Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	3,824,415	A→G	I245T (ATC→ACC)	kguT ←	2‑ketogluconate transporter, putative

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,824,415	0	A	G	100.0%	105.5 / NA	29	I245T (ATC→ACC)	kguT	2‑ketogluconate transporter, putative
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/14);  total (15/14)

CAGGACACGCCCACCATTGCCAGCACCGCCGCCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAGCCACAGCACGAAGCCATACACGCCGATGCTCCAGCAGAAGTACTGCAGCGCCAGGATGATCACTTGCGGCGAGCGGAACGCTTCGCGGTAGTTCTTTACAGGCTTGATACCCTGCTGCTCGGCAGCCAGCGCCTGCGCCAGGGCGGCCTTTTCCTGC  >  NC_002947/3824283‑3824546                                                                                                                                     |                                                                                                                                    cAGGACACGCCCACCATTGCCAGCACCGCCGCCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAGCCACAGCACGAAGCCATACACGCCGGTGc                                                                                                                                  <  2:790173/136‑1 (MQ=255) cAGGACACGCCCACCATTGCCAGCACCGCCGCCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAGCCACAGCACGAAGCCATACACGCCGGTGc                                                                                                                                  >  2:189655/1‑136 (MQ=255)    gACACGCCCACCATTGCCAGCACCGCCGCCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAGCCACAGCACGAAGCCATACACGCCGGTGCTcc                                                                                                                               <  1:122934/136‑1 (MQ=255)             accaTTGCCAGCACCGCCGCCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAGCCACAGCACGAAGCCATACACGCCGGTGCTCCAGCAGAAGt                                                                                                                      >  2:101922/1‑136 (MQ=255)              ccaTTGCCAGCACCGCCGCCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAGCCACAGCACGAAGCCATACACGCCGGTGCTCCAGCAGAAGTa                                                                                                                     <  1:568416/136‑1 (MQ=255)                 ttGCCAGCACCGCCGCCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAGCCACAGCACGAAGCCATACACGCCGGTGCTCCAGCAGAAGTACTg                                                                                                                  >  1:367294/1‑136 (MQ=255)                     cAGCACCGCCGCCTGGTAAGGCACCGAGGCCAGCCCGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAGCCACAGCACGAAGCCATACACGCCGGTGCTCCAGCAGAAGTACTGCAgc                                                                                                              <  1:779691/136‑1 (MQ=255)                                ccAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAGCCACAGCACGAAGCCATACACGCCGGTGCTCCAGCAGAAGTACTGCAGCGCCAGgatgat                                                                                                   >  2:36108/1‑136 (MQ=255)                                                 gccagccaGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAGCCACAGCACGAAGCCATACACGCCGGTGCTCCAGCAGAAGTACTGCAGCGCCAGGATGATCACTTGCGGCGAGCGGa                                                                                  >  2:74148/1‑136 (MQ=255)                                                  ccagccagCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAGCCACAGCACGAAGCCATACACGCCGGTGCTCCAGCAGAAGTACTGCAGCGCCAGGATGATCACTTGCGGCGAGCGGaa                                                                                 >  2:72279/1‑136 (MQ=255)                                                         gccGGCCTGCACGATATCGCCGTTCGCCGCCTGCTTGAGGATCGACGGCAGCCACAGCACGAAGCCAAACACGCCGGTGCTCCAGCAGAAGTACTGCAGCGCCAGGATGATCACTTGCGGCGAGCGGAACGCTTcg                                                                          <  1:321096/136‑1 (MQ=255)                                                         cccGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAGCCACAGCACGAAGCCATCCACGCCGGTGCTCCAGCAGAAGTACTGCAGCGCCAGGATGATCACTTGCGGCGAGCGGAACGCTTcg                                                                          <  1:661121/135‑1 (MQ=255)                                                         cccGCCCTGCAGTATATCGACTTTCGCCGCTTGCTTGAGTATCCACGGCACCCACACCACTAACCCATACACGCCGGTGCTCCAGCAGAAGTACTGCAGCGCCAGGATGATCACTTGCGGCGAGCGGAACGCTTcg                                                                          <  2:972368/135‑1 (MQ=255)                                                           cGGCCTGCACTATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAGCCACAGCACGAAGCCATACACGCCGGTGCTCCAGCAGAAGTACTGCAGCGCCAGGATGATCACTTGCGGCGAGCGGAACGCTTcgcg                                                                        <  2:536970/136‑1 (MQ=255)                                                           cGGCCTGCACCAAATCGACGTCCGCCGCCTGCTTGAGGCCCCCCGGCAGCCACACCACGAACCAAAACACGCCGGGGCTCCAGCAGAAGTACTGCAGCGCCAGGATGATCACTTGCGGCGAGCGGAACGCTTcgcg                                                                        <  1:377200/136‑1 (MQ=255)                                                                          cGACGTTCGCCGCCTGCTTGAGGATCGACGGCAGCCACAGCACGAAGCCATACACGCCGGTGCTCCAGCAGAAGTACTGCAGCGCCAGGATGATCACTTGCGGCGAGCGGAACGCTTcg                                                                          <  1:822300/119‑1 (MQ=255)                                                                          cGACGTTCGCCGCCTGCTTGAGGATCGACGGCAGCCACAGCACGAAGCCATACACGCCGGTGCTCCAGCAGAAGTACTGCAGCGCCAGGATGATCACTTGCGGCGAGCGGAACGCTTcg                                                                          >  2:822300/1‑119 (MQ=255)                                                                               ttCGCCGCCTGCTTGAGGATCGACGGCAGCCACAGCACGAAGCCATACACGCCGGTGCTCCAGCAGAAGTACTGCAGCGCCAGGATGATCACTTGCGGCGAGCGGAACGCTTCGCGGTAGTTCTTTACAGGCTTGa                                                    >  2:343538/1‑136 (MQ=255)                                                                               ttCGCCGCCTGCTTGAGGATCGACGGCAGCCACAGCACGAAGCCATACACGCCGGTGCTCCAGCAGAAGTACTGCAGCGCCAGGATGATCACTTGCGGCGAGCGGAACGCTTCGCGGTAGTTCTTTACAGGCTTGa                                                    >  1:329185/1‑136 (MQ=255)                                                                                           ttGAGGATCGCCGGCAGCCACAGCACGAAGCCATCCACGCCGGTGCTCCAGCAGAAGTACTGCAGCGCCAGGATGATCACTTGCGGCGAGCGGAACGCTTCGCGGTAGTTCTTTACAGGCTTGATACCCTgctgct                                        <  2:641005/136‑1 (MQ=255)                                                                                                       ggCAGCCACAGCACGAAGCCATACACGCCGGTGCTCCAGCAGAAGTACTGCAGCGCCAGGATGATCACTTGAGGCGAGCGGAACGCTTCGCGGTAGTTCTTTACAGGCTTGATACCCTGCTGCTCGGCAGCCAgcg                            >  1:540829/1‑136 (MQ=255)                                                                                                                   aCGAAGCCATACACGCCGGTGCTCCAGCAGAAGTACTGCAGCGCCAGGATGATCACTTGCGGCGAGCGGAACGCTTCGCGGTAGTTCTTTACAGGCTTGATACCCTGCTGCTCGGCAGCCAGCGCCTGCGCCAggg                >  1:291010/1‑136 (MQ=255)                                                                                                                          cATACACGCCGGTGCTCCAGCAGAAGTACTGCAGCGCCAGGATGATCACTTGCGGCGAGCGGAACGCTTCGCGGTAGTTCTTTACAGGCTTGATACCCTGCTGCTCGGCAGCCAGCGCCTGCGCCAGGGCGGCCtt         >  1:564548/1‑136 (MQ=255)                                                                                                                          cATACACGCCGGTGCTCCAGCAGAAGTACTGCAGCGCCAGGATGATCACTTGCGGCGAGCGGAACGCTTCGCGGTAGTTCTTTACAGGCTTGATACCCTGCTGCTCGGCAGCCAGCGCCTGCGCCAGGGCGGCCtt         >  2:83575/1‑136 (MQ=255)                                                                                                                             acacGCCGGTGCCCCAGCAGAAGTACTGCAGCGCCAGGATGATCACTTGCGGCGAGCGGAACGCTTCGCGGTAGTTCTTTACAGGCTTGATACCCTGCTGCTCGGCAGCCAGCGCCTGCGCCAGGGCGGCCTTTTc      <  2:561210/136‑1 (MQ=255)                                                                                                                               acGCCGGTGCTCCAGCAGAAGTACTGCAGCGCCAGGATGATCACTTGCGGCGAGCGGAACGCTTCGCGGTAGTTCTTTACAGGCTTGATACCCTGCTGCTCGGCAGCCAGCGCCTGCGCCAGGGCGGCCTTTTCCt    >  1:641555/1‑136 (MQ=255)                                                                                                                               acGCCGGTGCTCCAGCAGAAGTACTGCAGCGCCAGGATGATCACTTGCGGCGAGCGGAACGCTTCGCGGTAGTTCTTTACAGGCTTGATACCCTGCTGCTCGGCAGCCAGCGCCTGCGCCAGGGCGGCCTTTTCCt    >  2:717506/1‑136 (MQ=255)                                                                                                                                 gCCGGTGCTCCAGCAGAAGTACTGCAGCGCCAGGATGATCACTTGCGGCGAGCGGAACGCTTCGCGGTAGTTCTTTACAGGCTTGATACCCTGCTGCTCGGCAGCCAGCGCCTGCGCCAGGGCGGCCTTTTCCTgc  <  1:72279/136‑1 (MQ=255)                                                                                                                                 gCCGGTGCTCCAGCAGAAGTACTGCAGCGCCAGGATGATCACTTGCGGCGAGCGGAACGCTTCGCGGTAGTTCTTTACAGGCTTGATACCCTGCTGCTCGGCAGCCAGCGCCTGCGCCAGGGCGGCCTTTTCCTgc  <  2:367294/136‑1 (MQ=255)                                                                                                                                     |                                                                                                                                    CAGGACACGCCCACCATTGCCAGCACCGCCGCCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAGCCACAGCACGAAGCCATACACGCCGATGCTCCAGCAGAAGTACTGCAGCGCCAGGATGATCACTTGCGGCGAGCGGAACGCTTCGCGGTAGTTCTTTACAGGCTTGATACCCTGCTGCTCGGCAGCCAGCGCCTGCGCCAGGGCGGCCTTTTCCTGC  >  NC_002947/3824283‑3824546

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑