Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 3,827,923 G→T R30S (CGT→AGT)  ptxS ← 2‑ketogluconate utilization repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,827,9230GT98.3% 1069.2 / 4.2 295R30S (CGT→AGT) ptxS2‑ketogluconate utilization repressor
Reads supporting (aligned to +/- strand):  ref base G (4/1);  new base T (121/169);  total (125/170)
Fisher's exact test for biased strand distribution p-value = 1.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01

Only 100 of 299 total aligned reads displayed.

CGGCCACCAGCATGCCGATCAGTCGCGTCTGGCCGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGTGATGAAACCGGTTTCAATACAGCAAAAATGC  >  NC_002947/3827776‑3828069
                                                                                                                                                   |                                                                                                                                                  
cGGCCACCAGCATGCCGATCAGTCGCGTCTGGCCGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACtgg                                                                                                                                                  <  1:3934883/150‑1 (MQ=255)
 ggCCACCAGCATGCCGATCAGTCGCGTCTGGCCGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACTGga                                                                                                                                                 >  1:3151633/1‑150 (MQ=255)
 ggCCACCAGCATGCCGATCAGTCGCGTCTGGCCGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACTGga                                                                                                                                                 >  2:2810671/1‑150 (MQ=255)
 ggCCACCAGCATGCCGATCAGTCGCGTCTGGCCGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACTGga                                                                                                                                                 <  2:4447825/150‑1 (MQ=255)
 ggCCACCAGCATGCCGAACAGTCGCGTCTGGCCGCGCTTTAGGCCGCGAGCCATCTGCTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACTGga                                                                                                                                                 <  1:4194970/150‑1 (MQ=255)
   ccaccaGCATGCCGATCAGTCGCGTCTGGCCGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACTGgaga                                                                                                                                               <  1:3231316/150‑1 (MQ=255)
    caccaGCATGCCGATCAGTCGCGTCTGGCCGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACTGGAGAc                                                                                                                                              >  2:1218725/1‑150 (MQ=255)
      ccaGCATGCCGATCAGTCGCGTCTGGCCGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACTGGAGACgg                                                                                                                                            <  1:4752408/150‑1 (MQ=255)
      ccaGCATGCCGATCAGTCGCGTCTGGCCGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACTGGAGACgg                                                                                                                                            <  2:514108/150‑1 (MQ=255)
        aGCATGCCGATCAGTCGCGTCTGGCCGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACTGGAGACGGTg                                                                                                                                          <  2:768196/150‑1 (MQ=255)
        aGCATGCCGATCAGTCGCGTCTGGCCGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACTGGAGACGGTg                                                                                                                                          >  1:3875376/1‑150 (MQ=255)
           aTGCCGATCAGTCGCGTCTGGCCGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGACGGTGGCt                                                                                                                                       >  1:806679/1‑150 (MQ=255)
           aTGCCGATCAGTCGCGTCTGGCCGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGACGGTGGCt                                                                                                                                       >  1:2505185/1‑150 (MQ=255)
             gCCGATCAGTCGCGTCTGGCCGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACTGGAGACGGTTGCttt                                                                                                                                     >  1:4641510/1‑150 (MQ=255)
             gCCGATCAGTCGCGTCTGGCCGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACTGGAGACGGTGGCttt                                                                                                                                     >  1:3018576/1‑150 (MQ=255)
             gCCGATCAGTCGCGTCTGGCCGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACTGGAGACGGTGGCttt                                                                                                                                     <  2:3791161/150‑1 (MQ=255)
              ccGATCAGTCGCGTCTGGCCGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCGGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACTGGAGACGGTGGCTTTg                                                                                                                                    <  1:1596614/150‑1 (MQ=255)
               cGATCAGTCGCGTCTGGCCGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACTGGAGACGGTGGCTTTgg                                                                                                                                   >  2:619862/1‑150 (MQ=255)
               cGATCAGTCGCGTCTGGCCGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACTGGAGACGGTGGCTTTgg                                                                                                                                   >  2:1421706/1‑150 (MQ=255)
                gATCAGTCGCGTCTGGCCGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACTGGAGACGGTGGCTTTGGa                                                                                                                                  <  1:3681909/150‑1 (MQ=255)
                  tCAGTCGCGTCTGGCCGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACTGGAGACGGTGGCTTTGGaaa                                                                                                                                <  2:470936/150‑1 (MQ=255)
                   cAGTCGCGTCTGGCCGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACTGGAGACGGTGGCTTTGGAAAc                                                                                                                               <  2:4384521/150‑1 (MQ=255)
                     gTCGCGTCTGGCCGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACTGGAGACGGTGGCTTTGGAAACGc                                                                                                                             >  2:2854491/1‑150 (MQ=255)
                     gTCGCGTCTGGCCGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACTGGAGACGGTGGCTTTGGAAACGc                                                                                                                             <  1:4441390/150‑1 (MQ=255)
                       cgcgTCTGGCCGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACTGGAGACGGTGGCTTTGGAAACGCCa                                                                                                                           <  2:1798742/150‑1 (MQ=255)
                       cgcgTCTGGCCGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACTGGAGACGGTGGCTTTGGAAACGCCa                                                                                                                           <  2:4296765/150‑1 (MQ=255)
                       cgcgTCTGGCCGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACTGGAGACGGTGGCTTTGGAAACGCCa                                                                                                                           <  2:3529057/150‑1 (MQ=255)
                          gTCTGGCCGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACTGGAGACGGTGGCTTTGGAAACGCCa                                                                                                                           >  2:3592222/1‑147 (MQ=255)
                          gTCTGGCCGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACTGGAGACGGTGGCTTTGGAAACGCCAGCa                                                                                                                        >  2:1882947/1‑150 (MQ=255)
                            cTGGCCGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACTGGAGACGGTGGCTTTGGAAACGCCAGCAac                                                                                                                      <  2:16830/150‑1 (MQ=255)
                            cTGGCCGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACTGGAGACGGTGGCTTTGGAAACGCCAGCAac                                                                                                                      <  2:17176/150‑1 (MQ=255)
                              ggCCGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACTGGAGACGGTGGCTTTGGAAACGCCAGCAacac                                                                                                                    >  1:4581814/1‑150 (MQ=255)
                               gCCGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACTGGAGACGGTGGCTTTGGAAACGCCAGCAACAcg                                                                                                                   >  1:3291447/1‑150 (MQ=255)
                                ccGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACTGGAGACGGTGGCTTTGGAAACGCCAGCAACAcgc                                                                                                                  <  1:2493073/150‑1 (MQ=255)
                                ccGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACTGGAGACGGTGGCTTTGGAAACGCCAGCAACAcgc                                                                                                                  >  1:5006663/1‑150 (MQ=255)
                                            ccGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACTGGAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTg                                                                                                      >  1:3578408/1‑150 (MQ=255)
                                             cgcgAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACTGGAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGa                                                                                                     >  2:2946199/1‑150 (MQ=255)
                                              gcgAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACTGGAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGAt                                                                                                    <  1:5155646/150‑1 (MQ=255)
                                              gcgAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACTGGAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGAt                                                                                                    <  1:2420738/150‑1 (MQ=255)
                                              gcgAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACTGGAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGAt                                                                                                    <  1:2639274/150‑1 (MQ=255)
                                               cgAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACTGGAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATg                                                                                                   <  2:387145/150‑1 (MQ=255)
                                                 aGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACTGGAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGt                                                                                                 <  2:73167/150‑1 (MQ=255)
                                                 aGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACTGGAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGt                                                                                                 <  2:2067010/150‑1 (MQ=255)
                                                  gCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACTGGAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTg                                                                                                <  1:920078/150‑1 (MQ=255)
                                                   ccATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACTGGAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGa                                                                                               >  2:3486229/1‑150 (MQ=255)
                                                    cATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACTGGAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGAc                                                                                              >  1:5212867/1‑150 (MQ=255)
                                                    cATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACTGGAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGAc                                                                                              <  1:182565/150‑1 (MQ=255)
                                                    cATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACTGGAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGAc                                                                                              <  1:3390799/150‑1 (MQ=255)
                                                     aTCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACTGGAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGAcc                                                                                             >  1:5370002/1‑150 (MQ=255)
                                                     aTCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACTGGAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGAcc                                                                                             >  1:2826117/1‑150 (MQ=255)
                                                           ttGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACTGGAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTcg                                                                                       <  2:4240053/150‑1 (MQ=255)
                                                                   aTAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACTGGAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATg                                                                               <  1:543070/150‑1 (MQ=255)
                                                                    tAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACTGGAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATgg                                                                              <  1:313449/150‑1 (MQ=255)
                                                                    tAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACTGGAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATgg                                                                              <  1:1062815/150‑1 (MQ=255)
                                                                     cgCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACTGGAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATggg                                                                             <  1:244572/149‑1 (MQ=255)
                                                                      gCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACTGGAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGc                                                                            <  2:1491581/150‑1 (MQ=255)
                                                                              gTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACTGGAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGt                                                                    <  2:5229673/150‑1 (MQ=255)
                                                                                       ccTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACTGGAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATgg                                                           <  2:2335383/150‑1 (MQ=255)
                                                                                        ctctTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACTGGAGACGGTGGCTTTGGAAACGCCAGTAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATggg                                                          >  2:5378742/1‑150 (MQ=255)
                                                                                        ctctTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACTGGAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATggg                                                          >  1:809774/1‑150 (MQ=255)
                                                                                        ctctTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACTGGAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATggg                                                          >  2:5066551/1‑150 (MQ=255)
                                                                                         tctTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACTGGAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGt                                                         >  1:2146740/1‑150 (MQ=255)
                                                                                             ccAGCCGCTTGGCCGTGGCTTCGGCCAGCAAGTGGCGGTCGCCACCGATGTAACTGGAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTc                                                     >  2:3103848/1‑150 (MQ=255)
                                                                                              cAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTACCTGGAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCCCTTCACTGATGGTGACCCGTTCGCGGGTCTGGGCAGGCGCGTCGGTCATGGGTTTTcc                                                    >  2:4646002/1‑150 (MQ=255)
                                                                                               aGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACTGGAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCt                                                   <  1:4769451/150‑1 (MQ=255)
                                                                                                gCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACTGGAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCtt                                                  <  2:2507096/150‑1 (MQ=255)
                                                                                                 ccGCTTGGCCGTGGCTTCGTCCAGCATTTGGCGGTCGCCACCTATTTAACTGGAGACGGTGTCTTTGGAAACGCCAGCTACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTa                                                 <  1:55525/150‑1 (MQ=255)
                                                                                                  cGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACTGGAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTAt                                                <  1:1520862/150‑1 (MQ=255)
                                                                                                    cTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACTGGAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGa                                              <  2:3061527/150‑1 (MQ=255)
                                                                                                    cTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACTGGAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGa                                              <  2:3943333/150‑1 (MQ=255)
                                                                                                     caattgctGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACTGGAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGAt                                             >  1:2742927/8‑150 (MQ=255)
                                                                                                      tGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACTGGAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGAtt                                            <  2:3151626/150‑1 (MQ=255)
                                                                                                        gCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACTGGAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGt                                          <  2:1351848/150‑1 (MQ=255)
                                                                                                           gTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACTGGAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGtttt                                       >  2:3047792/1‑150 (MQ=255)
                                                                                                             ggCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACTGGAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTaa                                     <  1:3663844/150‑1 (MQ=255)
                                                                                                              gCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACTGGAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAAt                                    >  2:919839/1‑150 (MQ=255)
                                                                                                               cTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACTGGAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATa                                   <  1:1132878/150‑1 (MQ=255)
                                                                                                               cTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACTGGAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATa                                   <  1:1707778/150‑1 (MQ=255)
                                                                                                                 tCGGCCAGCAATTGGCGGTCGCCACCGATGTAACTGGAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGt                                 <  2:460483/150‑1 (MQ=255)
                                                                                                                   ggCCAGCAATTGGCGGTCGCCACCGATGTAACTGGAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGtga                               <  2:2146740/150‑1 (MQ=255)
                                                                                                                    gCCAGCAATTGGCGGTCGCCACCGATGTAACTGGAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGTGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGtgat                              >  1:126147/1‑150 (MQ=255)
                                                                                                                    gCCAGCAATTGGCGGTCGCCACCGATGTAACTGGAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGtgat                              <  2:2076714/150‑1 (MQ=255)
                                                                                                                    gCCAGCAATTGGCGGTCGCCACCGATGTAACTGGAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGATCATGGGTTTTCCTTATGATTGTTTTAATAGtgat                              <  1:280695/150‑1 (MQ=255)
                                                                                                                      cAGCAATTGGCGGTCGCCACCGATGTAACTGGAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGtgatga                            >  2:1137212/1‑150 (MQ=255)
                                                                                                                           aTTGGCGGTCGCCACCGATGTAACTGGAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGTGATGAAACCg                       <  2:5017145/150‑1 (MQ=255)
                                                                                                                               gCGGTCGCCACCGATGTAACTGGAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGTGATGAAACCGGttt                   >  2:2697423/1‑150 (MQ=255)
                                                                                                                               gCGGTCGCCACCGATGTAACTGGAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGTGATGAAACCGGttt                   <  2:1299586/150‑1 (MQ=255)
                                                                                                                               gCGGTCGCCACCGATGTAACTGGAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGTGATGAAACCGGttt                   <  1:1961140/150‑1 (MQ=255)
                                                                                                                                 ggTCGCCACCGATGTAACTGGAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGTGATGAAACCGGTTTCa                 <  2:3672591/150‑1 (MQ=255)
                                                                                                                                  gTCGCCACCGATGTAACTGGAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGTGATGAAACCGGTTTCaa                <  2:4880117/150‑1 (MQ=255)
                                                                                                                                  gTCGCCACCGATGTAACTGGAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGTGATGAAACCGGTTTCaa                <  1:2280240/150‑1 (MQ=255)
                                                                                                                                   tCGCCACCGATGTAACTGGAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGTGATGAAACCGGTTTCAAt               <  2:3969479/150‑1 (MQ=255)
                                                                                                                                   tCGCCACCGATGTAACTGGAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGTGATGAAACCGGTTTCAAt               <  1:4936740/150‑1 (MQ=255)
                                                                                                                                    cGCCACCGATGTAACTGGAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGTGATGAAACCGGTTTCtata              <  2:4758191/150‑1 (MQ=255)
                                                                                                                                    cGCCACCGATGTAACTGGAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGTGATGAAACCGGTTTCAATa              <  1:4114603/150‑1 (MQ=255)
                                                                                                                                    cGCCACCGATGTAACTGGAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGTGATGAAACCGGTTTCAATa              <  2:2400175/150‑1 (MQ=255)
                                                                                                                                     gCCACCGATGTAACTGGAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGTGATGAAACCGGTTTCAATAc             >  1:3979447/1‑150 (MQ=255)
                                                                                                                                      ccACCGATGTAACTGGAGACGGTGGCTTTGGAAACGCCAGCAACACGCTCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGTGATGAAACCGGTTTCAATACa            <  2:5054641/150‑1 (MQ=255)
                                                                                                                                        aCCGATGTAACTGGAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGTGATGAAACCGGTTTCAATACAGc          >  1:2855138/1‑150 (MQ=255)
                                                                                                                                                aaCTGGAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAg                                                                          >  2:3245441/1‑78 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CGGCCACCAGCATGCCGATCAGTCGCGTCTGGCCGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGTGATGAAACCGGTTTCAATACAGCAAAAATGC  >  NC_002947/3827776‑3828069

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑