Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	4,743,223	A→C	intergenic (+492/+260)	gltA → / ← yeiW	citrate synthase/putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,743,223	0	A	C	100.0%	300.3 / NA	79	intergenic (+492/+260)	gltA/yeiW	citrate synthase/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (39/40);  total (39/40)

TGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑AACCT‑CCGCCAAC‑T‑GCATTCGTGAACCAAGCAACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGA  >  NC_002947/4743099‑4743346                                                                                                                                 |                                                                                                                            tGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTT‑CTGCGACT‑T‑GCATTCGTGAATCCAGGCACAGGc                                                                                                                   >  2:1520709/1‑136 (MQ=255) tGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTT‑CTGCGACT‑T‑GCATTCGTGAATCCAGGCACAGGc                                                                                                                   >  2:1642988/1‑136 (MQ=255) tGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTT‑CTGCGACT‑T‑GCATTCGTGAATCCAGGCACAGGc                                                                                                                   >  2:2636630/1‑136 (MQ=255)        cGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTT‑CTGCGACT‑T‑GCATTCGTGAATCCAGGCACAGGCCCCGGac                                                                                                            <  1:162229/136‑1 (MQ=255)        cGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTT‑CTGCGACT‑T‑GCATTCGTGAATCCAGGCACAGGCCCCGGac                                                                                                            <  1:3174871/136‑1 (MQ=255)           aaTGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTT‑CTGCGACT‑T‑GCATTCGTGAATCCAGGCACAGGCCCCGGACACg                                                                                                         >  2:663830/1‑136 (MQ=255)           aaTGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTT‑CTGCGACT‑T‑GCATTCGTGAATCCAGGCACAGGCCCCGGACACg                                                                                                         >  2:2636705/1‑136 (MQ=255)                  agcaCGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTT‑CTGCGACT‑T‑GCATTCGTGAATCCAGGCACAGGCCCCGGACACGTcctcct                                                                                                  <  2:2309959/136‑1 (MQ=255)                  agcaCGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTT‑CTGCGACT‑T‑GCATTCGTGAATCCAGGCACAGGCCCCGGACACGTcctcct                                                                                                  <  1:2321049/136‑1 (MQ=255)                  agcaCGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGCCACCTTCGTGCTTGAGCAGCTT‑CTGCGACT‑T‑GCATTCGTGAATCCAGGCACAGGCCCCGGACACGTcctcct                                                                                                  <  2:188001/136‑1 (MQ=255)                   gcaCGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTT‑CTGCGACT‑T‑GCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTg                                                                                                 <  2:2017801/136‑1 (MQ=255)                    caCGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCTCCTTCGTGCATGAG‑CAGCT‑TCTGCGAC‑TAGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGc                                                                                                >  1:165036/1‑136 (MQ=255)                    caCGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTT‑CTGCGACT‑T‑GCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGc                                                                                                >  2:2899902/1‑136 (MQ=255)                    caCGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTT‑CTGCGACT‑T‑GCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGc                                                                                                >  2:2502132/1‑136 (MQ=255)                    caCGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTT‑CTGCGACT‑T‑GCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGc                                                                                                >  2:2536902/1‑136 (MQ=255)                    caCGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTT‑CTGCGACT‑T‑GCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGc                                                                                                >  2:2532357/1‑136 (MQ=255)                        gAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTT‑CTGCGACT‑T‑GCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAgcg                                                                                            <  1:1799504/136‑1 (MQ=255)                        gAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTT‑CTGCGACT‑T‑GCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAgcg                                                                                            <  2:1570119/136‑1 (MQ=255)                        gAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTT‑CTGCGACT‑T‑GCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAgcg                                                                                            <  1:2751749/136‑1 (MQ=255)                                 ggcgTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTT‑CTGCGACT‑T‑GCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCt                                                                                   >  2:1256145/1‑136 (MQ=255)                                 ggcgTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTT‑CTGCGACT‑T‑GCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCt                                                                                   >  2:2258339/1‑136 (MQ=255)                                 ggcgTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTT‑CTGCGACT‑T‑GCATTCGTGAATCCAGGCACAGGCCCCGGACACGACCTCCTGCAGCGCCTCGACCt                                                                                   >  2:1936089/1‑136 (MQ=255)                                     tCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTT‑CTGCGACT‑T‑GCATTCGTGAATCCAGGCACAGGCCCCGGACACGTcctcct                                                                                                  <  2:692348/117‑1 (MQ=255)                                     tCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTT‑CTGCGACT‑T‑GCATTCGTGAATCCAGGCACAGGCCCCGGACACGTcctcct                                                                                                  >  1:692348/1‑117 (MQ=255)                                      cACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTT‑CTGCGACT‑T‑GCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGcc                                                                              >  1:1552438/1‑136 (MQ=255)                                       aCCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTT‑CTGCGACT‑T‑GCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCa                                                                             <  1:2258339/136‑1 (MQ=255)                                        ccGGCCATGGCGGATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTT‑CTGCGACT‑T‑GCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAg                                                                            <  2:2339829/136‑1 (MQ=255)                                            ccATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTT‑CTGCGACT‑T‑GCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAgcggc                                                                        <  2:1160003/136‑1 (MQ=255)                                                 gCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTT‑CTGCGACT‑T‑GCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCtt                                                                   <  1:1110025/136‑1 (MQ=255)                                                 gCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTT‑CTGCGACT‑T‑GCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCtt                                                                   <  1:2899902/136‑1 (MQ=255)                                                  cGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTT‑CTGCGACT‑T‑GCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTg                                                                  >  2:2837116/1‑136 (MQ=255)                                                       tGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTT‑CTGCGACT‑T‑GCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCAc                                                             <  1:2291371/136‑1 (MQ=255)                                                        gAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTT‑CTGCGACT‑T‑GCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACg                                                            <  1:1926257/136‑1 (MQ=255)                                                           cTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTT‑CTGCGACT‑T‑GCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTc                                                         <  1:2536902/136‑1 (MQ=255)                                                           cTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTT‑CTGCGACT‑T‑GCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTc                                                         <  1:2844940/136‑1 (MQ=255)                                                           cTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTT‑CTGCGACT‑T‑GCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTc                                                         <  1:2636705/136‑1 (MQ=255)                                                            tGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTT‑CTGCGACT‑T‑GCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTca                                                        <  1:92446/136‑1 (MQ=255)                                                              ggTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTT‑CTGCGACT‑T‑GCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTcaca                                                      >  2:2542789/1‑136 (MQ=255)                                                              ggTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTT‑CTGCGACT‑T‑GCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTcaca                                                      >  2:232524/1‑136 (MQ=255)                                                                 ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTT‑CTGCGACT‑T‑GCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcg                                                   >  2:1726775/1‑136 (MQ=255)                                                                 ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTT‑CTGCGACT‑T‑GCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcg                                                   >  1:3001871/1‑136 (MQ=255)                                                                  taGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTT‑CTGCGACT‑T‑GCATTCGTGAATCCAGGCACAGGCCGCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                                                  <  1:1325990/136‑1 (MQ=255)                                                                  taGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTT‑CTGCGACT‑T‑GCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                                                  <  1:2112948/136‑1 (MQ=255)                                                                  taGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTT‑CTGCGACT‑T‑GCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                                                  <  1:1474112/136‑1 (MQ=255)                                                                  taGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTT‑CTGCGACT‑T‑GCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                                                  <  1:597932/136‑1 (MQ=255)                                                                  taGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTT‑CTGCGACT‑T‑GCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                                                  <  1:2914977/136‑1 (MQ=255)                                                                  taGGTCCAGCTCTGGTCACCTTCGTGCTGGAG‑CAGCTGCTGCGACT‑T‑GCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCGCGGTCACACcgc                                                  <  2:3155098/136‑1 (MQ=255)                                                                   aGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTT‑CTGCGACT‑T‑GCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgcg                                                 >  1:267622/1‑136 (MQ=255)                                                                    ggTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTT‑CTGCGACT‑T‑GCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGa                                                <  2:1536886/136‑1 (MQ=255)                                                                     gTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTT‑CTGCGACT‑T‑GCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGAt                                               <  1:1330973/136‑1 (MQ=255)                                                                      tCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTT‑CTGCGACT‑T‑GCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATa                                              >  2:1197296/1‑136 (MQ=255)                                                                        cAGCTCTGGTCACCTTCGTGCTTGAGCAGCTT‑CTGCGACT‑T‑GCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAgg                                            <  1:2502132/136‑1 (MQ=255)                                                                        cAGCTCTGGTCACCTTCGTGCTTGAGCAGCTT‑CTGCGACT‑T‑GCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAgg                                            <  1:2532357/136‑1 (MQ=255)                                                                           ctctGGTCACCTTCGTGCTTGAGCAGCTT‑CTGCGACT‑T‑GCATTCGTGAATCCAGGCACAGGCCCCGGACACg                                                                                                         >  1:1634587/1‑72 (MQ=255)                                                                           ctctGGTCACCTTCGTGCTTGAGCAGCTT‑CTGCGACT‑T‑GCATTCGTGAATCCAGGCACAGGCCCCGGACACg                                                                                                         <  2:1634587/72‑1 (MQ=255)                                                                           ctctGGTCACCTTCGTGCTTGAGCAGCTT‑CTGCGACT‑T‑GCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGCTAGGccg                                         >  1:2254782/1‑135 (MQ=255)                                                                           ctctGGTCACCTTCGTGCTTGAGCAGCTT‑CTGCGACT‑T‑GCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCt                                         >  1:785693/1‑136 (MQ=255)                                                                           ctctGGTCACCTTCGTGCTTGAGCAGCTT‑CTGCGACT‑T‑GCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCt                                         >  2:1251288/1‑136 (MQ=255)                                                                           ctctGGTCACCTTCGTGCTTGAGCAGCTT‑CTGCGACT‑T‑GCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTCGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCt                                         >  2:1961167/1‑136 (MQ=255)                                                                           ctctGGTCACCTTCGTGCTTGAGCAGCTT‑CTGCGACT‑T‑GCATTCGTGAATCCAGGCACAGGCCCCGGACACGGCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCt                                         >  2:2891306/1‑136 (MQ=255)                                                                            tctGGTCACCTTCGTGCTTGAGCAGCTT‑CTGCGACT‑T‑GCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGcc                                          >  1:3030119/1‑134 (MQ=255)                                                                            tctGGTCACCTTCGTGCTTGAGCAGCTT‑CTGCGACT‑T‑GCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGcc                                          <  2:3030119/134‑1 (MQ=255)                                                                            tctGGTCACCTTCGTGCTTGAGCAGCTT‑CTGCGACT‑T‑GCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCtt                                        >  2:356474/1‑136 (MQ=255)                                                                             ctGGTCACCTTCGTGCTTGAGCAGCTT‑CTGCGACT‑T‑GCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGa                                                                 >  1:354394/1‑110 (MQ=255)                                                                             ctGGTCACCTTCGTGCTTGAGCAGCTT‑CTGCGACT‑T‑GCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGa                                                                 <  2:354394/110‑1 (MQ=255)                                                                             ctGGTCACCTTCGTGCTTGAGCAGCTT‑CTGCGACT‑T‑GCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTg                                       >  1:2970562/1‑136 (MQ=255)                                                                             ctGGTCACCTTCGTGCTTGAGCAGCTT‑CTGCGACT‑T‑GCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTg                                       >  2:640099/1‑136 (MQ=255)                                                                                   aCCTTCGTGCTTGAGCAGCTT‑CTGCGACT‑T‑GCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGAt                                               >  2:823833/1‑122 (MQ=255)                                                                                   aCCTTCGTGCTTGAGCAGCTT‑CTGCGACT‑T‑GCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGAt                                               <  1:823833/122‑1 (MQ=255)                                                                                   aCCTTCGTGCTTGAGCAGCTT‑CTGCGACT‑T‑GCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTg                                 >  2:53108/1‑136 (MQ=255)                                                                                        cGTGCTTGAG‑CAGCT‑TCTGCGACTT‑GCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGAccc                            >  1:506234/1‑136 (MQ=255)                                                                                        cGTGCTTGAG‑CAGCT‑TCTGCGACTT‑GCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGAccc                            <  1:640099/136‑1 (MQ=255)                                                                                        cGTGCTTGAG‑CAGCT‑TCTGCGACTT‑GCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGAccc                            >  2:1688384/1‑136 (MQ=255)                                                                                                            gcgactt‑GCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGa          >  1:345870/7‑136 (MQ=255)                                                                                                                    gCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTg   <  2:267622/136‑1 (MQ=255)                                                                                                                    gCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTg   <  1:2837116/136‑1 (MQ=255)                                                                                                                     cATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGa  <  1:2542789/136‑1 (MQ=255)                                                                                                                     cATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGa  <  2:506234/136‑1 (MQ=255)                                                                                                                     cATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGa  >  2:692147/1‑136 (MQ=255)                                                                                                                                 |                                                                                                                            TGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑AACCT‑CCGCCAAC‑T‑GCATTCGTGAACCAAGCAACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGA  >  NC_002947/4743099‑4743346

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑