Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	4,717,498	C→G	A254G (GCC→GGC)	PP_4173 →	two‑component system sensor histidine kinase/response regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,717,498	0	C	G	100.0%	57.4 / NA	18	A254G (GCC→GGC)	PP_4173	two‑component system sensor histidine kinase/response regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (8/10);  total (8/10)

CAGCAAGGCGAGTACAACACCCCCTTGCCGGTCGTCGACGACAGCGAGCTGGGCCACTTGGCCCGCCACATCAATAACCTGGCCAGCGCCCTGGAGCAGGCCAGCGATGAACAGAAGCAGGCCATCGCCGAACTGATCCAGGCCCGCGAGGAGGCCGAACAGGCCAACCGTGCCAAATCGGATTTCCTGGCGATGATGAGCCATGAGTTGCGCACCCCGATGAATGGCGTGCTGGGCATGCTGCAACTGCTGGAAACCACACCGCTGA  >  NC_002947/4717356‑4717623                                                                                                                                               |                                                                                                                              cAGCAAGGCGAGTACAACACCCCCTTGCCGGTCGTCGACGACAGCGAGCTGGGCCACTTGGCCCGCCACATCAATAACCTGGCCAGCGCCCTGGAGCAGGCCAGCGATGAACAGAAGCAGGCCATCGCCGAACTGATCCAGGGCCGCgag                                                                                                                        <  2:85401/150‑1 (MQ=255) cAGCAAGGCGAGTACAACACCCCCTTGCCGGTCGTCGACGACAGCGAGCTGGGCCACTTGGCCCGCCACATCAATAACCTGGCCAGCGCCCTGGAGCAGGCCAGCGATGAACAGAAGCAGGCCATCGCCGAACTGATCCAGGGCCGCgag                                                                                                                        <  2:85386/150‑1 (MQ=255)               caacaCCCCCTTGCCGGTCGTCGACGACAGCGAGCTGGGCCACTTGGCCCGCCACATCAATAACCTGGCCAGCGCCCTGGAGCAGGCCAGCGATGAACAGAAGCAGGCCATCGCCGAACTGATCCAGGGCCGCGAGGAGGCCGAACAGGc                                                                                                          <  2:102708/150‑1 (MQ=255)                  cacCCCCTTGCCGGTCGTCGACGACAGCGAGCTGGGCCACTTGGCCCGCCACATCAATAACCTGGCCAGCGCCCTGGAGCAGGCCAGCGATGAACAGAAGCAGGCCATCGCCGAACTGATCCAGGGCCGCGAGGAGGCCGAACAGGCCaa                                                                                                       <  2:28745/150‑1 (MQ=255)                     ccccTTGCCGGTCGTCGACGACAGCGAGCTGGGCCACTTGGCCCGCCACATCAATAACCTGGCCAGCGCCCTGGAGCAGGCCAGCGATGAACAGAAGCAGGCCATCGCCGAACTGATCCAGGGCCGCGAGGAGGCCGAACAGGCCAACCg                                                                                                    >  1:361413/1‑150 (MQ=255)                       ccTTGCCGGTCGTCGACGACAGCGAGCTGGGCCACTTGGCCCGCCACATCAATAACCTGGCCAGCGCCCTGGAGCAGGCCAGCGATGAACAGAAGCAGGCCATCGCCGAACTGATCCAGGGCCGCGAGGAGGCCGAACAGGCCAACCGTg                                                                                                  >  2:149670/1‑150 (MQ=255)                        cTTGCCGGTCGTCGACGACAGCGAGCTGGGCCACTTGGCCCGCCACATCAATAACCTGGCCAGCGCCCTGGAGCAGGCCAGCGATGAACAGAAGCAGGCCATCGCCGAACTGATCCAGGGCCGCGAGGAGGCCGAACAGGCCAACCGTGc                                                                                                 <  1:233083/150‑1 (MQ=255)                        cTTGCCGGTCGTCGACGACAGCGAGCTGGGCCACTTGGCCCGCCACATCAATAACCTGGCCAGCGCCCTGGAGCAGGCCAGCGATGAACAGAAGCAGGCCATCGCCGAACTGATCCAGGGCCGCGAGGAGGCCGAACAGGCCAACCGTGc                                                                                                 >  1:176569/1‑150 (MQ=255)                                                   gggCCACTTGGCCCGCCACATCAATAACCTGGCCAGCGCCCTGGAGCAGGCCAGCGATGAACAGAAGCAGGCCATCGCCGAACTGATCCAGGGCCGCGAGGAGGCCGAACAGGCCAACCGTGCCAAATCGGATTTCCTGGCGATGATGAg                                                                      >  1:370275/1‑150 (MQ=255)                                                   gggCCACTTGGCCCGCCACATCAATAACCTGGCCAGCGCCCTGGAGCAGGCCAGCGATGAACAGAAGCAGGCCATCGCCGAACTGATCCAGGGCCGCGAGGAGGCCGAACAGGCCAACCGTGCCAAATCGGATTTCCTGGCGATGATGAg                                                                      >  1:370276/1‑150 (MQ=255)                                                                  ccACATCAATAACCTGGCCAGCGCCCTGGAGCAGGCCAGCGATGAACAGAAGCAGGCCATCGCCGAACTGATCCAGGGCCGCGAGGAGGCCGAACAGGCCAACCGTGCCAAATCGGATTTCCTGGCGATGATGAGCCATGAGTTGCGCAc                                                       >  1:126276/1‑150 (MQ=255)                                                                                     aGCGCCCTGGAGCAGGCCAGCGATGAACAGAAGCAGGCCATCGCCGAACTGATCCAGGGCCGCGAGGAGGCCGAACAGGCCAACCGTGCCAAATCGGATTTCCTGGCGATGATGAGCCATGAGTTGCGCACCCCGATGAATGGCGTGCTg                                    <  1:149670/150‑1 (MQ=255)                                                                                          ccTGGAGCAGGCCAGCGATGAACAGAAGCAGGCCATCGCCGAACTGATCCAGGGCCGCGAGGAGGCCGAACAGGCCAACCGTGCCAAATCGGATTTCCTGGCGATGATGAGCCATGAGTTGCGCACCCCGATGAATGGCGTGCTGGGCAt                               >  1:207450/1‑150 (MQ=255)                                                                                            tGGAGCAGGCCAGCGATGAACAGAAGCAGGCCATCGCCGAACTGATCCAGGGCCGCGAGGAGGCCGAACAGGCCAACCGTGCCAAATCGGATTTCCTGGCGATGATGAGCCATGAGTTGCGCACCCCGATGAATGGCGTGCTGGGCAtgc                             <  2:370275/150‑1 (MQ=255)                                                                                            tGGAGCAGGCCAGCGATGAACAGAAGCAGGCCATCGCCGAACTGATCCAGGGCCGCGAGGAGGCCGAACAGGCCAACCGTGCCAAATCGGATTTCCTGGCGATGATGAGCCATGAGTTGCGCACCCCGATGAATGGCGTGCTGGGCAtgc                             <  2:370276/150‑1 (MQ=255)                                                                                                                       aGGCCATCGCCGAACTGATCCAGGGCCGCGAGGAGGCCGAACAGGCCAACCGTGCCAAATCGGATTTCCTGGCGATGATGAGCCATGAGTTGCGCACCCCGATGAATGGCGTGCTGGGCATGCTGCAACTGCTGGAAACCACACCGCTGa  <  2:46975/150‑1 (MQ=255)                                                                                                                                        aTCCAGGGCCGCGAGGAGGCCGAACAGGCCAACCGTGCCAAATCGGATTTCCTGGCGATGATGAGCCATGAGTTGCGCACCCCGATGAATGGCGTGCt                                     <  1:193122/98‑1 (MQ=255)                                                                                                                                        aTCCAGGGCCGCGAGGAGGCCGAACAGGCCAACCGTGCCAAATCGGATTTCCTGGCGATGATGAGCCATGAGTTGCGCACCCCGATGAATGGCGTGCt                                     >  2:193122/1‑98 (MQ=255)                                                                                                                                               |                                                                                                                              CAGCAAGGCGAGTACAACACCCCCTTGCCGGTCGTCGACGACAGCGAGCTGGGCCACTTGGCCCGCCACATCAATAACCTGGCCAGCGCCCTGGAGCAGGCCAGCGATGAACAGAAGCAGGCCATCGCCGAACTGATCCAGGCCCGCGAGGAGGCCGAACAGGCCAACCGTGCCAAATCGGATTTCCTGGCGATGATGAGCCATGAGTTGCGCACCCCGATGAATGGCGTGCTGGGCATGCTGCAACTGCTGGAAACCACACCGCTGA  >  NC_002947/4717356‑4717623

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑