Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 3,824,314 C→G A279P (GCG→CCG)  kguT ← 2‑ketogluconate transporter, putative

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,824,3140CG97.1% 124.5 / NA 35A279P (GCG→CCG) kguT2‑ketogluconate transporter, putative
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base G (16/18);  total (17/18)
Fisher's exact test for biased strand distribution p-value = 4.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCTCGCTGCCCAAGGCGTAGGAACCATAGAACGCCAACGCGGCAATCAGCAGCGGCGGCCAGACGAAGCGCTTGCGCTTTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGCCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAGCCACAGCACGAAGCCATACACGCCGATGCTCCAGCAGAAGTACTGCAGCGCCAGGA  >  NC_002947/3824184‑3824445
                                                                                                                                  |                                                                                                                                   
tGCTCGCTGCCCAAGGCGTAGGAACCATAGAACGCCATCGCGGCAATCAGCAGCGGCGGCCAGACGAAGCGCTTGCGCTTTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGt                                                                                                                                <  2:396211/136‑1 (MQ=255)
 gCTCGCTGCCCAAGGCGTAGGAACCATAGAACGCCAACGCGGCAATCAGCAGCGGCGGCCAGACGAAGCGCTTGCGCTTTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTa                                                                                                                               >  2:197401/1‑136 (MQ=255)
     gCTGCCCAAGGCGTAGGAACCATAGAACGCCAACGCGGCAATCAGCAGCGGCGGCCAGACGAAGCGCTTGCGCTTTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGc                                                                                                                           >  2:148793/1‑136 (MQ=255)
          ccAAGGCGTAGGAACCATAGAACGCCAACGCGGCAATCAGCAGCGGCGGCCAGACGAAGCGCTTGCGCTTTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCgc                                                                                                                      >  2:434724/1‑135 (MQ=255)
          ccAAGGCGTAGGAACCATAGAACGCCAACGCGGCAATCAGCAGCGGCGGCCAGACGAAGCGCTTGCGCTTTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGa                                                                                                                      >  1:197339/1‑136 (MQ=255)
          ccAAGGCGTAGGAACCATAGAACGCCAACGCGGCAATCAGCAGCGGCGGCCAGACGAAGCGCTTGCGCTTTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGa                                                                                                                      >  1:634944/1‑136 (MQ=255)
            aaGGCGTAGGAACCATAGAACGCCAACGCGGCAATCAGCAGCGGCGGCCAGACGAAGCGCTTGCGCTTTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGAgg                                                                                                                    <  2:645472/136‑1 (MQ=255)
                 gTAGGAACCATAGAACGCCAACGCGGCAATCAGCAGCGGCGGCCAGACGAAGCGCTTGCGCTTTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGAGgccagc                                                                                                               <  2:397991/136‑1 (MQ=255)
                    ggAACCATAGAACGCCAACGCGGCAATCAGCAGCGGCGGCCAGACGAAGCGCTTGCGCTTTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGccagccag                                                                                                            <  2:792896/136‑1 (MQ=255)
                    ggAACCATAGAACGCCAACGCGGCAATCAGCAGCGGCGGCCAGACGAAGCGCTTGCGCTTTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGccagccag                                                                                                            <  2:396115/136‑1 (MQ=255)
                    ggAACCATAGAACGCCAACGCGGCAATCAGCAGCGGCGGCCAGACGAAGCGCTTGCGCTTTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGccagccag                                                                                                            <  2:451053/136‑1 (MQ=255)
                      aaCCATAGAACGCCAACGCGGCAATCAGCAGCGGCGGCCAGACGAAGCGCTTGCGCTTTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCagccagcc                                                                                                          >  1:70363/1‑136 (MQ=255)
                         cATAGAACGCCAACGCGGCAATCAGCAGCGGCGGCCAGACGAAGCGCTTGCGCTTTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGc                                                                                                       <  1:131789/136‑1 (MQ=255)
                           tAGAACGCCAACGCGGCAATCAGCAGCGGCGGCCAGACGAAGCGCTTGCGCTTTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCt                                                                                                     >  1:310681/1‑136 (MQ=255)
                           tAGAACGCCAACGCGGCAATCAGCAGCGGCGGCCAGACGAAGCGCTTGCGCTTTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCt                                                                                                     <  2:84282/136‑1 (MQ=255)
                            aGAACGCCAACGCGGCAATCAGCAGCGGCGGCCAGACGAAGCGCTTGCGCTTTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTg                                                                                                    >  2:208479/1‑136 (MQ=255)
                                  ccAACGCGGCAATCAGCAGCGGCGGCCAGACGAAGCGCTTGCGCTTTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGat                                                                                              <  2:305559/136‑1 (MQ=255)
                                   cAACGAGGCAATCAGCAGCGGCGGCCAGACGAAGCGCTTGCTCTTTAGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGCCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGata                                                                                             >  1:799222/1‑136 (MQ=255)
                                        cgGCAATCAGCAGCGGCGGCCAGACGAAGCGCTTGCGCTTTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGAc                                                                                        >  2:206140/1‑136 (MQ=255)
                                          gCAATCAGCAGCGGCGGCCAGACGAAGCGCTTGCGCTTTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGt                                                                                      <  2:310681/136‑1 (MQ=255)
                                             aTCAGCAGCGGCGGCCAGACGAAGCGCTTGCGCTTTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTcg                                                                                   >  2:145534/1‑136 (MQ=255)
                                               tagcagcGGCGGCCAGACGAAGCGCTTGCGCTTTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTcgcc                                                                                 <  2:96927/135‑1 (MQ=255)
                                                                      gcttgcgcttTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAg                                                          <  1:148793/136‑1 (MQ=255)
                                                                            gcttTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAGCCACAg                                                    >  1:825868/1‑136 (MQ=255)
                                                                                       gggTCGTACGCCCAGGACACGCCCACAATTGCCAGCACCGCCGGCAGGTAAGGCACCTAGGCCAGCCCGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGAGGGCAGCCACAGCACGACGCCAt                                         <  1:197401/135‑1 (MQ=255)
                                                                                             gACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAGCCACAGCACGAAGCCATACACGc                                   <  1:206140/136‑1 (MQ=255)
                                                                                                   cAGGACACGCCCACCATTGCCAGACCCGCGGGCATGTACGGCACCTTGCCCTGCCTGCCGGCCTGCACGATATCCACGTTCCCCGCGTGCTTGAGCATCGACGGCACCCACAGCACTACCCCAAACACGCCGATGc                             <  2:825868/136‑1 (MQ=255)
                                                                                                     gcacacGCCCACCATTGCCACCACCGCCGGCAGGTAAGGCACGGAGGCCAGCCAGCGGGCCTGCACTATTTCGACGTGCGCCGCGTGGTTGAGGAGCGACGGCAGCCACAGCACGAAGCCATACACGCCGATGCTc                           <  2:684558/134‑1 (MQ=255)
                                                                                                             ccaccaTTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAGCCACAGCACGAAGCCATACACGCCGATGCTCCAGCAGaa                   >  2:213683/1‑136 (MQ=255)
                                                                                                              caccaTTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAGCCACAGCACGAAGCCATACACGCCGATGCTCCAGCAGAAg                  >  1:684558/1‑136 (MQ=255)
                                                                                                               accaTTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAGCCACAGCACGAAGCCATACACGCCGATGCTCCAGCAGAAGt                 >  1:720337/1‑136 (MQ=255)
                                                                                                               accaTTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAGCCACAGCACGAAGCCATACACGCCGATGCTCCAGCAGAAGt                 >  2:539844/1‑136 (MQ=255)
                                                                                                                       cAGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAGCCACAGCACTAAGCCATACACGCCGATGCTCCAGCAGAAGTACTGCAgc         <  1:208479/136‑1 (MQ=255)
                                                                                                                       cAGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAGCCACAGCACGAAGCCATACACGCCGATGCTCCAGCAGAAGTACTGCAgc         <  1:657061/136‑1 (MQ=255)
                                                                                                                        aGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAGCCACAGCACGAAGCCATACACGCCGATGCTCCAGCAGAAGTACTGCAgcg        >  1:666926/1‑136 (MQ=255)
                                                                                                                             cgccggCAGGTAAGGCACCGAGGCCACCCAGCCGGCCTGCACGATATCGACGTGCGCCGCCTGCTTGAGGATCGACGGCAGCCACACCACGACGCCATACACGCCGATGCTCCAGCAGAAGTACTGCAGCGCCAgg   <  2:70363/136‑1 (MQ=255)
                                                                                                                              gccggcAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAGCCACAGCACGAAGCCATACACGCCGATGCTCCAGCAGAAGTACTGCAGCGCCAGga  >  1:701918/1‑136 (MQ=255)
                                                                                                                                  |                                                                                                                                   
TGCTCGCTGCCCAAGGCGTAGGAACCATAGAACGCCAACGCGGCAATCAGCAGCGGCGGCCAGACGAAGCGCTTGCGCTTTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGCCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAGCCACAGCACGAAGCCATACACGCCGATGCTCCAGCAGAAGTACTGCAGCGCCAGGA  >  NC_002947/3824184‑3824445

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑