Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 315,243 G→T W19L (TGG→TTG)  cysQ → 3'(2'),5'‑bisphosphate nucleotidase CysQ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947315,2430GT99.4% 1339.6 / NA 358W19L (TGG→TTG) cysQ3'(2'),5'‑bisphosphate nucleotidase CysQ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (180/176);  minor base A (2/0);  total (182/176)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 358 total aligned reads displayed.

TCACCGAGGGCCGCGCCCTGGCGGCGCTGTACCTGGCCCGTGACCTGCTGATTCAGCGGGGGCTGCTGGAGCTATGAACGACCTGCAACTGATGCATGAAGTGGTCAAGCTGGCGCTGACGGCAGGCGAGGCGATCCTGCCATTCTGGCGCGCCGATGTCGCGGTAACCAACAAGGCCGACGATTCGCCGGTTACCGCCGCCGACCTAGCCGCGCACCGGGTAATCGCCGATGGTCTGCTGGCGCTGGCGCCGCAGATTCCGGTGCTGTCCGAAGAGGACTGCAATATTGC  >  NC_002947/315097‑315387
                                                                                                                                                  |                                                                                                                                                
tCACCGAGGGCCGCGCCCTGGCGGCGCTGTACCTGGCCCGTGACCTGCTGATTCAGCGGGGGCTGCTGGAGCTATGAACGACCTGCAACTGATGCATGAAGTGGTCAAGCTGGCGCTGACGGCAGGCGAGGCGATCCTGCCCTTCTTgc                                                                                                                                                >  2:1197150/1‑149 (MQ=255)
tCACCGAGGGCCGCGCCCTGGCGGCGCTGTACCTGGCCCGTGACCTGCTGATTCAGCGGGGGCTGCTGGAGCTATGAACGACCTGCAACTGATGCATGAAGTGGTCAAGCTGGCGCTGACGGCAGGCGAGGCGATCCTGCCATTCTTgc                                                                                                                                                <  1:5352334/149‑1 (MQ=255)
   ccGAGGGCCGCGCCCTGGCGGCGCTGTACCTGGCCCGTGACCTGCTGATTCAGCGGGGGCTGCTGGAGCTATGAACGACCTGCAACTGATGCATGAAGTGGTCAAGCTGGCGCTGACGGCAGGCGAGGCGATCCTGCCATTCTTgcgcg                                                                                                                                             >  1:2017642/1‑149 (MQ=255)
     gAGGGCCGCGCCCTGGCGGCGCTGTACCTGGCCCGTGACCTGCTGATTCAGCGGGGGCTGCTGGAGCTATGAACGACCTGCAACTGATGCATGAAGTGGTCAAGCTGGCGCTGACGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGcc                                                                                                                                           >  1:1710235/1‑149 (MQ=255)
     gAGGGCCGCGCCCTGGCGGCGCTGTACCTGGCCCGTGACCTGCTGATTCAGCGGGGGCTGCTGGAGCTATGAACGACCTGCAACTGATGCATGAAGTGGTCAAGCTGGCGCTGACGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGcc                                                                                                                                           >  2:1734221/1‑149 (MQ=255)
      aGGGCCGCGCCCTGACGGCGCTGTACCTGGCCCGTGACCTGCTGATTCAGCGGGGGCTGCTGGAGCTATGAACGACCTGCAACTGATGCATGAAGTGGTCAAGCTGGCGCTGACGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCg                                                                                                                                          <  2:1998957/149‑1 (MQ=255)
       gggCCGCGCCCTGGCGGCGCTGTACCTGGCCCGTGACCTGCTGATTCAGCGGGGGCTGCTGGAGCTATGAACGACCTGCAACTGATGCATGAAGTGGTCAAGCTGGCGCTGACGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGa                                                                                                                                         <  2:2576494/149‑1 (MQ=255)
       gggCCGCGCCCTGGCGGCGCTGTACCTGGCCCGTGACCTGCTGATTCAGCGGGGGCTGCTGGAGCTATGAACGACCTGCAACTGATGCATGAAGTGGTCAAGCTGGCGCTGACGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGa                                                                                                                                         >  1:3836035/1‑149 (MQ=255)
         gCCGCGCCCTGGCGGCGCTGTACCTGGCCCGTGACCTGCTGATTCAGCGGGGGCTGCTGGAGCTATGAACGACCTGCAACTGATGCATGAAGTGGTCAAGCTGGCGCTGACGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATg                                                                                                                                       >  2:5801530/1‑149 (MQ=255)
           cgcgCCCTGGCGGCGCTGTACCTGGCCCGTGACCTGCTGATTCAGCGGGGGCTGCTGGAGCTATGAACGACCTGCAACTGATGCATGAAGTGGTCAAGCTGGCGCTGACGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTc                                                                                                                                     >  2:881901/1‑149 (MQ=255)
           cgcgCCCTGGCGGCGCTGTACCTGGCCCGTGACCTGCTGATTCAGCGGGGGCTGCTGGAGCTATGAACGACCTGCAACTGATGCATGAAGTGGTCAAGCTGGCGCTGACGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTc                                                                                                                                     >  1:6499633/1‑149 (MQ=255)
           cgcgCCCTGGCGGCGCTGTACCTGGCCCGTGACCTGCTGATTCAGCGGGGGCTGCTGGAGCTATGAACGACCTGCAACTGATGCATGAAGTGGTCAAGCTGGCGCTGACGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTc                                                                                                                                     >  1:4902171/1‑149 (MQ=255)
             cgcCCTGGCGGCGCTGTACCTGGCCCGTGACCTGCTGATTCAGCGGGGGCTGCTGGAGCTATGAACGACCTGCAACTGATGCATGAAGTGGTCAAGCTGGCGCTGACGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTcgc                                                                                                                                   <  1:1208457/149‑1 (MQ=255)
             cgcCCTGGCGGCGCTGTACCTGGCCCGTGACCTGCTGATTCAGCGGGGGCTGCTGGAGCTATGAACGACCTGCAACTGATGCATGAAGTGGTCAAGCTGGCGCTGACGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTcgc                                                                                                                                   <  1:2426243/149‑1 (MQ=255)
               cccTGGCGGCGCTGTACCTGGCCCGTGACCTGCTGATTCAGCGGGGGCTGCTGGAGCTATGAACGACCTGCAACTGATGCATGAAGTGGTCAAGCTGGCGCTGACGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTCGCgg                                                                                                                                 <  2:6378639/149‑1 (MQ=255)
                 cTGGCGGCGCTGTACCTGGCCCGTGACCTGCTGATTCAGCGGGGGCTGCTGGAGCTATGAACGACCTGCAACTGATGCATGAAGTGGTCAAGCTGGCGCTGACGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTCGCGGTa                                                                                                                               <  1:525342/149‑1 (MQ=255)
                 cTGGCGGCGCTGTACCTGGCCCGTGACCTGCTGATTCAGCGGGGGCTGCTGGAGCTATGAACGACCTGCAACTGATGCATGAAGTGGTCAAGCTGGCGCTGACGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTCGCGGTa                                                                                                                               <  2:4566837/149‑1 (MQ=255)
                  tGGCGGCGCTGTACCTGGCCCGTGACCTGCTGATTCAGCGGGGGCTGCTGGAGCTATGAACGACCTGCAACTGATGCATGAAGTGGTCAAGCTGGCGCTGACGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTCGCGGTaa                                                                                                                              <  1:3638380/149‑1 (MQ=255)
                  tGGCGGCGCTGTACCTGGCCCGTGACCTGCTGATTCAGCGGGGGCTGCTGGAGCTATGAACGACCTGCAACTGATGCATGAAGTGGTCAAGCTGGCGCTGACGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTCGCGGTaa                                                                                                                              <  1:6117382/149‑1 (MQ=255)
                   ggcggcGCTGTACCTGGCCCGTGACCTGCTGATTCAGCGGGGGCTGCTGGAGCTATGAACGACCTGCAACTGATGCATGAAGTGGTCAAGCTGGCGCTGACGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTCGCGGTAAc                                                                                                                             <  1:5221708/149‑1 (MQ=255)
                   ggcggcGCTGTACCTGGCCCGTGACCTGCTGATTCAGCGGGGGCTGCTGGAGCTATGAACGACCTGCAACTGATGCATGAAGTGGTCAAGCTGGCGCTGACGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTCGCGGTAAc                                                                                                                             >  2:467656/1‑149 (MQ=255)
                     cggcgCTGTACCTGGCCCGTGACCTGCTGATTCAGCGGGGGCTGTTGGAGCTATGAACGACCTGCAACTGATGCATGAAGTGGTCAAGCTGGCGCTGACGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTCGCGGTAACca                                                                                                                           <  2:6370917/149‑1 (MQ=255)
                     cggcgCTGTACCTGGCCCGTGACCCGCTGATTCAGCGGGGGCTGCTGGAGCTATGAACGACCTGCAACTGATGCATGAAGTGGTCAAGCTGGCGCTGACGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTCGCGGTAACca                                                                                                                           <  1:932429/149‑1 (MQ=255)
                         gcTGTACCTGGCCCGTGACCTGCTGATTCAGCGGGGGCTGCTGGAGCTATGAACGACCTGCAACTGATGCATGAAGTGGTCAAGCTGGCGCTGACGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTCGCGGTAACcaacaa                                                                                                                       <  2:1489426/149‑1 (MQ=255)
                           tGTACCTGGCCCGTGACCTGCTGATTCAGCGGGGGCTGCTGGAGCTATGAACGACCTGCAACTGATGCATGAAGTGGTCAAGCTGGCGCTGACGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTCGCGGTAACCAACAAgg                                                                                                                     <  2:750915/149‑1 (MQ=255)
                              aCCTGGCCCGTGACCTGCTGATTCAGCGGGGGCTGCTGGAGCTATGAACGACCTGCAACTGATGCATGAAGTGGTCAAGCTGGCGCTGACGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTCGCGGTAACCAACAAGGCcg                                                                                                                  <  1:6931539/149‑1 (MQ=255)
                                cTGGCCCGTGACCTGCTGATTCAGCGGGGGCTGCTGGAGCTATGAACGACCTGCAACTGATGCATGAAGTGGTCAAGCTGGCGCTGACGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTCGCGGTAACCAACAAGGCcgac                                                                                                                >  1:1558270/1‑149 (MQ=255)
                                 tGGCCCGTGACCTGCTGATTCAGCGGGGGCTGCTGGAGCTATGAACGACCTGCAACTGATGCATGAAGTGGTCAAGCTGGCGCTGACGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTCGCGGTAACCAACAAGGCcgacg                                                                                                               >  1:2201011/1‑149 (MQ=255)
                                 aggCCCGTGACCTGCTGATTCAGCGGGGGCTGCTGGAGCTATGAACGACCTGAAACTGATGCATGAAGTGGTCAAGCTGGCGCTGACGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTCGCGGTAACCAACAAGGCcgacg                                                                                                               >  1:336869/2‑149 (MQ=255)
                                  ggCCCGTGACCTGCTGATTCAGCGGGGGCTGCTGGAGCTATGAACGACCTGCAACTGATGCATGAAGTGGTCAAGCTGGCGCTGACGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTCGCGGTAACCAACAAGGCcgacga                                                                                                              >  1:6198310/1‑149 (MQ=255)
                                   gCCCGTGACCTGCTGATTCAGCGGGGGCTGCTGGAGCTATGAACGACCTGCAACTGATGCATGAAGTGGTCAAGCTGGCGCTGACGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTCGCGGTAACCAACAAGGCCGACGAt                                                                                                             >  1:3901135/1‑149 (MQ=255)
                                     ccGTGACCTGCTGATTCAGCGGGGGCTGCTGGAGCTATGAACGACCTGCAACTGATGCATGAAGTGGTCAAGCTGGCGCTGACGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTCGCGGTAACCAACAAGGCCGACGATTc                                                                                                           <  1:4401893/149‑1 (MQ=255)
                                      cGTGACCTGCTGATTCAGCGGGGGCTGCTGGAGCTATGAACGACCTGCAACTGATGCATGAAGTGGTCAAGCTGGCGCTGACGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTCGCGGTAACCAACAAGGCCGACGATTCg                                                                                                          >  2:6571292/1‑149 (MQ=255)
                                            ctgctgATTCAGCGGGGGCTGCTGGAGCTATGAACGACCTGCAACTGATGCATGAAGTGGTCAAGCTGGCGCTGACGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTCGCGGTAACCAACAAGGCCGACGATTCGCCGGtt                                                                                                    <  2:4994100/149‑1 (MQ=255)
                                              gctgATTCAGCGGGGGCTGCTGGAGCTATGAACGACCTGCAACTGATGCATGAAGTGGTCAAGCTGGCGCTGACGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTCGCGGTAACCAACAAGGCCGACGATTCGCCGGTTAc                                                                                                  <  1:2672475/149‑1 (MQ=255)
                                              gctgATTCAGCGGGGGCTGCTGGAGCTATGAACGACCTGCAACTGATGCATGAAGTGGTCAAGCTGGCGCTGACGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTCGCGGTAACCAACAAGGCCGACGATTCGCCGGTTAc                                                                                                  >  2:761398/1‑149 (MQ=255)
                                               ctgATTCAGCGGGGGCTGCTGGAGCTATGAACGACCTGCAACTGATGCATGAAGTGGTCAAGCTGGCGCTGACGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTCGCGGTAACCAACAAGGCCGACGATTCGCCGGTTAcc                                                                                                 >  2:410405/1‑149 (MQ=255)
                                                  aTTCAGCGGGGGCTGCTGGAGCTATGAACGACCTGCAACTGATGCATGAAGTGGTCAAGCTGGCGCTGACGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTCGCGGTAACCAACAAGGCCGACGATTCGCCGGTTAccgcc                                                                                              >  2:4485577/1‑149 (MQ=255)
                                                   ttCAGCGGGGGCTGCTGGAGCTATGAACGACCTGCAACTGATGCATGAAGTGGTCAAGCTGGCGCTGACGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTCGCGGTAACCAACAAGGCCGACGATTCGCCGGTTAccgccg                                                                                             >  2:2235226/1‑149 (MQ=255)
                                                     cAGCGGGGGCTGCTGGAGCTATGAACGACCTGCAACTGATGCATGAAGTGGTCAAGCTGGCGCTGACGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTCGCGGTAACCAACAAGGCCGACGATTCGCCGGTTAccgccgcc                                                                                           >  2:4802211/1‑149 (MQ=255)
                                                     cAGCGGGGGCTGCTGGAGCTATGAACGACCTGCAACTGATGCATGAAGTGGTCAAGCTGGCGCTGACGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTCGCGGTAACCAACAAGGCCGACGATTCGCCGGTTAccgccgcc                                                                                           >  2:5283605/1‑149 (MQ=255)
                                                       gCGGGGGCTGCTGGAGCTATGAACGACCAGCAACTGATGCATGAAGTGGTCAAGCTGGCGCTGACGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTCGCGGTAACCAACAAGGCCGACGATTCGCCGGTTACCGCCGCCGa                                                                                         <  1:1935525/149‑1 (MQ=255)
                                                         gggggCTGCTGGAGCTATGAACGACCTGCAACTGATGCATGAAGTGGTCAAGCTGGCGCTGACGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTCGCGGTAACCAACAAGGCCGACGATTCGCCGGTTACCGCCGCCGAcc                                                                                       <  1:5115709/149‑1 (MQ=255)
                                                           gggCTGCTGGAGCTATGAACGACCTGCAACTGATGCATGAAGTGGTCAAGCTGGCGCTGACGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTCGCGGTAACCAACAAGGCCGACGATTCGCCGGTTACCGCCGCCGACCTa                                                                                     >  1:4649286/1‑149 (MQ=255)
                                                               tgctgGAGCTATGAACGACCTGCAACTGATGCATGAAGTGGTCAAGCTGGCGCTGACGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTCGCGGTAACCAACAAGGCCGACGATTCGCCGGTTACCGCCGCCGACCTAGCcg                                                                                 <  1:6981082/149‑1 (MQ=255)
                                                                   ggAGCTATGAACGACCTGCAACTGATGCATGAAGTGGTCAAGCTGGCGCTGACGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTCGCGGTAACCAACAAGGCCGACGATTCGCCGGTTACCGCCGCCGACCTAGCCGCGCa                                                                             >  2:1618070/1‑149 (MQ=255)
                                                                    gAGCTATGAACGACCTGCAACTGATGCATGAAGTGGTCAAGCTGGCGCTGACGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTCGCGGTAACCAACAAGGCCGACGATTCGCCGGTTACCGCCGCCGACCTAGCCGCGCAc                                                                            >  2:1373917/1‑149 (MQ=255)
                                                                     aGCTATGAACGACCTGCAACTGATGCATGAAGTGGTCAAGCTGGCGCTGACGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTCGCGGTAACCAACAAGGCCGACGATTCGCCGGTTACCGCCGCCGACCTAGCCGCGCAcc                                                                           >  2:5163812/1‑149 (MQ=255)
                                                                     aGCTATGAACGACCTGCAACTGATGCATGAAGTGGTCAAGCTGGCGCTGACGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTCGCGGTAACCAACAAGGCCGACGATTCGCCGGTTACCGCCGCCGACCTAGCCGCGCAcc                                                                           >  1:4715056/1‑149 (MQ=255)
                                                                     aGCTATGAACGACCTGCAACTGATGCATGAAGTGGTCAAGCTGGCGCTGACGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTCGCGGTAACCAACAAGGCCGACGATTCGCCGGTTACCGCCGCCGACCTAGCCGCGCAcc                                                                           >  2:4165708/1‑149 (MQ=255)
                                                                      gCTATGCACGACCTGCAACTGATGCATGAAGTGGTCAAGCTGGCGCTGACGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTCGCGGTAACCAACAAGGCCGACGATTCGCCGGTTACCGCCGCCGACCTAGCCGCGCACCg                                                                          <  1:4727508/149‑1 (MQ=255)
                                                                      gCTATGAACGACCTGCAACTGATGCATGAAGTGGTCAAGCTGGCGCTGACGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTCGCGGTAACCAACAAGGCCGACGATTCGCCGGTTACCGCCGCCGACCTAGCCGCGCACCg                                                                          <  1:5013786/149‑1 (MQ=255)
                                                                       cTATGAACGACCTGCAACTGATGCATGAAGTGGTCAAGCTGGCGCTGACGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTCGCGGTAACCAACAAGGCCGACGATTCGCCGGTTACCGCCGCCGACCTAGCCGCGCACCgg                                                                         >  1:6312362/1‑149 (MQ=255)
                                                                           gAACGACCTGCAACTGATGCATGAAGTGGTCAAGCTGGCGCTGACGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTCGCGGTAACCAACAAGGCCGACGATTCGCCGGTTACCGCCGCCGACCTAGCCGCGCACCGGGTaa                                                                     >  1:2806548/1‑149 (MQ=255)
                                                                           gAACGACCTGCAACTGATGCATGAAGTGGCCAAGCTGGCGCTGACGGCAGGCGAGGCAATCCTGCCATTCTTGCGCGCCGATGTCGCGGTAACCAACAAGGCCGACGATTCGCCGGTTACCGCCGCCGACCTAGCCGCGCACCGGGTaa                                                                     >  2:2911860/1‑149 (MQ=255)
                                                                             aCGACCTGCAACTGATGCATGAAGTGGTCAAGCTGGCGCTGACGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTCGCGGTAACCAACAAGGCCGACGATTCGCCGGTTACCGCCGCCGACCTAGCCGCGCACCGGGTAATc                                                                   >  1:5448744/1‑149 (MQ=255)
                                                                             aCGACCTGCAACTGATGCATGAAGTGGTCAAGCTGGCGCTGACGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTCGCGGTAACCAACAAGGCCGACGATTCGCCGGTTACCGCCGCCGACCTAGCCGCGCACCGGGTAATc                                                                   >  1:1402161/1‑149 (MQ=255)
                                                                              cGACCTGCAACTGATGCATGAAGTGGTCAAGCTGGCGCTGACGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTCGCGGTAACCAACAAGGCCGACGATTCGCCGGTTACCGCCGCCGACCTAGCCGCGCACCGGGTAATCg                                                                  <  2:5104971/149‑1 (MQ=255)
                                                                              cGACCTGCAACTGATGCATGAAGTGGTCAAGCTGGCGCTGACGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTCGCGGTAACCAACAAGGCCGACGATTCGCCGGTTACCGCCGCCGACCTAGCCGCGCACCGGGTAATCg                                                                  <  2:3546296/149‑1 (MQ=255)
                                                                                 ccTGCAACTGATGCATGAAGTGGTCAAGCTGGCGCTGACGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTCGCGGTAACCAACAAGGCCGACGATTCGCCGGTTACCGCCGCCGACCTAGCCGCGCACCGGGTAATCGCCg                                                               <  1:3220971/149‑1 (MQ=255)
                                                                                 ccTGCAACTGATGCATGAAGTGGTCAAGCTGGCGCTGACGGAAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTCGCGGTAACCAACAAGGCCGACGATTCGCCGGTTACCGCCGCCGACCTAGCCGCGCACCGGGTAATCGCCg                                                               <  2:3101605/149‑1 (MQ=255)
                                                                                  cTGCAACTGATGCATGAAGTGGTCAAGCTGGCGCTGACGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTCGCGGTAACCAACAAGGCCGACGATTCGCCGGTTACCGCCGCCGACCTAGCCGCGCACCGGGTAATCGCCGa                                                              <  1:1035062/149‑1 (MQ=255)
                                                                                   tGCAACTGATGCATGAAGTGGTCAAGCTGGCGCTGACGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTCGCGGTAACCAACAAGGCCGACGATTCGCCGGTTACCGCCGCCGACCTAGCCGCGCACCGGGTAATCGCCGAt                                                             <  1:4497158/149‑1 (MQ=255)
                                                                                      aaCTGATGCATGAAGTGGTCAAGCTGGCGCTGACGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTCGCGGTAACCAACAAGGCCGACGATTCGCCGGTTACCGCCGCCGACCTAGCCGCGCACCGGGTAATCGCCGATGGt                                                          <  2:5851360/149‑1 (MQ=255)
                                                                                         tGATGCTTGAAGTGGTCTAGCTGGCGCTGACGGCAGGCGATGCGATCCTGCCATTCTTGCGCGCCGATGTCGCGGTAACCAACAAGGCCGACGATTCGCCGGTTACCGCCGCCGACCTTGACGCGCACCGGGTAATCGCCGATGGTctg                                                       <  1:2315580/149‑1 (MQ=255)
                                                                                              cATGAAGTGGTCAAGCTGGCGCTGACGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTCGCGGTAACCAACAAGGCCGACGATTCGCCGGTTACCGCCGCCGACCTAGCCGCGCACCGGGTAATCGCCGATGGTCTgctggc                                                  <  2:6878123/149‑1 (MQ=255)
                                                                                               aTGAAGTGGTCAAGCTGGCGCTGACGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTCGCGGTAACCAACAAGGCCGACGATTCGCCGGTTACCGCCGCCGACCTAGCCGCGCACCGGGTAATCGCCGATGGTCTgctggcg                                                 >  1:765456/1‑149 (MQ=255)
                                                                                                tGAAGTGGTCAAGCTGGCGCTGACGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTCGCGGTAACCAACAAGGCCGACGATTCGCCGGTTACCGCCGCCGACCTAGCCGCGCACCGGGTAATCGCCGATGGTCTgctggcgc                                                <  2:5658160/149‑1 (MQ=255)
                                                                                                tGAAGTGGTCAAGCTGGCGCTGACGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTCGCGGTAACCAACAAGGCCGACGATTCGCCGGTTACCGCCGCCGACCTAGCCGCGCACCGGGTAATCGCCGATGGTCTgctggcgc                                                >  1:2476681/1‑149 (MQ=255)
                                                                                                tGAAGTGGTCAAGCTGGCGCTGACGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTCGCGGTAACCAACAAGGCCGACGATTCGCCGGTTACCGCCGCCGACCTAGCCGCGCACCGGGTAATCGCCGATGGTCTgctggcgc                                                >  2:1452355/1‑149 (MQ=255)
                                                                                                     tGGTCAAGCTGGCGCTGACGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTCGCGGTAACCAACAAGGCCGACGATTCGCCGGTTACCGCCGCCGACCTAGCCGCGCACCGGGTAATCGCCGATGGTCTGctggcgctggcg                                           >  1:1251886/1‑149 (MQ=255)
                                                                                                      ggTCAAGCTGGCGCTGACGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTCGCGGTAACCAACAAGGCCGACGATTCGCCGGTTACCGCCGCCGACCTAGCCGCGCACCGGGTAATCGCCGATGGTCTGCtggcgctggcgc                                          <  2:6096166/149‑1 (MQ=255)
                                                                                                        tCAAGCTGGCGCTGACGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTCGCGGTAACCAACAAGGCCGACGATTCGCCGGTTACCGCCGCCGACCTAGCCGCGCACCGGGTAATCGCCGATGGTCTGCTGGCGCTGGcgccg                                        <  2:2017642/149‑1 (MQ=255)
                                                                                                         cAAGCTGGCGCTGACGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTCGCGGTAACCAACAAGGCCGACGATTCGCCGGTTACCGCCGCCGACCTAGCCGCGCACCGGGTAATCGCCGATGGTCTGCTGGCGCTGGcgccgc                                       >  2:3337881/1‑149 (MQ=255)
                                                                                                         cAAGCTGGCGCTGACGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTCGCGGTAACCAACAAGGCCGACGATTCGCCGGTTACCGCCGCCGACCTAGCCGCGCACCGGGTAATCGCCGATGGTCTGCTGGCGCTGGcgccgc                                       >  1:1882885/1‑149 (MQ=255)
                                                                                                         cAAGCTGGCGCTGACGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTCGCGGTAACCAACAAGGCCGACGATTCGCCGGTTACCGCCGCCGACCTAGCCGCGCACCGGGTAATCGCCGATGGTCTGCTGGCGCTGGcgccgc                                       <  1:4108361/149‑1 (MQ=255)
                                                                                                                gcgcTGACGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTCGCGGTAACCAACAAGGCCGACGATTCGCCGGTTACCGCCGCCGACCTAGCCGCGCACCGGGTAATCGCCGATGGTCTGCTGGCGCTGGCGCCGCAGATTcc                                <  2:2137523/149‑1 (MQ=255)
                                                                                                                 cgcTGACGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTCGCGGTAACCAACAAGGCCGACGATTCGCCGGTTACCGCCGCCGACCTAGCCGCGCACCGGGTAATCGCCGATGGTCTGCTGGCGCTGGCGCCGCAGATTCCg                               >  1:7373308/1‑149 (MQ=255)
                                                                                                                   cTGACGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTCGCGGTAACCAACAAGGCCGACGATTCGCCGGTTACCGCCGCCGACCTAGCCGCGCACCGGGTAATCGCCGATGGTCTGCTGGCGCTGGCGCCGCAGATTCCGGt                             <  1:4584771/149‑1 (MQ=255)
                                                                                                                   cTGACGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTCGCGGTAACCAACAAGGCCGACGATTCGCCGGTTACCGCCGCCGACCTAGCCGCGCACCGGGTAATCGCCGATGGTCTGCTGGCGCTGGCGCCGCAGATTCCGGt                             <  2:3836035/149‑1 (MQ=255)
                                                                                                                     gACGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTCGCGGTAACCAACAAGGCCGACGATTCGCCGGTTACCGCCGCCGACCTAGCCGCGCACCGGGTAATCGCCGATGGTCTGCTGGCGCTGGCGCCGCAGATTCCGGTGc                           >  2:7096475/1‑149 (MQ=255)
                                                                                                                     gACGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTCGCGGTAACCAACAAGGCCGACGATTCGCCGGTTACCGCCGCCGACCTAGCCGCGCACCGGGTAATCGCCGATGGTCTGCTGGCGCTGGCGCCGCAGATTCCGGTGc                           >  2:5985930/1‑149 (MQ=255)
                                                                                                                       cGGCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTCGCGGTAACCAACAAGGCCGACGATTCGCCGGTTACCGCCGCCGACCTAGCCGCGCACCGGGTAATCGCCGATGGTCTGCTGGCGCTGGCGCCGCAGATTCCGGTGCTg                         >  1:3794386/1‑149 (MQ=255)
                                                                                                                        ggCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTCGCGGTAACCAACAAGGCCGACGATTCGCCGGTTACCGCCGCCGACCTAGCCGCGCACCGGGTAATCGCCGATGGTCTGCTGGCGCTGGCGCCGCAGAGTCCGGTGCTGt                        <  2:2724012/149‑1 (MQ=255)
                                                                                                                         gCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTCGCGGTAACCAACAAGGCCGACGATTCGCCGGTTACCGCCGCCGACCTAGCCGCGCACCGGGTAATCGCCGATGGTCTGCTGGCGCTGGCGCCGCAGATTCCGGTGCTGTc                       >  1:791784/1‑149 (MQ=255)
                                                                                                                         gCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTCGCGGTAACCAACAAGGCCGACGATTCGCCGGTTACCGCCGCCGACCTAGCCGCGCACCGGGTAATCGCCGATGGTCTGCTGGCGCTGGCGCCGCAGATTCCGGTGCTGTc                       >  2:6142390/1‑149 (MQ=255)
                                                                                                                         gCAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTCGCGGTAACCAACAAGGCCGACGATTCGCCGGTTACCGCCGCCGACCTAGCCGCGCACCGGGTAATCGCCGATGGTCTGCTGGCGCTGGCGCCACAGATTCCGGTGCTGTc                       >  2:1836443/1‑149 (MQ=255)
                                                                                                                          cAGGCGAGGCGATCCTGCCATTCTTGCGCGCCGATGTCGCGGTAACCAACAAGGCCGACGATTCGCCGGTTACCGCCGCCGACCTAGCCGCGCACCGGGTAATCGCCGATGGTCTGCTGGCGCTGGCGCCGCAGATTCCGGTGCTGTcc                      >  2:3427170/1‑149 (MQ=255)
                                                                                                                           aggcgaggcgATCCTGCCATTCTTGCGCGCCGATGTCGCGGTAACCAACAAGGCCGACGATTCGCCGGTTACCGCCGCCGACCTAGCCGCGCACCGGGTAATCGCCGATGGTCTGCTGGCGCTGGCGCCGCAGATTCCGGTGCTGTCCg                     >  1:6745748/1‑149 (MQ=255)
                                                                                                                            ggcgaggcgaTCCTGCCATTCTTGCGCGCCGATGTCGCGGTAACCAACAAGGCCGACGATTCGCCGGTTACCGCCGCCGACCTAGCCGCGCACCGGGTAATCGCCGATGGTCTGCTGGCGCTGGCGCCGCAGATTCCGGTGCTGTCCGa                    >  1:348051/1‑149 (MQ=255)
                                                                                                                                aggcgaTCCTGCCATTCTTGCGCGCCGATGTCGCGGTAACCAACAAGGCCGACGATTCGCCGGTTACCGCCGCCGACCTAGCCGCGCACCGGGTAATCGCCGATGGTCTGCTGGCGCTGGCGACGCAGATTCCGGTGCTGTCCGAagag                >  2:6655728/1‑149 (MQ=255)
                                                                                                                                 ggcgaTCCTGCCATTCTTGCGCGCCGATGTCGCGGTAACCAACAAGGCCGACGATTCGCCGGTTACCGCCGCCGACCTAGCCGCGCACCGGGTAATCGCCGATGGTCTGCTGGCGCTGGCGCCGCAGATTCCGGTGCTGTCCGAAGAgg               <  1:5801530/149‑1 (MQ=255)
                                                                                                                                 ggcgaTCCTGCCATTCTTGCGCGCCGATGTCGCGGTAACCAACAAGGCCGACGATTCGCCGGTTACCGCCGCCGACCTAGCCGCGCACCGGGTAATCGCCGATGGTCTGCTGGCGCTGGCGCCGCAGATTCCGGTGCTGTCCGAAGAgg               <  1:3120376/149‑1 (MQ=255)
                                                                                                                                   cgaTCCTGCCATTCTTGCGCGCCGATGTCGCGGTAACCAACAAGGCCGACGATTCGCTGGTTACCGCCGCCGACCTAGCCGCGCACCGGGTAATCGCCGATGGTCTGCTGGCGCTGGCGCCGCAGATTCCGGTGCTGTCCGAAGAGGAc             <  2:439021/149‑1 (MQ=255)
                                                                                                                                    gaTCCTGCCATTCTTGCGCGCCGATGTCGCGGTAACCAACAAGGCCGACGATTCGCCGGTTACCGCCGCCGACCTAGCCGCGCACCGGGTAATCGCCGATGGTCTGCTGGCGCTGGCGCCGCAGATTCCGGTGCTGTCCGAAGAGGACt            <  2:6498403/149‑1 (MQ=255)
                                                                                                                                      tCCTGCCATTCTTGCGCGCCGATGTCGCGGTAACCAACAAGGCCGACGATTCGCCGGTTACCGCCGCCGACCTAGCCGCGCACCGGGTAATCGCCGATGGTCTGCTGGCGCTGGCGCCGCAGATTCCGGTGCTGTCCGAAGAGGACTGc          >  1:6401305/1‑149 (MQ=255)
                                                                                                                                         tGCCATTCTTGCGCGCCGATGTCGCGGTAACCAACAAGGCCGACGATTCGCCGGTTACCGCCGCCGACCTAGCCGCGCACCGGGTAATCGCCGATGGTCTGCTGGCGCTGGCGCCGCAGATTCCGGTGCTGTCCGAAGAGGACTGCAat       >  2:2412787/1‑149 (MQ=255)
                                                                                                                                           ccATTCTTGCGCGCCGATGTCGCGGTAACCAACAAGGCCGACGATTCGCCGGTTACCGCCGCCGACCTAGCCGCGCACCGGGTAATCGCCGATGGTCTGCTGGCGCTGGCGCCGCAGATTCCGGTGCTGTCCGAAGAGGACTGCAatat     <  2:5552073/149‑1 (MQ=255)
                                                                                                                                           ccATTCTTGCGCGCCGATGTCGCGGTAACCAACAAGGCCGACGATTCGCCGGTTACCGCCGCCGACCTAGCCGCGCACCGGGTAATCGCCGATGGTCCGCTGGCGCTGGCGCCGCAGATTCCGGTGCTGTCCGAAGAGGACTGCAatat     >  1:2549135/1‑149 (MQ=255)
                                                                                                                                              ttCTTGCGCGCCGATGTCGCGGTAACCAACAAGGCCGACGATTCGCCGGTTACCGCCGCCGACCTAGCCGCGCACCGGGTAATCGCCGATGGTCTGCTGGCGCTGGCGCCGCAGATTCCGGTGCTGTCCGAAGAGGACTGCAATATTgc  <  1:2911860/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
TCACCGAGGGCCGCGCCCTGGCGGCGCTGTACCTGGCCCGTGACCTGCTGATTCAGCGGGGGCTGCTGGAGCTATGAACGACCTGCAACTGATGCATGAAGTGGTCAAGCTGGCGCTGACGGCAGGCGAGGCGATCCTGCCATTCTGGCGCGCCGATGTCGCGGTAACCAACAAGGCCGACGATTCGCCGGTTACCGCCGCCGACCTAGCCGCGCACCGGGTAATCGCCGATGGTCTGCTGGCGCTGGCGCCGCAGATTCCGGTGCTGTCCGAAGAGGACTGCAATATTGC  >  NC_002947/315097‑315387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑