BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	1,013,189	C→G	C170S (TGC→TCC)	SBRG_Ecoli_galK ←	SBRG_Ecoli_galK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,013,189	0	C	G	98.5%	1209.9 / 3.9	333	C170S (TGC→TCC)	SBRG_Ecoli_galK	SBRG_Ecoli_galK
Reads supporting (aligned to +/- strand): ref base C (3/2); new base G (162/166); total (165/168)
Fisher's exact test for biased strand distribution p-value = 6.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

Only 100 of 333 total aligned reads displayed.

CAGGGTACGTTTGAAGTTACTGTTGATGATGACGACAGCCACACCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTCCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGG  >  NC_002947/1013044‑1013334

 aGGGTACGTTTGAAGTTACTGTTGATGATGACGACAGCCACACCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGtt                                                                                                                                               >  2:5994692/1‑149 (MQ=255)

  gggTACGTTTGAAGTTACTGTTGATGATGACGACAGCCACACCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTa                                                                                                                                              >  1:7645101/1‑149 (MQ=255)

    gTACGTTTGAAGTTACTGTTGATGATGACGACAGCCACACCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACa                                                                                                                                            <  2:2464529/149‑1 (MQ=255)

    gTACGTTTGAAGTTACTGTTGATGATGACGACAGCCACACCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACa                                                                                                                                            <  2:7270302/149‑1 (MQ=255)

     tACGTTTGAAGTTACTGTTGATGATGACGACAGCCACACCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTACTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAg                                                                                                                                           <  1:7775002/149‑1 (MQ=255)

       cGTTTGAAGTTACTGTTGATGATGCCGACAGCCTCACCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGcc                                                                                                                                         <  2:1422113/149‑1 (MQ=255)

          ttGAAGTTACTGTTGATGATGACGACAGCCACACCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTAc                                                                                                                                      >  1:7534955/1‑149 (MQ=255)

          ttGAAGTTACTGTAGATGATGACGACAGCCACACCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGATTTACAGCCTAc                                                                                                                                      >  2:5291417/1‑149 (MQ=255)

             aaGTTACTGTTGATGATGACGACAGCCACACCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACaaa                                                                                                                                   <  1:2185232/149‑1 (MQ=255)

             aaGTTACTGTTGATGATGACGACAGCCACACCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACaaa                                                                                                                                   <  2:7763823/149‑1 (MQ=255)

               gTTACTGTTGATGATGACGACAGCCACACCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACt                                                                                                                                 <  1:1051883/149‑1 (MQ=255)

               gTTACTGTTGATGATGACGACAGCCACACCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACt                                                                                                                                 <  1:6578062/149‑1 (MQ=255)

               gTTACTGTTGATGATGACGACAGCCACACCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACt                                                                                                                                 <  1:6271429/149‑1 (MQ=255)

                 tACTGTTGATGATGACGACAGCCACACCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACTgg                                                                                                                               >  1:6399480/1‑149 (MQ=255)

                   cTGTTGATGATGACGACAGCCACACCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACTGGtt                                                                                                                             <  2:5876305/149‑1 (MQ=255)

                   cTGTTGATGATGACGACAGCCACACCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACTGGtt                                                                                                                             >  1:3478120/1‑149 (MQ=255)

                   cTGTTGATGATGACGACAGCCACACCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACTGGtt                                                                                                                             >  2:887352/1‑149 (MQ=255)

                       tgatgatgaCGACAGCCACACCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACACACTGGTTTTCt                                                                                                                         >  2:5075822/1‑149 (MQ=255)

                       tgatgatgaCGACAGCCACACCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCt                                                                                                                         >  1:2617672/1‑149 (MQ=255)

                          tgatgaCGACAGCCACACCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCt                                                                                                                      <  1:1345498/149‑1 (MQ=255)

                           gatgaCGACAGCCACACCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCtt                                                                                                                     <  2:2772868/149‑1 (MQ=255)

                           gatgaCGACAGCCACACCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCtt                                                                                                                     <  1:806554/149‑1 (MQ=255)

                            atgaCGACAGCCACACCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCTTc                                                                                                                    <  1:8148719/149‑1 (MQ=255)

                               acgacAGCCACACCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTg                                                                                                                 >  2:4210837/1‑149 (MQ=255)

                                cgacAGCCACACCTTTGGGGAGGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCGGATCCATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGa                                                                                                                <  2:7905279/149‑1 (MQ=255)

                                 gacAGCCACACCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGAc                                                                                                               <  1:7225881/149‑1 (MQ=255)

                                   cAGCCACACCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCg                                                                                                             <  2:7709825/149‑1 (MQ=255)

                                    aGCCACACCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGt                                                                                                            <  2:1232936/149‑1 (MQ=255)

                                    aGCCACACCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGt                                                                                                            <  2:1259937/149‑1 (MQ=255)

                                        acacCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCATACTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAg                                                                                                        >  1:8223477/1‑149 (MQ=255)

                                          acCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAgcg                                                                                                      >  1:6845031/1‑149 (MQ=255)

                                           ccTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAgcgc                                                                                                     >  1:8673199/1‑149 (MQ=255)

                                            cTTTGGGCATGGAAACTTCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGcgcg                                                                                                    >  1:6903365/1‑149 (MQ=255)

                                               tGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGAtt                                                                                                 >  1:1418155/1‑149 (MQ=255)

                                                   cATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTtgtg                                                                                             >  1:1760403/1‑149 (MQ=255)

                                                     tGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTgcg                                                                                           >  2:8052491/1‑149 (MQ=255)

                                                     tGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTgcg                                                                                           <  2:1593999/149‑1 (MQ=255)

                                                       gAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGcc                                                                                         <  2:2037851/149‑1 (MQ=255)

                                                        aaaCTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCg                                                                                        >  1:7682724/1‑149 (MQ=255)

                                                           cTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTcc                                                                                     <  1:5752924/149‑1 (MQ=255)

                                                           cTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTcc                                                                                     >  1:3839449/1‑149 (MQ=255)

                                                             gCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAg                                                                                   <  1:5084592/149‑1 (MQ=255)

                                                                ttGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCgg                                                                                >  1:2795568/1‑149 (MQ=255)

                                                                  ggTCCCCCGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCa                                                                              >  1:297788/1‑149 (MQ=255)

                                                                    tCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAga                                                                            >  1:7311345/1‑149 (MQ=255)

                                                                    tCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAga                                                                            <  1:7854570/149‑1 (MQ=255)

                                                                    tCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAga                                                                            <  2:6014547/149‑1 (MQ=255)

                                                                     ccccAGTGAGCGGCAATCTATCAGAAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGATGATACATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCTTCATGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAgat                                                                           >  2:5519854/1‑149 (MQ=255)

                                                                     ccccAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAgat                                                                           >  2:674095/1‑149 (MQ=255)

                                                                      cccAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAgatg                                                                          <  1:5407322/149‑1 (MQ=255)

                                                                       ccAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAgatga                                                                         <  1:5992029/149‑1 (MQ=255)

                                                                        cAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAgatgat                                                                        <  2:2191342/149‑1 (MQ=255)

                                                                          gTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATaa                                                                      <  2:8459969/149‑1 (MQ=255)

                                                                          gTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATaa                                                                      <  1:5291417/149‑1 (MQ=255)

                                                                           tGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATaaa                                                                     <  2:6253951/149‑1 (MQ=255)

                                                                           tGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATaaa                                                                     <  1:4261237/149‑1 (MQ=255)

                                                                            gAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAg                                                                    >  1:466585/1‑149 (MQ=255)

                                                                              gCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAgct                                                                  <  1:667899/149‑1 (MQ=255)

                                                                               cGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAgctg                                                                 <  1:439805/149‑1 (MQ=255)

                                                                                ggCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAgctgc                                                                <  1:2941606/149‑1 (MQ=255)

                                                                                  cAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGctgctg                                                              >  1:8489932/1‑149 (MQ=255)

                                                                                  cAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGctgctg                                                              >  2:3068878/1‑149 (MQ=255)

                                                                                   aaTCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCtgctgc                                                             >  2:8228604/1‑149 (MQ=255)

                                                                                    aTCGATCAGCATGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCa                                                            <  2:9056352/149‑1 (MQ=255)

                                                                                      cGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAAt                                                          <  1:6470383/149‑1 (MQ=255)

                                                                                        aTCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATAc                                                        <  2:1806484/149‑1 (MQ=255)

                                                                                         tCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACg                                                       >  2:5501197/1‑149 (MQ=255)

                                                                                           aGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGt                                                     <  1:3272355/149‑1 (MQ=255)

                                                                                             cAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTc                                                   >  1:329744/1‑149 (MQ=255)

                                                                                             cAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTc                                                   >  1:946822/1‑149 (MQ=255)

                                                                                              aaGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTcc                                                  >  2:259884/1‑149 (MQ=255)

                                                                                                  cATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGAcc                                              <  2:4403524/149‑1 (MQ=255)

                                                                                                   aTGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACcg                                             <  2:6566041/149‑1 (MQ=255)

                                                                                                   aTGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACcg                                             >  1:5115922/1‑149 (MQ=255)

                                                                                                    tGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACcgc                                            >  2:6931345/1‑149 (MQ=255)

                                                                                                     gATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACcgcg                                           >  1:5671276/1‑149 (MQ=255)

                                                                                                      aTCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGa                                          <  1:3008798/149‑1 (MQ=255)

                                                                                                          ttcttGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTc                                      >  2:6386624/1‑149 (MQ=255)

                                                                                                           tcttGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTcc                                     <  1:1289129/149‑1 (MQ=255)

                                                                                                           tcttGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTcc                                     <  2:4048754/149‑1 (MQ=255)

                                                                                                           tcttGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTcc                                     <  2:3222845/149‑1 (MQ=255)

                                                                                                             ttGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTCCAg                                   <  2:3000343/149‑1 (MQ=255)

                                                                                                                ccGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTCCAGTGa                                <  2:7575241/149‑1 (MQ=255)

                                                                                                                 cGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTCCAGTGaa                               >  1:2383624/1‑149 (MQ=255)

                                                                                                                 cGAGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTCCAGTGaa                               <  1:7708870/149‑1 (MQ=255)

                                                                                                                   aGCGCGGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTCCAGTGAAGc                             >  2:1568451/1‑149 (MQ=255)

                                                                                                                       cgGAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTCCAGTGAAGCGgaa                         >  2:8929918/1‑149 (MQ=255)

                                                                                                                        ggAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTCCAGTGAAGCGgacg                        >  2:5636877/1‑147 (MQ=255)

                                                                                                                        ggAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTCCAGTGAAGCGgaag                        >  1:1906771/1‑149 (MQ=255)

                                                                                                                         gAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTCCAGTGAAGCGgaaga                       <  2:3771083/149‑1 (MQ=255)

                                                                                                                         gAAATTAGCTGATCCATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTCCAGTGAAGCGgaaga                       <  1:4974229/149‑1 (MQ=255)

                                                                                                                                 cTGATCCATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTCCAGTGAAGCGGAAGAACTTAAcc               <  2:6183903/149‑1 (MQ=255)

                                                                                                                                   gATCCATGATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTCCAGTGAAGCGGAAGAACTTAACCCg             >  2:4365442/1‑149 (MQ=255)

                                                                                                                                     tCCATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTCCAGTGAAGCGGAAGAACTTAACCCGGc           >  2:1157384/1‑149 (MQ=255)

                                                                                                                                     tCCATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTCCAGTGAAGCGGAAGAACTTAACCCGGc           >  2:8648220/1‑149 (MQ=255)

                                                                                                                                     tCCATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTCCAGTGAAGCGGAAGAACTTAACCCGGc           >  2:4863199/1‑149 (MQ=255)

                                                                                                                                       cATGATCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTCCAGTGAAGCGGAAGAACTTAACCCGGCAc         <  1:674192/149‑1 (MQ=255)

                                                                                                                                           aTCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTCCAGTGAAGCGGAAGAACTTAACCCGGCACCCTg     >  1:9065142/1‑149 (MQ=255)

                                                                                                                                            tCCCGGAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTCCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGc    <  1:5498417/149‑1 (MQ=255)

                                                                                                                                             cccGGAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTCCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCg   <  2:4539377/149‑1 (MQ=255)

CAGGGTACGTTTGAAGTTACTGTTGATGATGACGACAGCCACACCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTCCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGG  >  NC_002947/1013044‑1013334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`