Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 1,484,303 T→G V125G (GTC→GGC)  yhdX → amino acid ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,484,3030TG97.5% 142.6 / NA 41V125G (GTC→GGC) yhdXamino acid ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base T (0/1);  major base G (17/22);  minor base C (1/0);  total (18/23)
Fisher's exact test for biased strand distribution p-value = 4.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCGTCATCGGCCTGCTCAATACGCTGTTGGTGACCTTCATCGGCGTCATTCTGGCGACGATACTGGGCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGTCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCGTGTTCCTGACCCTGCCGGGACCGCGGGGCAGCATCAACATCGAGGACATGTTCTTCATCAGCAACCGT  >  NC_002947/1484176‑1484433
                                                                                                                               |                                                                                                                                  
ttCGTCATCGGCCTGCTCAATACGCTGTTGGTGACCTTCATCGGCGTCATTCTGGCGACGATACTGGGCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTgg                                                                                                                            >  1:332221/1‑136 (MQ=255)
ttCGTCATCGGCCTGCTCAATACGCTGTTGGTGACCTTCATCGGCGTCATTCTGGCGACGATACTGGGCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTgg                                                                                                                            >  1:95323/1‑136 (MQ=255)
ttCGTCATCGGACTGCTCAATACGCTGTTGGTGACCTTCATCGGCGTCATTCTGGCGACGATACTGGGCTTCATCATCGGTGTGGCACGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGCCTATGTgg                                                                                                                            >  1:144122/1‑136 (MQ=255)
         ggCCTGCTCAATACGCTGTTGGTGACCTTCATCGGCGTCATTCTGGCGACGATACTGGGCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTcc                                                                                                                   <  2:5315/136‑1 (MQ=255)
          gCCTGCTCAATACGCTGTTGGTGACCTTCATCGGCGTCATTCTGGCGACGATACTGGGCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCg                                                                                                                  >  2:926389/1‑136 (MQ=255)
          gCCTGCTCAATACGCTGTTGGTGACCTTCATCGGCGTCATTCTGGCGACGATACTGGGCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCg                                                                                                                  >  1:261176/1‑136 (MQ=255)
          gCCTGCTCAATACCCTGTTGGTGACCTTCATCCGCGTCATTCTGGCGACGATAATGGGCTTCATCATCGGTGTGTCACGACTGTCACCGAACAGCATGATCAAAAATCTGGCGACCGGCAATGTGGAAAACATccc                                                                                                                  >  2:911867/1‑135 (MQ=255)
           ccTGCTCAATACGCTGTTGGTGACCTTCATCGGCGTCATTCTGGCGACGATACTGGGCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGc                                                                                                                 >  1:646274/1‑136 (MQ=255)
                  aaTACGCTGTTGGTGACCTTCATCGGCGTCATTCTGGCGACGATACTGGGCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTc                                                                                                          <  1:291163/136‑1 (MQ=255)
                      cGCTGTTGGTGACCTTCATCGGCGTCATTCTGGCGACGATACTGGGCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTccgcc                                                                                                      <  2:507334/136‑1 (MQ=255)
                      cGCTGTTGGTGACCTTCATCGGCGTCATTCTGGCGACGATACTGGGCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTccgcc                                                                                                      <  2:529957/136‑1 (MQ=255)
                      cGCTGTTGGTGACCTTCATCGGCGTCATACTGGCGACGATAGTGGGCTTCATCATCGGTGTGGCTCGCCGGTCCCCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTccgcc                                                                                                      <  2:341445/136‑1 (MQ=255)
                       gCTGTTGGTGACCTTCATCGGCGTCATTCTGGCGACGATACTGGGCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTc                                                                                                          >  1:565919/1‑131 (MQ=255)
                       gCTGTTGGTGACCTTCATCGGCGTCATTCTGGCGACGATACTGGGCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTc                                                                                                          <  2:565919/131‑1 (MQ=255)
                           ttGGTGTACTTCATCGTCGTCATTCTGGCGAGGTTACTGGGATTCATCTTCGTTGTGGCTCGCCTGTCACCGAACTGGATTATAAAATTTCTGGCGACCGGCTATGTGGAAACATTCCGCAACATTCCGCCgtttt                                                                                                 <  1:336811/136‑3 (MQ=255)
                            tGGTGACCTTCATCGGCGTCATTCTGGCGACGATACTGGGCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCgttgtt                                                                                                >  2:702744/1‑136 (MQ=255)
                            tGGTGACCTTCATCGGCGTCATTCTGGCGACGATACTGGGCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCgttgtt                                                                                                >  2:329687/1‑136 (MQ=255)
                             ggTGACCTTCATCGGCGTCATTCTGGCGACGATACTGGGCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGttgttg                                                                                               >  2:641061/1‑136 (MQ=255)
                              gTGACCTTCATCGGCGTCATTCTGGCGACGATACTGGGCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGc                                                                                              <  2:253130/136‑1 (MQ=255)
                               tGACCTTCATCGGCGTCATTCTGGCGACGATACTGGGCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCa                                                                                             >  1:339166/1‑136 (MQ=255)
                                 aCCTTCATCGGCGTCATTCTGGCGACGATACTGGGCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGa                                                                                           >  2:599065/1‑136 (MQ=255)
                                                aTTCTGGCGACGATACTGGGCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTAtt                                                                            <  1:491917/136‑1 (MQ=255)
                                                aTTCTGGCGACGATACTGGGCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTAtt                                                                            <  2:173584/136‑1 (MQ=255)
                                                  tCTGGCGACGATACTGGGTTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTc                                                                          <  1:265010/136‑1 (MQ=255)
                                                  tCTGGCGACGATACTGGGCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTc                                                                          <  2:646274/136‑1 (MQ=255)
                                                  tCTGGCGACGATACTGGGCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTc                                                                          <  2:381531/136‑1 (MQ=255)
                                                  tCTGGCGACGATACTGGGCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTc                                                                          <  2:356891/136‑1 (MQ=255)
                                                  tCTGGCGACGATACTGGGCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTc                                                                          <  2:541263/136‑1 (MQ=255)
                                                                     ttCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGTCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCGTGTTCCTGACCCTGc                                                       <  2:217666/136‑1 (MQ=255)
                                                                          catcGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCGTGTTCCTGACCCTGCCGGGa                                                  >  1:57714/1‑136 (MQ=255)
                                                                                tgtgGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCGTGTTCCTGACCCTGCCGGGACCGCgg                                            <  1:641061/136‑1 (MQ=255)
                                                                                ggtgGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCGTGTTCCTGACCCTGCCGGGACCGCgg                                            <  1:901853/135‑1 (MQ=255)
                                                                                       cGCCTGTCACCTAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCGTGTTCCTGACCCTGCCGGGACCGCGGGgcagca                                     <  2:339166/136‑1 (MQ=255)
                                                                                        gCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCGTGTTCCTGACCCTGCCGGGACCGCGGGGCAGCAt                                    <  1:875427/136‑1 (MQ=255)
                                                                                        gCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCGTGTTCCTGACCCTGCCGGGACCGCGGGGCAGCAt                                    <  1:208220/136‑1 (MQ=255)
                                                                                          cTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCGTGTTCCTGACCCTGCCGGGACCGCGGGGCAGCATCa                                  <  2:332221/136‑1 (MQ=255)
                                                                                            gTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCGTGTTCCTGACCCTGCCGGGACCGCGGGGCAGCATCAAc                                >  2:699276/1‑136 (MQ=255)
                                                                                                  gAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCGTGTTCCTGACCCTGCCGGGACCGCGGGGCAGCATCAACATCGAg                          >  2:207985/1‑136 (MQ=255)
                                                                                                               aacaaGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCGTGTTCCTGACCCTGCCGGGACCGCGGGGCAGCATCAACATCGAGGACATGTTCTtca             <  2:216243/136‑1 (MQ=255)
                                                                                                                     cTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCGTGTTCCTGACCCTGCCGGGACCGCGGGGCAGCATCAACATCGAGGACATGTTCTTCATCAGCa       >  1:319566/1‑136 (MQ=255)
                                                                                                                          gACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCGTGTTCCTGACCCTGCCGGGACCGCGGGGCAGCATCAACATCGAGGACATGTTCTTCATCAGCAACCGt  >  2:539708/1‑136 (MQ=255)
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TTCGTCATCGGCCTGCTCAATACGCTGTTGGTGACCTTCATCGGCGTCATTCTGGCGACGATACTGGGCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGTCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCGTGTTCCTGACCCTGCCGGGACCGCGGGGCAGCATCAACATCGAGGACATGTTCTTCATCAGCAACCGT  >  NC_002947/1484176‑1484433

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑