Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	5,060,944	(C)6→7	coding (279/1128 nt)	ooxB ←	opine oxidase subunit B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,060,938	1	.	C	85.7%	14.3 / ‑2.2	7	coding (285/1128 nt)	ooxB	opine oxidase subunit B
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base C (4/2);  total (4/3)
Fisher's exact test for biased strand distribution p-value = 4.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.51e-01

TTGGAAATCGACGCAGCAGTTCAGCTCGATCTAGCATGTGGGCTGAAGGAGCGAGTTCTGGTGTTTGCCTGTTCCAGGCATCCATCGCCTTCGCCCGAGCATCGAACTCATCGTCACTGAGGCAGAAGTTCAAG‑CCCCCCTTCTGCTCGTAGTCCAGCGAGATGCCGCTTTCTTCCTCAAGCTGCGAAG  >  NC_002947/5060805‑5060993                                                                                                                                       |                                                        ttgGAAATCGACGCAGCAGTTCAGCTCGATCTAGCATGTGGGCTGAAGGAGCGAGTTCTGGTGTTTGCCTGTTCCAGGCATCCATCGCCTTCGCCCGAGCATCGAACTCATCGTCACTGAGGCAGAAGTTCAAG‑cc                                                       <  1:101041/136‑1 (MQ=255)            cGCAGCAGTTCAGCTCGATCTAGCATGTGGGCTGAAGGAGCGAGTTCTGGTGTTTGCCTGTTCCAGGCATCCATCGCCTTCGCCCGAGCATCGAACTCATCGTCACTGAGGCAGAAGTTCAAGCCCCCCCTTCTGc                                             <  1:63842/136‑1 (MQ=255)                                 aGCATGTGGGCTGAAGGAGCGAGTTCTGGTGTTTGCCTATTCCAGGCATCCATCGCCTTCGCCCGAGCATCGAACTCATCGTCACTGAGGCAGAAGTTCAAG‑CCCCCCTTCTGCTCGTAGTCCAGCGAGATGCCGc                       <  1:1336410/136‑1 (MQ=255)                                         ggCTGAAGGAGCGAGTTCTGGTGTTTGCCTGTTCCAGGCATCCATCGCCTTCGCCCGAGCATCGAACTCATCGTCACTGAGGCAGAAGTTCAAGCCCCCCCTTCTGCTCGTAGTCCAGCGAGATGCCGCTttcttc                >  1:402916/1‑136 (MQ=255)                                          gCTGAAGGAGCGAGTTCTGGTGTTTGCCTGTTCCAGGCATCCATCGCCTTCGCCCGAGCATCGAACTCATCGTCACTGAGGCAGAAGTTCAAGCCCCCCCTTCTGCTCGTAGTCCAGCGAGATGCCGCTTTCTTcc               <  2:618889/136‑1 (MQ=255)                                           cTGAAGGAGCGAGTTCTGGTGTTTGCCTGTTCCAGGCATCCATCGCCTTCGCCCGAGCATCGAACTCATCGTCACTGAGGCAGAAGTTCAAGCCCCCCCTTCTGCTCGTAGTCCAGCGAGATGCCGCTTTCTTccg              >  1:48155/1‑135 (MQ=255)                                           cTGAAGGAGCGAGTTCTGGTGTTTGCCTGTTCCAGGCATCCATCGCCTTCGCCCGAGCATCGAACTCATCGTCACTGAGGCAGAAGTTCAAGCCCCCCCTTCTGCTCGTAGTCCAGCGAGATGCCGCTTTCTTCCt              >  1:950598/1‑136 (MQ=255)                                                       gTTCTGGTGTTTGCCTGTTCCAGGCATCCATCGCCTTCGCCCGAGCATCGAACTCATCGTCACTGAGGCAGAAGTTCAAGCCCCCCCTTCTGCTCGTAGTCCAGCGAGATGCCGCTTTCTTCCTCAAGCTgcgaag  >  1:53490/1‑136 (MQ=255)                                                                                                                                       |                                                        TTGGAAATCGACGCAGCAGTTCAGCTCGATCTAGCATGTGGGCTGAAGGAGCGAGTTCTGGTGTTTGCCTGTTCCAGGCATCCATCGCCTTCGCCCGAGCATCGAACTCATCGTCACTGAGGCAGAAGTTCAAG‑CCCCCCTTCTGCTCGTAGTCCAGCGAGATGCCGCTTTCTTCCTCAAGCTGCGAAG  >  NC_002947/5060805‑5060993

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑