Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 3,824,695 G→C L152V (CTG→GTG)  kguT ← 2‑ketogluconate transporter, putative

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,824,6950GC98.1% 195.5 / ‑3.6 53L152V (CTG→GTG) kguT2‑ketogluconate transporter, putative
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base C (24/28);  total (25/28)
Fisher's exact test for biased strand distribution p-value = 4.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

GGCCGGGCGGTCATCCACCAGGCGCCACCAGAAGAACGCCCAGATCACGGCAGGCAGGCCTTCGATGATGAACATCCAGCGCCAGTCGTAATGCTTGATCAGGTAACCGGACACCACCGACATCCACAGAATGGTCACCGGGTTGCCCAGCATCAGGAAGGTGTTGGCGCGTGAACGCTCGGCCCGGGTGAACCAATGGCACAGGTACACCAGCATGGCCGGCATGACCGCAGCCTCCACCACCCCGAGCAGGAAGCGGATGCC  >  NC_002947/3824567‑3824830
                                                                                                                                |                                                                                                                                       
ggCCGGGCGGTCATCCACCAGGCGCCACCAGAAGAACGCCCAGATCACGGCAGGCAGGCCTTCGATGATGAACATCCAGCGCCAGTCGTAATGCTTGATCAGGTAACCGGACACCACCGACATCCACACAATGGTc                                                                                                                                  >  2:1845167/1‑136 (MQ=255)
   cGGGCGGTCATCCACCAGGCGCCACCAGAAGAACGCCCAGATCACGGCAGGCAGGCCTTCGATGATGAACATCCAGCGCCAGTCGTAATGCTTGATCAGGTAACCGGACACCACCGACATCCACACAATGGTCAcc                                                                                                                               <  1:1125220/136‑1 (MQ=255)
   cGGGCGGTCATCCACCAGGCGCCACCAGAAGAACGCCCAGATCACGGCAGGCAGGCCTTCGATGATGAACATCCAGCGCCAGTCGTAATGCTTGATCAGGTAACCGGACACCACCGACATCCACACAATGGTCAcc                                                                                                                               <  1:221627/136‑1 (MQ=255)
        ggTCATCCACCAGGCGCCACCAGAAGAACGCCCAGATCACGGCAGGCAGGCCTTCGATGATGAACATCCAGCGCCAGTCGTAATGCTTGATCAGGTAACCGGACACCACCGACATCCACACAATGGTCACCGGGtt                                                                                                                          <  1:2775154/136‑1 (MQ=255)
        ggTCATCCACCAGGCGCCACCAGAAGAACGCCCAGATCACGGCAGGCAGGCCTTCGATGATGAACATCCAGCGCCAGTCGTAATGCTTGATCAGGTAACCGGACACCACCGACATCCACACAATGGTCACCGGGtt                                                                                                                          <  1:1705876/136‑1 (MQ=255)
        ggTCATCCACCAGGCGCCACCAGAAGAACGCCCAGATCACGGCAGGCAGGCCTTCGATGATGAACATCCAGCGCCAGTCGTAATGCTTGATCAGGTAACCGGACACCACCGACATCCACACAATGGTCACCGGGtt                                                                                                                          >  2:236044/1‑136 (MQ=255)
          tCATCCACCAGGCGCCACCAGAAGAACGCCCAGATCACGGCAGGCAGGCCTTCGATGATGAACATCCAGCGCCAGTCGTAATGCTTGATCAGGTAACCGGACACCACCGACATCCACACAATGGTCACCGGGTTGc                                                                                                                        <  1:2447949/136‑1 (MQ=255)
          tCATCCACCAGGCGCCACCAGAAGAACGCCCAGATCACGGCAGGCAGGCCTTCGATGATGAACATCCAGCGCCAGTCGTAATGCTTGATCAGGTAACCGGACACCACCGACATCCACACAATGGTCACCGGGTTGc                                                                                                                        <  1:2126146/136‑1 (MQ=255)
           cATCCACCAGGCGCCACCAGAAGAACGCCCAGATCACGGCAGGCAGGCCTTCGATGATGAACATCCAGCGCCAGTCGTAATGCTTGATCAGGTAACCGGACACCACCGACATCCACACAATGGTCACCGGGTTGcc                                                                                                                       <  1:2330081/136‑1 (MQ=255)
            aTCCACCAGGCGCCACCAGAAGAACGCCCAGATCACGGCAGGCAGGCCTTCGATGATGAACATCCAGCGCCAGTCGTAATGCTTGATCAGGTAACCGGACACCACCGACATCCACACAATGGTCACCGGGTTGccc                                                                                                                      <  1:184859/136‑1 (MQ=255)
                   atgagcCACCAGAAGAAAGCCCAGATCTCGGCAGGCAGGCCTTCGATGATGAACATCCAGCTCCAGTCGTATTTCTTGATCAGGTAAACGGACACCTCCGACATCCACACTATGGTCACCGGGTTGCCCAGCATCa                                                                                                               <  1:2140948/132‑1 (MQ=255)
                   aGGCGCCACCAGAAGAACGCCCAGATCACGGCAGGCAGGCCTTCGATGATGAACATCCAGCGCCAGTCGTAATGCTTGATCAGGTAACCGGACACCACCGACATCCACACAATGGTCACCGGGTTGCCCAGCATCa                                                                                                               >  2:1526189/1‑136 (MQ=255)
                   aGGCGCCACCAGAAGAACGCCCAGATCACGGCAGGCAGGCCTTCGATGATGAACATCCAGCGCCAGTCGTAATGCTTGATCAGGTAACCGGACACCACCGACATCCACACAATGGTCACCGGGTTGCCCAGCATCa                                                                                                               <  1:495405/136‑1 (MQ=255)
                   aGGCGCCACCAGAAGAACGCCCAGATCACGGCAGGCAGGCCTTCGATGATGAACATCCAGCGCCAGTCGTAATGCTTGATCAGGTAACCGGACACCACCGACATCCACACAATGGTCACCGGGTTGCCCAGCATCa                                                                                                               <  1:1651117/136‑1 (MQ=255)
                   aGGCGCCACCAGAAGAACGCCCAGATCACGGCAGGCAGGCCTTCGATGATGAACATCCAGCGCCAGTCGTAATGCTTGATCAGGTAACCGGACACCACCGACATCCACACAATGGTCACCGGGTTGCCCAGCATCa                                                                                                               <  1:590868/136‑1 (MQ=255)
                       gcCACCAGAAGAACGCCCAGATCACGGCAGGCAGGCCTTCGATGATGAACATCCAGCGCCAGTCGTAATGCTTGATCAGGTAACCGGACACCACCGACATCCACACAATGGTCACCGGGTTGCCCAGCATCAGGaa                                                                                                           >  1:833768/1‑136 (MQ=255)
                       gcCACCAGAAGAACGCCCAGATCACGGCAGGCAGGCCTTCGAAGATGAACATCCAGCGCCAGTCGTAATGCTTGATCAGGTAACCGCACACCACCGACATCCACACAATGGTCACCGGGATGCCCAGCATCAGGaa                                                                                                           >  1:251390/1‑136 (MQ=255)
                            caGAAGAACGCCCAGATCACGGCAGGCAGGCCTTCGATGATGAACATCCAGCGCCAGTCGTAATGCTTGATCAGGTAACCGGACACCACCGACATCCACACAATGGTCACCGGGTTGCCCAGCATCAGGAAGgtgt                                                                                                      <  1:1744322/136‑1 (MQ=255)
                            caGAAGAACGCCCAGATCACGGCAGGCAGGCCTTCGATGATGAACATCCAGCGCCAGTCGTAATGCTTGATCAGGTAACCGGACACCACCGACATCCACACAATGGTCACCGGGTTGCCCAGCATCAGGAAGgtgt                                                                                                      <  1:1147062/136‑1 (MQ=255)
                                       ccAGATCACGGCAGGCAGGCCTTCGATGATGAACATCCAGCGCCAGTCGTAATGCTTGATCAGGTAACCGGACACCACCGACATCCACACAATGGTCACCGGGTTGCCCAGCATCAGGAAGGTGTTGGCGCGTGaa                                                                                           >  1:791989/1‑136 (MQ=255)
                                                    ggcaggcCTTCGATGATGAACATCCAGCGCCAGTCGTAATGCTTGATCAGGTAACCGGACACCACCGACATCCACACAATGGTCACCGGGTTGCCCAGCATCAGGAAGGTGTTGGCGCGTGAACGCTCGGCCCggg                                                                              <  1:556163/136‑1 (MQ=255)
                                                        ggcCTTCGATGATGAACATCCAGCGCCAGTCGTAATGCTTGATCAGGTAACCGGACACCACCGACATCCACACAATGGTCACCGGGTTGCCCAGCATCAGGAAGGTGTTGGCGCGTGAACGCTCGGCCCGGGTGaa                                                                          >  1:484148/1‑136 (MQ=255)
                                                         gcCTTCGATGATGAACATCCAGCGCCAGTCGTAATGCTTGATCAGGTAACCGGACACCACCGACATCCACACAATGGTCACCGGGTTGCCCAGCATCAGGAAGGTGTTGGCGCGTGAACGCTCGGCCCGGGTGAAc                                                                         <  1:3071343/136‑1 (MQ=255)
                                                          ccTTCGATGATGAACATCCAGCGCCAGTCGTAATGCTTGATCAGGTAACCGGACACCACCGACATCCACACAATGGTCACCGGGTTGCCCAGCATCa                                                                                                               >  2:2811981/1‑97 (MQ=255)
                                                          ccTTCGATGATGAACATCCAGCGCCAGTCGTAATGCTTGATCAGGTAACCGGACACCACCGACATCCACACAATGGTCACCGGGTTGCCCAGCATCa                                                                                                               <  1:2811981/97‑1 (MQ=255)
                                                            ttCGATGATGAACATCCAGCGCCAGTCGTAATGCTTGATCAGGTAACCGGACACCACCGACATCCACACAATGGTCACCGGGTTGCCCAGCATCAGGAAGGTGTTGGCGCGTGAACGCTCGGCCCGGGTGAACCaa                                                                      <  1:2461409/136‑1 (MQ=255)
                                                              cGATGATGAACATCCAGCGCCAGTCGTAATGCTTGATCAGGTAACCGGACACCACCGACATCCACACAATGGTCACCGGGTTGCCCAGCATCAGGAAGGTGTTGGCGCGTGAACGCTCGGCCCGGGTGAACCAATg                                                                    <  1:1526189/136‑1 (MQ=255)
                                                                   atgaACATCCAGCGCCAGTCGTAATGCTTGATCAGGTAACCGGACACAACCGACATCCACACAAAGGTCACCGGGATGCCCAGCATCAGGAAGGTGTAGGCGCGTGAACGCTCGGCCCGGGAGAACCAAAGGcaca                                                               >  1:1209064/1‑136 (MQ=255)
                                                                      aaCATCCAGCGCCAGTCGTAATGCTTGATCAGGTAACCGGACACCACCGACATCCACACAATGGTCACCGGGTTGCCCAGCATCAGGAAGGTGTTGGCGCGTGAACGCTCGGCCCGGGTGAACCAATGGCACAGGt                                                            <  1:765220/136‑1 (MQ=255)
                                                                       aCATCCAGCGCCAGTCGTAATGCTTGATCAGGTAACCGGACACCACCGACATCCACACAATGGTCACCGGGTTGCCCAGCATCAGGAAGGTGTTGGCGCGTGAACGCTCGGCCCGGGTGAACCAATGGCACAGGTa                                                           >  1:482970/1‑136 (MQ=255)
                                                                       aCATCCAGCGCCAGTCGTAATGCTTGATCAGGTAACCGGACACCACCGACATCCACACAATGGTCACCGGGTTGCCCAGCATCAGGAAGGTGTTGGCGCGTGAACGCTCGGCCCGGGTGAACCAATGGCACAGGTa                                                           >  1:498208/1‑136 (MQ=255)
                                                                           ccAGCGCCAGTCGTAATGCTTGATCAGGTAACCGGACACCACCGACATCCACAGAATGGTCACCGGGTTGCCCAGCATCAGGAAGGTGTAGGCGCGTGAACGCTCGGCCCGGGTGAACCAATGGCACAGGTACAcc                                                       >  2:1473024/1‑136 (MQ=255)
                                                                            cAGCGCCAGTCGTAATGCTTGATCAGGTAACCGGACACCACCGACATCCACACAATGGTCACCGGGTTGCCCAGCATCAGGAAGGTGTTGGCGCGTGAACGCTCGGCCCGGGTGAACCAATGGCACAGGTACACCa                                                      <  1:1209333/136‑1 (MQ=255)
                                                                               cgcCAGTCGTAATGCTTGATCAGGTAACCGGACACCACCGACATCCACACAATGGTCACCGGGTTGCCCAGCATCAGGAAGGTGTTGGCGCGTGAACGCTCGGCCCGGGTGAACCAATGGCACAGGTACACCAGCa                                                   <  1:1845167/136‑1 (MQ=255)
                                                                                gcCAGTCGTAATGCTTGATCAGGTAACCGGACACCACCGACATCCACACAATGGTCACCGGGTTGCCCAGCATCAGGAAGGTGTTGGCGCGTGAACGCTCGGCCCGGGTGAACCAATGGCACAGGTACACCAGCAt                                                  >  2:1676663/1‑136 (MQ=255)
                                                                                gcCAGTCGTAATGCTTGATCAGGTAACCGGACACCACCGACATCCACACAATGGTCACCGGGTTGCCCAGCATCAGGAAGGTGTTGGCGCGTGAACGCTCGGCCCGGGTGAACCAATGGCACAGGTACACCAGCAt                                                  >  2:760111/1‑136 (MQ=255)
                                                                                 ccAGTCGTAATGCTTGATCAGGTAACCGGACACCACCGACATCCACACAATGGTCACCGGGTTGCCCAGCATCAGGAAGGTGTTGGCGCGTGAACGCTCGGCCCGGGTGAACCAATGGCACAGGTACACCAGCATg                                                 >  1:86277/1‑136 (MQ=255)
                                                                                 ccAGTCGTAATGCTTGATCAGGTAACCGGACACCACCGACATCCACACAATGGTCACCGGGTTGCCCAGCATCAGGAAGGTGTTGGCGCGTGAACGCTCGGCCCGGGTGAACCAATGGCACAGGTACACCAGCATg                                                 >  1:178180/1‑136 (MQ=255)
                                                                                  cAGTCGTAATGCTTGATCAGGTAACCGGACACCACCGACATCCACACAATGGTCACCGGGTTGCCCAGCATCAGGAAGGTGTTGGCGCGTGAACGCTCGGCCCGGGTGAACCAATGGCACAGGTACACCAGCATgg                                                >  1:615576/1‑136 (MQ=255)
                                                                                     gcGTAATGCTTGATTACTGATCCGGAGACCACCGAGATCTACACAATGGTCACCGGGTTGCCCAGCATCAGGAAGGTGTTGGCGCGTGAACGCTCGGCCCGGGTGAACCAATGGCACAGGTACACCAGCATGGCCg                                             <  2:1883483/135‑1 (MQ=255)
                                                                                      cGTAATGCTTGATCAGGTAACCGGACACCACCGACATCCACACAATGGTCACCGGGTTGCCCAGCATCAGGAAGCTGTTGGCGCGTGAACGCTCGGCCCGGGTGAACCAAAGGCACAGGTACACCAGCATGGCCgg                                            >  1:1883483/1‑136 (MQ=255)
                                                                                       gTAATGCTTGATCAGGTAACCGGACACCACCGACATCCACACAATGGTCACCGGGTTGCCCAGCATCAGGAAGGTGTTGGCGCGTGAACGCTCGGCCCGGGTGAACCAAt                                                                     >  2:1066418/1‑110 (MQ=255)
                                                                                       gTAATGCTTGATCAGGTAACCGGACACCACCGACATCCACACAATGGTCACCGGGTTGCCCAGCATCAGGAAGGTGTTGGCGCGTGAACGCTCGGCCCGGGTGAACCAAt                                                                     <  1:1066418/110‑1 (MQ=255)
                                                                                        tAATGCTTGATCAGGTAACCGGACACCACCGACATCCACACAATGGTCACCGGGTTGCCCAGCATCAGGAAGGTGTTGGCGCGTGAACGCTCGGCCCGGGTGAACCAATgg                                                                   <  1:2913551/111‑1 (MQ=255)
                                                                                        tAATGCTTGATCAGGTAACCGGACACCACCGACATCCACACAATGGTCACCGGGTTGCCCAGCATCAGGAAGGTGTTGGCGCGTGAACGCTCGGCCCGGGTGAACCAATgg                                                                   >  2:2913551/1‑111 (MQ=255)
                                                                                             cTTGATCAGGTAACCGGACACCACCGACATCCACACAATGGTCACCGGGTTGCCCAGCATCAGGAAGGTGTTGGCGCGTGAACGCTCGGCCCGGGTGAACCAATGGCACAGGTACACCAGCATGGCCGGCATGAcc                                     <  1:2326865/136‑1 (MQ=255)
                                                                                                  tCAGGTAACCGGACACCACCGACATCCACACAATGGTCACCGGGTTGCCCAGCATCAGGAAGGTGTTGGCGCGTGAACGCTCGGCCCGGGTGAACCAATGGCACAGGTACACCAGCATGGCCGGCATGACCGCAGc                                >  2:1889127/1‑136 (MQ=255)
                                                                                                  tCAGGTAACCGGACACCACCGACATCCACACAATGGTCACCGGGTTGCCCAGCATCAGGAAGGTGTTGGCGCGTGAACGCTCGGCCCGGGTGAACCAATGGCACAGGTACACCAGCATGGCCGGCATGACCGCAGc                                >  2:1936817/1‑136 (MQ=255)
                                                                                                   cAGGTAACCGGACACCACCGACATCCACACAATGGTCACCGGGTTGCCCAGCATCAGGAAGGTGTTGGCGCGTGAACGCTCGGCCCGGGTGAACCAATGGCACAGGTACACCAGCATGGCCGGCATGACCGCAGcc                               >  1:1086310/1‑136 (MQ=255)
                                                                                                         aCCGGACACCACCGACATCCACACAATGGTCACCGGGTTGCCCAGCATCAGGAAGGTGTTGGCGCGTGAACGCTCGGCCCGGGTGAACCAATGGCACAGGTACACCAGCATGGCCGGCATGACCGCAGCCTccacc                         >  2:1121342/1‑136 (MQ=255)
                                                                                                                 ccaccGACATCCACACAATGGTCACCGGGTTGCCCAGCATCAGGAAGGTGTTGGCGCGTGAACGCTCGGCCCGGGTGAACCAATGGCACAGGTACACCAGCATGGCCGGCATGACCGCAGCCTCCACCACCCCGAg                 <  1:477947/136‑1 (MQ=255)
                                                                                                                     cGACATCCACACAATGGTCACCGGGTTGCCCAGCATCAGGAAGGTGTTGGCGCGTGAACGCTCGGCCCGGGTGAACCAAt                                                                     >  1:700318/1‑80 (MQ=255)
                                                                                                                     cGACATCCACACAATGGTCACCGGGTTGCCCAGCATCAGGAAGGTGTTGGCGCGTGAACGCTCGGCCCGGGTGAACCAAt                                                                     <  2:700318/80‑1 (MQ=255)
                                                                                                                                gAATGGTCACCGGGTTGCCCAGCATCAGGAAGGTGTTGGCGCGTGAACGCTCGGCCCGGGTGAACCAATGGCACAGGTACACCAGCATGGCCGGCATGACCGCAGCCTCCACCACCCCGAGCAGGAAGCGGATGcc  <  2:1954394/136‑1 (MQ=255)
                                                                                                                                |                                                                                                                                       
GGCCGGGCGGTCATCCACCAGGCGCCACCAGAAGAACGCCCAGATCACGGCAGGCAGGCCTTCGATGATGAACATCCAGCGCCAGTCGTAATGCTTGATCAGGTAACCGGACACCACCGACATCCACAGAATGGTCACCGGGTTGCCCAGCATCAGGAAGGTGTTGGCGCGTGAACGCTCGGCCCGGGTGAACCAATGGCACAGGTACACCAGCATGGCCGGCATGACCGCAGCCTCCACCACCCCGAGCAGGAAGCGGATGCC  >  NC_002947/3824567‑3824830

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑