| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 2,246,373 | C→G | intergenic (+5/‑47) | PP_1980 → / → dusC | hypothetical protein/tRNA‑dihydrouridine synthase DusC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,246,373 | 0 | C | G | 95.0% | 67.4 / NA | 20 | intergenic (+5/‑47) | PP_1980/dusC | hypothetical protein/tRNA‑dihydrouridine synthase DusC |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base G (10/9); minor base T (0/1); total (10/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCGCGATGGTGTGACGCTGCTGCGTGCGCAAACCACCTTCGCCTGCATCGAGCTGTCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGCCCTGGCCCCCATGGAGGGGCTGGTCGACAACATCCTGCGCGACGTATTGACTCGTGTGGGCGGTATCGACTGGT > NC_002947/2246242‑2246504 | gcgcgATGGTGTGACGCTGCTGCGTGCGCAAACCACCTTCGCCTGCATCGAGCTGTCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGggcggc > 1:196028/1‑136 (MQ=255) cgtgcgCAAACCACCTTCGCCTGCATCGAGCTGTCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGGCGGCATTTTTTGTTAAACTGCCGGCt > 2:431831/1‑136 (MQ=255) cgtgcgCAAACCACCTTCGCCTGCATCGAGCTGTCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGGCGGCATTTTTTGTTAAACTGCCGGCt > 1:522010/1‑136 (MQ=255) cgtgcgCAAACCACCTTCGCCTGCATCGAGCTGTCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGGCGGCATTTTTTGTTAAACTGCCGGCt > 1:472352/1‑136 (MQ=255) accTTCGCCTGCATCGAGCTGTCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGGCGGCAATTTTTGTTAAACTGCCGGCTTTTTCCGCTgag > 2:229499/1‑136 (MQ=255) cGCCTGCATAGAGCTGTCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGGCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGAcccc < 2:69251/136‑1 (MQ=255) cGAGCTGTCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGGCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCaa > 1:384524/1‑136 (MQ=255) tGTCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGGCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGc < 1:16377/136‑1 (MQ=255) ccAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGGCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGCCCt < 1:431831/136‑1 (MQ=255) gCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGGCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGCCCTGGc < 2:196028/136‑1 (MQ=255) ccAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGGCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGCCCTGGCCCCCATGGa > 2:19479/1‑136 (MQ=255) ccAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGGCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGCCCTGGCCCCCATGGa > 2:230606/1‑136 (MQ=255) ccAGGCGCATGCCGGCGGAATTCATCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGGCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGCCCTGGCCCCCATGGa > 2:130950/1‑136 (MQ=255) gcATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGGCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGCCCTGGCCCCCATGGAGGGGCt < 1:326680/136‑1 (MQ=255) gcATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGGCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGCCCTGGCCCCCATGGAGGGGCt < 2:472352/136‑1 (MQ=255) aaTTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGGCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGCCCTGGCCCCCATGGAGGGGCTGGTCGACAACAt < 1:475990/136‑1 (MQ=255) tACGGTCCGGCGCTGATCGGCGCCTGAGTGGGCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGCCCTGGCCCCCATGGAGGGGCTGGTCGACAACATCCTGCGCGACGTAt < 1:391896/136‑1 (MQ=255) cgcTGATCGGCGCCTGAGTGGGCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGCCCTGGCCCCCATGGAGGGGCTGGTCGACAACATCCTGCGCGACGTATTGACTCgtgt > 1:440740/1‑136 (MQ=255) ggCGCCTGAGTGGGCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGCCCTGGCCCCCAAGGAGGGGCTGGTCGACAACATCCTGCGCGACGTATTGAATCGTGTGGGCGGtt > 1:471593/1‑135 (MQ=255) cgcCTGAGTGGGCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGCCCTGGCCCCCATGGAGGGGCTGGTCGACAACATCCTGCGCGACGTATTGACTCGTGTGGGCGGTATc < 2:335803/136‑1 (MQ=255) gTGGTCGGCATTTTTTGTTAAACTGCCTGCTTTTTCCGCTGAGACCCCGACATGCAAATTGCCCTGGCCCCCATGGAGGGGCTGGTCGACAACATCCTGCGCGACGTATTGACTCGTGTGGGCGGTATCGACTGGt < 1:229499/136‑1 (MQ=255) | GCGCGATGGTGTGACGCTGCTGCGTGCGCAAACCACCTTCGCCTGCATCGAGCTGTCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGCCCTGGCCCCCATGGAGGGGCTGGTCGACAACATCCTGCGCGACGTATTGACTCGTGTGGGCGGTATCGACTGGT > NC_002947/2246242‑2246504 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |