Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 3,827,929 C→A V28F (GTC→TTC)  ptxS ← 2‑ketogluconate utilization repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,827,9290CA99.5% 2979.4 / NA 848V28F (GTC→TTC) ptxS2‑ketogluconate utilization repressor
Reads supporting (aligned to +/- strand):  ref base C (3/1);  new base A (392/452);  total (395/453)
Fisher's exact test for biased strand distribution p-value = 3.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 848 total aligned reads displayed.

CCAGCATGCCGATCAGTCGCGTCTGGCCGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGTGATGAAACCGGTTTCAATACAGCAAAAATGCGCGGGT  >  NC_002947/3827782‑3828075
                                                                                                                                                   |                                                                                                                                                  
 caGCATGCCGATCAGTCGCGTCTGGCCGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGt                                                                                                                                                 <  1:7031490/150‑1 (MQ=255)
  aGCATGCCGATCAGTCGCGTCTGGCCGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTg                                                                                                                                                >  2:10408033/1‑150 (MQ=255)
       gCCGATCAGTCGCGTCTGGCCGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCttt                                                                                                                                           <  1:13573191/150‑1 (MQ=255)
       gCCGATCAGTCGCGTCTGGCCGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCttt                                                                                                                                           <  1:5909754/150‑1 (MQ=255)
         cGATCAGTCGCGTCTGGCCGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTgg                                                                                                                                         <  1:13262293/150‑1 (MQ=255)
            tCAGTCGCGTCTGGCCGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGaaa                                                                                                                                      <  1:784175/150‑1 (MQ=255)
                 cgcgTCTGGCCGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCa                                                                                                                                 <  2:11010942/150‑1 (MQ=255)
                  gcgTCTGGCCGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAg                                                                                                                                <  2:1732757/150‑1 (MQ=255)
                      cTGGCCGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAac                                                                                                                            >  1:3476242/1‑150 (MQ=255)
                        ggCAGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAacac                                                                                                                          >  1:1859618/1‑150 (MQ=255)
                            gcgcTTTAGGTCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGc                                                                                                                               <  1:4483788/141‑1 (MQ=255)
                              gcTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCa                                                                                                                    <  2:15334569/150‑1 (MQ=255)
                              gcTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCa                                                                                                                    >  1:14828692/1‑150 (MQ=255)
                                tttAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACt                                                                                                                  >  1:8879198/1‑150 (MQ=255)
                                   aGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCa                                                                                                               <  2:13310845/150‑1 (MQ=255)
                                   aGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCa                                                                                                               >  2:4541139/1‑150 (MQ=255)
                                    ggCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATTACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCAc                                                                                                              >  1:11491029/1‑150 (MQ=255)
                                     gCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACt                                                                                                             >  1:12676517/1‑150 (MQ=255)
                                     gCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACt                                                                                                             >  2:3521280/1‑150 (MQ=255)
                                     gCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACt                                                                                                             <  1:16836727/150‑1 (MQ=255)
                                       cgcgAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGa                                                                                                           >  2:17882181/1‑150 (MQ=255)
                                        gcgAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGAt                                                                                                          <  1:10001338/150‑1 (MQ=255)
                                        gcgAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGAt                                                                                                          <  1:14327480/150‑1 (MQ=255)
                                         cgAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATg                                                                                                         <  2:5025562/150‑1 (MQ=255)
                                              cATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGAc                                                                                                    <  1:15226645/150‑1 (MQ=255)
                                              cATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGAc                                                                                                    >  2:5343283/1‑150 (MQ=255)
                                               aTCTGGTTGGGGCGATAGCCGAGGCGTTCGATTACCTCTTACAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTTGAAACGCCAGCAAAACGCGCCACTTCACTGATGGTGAcc                                                                                                   >  1:6402455/1‑150 (MQ=255)
                                               aTCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGAGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGAcc                                                                                                   >  1:11817120/1‑150 (MQ=255)
                                                tCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGAccc                                                                                                  >  2:12761078/1‑150 (MQ=255)
                                                         ggCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCggg                                                                                         >  1:17065817/1‑150 (MQ=255)
                                                         ggCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCggg                                                                                         <  2:11620204/150‑1 (MQ=255)
                                                            gATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTc                                                                                              <  1:10714475/142‑1 (MQ=255)
                                                            gATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTAt                                                                                      >  1:3271428/1‑150 (MQ=255)
                                                             aTAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATg                                                                                     >  1:4945377/1‑150 (MQ=255)
                                                              tAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATgg                                                                                    <  2:10742745/150‑1 (MQ=255)
                                                                gCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGc                                                                                  >  1:13900779/1‑150 (MQ=255)
                                                                 ccGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGAc                                                                                                    <  1:18299154/131‑1 (MQ=255)
                                                                  cGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAg                                                                                <  2:8064601/150‑1 (MQ=255)
                                                                   gAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAgg                                                                               >  2:7919189/1‑150 (MQ=255)
                                                                      gCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGgcgc                                                                            <  2:12203283/150‑1 (MQ=255)
                                                                           cGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCgg                                                                       >  1:2740294/1‑150 (MQ=255)
                                                                               gACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCAt                                                                   <  2:8841464/150‑1 (MQ=255)
                                                                               gACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCAt                                                                   <  2:18499279/150‑1 (MQ=255)
                                                                                 ccTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGGTCGCGGGTATGGGCAGGCGCGTCGGTCATgg                                                                 <  1:522783/150‑1 (MQ=255)
                                                                                  ctctTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATggg                                                                >  1:9966261/1‑150 (MQ=255)
                                                                                  ctctTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATggg                                                                >  1:12914737/1‑150 (MQ=255)
                                                                                   tctTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGt                                                               >  1:17540341/1‑150 (MQ=255)
                                                                                    ctTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCgg                                                                                          <  2:14228870/122‑1 (MQ=255)
                                                                                     ttCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGttt                                                             >  1:2174707/1‑150 (MQ=255)
                                                                                       ccAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTc                                                           <  2:16575088/150‑1 (MQ=255)
                                                                                        cAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGTGCAGGCGCGTCGGTCATGGGTTTTcc                                                          >  1:10274968/1‑150 (MQ=255)
                                                                                        cAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTcc                                                          <  2:6474169/150‑1 (MQ=255)
                                                                                        cAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTcc                                                          <  2:2395990/150‑1 (MQ=255)
                                                                                           ccGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTa                                                       <  1:9417790/150‑1 (MQ=255)
                                                                                           ccGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTa                                                       <  1:7290411/150‑1 (MQ=255)
                                                                                            cGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCgg                                                                       <  2:3167790/133‑1 (MQ=255)
                                                                                             gCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATg                                                     >  1:18092303/1‑150 (MQ=255)
                                                                                               ttGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGAt                                                   >  2:7230168/1‑150 (MQ=255)
                                                                                                tGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGAtt                                                  <  2:18092303/150‑1 (MQ=255)
                                                                                                tGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGAtt                                                  <  2:6970935/150‑1 (MQ=255)
                                                                                                     gTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGtttt                                             >  2:11937036/1‑150 (MQ=255)
                                                                                                     gTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGtttt                                             <  2:7607889/150‑1 (MQ=255)
                                                                                                      tGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTa                                            <  2:9060012/150‑1 (MQ=255)
                                                                                                      tGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTa                                            <  2:6151739/150‑1 (MQ=255)
                                                                                                      tGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTa                                            <  2:15025348/150‑1 (MQ=255)
                                                                                                       ggCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTaa                                           <  1:10356639/150‑1 (MQ=255)
                                                                                                       ggCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTaa                                           <  1:17691557/150‑1 (MQ=255)
                                                                                                         cTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATa                                         <  1:9146879/150‑1 (MQ=255)
                                                                                                          ttCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAg                                        >  1:15072257/1‑150 (MQ=255)
                                                                                                           tCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGt                                       <  2:13701854/150‑1 (MQ=255)
                                                                                                           tCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGt                                       <  2:4113367/150‑1 (MQ=255)
                                                                                                            cGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGt                                       <  2:14570650/149‑1 (MQ=255)
                                                                                                             ggCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGtga                                     >  1:4670849/1‑150 (MQ=255)
                                                                                                              gCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGtgat                                    <  2:10259266/150‑1 (MQ=255)
                                                                                                              gCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGtgat                                    >  1:16269231/1‑150 (MQ=255)
                                                                                                                cAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGtgatga                                  <  1:8002381/150‑1 (MQ=255)
                                                                                                                  gCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGt                                       >  2:2148619/1‑143 (MQ=255)
                                                                                                                     aTTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGTGATGAAACCg                             >  2:16186825/1‑150 (MQ=255)
                                                                                                                      ttGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGa                                                    <  2:12608001/126‑1 (MQ=255)
                                                                                                                       tGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGTGATGAAACCGGt                           <  2:17644172/150‑1 (MQ=255)
                                                                                                                         gCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGTGATGAAACCGGttt                         <  1:5633059/150‑1 (MQ=255)
                                                                                                                            gTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGTGATGAAACCGGTTTCaa                      <  1:15539058/150‑1 (MQ=255)
                                                                                                                            gTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGTGATGAAACCGGTTTCaa                      <  2:15688479/150‑1 (MQ=255)
                                                                                                                             tCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGTGATGAAACCGGTTTCAAt                     >  1:3051454/1‑150 (MQ=255)
                                                                                                                               gCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGTGATGAAACCGGTTTCAATAc                   >  2:15943221/1‑150 (MQ=255)
                                                                                                                                ccACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGTGATGAAACCGGTTTCAATACa                  <  2:18328363/150‑1 (MQ=255)
                                                                                                                                ccACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGTGATGAAACCGGTTTCAATACa                  >  2:17053945/1‑150 (MQ=255)
                                                                                                                                ccACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGTGATGAAACCGGTTTCAATACa                  <  2:5667493/150‑1 (MQ=255)
                                                                                                                                ccACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGTGATGAAACCGGTTTCAATACa                  <  1:6672877/150‑1 (MQ=255)
                                                                                                                                ccACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGTGATGAAACCGGTTTCAATACa                  >  1:11015390/1‑150 (MQ=255)
                                                                                                                                  aCCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGTGATGAAACCGGTTTCAATa                    >  1:8426726/1‑146 (MQ=255)
                                                                                                                                  aCCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGTGATGAAACCGGTTTCAATa                    <  2:8426592/146‑1 (MQ=255)
                                                                                                                                      aTGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGtttt                                             <  2:4918310/117‑1 (MQ=255)
                                                                                                                                           aCGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCAt                                                                   >  1:15910356/1‑90 (MQ=255)
                                                                                                                                           aCGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGTGATGAAACCGGTTTCAATACAGCAAAAATgcg       >  1:11245008/1‑150 (MQ=255)
                                                                                                                                           aCGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGTGATGAAACCGGTTTCAATACAGCAAAAATgcg       >  1:10860629/1‑150 (MQ=255)
                                                                                                                                            cGGGAGAAGGTGGCTTTTGAAACGCCAGCATCACGCGCCACTTCACTGATGGTGTCCCGTTCGCGTGTATGGGCATGCGCGTCTGTGATGGGTTTTCCTTATGATTGTTTTAATAGTGATGAAACCGGTTTCAATACAGCAAAAATgcgc      <  2:9395278/150‑1 (MQ=255)
                                                                                                                                              ggAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGTGATGAAACCGGTTTCAATACAGCAAAAATGCGCgg    >  1:4129376/1‑150 (MQ=255)
                                                                                                                                              ggAGAAGGGGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGGATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGAGTGTTTTAATAGTGATGAAACCGGTTTCAATACAGCAAAAATGCGCgg    >  2:4467329/1‑150 (MQ=255)
                                                                                                                                               gagaAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGTGATGAAACCGGTTTCAATACAGCAAAAATGCGCggg   >  2:11378885/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CCAGCATGCCGATCAGTCGCGTCTGGCCGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGTGATGAAACCGGTTTCAATACAGCAAAAATGCGCGGGT  >  NC_002947/3827782‑3828075

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑