| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 697,103 | T→G | intergenic (+27/‑724) | mmsA‑I → / → PP_16SD | methylmalonate‑semialdehyde dehydrogenase/16S ribosomal RNA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 697,103 | 0 | T | G | 83.3% | 11.1 / ‑5.1 | 13 | intergenic (+27/‑724) | mmsA‑I/PP_16SD | methylmalonate‑semialdehyde dehydrogenase/16S ribosomal RNA |
| Reads supporting (aligned to +/- strand): ref base T (0/1); major base G (10/0); minor base C (2/0); total (12/1) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCCCGGATGGTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGAGGCC‑GTCA‑‑GGC‑C‑TCCCCGC‑TTTGTTTTTGGGGGTTGGTTGTTGCTGTTGGGGAGGAACTGTTTTGAGAGGGTGTTGGCTGTGAGATGTGTCCGCCACAACCCTTGCAGTGCCTGTGAGATCG > NC_002947/696973‑697219 | gCCCGGATGGTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGC‑GGCacgg > 1:1987656/1‑133 (MQ=255) ggTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGC‑GGGA‑‑GGG‑C‑Gcccccg > 1:106793/1‑134 (MQ=255) ataCCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGAGGCC‑GGCA‑‑GGC‑C‑TCCCCGC‑GTTGTTTTTgggg > 2:1235832/1‑136 (MQ=255) aCCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGCG‑GGCG‑‑GGC‑C‑GCCCCCC‑GGTGTTTTTggggg > 1:146006/1‑136 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGCG‑GGCAGCGGC‑C‑CCCCCGG‑TGTGTTTTTggggtggtgg > 1:1187819/1‑130 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGCC‑GGCG‑‑GGC‑G‑CCCCCCC‑GGTGTTTTTTGGgggtggttt > 1:1202372/1‑135 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGC‑GGGA‑‑CGC‑C‑GCCCCCCGTTTTTTTTTTGGggttgtttt > 1:2039618/1‑135 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGCCGGCCG‑‑GGC‑G‑CCCCCCC‑ATGGTTTTTTGGggtgggttt > 1:1604936/1‑135 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGCCGGCCC‑‑GGCGC‑CCCCCGC‑TGT‑TTTTTGGTggttggttt > 1:2442037/1‑135 (MQ=255) aCCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGCC‑GGCG‑‑GCC‑C‑CCCCCCG‑GTGGGTTTTGGGGGTGGGTT‑TTGGTGGTgggg > 1:2739203/1‑136 (MQ=255) cccAGCGTTGGCCGCAGCGGGCCAGCCATGCAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGC‑GGCC‑‑GGC‑CGAGCCCGG‑TTGTTGTTTGGGGGGGGGTTGTTGGGGTGgggg > 2:1507288/1‑136 (MQ=255) cccAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGC‑GGCA‑‑GGG‑C‑GCCCCCC‑GTTGTTTTTTGGGGGTGGTTGTTTGGGTTggggg > 2:1585208/1‑135 (MQ=255) ataTGAAGC‑GGGGAGGCC‑GTCA‑‑GGC‑C‑TCCCCGC‑TTTGTTTTTGGGGGTTGGTTGTTGCTGTTGGGGAGGAACTGTTTTGAGAGGGTGTTGGCTGTGAGATGTGTCCGCCACAACCCTTGCAGTGCCTGTGAGATCg < 2:2130889/134‑1 (MQ=255) | GCCCGGATGGTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGAGGCC‑GTCA‑‑GGC‑C‑TCCCCGC‑TTTGTTTTTGGGGGTTGGTTGTTGCTGTTGGGGAGGAACTGTTTTGAGAGGGTGTTGGCTGTGAGATGTGTCCGCCACAACCCTTGCAGTGCCTGTGAGATCG > NC_002947/696973‑697219 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |