Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	3,823,901	+T	coding (1248/1293 nt)	kguT ←	2‑ketogluconate transporter, putative

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,823,901	1	.	T	100.0%	126.1 / NA	36	S416S (TCT→TCA)	kguT	2‑ketogluconate transporter, putative
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (15/21);  total (15/21)

GGTCACCTCGACGCGATCTTGCAGGCGTGCCATCAAGTCGTCGGACAGGCGTTTATACAGGACGATTCGCTTCTTCATTGCAGGTTTCCTAATGGTTCATGGCCAGACGCGAGCTATCGCGCTTTGCCCGGCCA‑GAGGTCTGGGAAGTATTGAGCGCAGCGGTAAGCGCCACTGCAGTCAGCAGCGCACCACACATGAACAGGTAAGAGGCGCCGGGCCCGCCCGTAGCCCCATTGAGGTAGCCCACCAGCCACGAGCCGCC  >  NC_002947/3823768‑3824029                                                                                                                                       |                                                                                                                                 ggTCACCTCGACGCGATCTTGCAGGCGTGCCATCAAGTCGTCGGACAGGCGTTTATACAGGACGATTCGCTTCTTCATTGCAGGTTTCCTAATGGTTCATGGCCAGACGCGAGCTATCGCGCTTTGCCCGGCCatg                                                                                                                                 >  1:483590/1‑134 (MQ=255)  gTCACCTCGACGCGATCTTGCAGGCGTGCCATCAAGTCGTCGGACAGGCGTTTATACAGGACGATTCGCTTCTTCATTGCAGGTTTCCTAATGGTTCATGGCCAGACGCGAGCTATCGCGCTTTGCCCGGCCatga                                                                                                                                <  1:31081/136‑4 (MQ=255)    cACCTCGACGCGATCTTGCAGGCGTGCCATCAAGTCGTCGGACAGGCGTTTATACAGGACGATTCGCTTCTTCATTGCAGGTTTCCTAATGGTTCATGGCCAGACGCGAGCTATCGCGCTTTGCCCGGCCatgagg                                                                                                                              >  2:501893/1‑131 (MQ=255)       cTCGACGCGATCTTGCAGGCGTGCCATCAAGTCGTCGTACAGGCGTTTATACAGGACGATTCGCTTCTTCATTGCAGGTTTCCTAATGGTTCATGGCCAGACGCTATCTATCGCGATTTGCCCGGCCATGAGGTCt                                                                                                                           <  2:505310/136‑1 (MQ=255)       cTCGACGCGATCTTGCAGGCGTGCCATCAAGTCGTCGGACAGGCGTTTATACAGGACGATTCGCTTCTTCATTGCAGGTTTCCTAATGGTTCATGGCCAGACGCGAGCTATCGCGCTTTGCCCGGCCATGAGGTCt                                                                                                                           <  2:940388/136‑1 (MQ=255)       cTCGACGCGATCTTGCAGGCGTGCCATCAAGTCGTCGGACAGGCGTTTATACAGGACGATTCGCTTCTTCATTGCAGGTTTCCTAATGGTTCATGGCCAGACGCGAGCTATCGCGCTTTGCCCGGCCATGAGGTCt                                                                                                                           <  1:944089/136‑1 (MQ=255)       cTCGACGCGATCTTGCAGGCGTGCCATCAAGTCGTCGGACAGGCGTTTATACAGGACGATTCGCTTCTTCATTGCAGGTTTCCTAATGGTTCATGGCCAGACGCGAGCTATCGCGCTTTGCCCGGCCATGAGGTCt                                                                                                                           <  1:62115/136‑1 (MQ=255)       cTCGACGCGATCTTGCAGGCGTGCCATCAAGTCGTCGGACAGGCGTTTATACAGGACGATTCGCTTCTTCATTGCAGGTTTCCTAATGGTTCATGGCCAGACGCGAGCTATCGCGCTTTGCCCGGCCATGAGGTCt                                                                                                                           <  1:80754/136‑1 (MQ=255)          gACGCGATCTTGCAGGCGTGCCATCAAGTCGTCGGACAGGCGTTTATACAGGACGATTCGCTTCTTCATTGCAGGTTTCCTAATGGTTCATGGCCAGACGCGAGCTATCGCGCTTTGCCCGGCCATGAGGTCTggg                                                                                                                        >  1:698367/1‑136 (MQ=255)              cgATCTTGCAGGCGTGCCATCAAGTCGTCGGACAGGCGTTTATACAGGACGATTCGCTTCTTCATTGCAGGTTTCCTAATGGTTCATGGCCAGACGCGAGCTATCGCGCTTTGCCCGGCCATGAGGTCTGGGAAGt                                                                                                                    <  1:565559/136‑1 (MQ=255)              cgATCTTGCAGGCGTGCCATCAAGTCGTCGGACAGGCGTTTATACAGGACGATTCGCTTCTTCATTGCAGGTTTCCTAATGGTTCATGGCCAGACGCGAGCTATCGCGCTTTGCCCGGCCATGAGGTCTGGGAAGt                                                                                                                    <  1:10048/136‑1 (MQ=255)               gATCTTGCAGGCGTGCCATCAAGTCGTCGGACAGGCGTTTATACAGGACGATTCGCTTCTTCATTGCAGGTTTCCTAATGGTTCATGGCCAGACGCGAGCTATCGCGCTTTGCCCGGCCATGAGGTCTGGGAAGTa                                                                                                                   >  2:980416/1‑136 (MQ=255)                   ttGCAGGCGTGCCATCAAGTCGTCTGAGAGGCGTTTATACAGTACGATTCGCTTCTTCATTGCATGTATCCTAATGGTTCATGGCCAGACGCGAGCTATCGCACTTTGCCCGGCCATGAGGTCTGTGAAGTATTGa                                                                                                               <  1:915811/136‑1 (MQ=255)                   ttGCAGGCGTGCCATCAAGTCGTCGGACAGGCGTTTATACAGGACGATTCGCTTCTTCATTGCAGGTTTCCTAATGGTTCATGGCCAGACGCGAGCTATCGCGCTTTGCCCGGCCATGAGGTCTGGGAAGTATTGa                                                                                                               <  1:909665/136‑1 (MQ=255)                   ttGCAGGCGTGCCATCAAGTCGTCGGACAGGCGTTTATACAGGACGATTCGCTTCTTCATTGCAGGTTTCCTAATGGTTCATGGCCAGACGCGAGCTATCGCGCTTTGCCCGGCCATGAGGTCTGGGAAGTATTGa                                                                                                               <  1:133645/136‑1 (MQ=255)                   ttGCAGGCGTGCCATCAAGTCGTCGGACAGGCGTTTATACAGGACGATTCGCTTCTTCATTGCAGGTTTCCTAATGGTTCATGGCCAGACGCGAGCTATCGCGCTTTGCCCGGCCATGAGGTCTGGGAAGTATTGa                                                                                                               <  1:183396/136‑1 (MQ=255)                   ttGCAGGCGTGCCATCAAGTCGTCGGACAGGCGTTTATACAGGACGATTCGCTTCTTCATTGCAGGTTTCCTAATGGTTCATGGCCAGACGCGAGCTATCGCGCTTTGCCCGGCCATGAGGTCTGGGAAGTATTGa                                                                                                               <  1:501893/136‑1 (MQ=255)                              ccATCAAGTCGTCGGACAGGCGTTTATACAGGACGATTCGCTTCTTCATTGCAGGTTTCCTAATGGTTCATGGCCAGACGCGAGCTATCGCGCTTTGCCCGGCCATGAGGTCTGGGAAGTATTGAGCGCAGCGGTa                                                                                                    >  1:407717/1‑136 (MQ=255)                                       cgtcgGACAGGCGTTTATACAGGACGATTCGCTTCTTCATTGCAGGTTTCCTAATGGTTCATGGCCAGACGCGAGCTATCGCGCTTTGCCCGGCCATGAGGTCTGGGAAGTATTGAGCGCAGCGGTAAGCGCCACt                                                                                           <  1:809696/136‑1 (MQ=255)                                               aGGCGTTTATACAGGACGATTCGCTTCTTCATTGCAGGTTTCCTAATGGTTCATGGCCAGACGCGAGCTATCGCGCTTTGCCCGGCCATGAGGTCTGGGAAGTATTGAGCGCAGCGGTAAGCGCCACTGCAGTcag                                                                                   <  1:429058/136‑1 (MQ=255)                                                   gTTTATACAGGACGATTCGCTTCTTCATTGCAGGTTTCCTAATGGTTCATGGCCAGACGCGAGCTATCGCGCTTTGCCCGGCCATGAGGTCTGGGAAGTATTGAGCGCAGCGGTAAGCGCCACTGCAGTCagcagc                                                                               >  1:641005/1‑136 (MQ=255)                                                    tttATACAGGACGATTCGCTTCTTCATTGCAGGTTTCCTAATGGTTCATGGCCAGACGCGAGCTATCGCGCTTTGCCCGGCCATGAGGTCTGGGAAGTATTGAGCGCAGCGGTAAGCGCCACTGCAGTCAGCAgcg                                                                              <  2:483590/136‑1 (MQ=255)                                                    tttATACAGGACGATTCGCTTCTTCATTGCAGGTTTCCTAATGGTTCATGGCCAGACGCGAGCTATCGCGCTTTGCCCGGCCATGAGGTCTGGGAAGTATTGAGCGCAGCGGTAAGCGCCACTGCAGTCAGCAgcg                                                                              <  2:347726/136‑1 (MQ=255)                                                                gATTCGCTTCTTCATTGCAGGTTTCCTAATGGTTCATGGCCAGACGCGAGCTATCGCGCTTTGCCCGGCCATGAGGTCTGGGAAGTATTGAGCGCAGCGGTAAGCGCCACTGCAGTCAGCAGCGCACCACACATGa                                                                  >  2:470546/1‑136 (MQ=255)                                                                gATTCGCTTCTTCATTGCAGGTTTCCTAATGGTTCATGGCCAGACGCGAGCTATCGCGCTTTGCCCGGCCATGAGGTCTGGGAAGTATTGAGCGCAGCGGTAAGCGCCACTGCAGTCAGCAGCGCACCACACATGa                                                                  >  2:956205/1‑136 (MQ=255)                                                                    cGCTTCTTCATTGCAGGTTTCCTAATGGTTCATGGCCAGACGCGAGCTATCGCGCTTTGCCCGGCCATGAGGTCTGGGAAGTATTGAGCGCAGCGGTAAGCGCCACTGCAGTCAGCAGCGCACCACACATGAACAg                                                              >  1:779575/1‑136 (MQ=255)                                                                     gCTTCTTCATTGCAGGTTTCCTAATGGTTCATGGCCAGACGCGAGCTATCGCGCTTTGCCCGGCCATGAGGTCTGGGAAGTATTGAGCGCAGCGGTAAGCGCCACTGCAGTCAGCAGCGCACCACACATGAACAgg                                                             >  1:714761/1‑136 (MQ=255)                                                                       ttcttcATTGCAGGTTTCCTAATGGTTCATGGCCAGACGCGAGCTATCGCGCTTTGCCCGGCCATGAGGTCTGGGAAGTATTGAGCGCAGCGGTAAGCGCCACTGCAGTCAGCAGCGCACCACACATGAACAGGTa                                                           <  1:894493/136‑1 (MQ=255)                                                                                        ccTAATGGTTCATGGCCAGACGCGAGCTATCGCGCTTTGCCCGGCCATGAGGTCTGGGAAGTATTGAGCGCAGCGGTAAGCGCCACTGCAGTCAGCAGCGCACCACACATGAACAGGTTAGAGGCGCCGGgcccgc                                          <  2:698367/136‑1 (MQ=255)                                                                                        ccTAATGGTTCATGGCCAGACGCGAGCTATCGCGCTTTGCCCGGCCATGAGGTCTGGGAAGTATTGAGCGCAGCGGAAAGCGCCACTGCAGTCAGCAGCGCACCACACATGAACAGGTAAGAGGCGCCGGgcccgc                                          <  1:470546/136‑1 (MQ=255)                                                                                           aaTGGTTCATGGCCAGACGCGAGCTATCGCGCTTTGCCCGGCCATGAGGTCTGGGAAGTATTGAGCGCAGCGGTAAGCGCCACTGCAGTCAGCAGCGCACCACACATGAACAGGTAAGAGGCGCCGGGcccgcccg                                       <  1:779579/136‑1 (MQ=255)                                                                                                     ggCCAGACGCGAGCTATCGCGCTTTGCCCGTCCATGAGGTCTGGGAAGTATTGAGCGCAGCGGTAAGCGCCACTGCAGTCAGCAGCGCACCACACATGAACAGGTAAGAGGCGCCGGGCCCGCCCGTAGCCCCAtt                             >  1:947549/1‑136 (MQ=255)                                                                                                     ggCCAGACGCGAGCTATCGCGCTTTGCCCGGCCATGAGGTCTGGGAAGTATTGAGCGCAGCGGTAAGCGCCACTGCAGTCAGCAGCGCACCACACATGAACAGGTAAGAGGCGCCGGGCCCGCCCGTAGCCCCAtt                             >  2:678974/1‑136 (MQ=255)                                                                                                     ggCCAGACGCGAGCTATCGCGCTTTGCCCGGCCATGAGGTCTGGGAAGTATTGAGCGCAGCGGTAAGCGCCACTGCAGTCAGCAGCGCACCACACATGAACAGGTAAGAGGCGCCGGGCCCGCCCGTAGCCCCAtt                             >  2:738087/1‑136 (MQ=255)                                                                                                     ggCCAGACGCGAGCTATCGCGCTTTGCCCGGCCATGAGGTCTGGGAAGTATTGAGCGCAGCGGTAAGCGCCACTGCAGTCAGCAGCGCACCACACATGAACAGGTAAGAGGCGCCGGGCCCGCCCGTAGCCCCAtt                             >  2:926895/1‑136 (MQ=255)                                                                                                             gcgAGCTATCGCGCTTTGCCCGGCCATGAGGTCTGGGAAGTATTGAGCGCAGCGGTAAGCGCCACTGCAGTCAGCAGCGCACCACACATGAACAGGTAAGAGGCGCCGGGCCCGCCCGTAGCCCCATTGAGGTAGc                     <  2:888794/136‑1 (MQ=255)                                                                                                                  cTATCGCGCTTTGCCCGGCCATGAGGTCTGGGAAGTATTGAGCGCAGCGGTAAGCGCCACTGCAGTCAGCAGCGCACCACACATGAACAGGTAAGAGGCGCCGGGCCCGCCCGTAGCCCCATTGAGGTAGCccacc                >  2:350442/1‑136 (MQ=255)                                                                                                                   tATCGCGCTTTGCCCGGCCATGAGGTCTGGGAAGTATTGAGCGCAGCGGTAAGCGCCACTGCAGTCAGCAGCGCACCACACATGAACAGGTAAGAGGCGCCGGGCCCGCCCGTAGCCCCATTGAGGTAGCccacca               >  2:606263/1‑136 (MQ=255)                                                                                                                                ccGGCCATGAGGTCTGGGAAGTATTGAGCGCAGCGGTAAGCGCCACTGCAGTCAGCAGCGCACCACACATGAACAGGTAAGAGGCGCCGGGCCCGCCCGTAGCCCCATTGAGGTAGCCCACCAGCCACGAgccgcc  >  2:616753/1‑136 (MQ=255)                                                                                                                                       |                                                                                                                                 GGTCACCTCGACGCGATCTTGCAGGCGTGCCATCAAGTCGTCGGACAGGCGTTTATACAGGACGATTCGCTTCTTCATTGCAGGTTTCCTAATGGTTCATGGCCAGACGCGAGCTATCGCGCTTTGCCCGGCCA‑GAGGTCTGGGAAGTATTGAGCGCAGCGGTAAGCGCCACTGCAGTCAGCAGCGCACCACACATGAACAGGTAAGAGGCGCCGGGCCCGCCCGTAGCCCCATTGAGGTAGCCCACCAGCCACGAGCCGCC  >  NC_002947/3823768‑3824029

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑