| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 4,160,437 | A→C | S161R (AGC→CGC) | PP_3661 → | membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,160,437 | 0 | A | C | 84.6% | 12.4 / ‑5.1 | 13 | S161R (AGC→CGC) | PP_3661 | membrane protein |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base C (5/6); total (6/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGATCGGCCTGTGGTGTGGCATGAAAGTGTTCAAGCTCGACCGCGATACCGCCTTGCTGACCGCCGCCGGCAGCGCCATCTGCGGCGCCGCCGCCGTGCTGGCATTCGAATCGGCGCTGCGCAGCGCCCCCCATAAAAGCGCCATGGCAGTCGGAAGCGTAGTGCTGTTCGGTACCTTGTCGATGTTCCTTTACCCACTGGCGATCAATGCCGGCTGGCTGCATCTGGATACCATGGGCGCCGGGC > NC_002947/4160315‑4160560 | tgATCGGCCTGTGGTGTGGCATGAAAGTGTTCAAGCTCGACCGCGATACCGCCTTGCTGACCGCCGCCGGCAGCGCCATCTGCGGCGCCGCCGCCGTGCTGGCATTCGAATCGGGGGGGGGGCGCGccccccccaa > 2:513090/1‑132 (MQ=255)tgATCGGCCTGTGGTGTGGCATGAAAGTGTTCAAGCTCGACCGCGATACCGCCTTGCTGACCGCCGCCGGCAGCGCCATCTGCGGCGCCGCCGCCGTGCTGGCATTCGAATCGGGGGGGCGCCCCGcccccccaaa > 1:193945/1‑132 (MQ=255)tgATCGGCCTGTGGTGTGGCATGAAAGTGTTCAAGCTCGACCGCGATACCGCCTTGCTGACCGCCGCCGGCAGCGCCATCTGCGGCGCCGCCGCCGTGCTGGCATTCGAATCGGCGCGGCGCCCCGcccccccgaa > 2:222678/1‑132 (MQ=255) ggCCTGTGGTGTGGCATGAAAGTGTTCAAGCTCGACCGCGATACCGCCTTGCTGACCGCCGCCGGCAGCGCCATCTGCGGCGCCGCCGCCGTGCTGGCATTCGAATCGGCGCTGCGCAGAGCCCCCCCGaaaaacc > 2:146795/1‑133 (MQ=255) cTGTGGTGTGGCATGAAAGTGTTCAAGCTCGACCGCGATACCGCCTTGCTGACCGCCGCCGGCAGCGCCAACTGCGGCGCCGCCGCCGTGCTGGCATTCGAATCGGCGCTGGGCCCCGCCCCCCCAaaaatccccc > 2:528924/1‑130 (MQ=255) gtggCTTTAATGTTTCCACGCTCCACCGCTATCCCGCCTTGCTGCCCGCCGCCGGCCGCGCCATCGGCGGCGCCGCCGCCGTGCGGGCATTCGACGCGGCGCTGCGCCGCGCCCCCCATAAAAGCGCCATGGCAGt < 2:289664/136‑1 (MQ=255) cATGAAGGTGTTCAAGCTCGACCGCGATACCGCCTTGCTGACCGCCGCCGGCAGAGCCATCTGCGGCGCCGCCGCCGTGCTGGCATTCGAATCGGCGCGGCGCCGCGCCCCCCCACAAATCGCCAAGGCAGTCggg > 1:273794/1‑135 (MQ=255) tgtgtTCAAGCCCCCCCGCGATCCCGCCTGGCGCCCCGCCGCCGGCAGCGCCAGCGGCGGCGCCGCCGCGGTGCTGGCATTCTACGCGGCGCGGCGCCGCCCCCCCCATAAAAGCGCCATGGCAGTCGGAAGCGTa < 2:196792/135‑1 (MQ=255) ggcggcgCCGCCGCCGTGCTGGCCTGCGCCGCGGCGCGGCGCCGCCCCCCCCATAAAAGCGCCATGGCAGTCGGAAGCGTAGTGCTGTTCGGTACCTTGTCGATGTTCCTTTACCCACTGGCGATCAATGCCggct < 1:246623/135‑1 (MQ=255) cggcgCCGCCGCGGGGCTGCCTTTCTAATCGGCGCTGCGCAGCGCCCCCCATAAAAGCGCCATGGCAGTCGGAAGCGTAGTGCTGTTCGGTACCTTGTCGATGTTCCTTTACCCACTGGCGATCAATGCCggctgg < 2:554539/136‑1 (MQ=255) gcgcCGCCGCGTTGCTTGATTTGTATTCGGGGCGGCGTCGCGCCCCCCATAAAAGCGCCATGGCAGTCGGAAGCGTAGTGCTGTTCGGTACCTTGTCGATGTTCCTTTACCCACTGGCGATCAATGCCggctggct < 2:317746/136‑1 (MQ=255) ccggggTGCTGGCTTTCGCTGCGGCGCGGCGCCGCGCCCCCCATAAAAGCGCCATGGCAGTCGGAAGCGTAGTGCTGTTCGGTACCTTGTCGATGTTCCTTTACCCACTGGCGATCAATGCCGGCTGGCTGCATCt < 1:366891/131‑1 (MQ=255) gcGGCGCCGCGCCCCCCCCCCCATAAAAGCGCCATGGCAGTCGGAAGCGTAGTGCTGTTCGGTACCTTGTCGATGTTCCTTTACCCACTGGCGATCAATGCCGGCTGGCTGCATCTGGATACCATGGGCGCCGGgc < 1:385480/135‑1 (MQ=255) | TGATCGGCCTGTGGTGTGGCATGAAAGTGTTCAAGCTCGACCGCGATACCGCCTTGCTGACCGCCGCCGGCAGCGCCATCTGCGGCGCCGCCGCCGTGCTGGCATTCGAATCGGCGCTGCGCAGCGCCCCCCATAAAAGCGCCATGGCAGTCGGAAGCGTAGTGCTGTTCGGTACCTTGTCGATGTTCCTTTACCCACTGGCGATCAATGCCGGCTGGCTGCATCTGGATACCATGGGCGCCGGGC > NC_002947/4160315‑4160560 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |