Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	NC_002947	1,843,229	(GCGCCAGTTCGTGGC)1→2	coding (1607/2754 nt)	gacS ←	sensor protein GacS

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_002947	1843211 =	4 (0.100)	22 (0.580)	16/256	0.6	78.7%	coding (1625/2754 nt)	gacS	sensor protein GacS
	?	NC_002947	= 1843225	8 (0.210)					coding (1611/2754 nt)	gacS	sensor protein GacS

CCCGGGCGAAGGTTCGCAGTTCTGGATCAGCCTCAACCTGCCCAAGGCTCATGACGATGCCGAAGCGCTGCCACCGCAGCCATTGCTGGGCCGCCGTGCCGCCATTGTCGATGGCCACGAACTGGCGCGCCA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_002947/1843342‑1843211 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gccaCGAACTGGCGCGCCAGGCACTGGAGCACCAGCTGGAAGACTGCGGCCTCAGCGTGAGCCTGTTCGCCTCCTACGACCAGTTGCTGCAGGGGGTGCAGGCCGCCAGCCAGGCCGGCCTGCCCTTCGAGTTC  <  NC_002947/1843225‑1843096                                                                                                                                                                                                                                                                        CCCGGGCGAAGGTTCGCAGTTCTGGATCAGCCTCAACCTGCCCAAGGCTCATGACGATGCCGAAGCGCTGCCACCGCAGCCATTGCTGGGCCGCCGTGCCGCCATTGTCGATGGCCACGAACTGGCGCGCCACGca                                                                                                                                >  2:525782/1‑134    GGGCGAAGGTTCGCAGTTCTGGATCAGCCTCAACCTGCCCAAGGCTCATGACGATGCCGAAGCGCTGCCACCGCAGCCATTGCTGGGCCGCCGTGCCGCCATTGTCGATGGCCACGAACTGGCGCGCCACGAACTG                                                                                                                             >  1:229536/1‑136      GCGAAGGTTCGCAGTTCTGGATCAGCCTCAACCTGCCCAAGGCTCATGACGATGCCGAAGCGCTGCCACCGCAGCCATTGCTGGGCCGCCGTGCCGCCATTGTCGATGGCCACGAACTGGCGCGCCACGCACAGGC                                                                                                                           >  2:603304/1‑136      GCGAAGGTTCGCAGTTCTGGATCAGCCTCAACCTGCCCAAGGCTCATGACGATGCCGAAGCGCTGCCACCGCAGCCATTGCTGGGCCGCCGTGCCGCCATTGTCGATGGCCACGAACTGGCGCGCCACGAACTGGC                                                                                                                           >  1:572673/1‑136        GAAGGTTCGCAGTTCTGGATCAGCCTCAACCTGCCCAAGGCTCATGACGATGCCGAAGCGCTGCCACCGCAGCCATTGCTGGGCAGCCGTGCCGCCATTGTCGATGGCCACGAACTGGCGCGCCCCGCACTGGCGC                                                                                                                         >  1:684961/1‑136           GGTTCGCAGTTCTGGATCAGCCTCAACCTGCCCAAGGCTCATGACGATGCCGAAGCGCTGCCACCGCAGCCATTGCTGGGCCGCCGTGCCGCCATTGTCGATGGCCACGAACTGGCGCGCCCCGAACTGGCGCGCC                                                                                                                      >  2:868173/1‑136            GTTCGCAGTTCTGGATCAGCCTCAACCTGCCCAAGGCTCATGACGATGCCGAAGCGCTGCCACCGCAGCCATTGCTGGGCCGCCGTGCCGCCATTGTCGATGGCCACGAACTGGCGCCCCACCAACTGGCGCGCCc                                                                                                                     >  1:337559/1‑135                                 TCAACCTGCCCAAGGCTCATGACGATGCCGAAGCGCTGCCACCGCAGCCATTGCTGGGCCGCCGTGCCGCCATTGTCGATGGCCACGAACTGGCGCGCCACGAACTGGCGCGCCAGGCACTGGAGCACCAGCTGGA                                                                                                >  1:654416/1‑136                                 TCAACCTGCCCAAGGCTCATGACGATGCCGAAGCGCTGCCACCGCAGCCATTGCTGGGCCGCCGTGCCGCCATTGTCGATGGCCACGAACTGGCCCGCCACGAACTGGCGCGCCAGGCCCAGGAGCACCAccgggg                                                                                                >  1:209069/1‑130                                            AAGGCTCATGACGATGCCGAAGCGCTGCCACCGCAGCCATTGCTGGGCCGCCGTGCCGCCATTGTCGATGGCCACGAACTGGCGCGCCACGAACTGGCGCGCCAGGCACTGGAGCACCAGCTGGAAGACTGCGGCC                                                                                     >  2:494834/1‑136                                            AAGGCTCATGACGATGCCGAAGCGCTGCCACCGCAGCCATTGCTGGGCCGCCGTGCCGCCATTGTCGATGGCCACGAACTGGCGCGCCACGAACAGGCGCGCCAGGCACTGGAGCACCAGCTGGAAGACTGCGGCC                                                                                     >  1:547787/1‑136                                            AAGGCTCATGACGATGCCGAAGCGCTGCCACCGCAGCCATTGCTGGGCCGCCGTGCCGCCATTGTCGATGGCCACGAACTGGCGCGCCACCCACAGGCGCGCCAGGCACAGGAGCACCACCTTGAACACAGCGGCC                                                                                     >  2:815709/1‑136                                            AAGGCTCATGACGATGCCGAAGCGCTGCCACCGCAGCCATTGCTGGGCCGCCGTGCCGCCATTGTCGATGGCCACGAACTGGCGCCCCACGAACTGGCGCGCCAGGCACAGGAGCACCAGCAGGAAGACTGCCGCC                                                                                     >  2:171516/1‑136                                                      ACGATGCCGAAGCGCTGCCACCGCAGCCATTGCTGGGCCGCCGTGCCGCCATTGTCGATGGCCACGAACTGGCGCGCCCCGAACAGGCGCGCCCGGCCCAGGAGCCCCCGCAGGAAGACTGCGGCCGCCGCGTGAG                                                                           >  1:401072/1‑136                                                           gcctatGCGCTGCCACCGCAGCCTTTGCTGGGCCGCCGTGCCGCCTTTGTCGATGGCCACTAACGGGCGCGCCACTAACTGGCGCGCCAGGCACTGGAGCACCAGCTGGAAGACTGCGGCCTCAGCGTGAGCCTGT                                                                      <  2:229536/130‑1                                                             CGAAGCGCTGCCACCGCAGCCATTGCTGGGCCGCCGTGCCGCCATTGTCGATGGCCACGAACTGGCGCGCCACGAACTGGCGCGCCAGGCACTGGAGCACCAGCTGGAAGACTGCGGCCTCAGCGTGAGCCTGTTg                                                                    >  1:575440/1‑135                                                                  CGCTGCCACCGCAGCCATTGCTGGGCCGCCGTGCCGCCATTGTCGATGGCCACGAACTGGCGCGCCACGAACTGGCGCGCCAGGCACTGGAGCACCAGCTGGAAGACTGCGGCCTCAGCGTGAGCCTGTTAGCCTC                                                               >  2:561010/1‑136                                                                                                        atcggCGATGCCCACGAAGGGGCCCGCCCCGATCTGGCGCGCCAGGCACTGGAGCACCAGCTGGAAGACTGCGGCCTCAGCGTGAGCCTGTTCGCCTCCTACGACCAGTTGCTGCAGGGGGTGCAGGCCGCCAGCC                         <  2:822216/131‑1                                                                                                            TCGATGGCCACGAACTGGCGCGCCACGAACTGGCGCGCCAGGCACTGGAGCACCAGCTGGAAGACTGCGGCCTCAGCGTGAGCCTGTTCGCCTCCTACGACCAGTTGCTGCAGGGGGTGCCGGCCGCCCGCCCGGC                     >  2:361310/1‑136                                                                                                                             cCGCCCCACCACCGGGCGCCCCAGGCACTGGAGCACCAGCTGGAAGACTGCGGCCTCAGCGTGAGCCTGTTCGCCTCCTACGACCAGTTGCTGCAGGGGGTGCAGGCCGCCAGCCAGGCCGGCCTGCCCTTCGAGT    <  2:572673/135‑1                                                                                                                               GCGCCACTACCTGGCGCGCCAGGCACTGGAGCACCAGCTGGAAGACTGCGGCCTCAGCGTGAGCCTGTTCGCCTCCTACGACCAGTTGCTGCAGGGGGTGCAGGCCGCCAGCCAGGCCGGCCGGCCCTTCGAGTTC  <  2:951022/136‑1                                                                                                                               GCGCCACGAACTGGCGCGCCAGGCACTGGAGCACCAGCTGGAAGACTGCGGCCTCAGCGTGAGCCTGTTCGCCTCCTACGACCAGTTGCTGCAGGGGGTGCAGGCCGCCAGCCAGGCCGGCCTGCCCTTCGAGTTC  >  1:940305/1‑136                                                                                                                                                                                                                                                                        CCCGGGCGAAGGTTCGCAGTTCTGGATCAGCCTCAACCTGCCCAAGGCTCATGACGATGCCGAAGCGCTGCCACCGCAGCCATTGCTGGGCCGCCGTGCCGCCATTGTCGATGGCCACGAACTGGCGCGCCA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_002947/1843342‑1843211 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gccaCGAACTGGCGCGCCAGGCACTGGAGCACCAGCTGGAAGACTGCGGCCTCAGCGTGAGCCTGTTCGCCTCCTACGACCAGTTGCTGCAGGGGGTGCAGGCCGCCAGCCAGGCCGGCCTGCCCTTCGAGTTC  <  NC_002947/1843225‑1843096

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.