCCAGGTCTGGTGAt                                                                                                                                                  <  1:1353029/150‑1 (MQ=37)
        aaCCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATAc                                                                                                                                                <  1:2593215/150‑1 (MQ=255)
         aCCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATAcc                                                                                                                                               <  1:7873734/150‑1 (MQ=255)
          ccTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACCGGAATCACCAGACCTGGAGGTCATGTGATACCt                                                                                                                                              <  2:53794/150‑1 (MQ=255)
          ccTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTATCACATGACCTCCAGGTCTGGTGATTCCt                                                                                                                                              <  1:1495369/150‑1 (MQ=25)
           cTGGCGCTGATGGAAGAGCGCTGGCCGGACATCATGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCCGCCTAGCGGCCCTTCGCGGGCACGCCCGCTCCCACAGGTATCACATGACCTCCAGGTCTGGTGATTCCtg                                                                                                                                             <  2:2385420/150‑1 (MQ=17)
           cTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTATCACATGACCTCCAGGTCTGGTGATTCCtg                                                                                                                                             <  1:4172048/150‑1 (MQ=25)
            tGGCGCTGATTGAAGAGCGCTTGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGTAATCGGGGCTTCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTATCACATGACCTCCAGGTCTGGTGATTCCtgt                                                                                                                                            <  2:1600664/150‑1 (MQ=21)
            tGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTATCACATGACCTCCAGGTCTGGTGATTCCtgt                                                                                                                                            <  2:1486020/150‑1 (MQ=25)
            tGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTATCACATGACCTCCAGGTCTGGTGATTCCtgt                                                                                                                                            <  2:7130705/150‑1 (MQ=25)
              gcgcTGATGGACGAGCGCTGGCCGGACCTCAAGGTCCGCGCTACCCGCGAGCACCCTTAAGGCCTGAACTCGGGGCTGCTTTGCAGCCGTTCGCGGGCACGCCCGCTCCCCCCGGTATCACATGACCTCGAGGTCTGGTGATTCCTGTgg                                                                                                                                          <  2:5927709/150‑1 (MQ=21)
              gcgcTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTATCACATGACCTCCAGGTCTGGTGATTCCTGTgg                                                                                                                                          <  1:4456881/150‑1 (MQ=25)
              gcgcTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCAtgt                                                                                                                                                     <  1:3359535/139‑1 (MQ=255)
                gcTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTATCACATGACCTCCAGGTCTGGTGATTCCTGTGGGa                                                                                                                                        <  2:1515271/150‑1 (MQ=21)
                gcTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGa                                                                                                                                        <  2:7490536/150‑1 (MQ=255)
                     tGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCggg                                                                                                                                   <  1:5485188/150‑1 (MQ=255)
                      ggAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCGGCCCTTCGCGGGCACGCCCGCTCCCACAGGTATCACATGACCTCCAGGTCTGGTGATTCCTGTgg                                                                                                                                          <  2:3371866/142‑1 (MQ=21)
                      ggAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCCTAGCGGCCCTTCGCGGGCACGCCCGCTCCCACAGGGATCACATGACCTCCCGGGCTGGTGATTGCGGTGGGAGCCGGc                                                                                                                                  >  2:6661052/1‑150 (MQ=18)
                        aaGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGt                                                                                                                                <  2:5614754/150‑1 (MQ=255)
                        aaGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCA‑CATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTATCACATGACCTCCAGGTCTGGTGATTCCtgt                                                                                                                                            >  2:4934524/1‑137 (MQ=21)
                        aaGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCA‑CATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTATCACATGACCTCCAGGTCTGGTGATTCCtgt                                                                                                                                            <  1:4934524/137‑1 (MQ=21)
                         agagCGCTGGCCGGACTTCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTg                                                                                                                               <  2:4139743/150‑1 (MQ=255)
                          gagCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCAtg                                                                                                                                                      <  1:508356/126‑1 (MQ=255)
                                        cATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCTGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGc                                                                                                                <  2:4923281/150‑1 (MQ=255)
                                          tCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTATCACATGACCTCCAGGTCTGGTGATTCCtg                                                                                                                                             <  2:1926637/119‑1 (MQ=18)
                                          tCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTATCACATGACCTCCAGGTCTGGTGATTCCtg                                                                                                                                             >  1:1926637/1‑119 (MQ=18)
                                             aGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTATCACATGACCTCCAGGTCTGGTGATTCCtgtg                                                                                                                                           <  2:3538539/118‑1 (MQ=18)
                                               ttCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACCGGAAGCACCCGACCGGGTGGTCCAGTGCTACCGGGGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAggcgg                                                                                                         >  2:6285763/1‑150 (MQ=25)
                                                                        caGGCCTGACAGCGGGGCTGCTTTGCCGCCCTGCGCGGGCCCGCCCGCTCCCACCGGAATCCCCCGCCGGGGTGGTCATGTGATACCTGTGGGCGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGAt                                                                               <  1:7896219/149‑1 (MQ=25)
                                                                            ccTCAAATCGGGCCTGCTTTCCACCCCTACGCGGCCACCCCCGCTCCCACAGGAATCACCACACCTGGAGGACATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTg                                                                           <  2:6214021/150‑1 (MQ=37)
                                                                              tGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCAt                                                                                                                                                       >  1:7132011/1‑73 (MQ=255)
                                                                              tGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACACGAAAAACCAGACCGGGAGGTCCTGTGTTACCGGTGGGAGCGGGCGTGCCCGCGCAGGGCCCCGAGGCGGCCCC‑AATTCAAATTCCGGCGTTGATACAGGt                                                                         >  2:2274308/1‑150 (MQ=25)
                                                                                aaaTCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTAGTcc                                                                       <  2:5831914/150‑1 (MQ=255)
                                                                                 aaTCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCCCCCCCAGGAATCACCAGACCGGGTGGTCATGTGATACCTGTGGGCGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCa                                                                      <  2:1739102/150‑1 (MQ=38)
                                                                                 aaTCGGGGCGGCTTTGCAGCCCTGCGCGGGCCCGCCCGCTCCCACCCGAAACACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCa                                                                      <  1:714473/150‑1 (MQ=37)
                                                                                                       ttCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCg                                                <  2:7165282/150‑1 (MQ=255)
                                                                                                                acacccGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGcc                                       >  1:1190794/4‑150 (MQ=255)
                                                                                                                aCGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGcc                                       >  2:7352918/1‑150 (MQ=255)
                                                                                                                  gCCCGCTCCCACAGGTATCACATGACCTCCAGGTCTGGTGATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCgg                                     >  2:6536687/1‑150 (MQ=21)
                                                                                                                  gCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCgg                                     >  2:3080627/1‑150 (MQ=255)
                                                                                                                    ccGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTcc                                                                                        <  2:6061562/97‑1 (MQ=255)
                                                                                                                    ccGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTcc                                                                                        >  1:6061562/1‑97 (MQ=255)
                                                                                                                        tCCCACAGGTATCACATGACCTCCAGGTCTGGTGATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGc                               >  1:5933150/1‑150 (MQ=21)
                                                                                                                        tCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATCTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGc                               >  2:3272345/1‑150 (MQ=255)
                                                                                                                          ccACAGGTATCACATGACCTCCAGGTCTGGTGATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTcc                                                                       <  1:7682580/108‑1 (MQ=14)
                                                                                                                          ccACAGGTATCACATGACCTCCAGGTCTGGTGATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCt                             >  1:3596792/1‑150 (MQ=25)
                                                                                                                          ccACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTcgcg                                                              <  1:7322284/117‑1 (MQ=255)
                                                                                                                          ccACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCt                             >  2:1041316/1‑150 (MQ=255)
                                                                                                                          ccACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCt                             >  1:6359065/1‑150 (MQ=255)
                                                                                                                          ccACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCt                             >  1:3519355/1‑150 (MQ=255)
                                                                                                                           cacaGGTATCACATGACCTCCAGGTCTGGTGATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTc                            >  1:1887838/1‑150 (MQ=25)
                                                                                                                            acaGGTATCACATGACCTCCAGGTCTGGTGATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTcc                           >  2:6988003/1‑150 (MQ=25)
                                                                                                                            acaGGTATCACATGACCTCCAGGTCTGGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTcc                           >  1:4549047/1‑150 (MQ=25)
                                                                                                                            acaGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTcc                           >  2:3821535/1‑150 (MQ=255)
                                                                                                                             caGGTATCACATGACCTCCAGGTCTGGTGATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATa                                                                              <  2:3686690/98‑1 (MQ=14)
                                                                                                                             caGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCa                          >  2:2804929/1‑150 (MQ=255)
                                                                                                                             caGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCa                          >  2:5219035/1‑150 (MQ=255)
                                                                                                                              aGGTATCACATGACCTCCAGGTCTGGTGATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTcgcg                                                              <  2:7423444/113‑1 (MQ=17)
                                                                                                                              aGGTATCACATGACCTCCAGGTCTGGTGATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCg                                      <  2:762618/137‑1 (MQ=21)
                                                                                                                              aGGTATCACATGACCTCCAGGTCTGGTGATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGGCCAGATTcgcg                                                              >  1:2986241/1‑113 (MQ=17)
                                                                                                                              aGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCTGCAAAGCAGcc                                                                                                       <  1:679918/73‑1 (MQ=17)
                                                                                                                              aGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAg                         >  1:968708/1‑150 (MQ=255)
                                                                                                                               ggtaTCACATGACCTCCAGGTCTGGTGATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAgc                        >  2:2335587/4‑150 (MQ=25)
                                                                                                                                gtaTCACATGACCTCCAGGTCTGGTGATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAgcg                       >  2:7597493/3‑150 (MQ=25)
                                                                                                                                gAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAgcg                       >  2:6635472/1‑150 (MQ=255)
                                                                                                                                  aTCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCa                     >  1:5158014/1‑150 (MQ=255)
                                                                                                                                   tcacatgACCTCCAGGTC‑TGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATa                   >  1:5286297/7‑150 (MQ=38)
                                                                                                                                    cACCAGCCCTGGTGGTCATGTGATACCTGGGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATa                   <  2:4590971/150‑1 (MQ=255)
                                                                                                                                    cACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATa                   <  2:5177971/150‑1 (MQ=255)
                                                                                                                                     acatgACCTCCAGGTCTGGTGATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCTGCAAAGCAGCCCCGATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCAt                                  >  1:2117724/5‑135 (MQ=17)
                                                                                                                                     acatgACCTCCAGGTCTGGTGATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATAc                  >  2:5466902/5‑150 (MQ=37)
                                                                                                                                     acatgACCTCCAGGTCTGGTGATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATAc                  >  2:8339838/5‑150 (MQ=37)
                                                                                                                                     acatgACCTCCAGGTCTGGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATAc                  >  2:2624733/5‑150 (MQ=38)
                                                                                                                                     aCCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATAc                  >  2:6131752/1‑150 (MQ=255)
                                                                                                                                     aCCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATAc                  >  1:8119229/1‑150 (MQ=255)
                                                                                                                                      ccAGACCTGGAGGTCATGTGATACCTGGGTGAGCGGGCGTGCCATCCAAGGCCAGCTCGGCGGCCCC‑AATTCAATATCCGGCTTAGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCAGGCATCGCCTCAACCGCATACa                 >  2:2942695/1‑150 (MQ=255)
                                                                                                                                      catgACCTCCAGGTCTGGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACa                 >  2:1315/4‑150 (MQ=38)
                                                                                                                                       cAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAg                >  1:5670886/1‑150 (MQ=255)
                                                                                                                                       atgACCTCCAGGTCTGGTGATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAg                >  1:8528541/3‑150 (MQ=37)
                                                                                                                                       atgACCTCCAGGTCTGGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGACTACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATAccg                >  2:6920746/3‑148 (MQ=37)
                                                                                                                                        aGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAGc               >  2:5705317/1‑150 (MQ=255)
                                                                                                                                        aGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAGc               >  2:5083495/1‑150 (MQ=255)
                                                                                                                                           cctccaGGTCTGGTGATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAGCTTg            >  2:7252183/6‑150 (MQ=255)
                                                                                                                                            cTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAGCTTGc           >  2:8815798/1‑150 (MQ=255)
                                                                                                                                              ggAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAGCTTGccc         >  1:2009046/1‑150 (MQ=255)
                                                                                                                                                   tCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTc                                          >  1:1172228/1‑112 (MQ=255)
                                                                                                                                                   tCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTc                                          <  2:1172228/112‑1 (MQ=255)
                                                                                                                                                   tCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAGCTTGCCCATCAc    >  1:6624266/1‑150 (MQ=255)
                                                                                                                                                     aTGTGATACCTTTGGGAGCGGCCGAGCCCGCGAAGGCCCGCTAGGCGGCCCC‑AGTTCAATTTCCGGCTTAGAGACTGGTCCAGATTCGCGAACGAATACGGTCGATCTTCGCCGGCAGCGCCTCCAGCGCAGACAGCTTGCCCATCAccg  >  2:8674268/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
CCCGGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCC‑ATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAGCTTGCCCATCACCT  >  NC_002947/1018051‑1018349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑