Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 1,261,817 (G)6→7 coding (824/1116 nt) PP_1104 ← AotO‑related protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,261,8111.G98.2% 199.4 / ‑3.1 54coding (830/1116 nt)PP_1104AotO‑related protein
Reads supporting (aligned to +/- strand):  ref base . (1/0);  new base G (22/31);  total (23/31)
Fisher's exact test for biased strand distribution p-value = 4.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01

CGATGACTTCGGCGATCAGTTGGCCGGCCTCCAGGTGCTGGCCCGGTTTGGCGTGGTAAACCAGCAACCCGCCCAGTGGCGTTGCCACAGGTTCCACCGCAGCCAACGGGGTGGCGTGGCGTAACAGCGCGGGCA‑GGGGGG‑‑CGCTGGTGCCTTCGATGACACCTGCGTGGGTCAGGAAGTCGAGGATCGCCTGGCAGTCCTTGCTGGCCAGATCATGGCTGACATCCGCCTGGCCACGCAGCTCCACGGTGACCGAG  >  NC_002947/1261677‑1261933
                                                                                                                                       |                                                                                                                            
cGATGACTTCGGCGATCAGTTGGCCGGCCTCCAGGTGCTGGCCCGGTTTGGCGTGGTAAACCAGCAACCCGCCCAGTGGCGTTGCCACAGGTTCCACCGCAGCCAACGGGGTGGCGTGGCGTAACAGCGCGGGCA‑g                                                                                                                             >  2:616403/1‑136 (MQ=255)
    gACTTCGGCGATCAGTTGGCCGGCCTCCAGGTGCTGGCCCGGTTTGGCGTGGTAAACCAGCAACCCGCCCAGTGGCGTTGCCACAGGTTCCACCGCAGCCAACGGGGTGGCGTGGCGTAACAGCGCGGGCA‑ggggg                                                                                                                         >  1:120118/1‑136 (MQ=255)
            cGATCAGTTGGCCGGCCTCCAGGTGCTGGCCCGGTTTGGCGTGGTAAACCAGCAACCCGCCCAGTGGCGTTGCCACAGGTTCCACCGCAGCCAACGGGGTGGCGTGGCGTAACAGCGCGGGCAGGGGGGG‑‑CGCTgg                                                                                                                >  1:1041408/1‑136 (MQ=255)
            cGATCAGTTGGCCGGCCTCCAGGTGCTGGCCCGGTTTGGCGTGGTAAACCAGCAACCCGCCCAGTGGCGTTGCCACAGGTTCCACCGCAGCCAACGGGGTGGCGTGGCGTAACAGCGCGGGCAGGGGGGG‑‑CGCTgg                                                                                                                >  1:754904/1‑136 (MQ=255)
              aTCAGTTGGCCGGCCTCCAGGTGCTGGCCCGGTTTGGCGTGGTAAACCAGCAACCCGCCCAGTGGCGTTGCCACAGGTTCCACCGCAGCCAACGGGGTGGCGTGGCGTAACAGCGCGGGCAGGGGGGG‑‑CGCTGGTg                                                                                                              <  1:1223226/136‑1 (MQ=255)
              aTCAGTTGGCCGGCCTCCAGGTGCTGGCCCGGTTTGGCGTGGTAAACCAGCAACCCGCCCAGTGGCGTTGCCACAGGTTCCACCGCAGCCAACGGGGTGGCGTGGCGTAACAGCGCGGGCAGGGGGGG‑‑CGCTGGTg                                                                                                              <  2:512146/136‑1 (MQ=255)
              aTCAGTTGGCCGGCCTCCAGGTGCTGGCCCGGTTTGGCGTGGTAAACCAGCAACCCGCCCAGTGGCGTTGCCACAGGTTCCACCGCAGCCAACGGGGTGGCGTGGCGTAACAGCGCGGGCAGGGGGGG‑‑CGCTGGTg                                                                                                              <  2:256979/136‑1 (MQ=255)
              aTCAGTTGGCCGGCCTCCAGGTGCTGGCCCGGTTTGGCGTGGTAAACCAGCAACCCGCCCAGTGGCGTTGCCACAGGTTCCACCGCAGCCAACGGGGTGGCGTGGCGTAACAGCGCGGGCAGGGGGGG‑‑CGCTGGTg                                                                                                              <  2:246281/136‑1 (MQ=255)
                   ttGGCCGGCCTCCAGGTGCTGGCCCGGTTTGGCGTGGTAAACCAGCAACCCGCCCAGTGGCGTTGCCACAGGTTCCACCGCAGCCAACGGGGTGGCGTGGCGTAACAGCGCGGGCAGGGGGGG‑‑CGCTGGTGCCTTc                                                                                                         <  2:754904/136‑1 (MQ=255)
                   ttGGCCGGCCTCCAGGTGCTGGCCCGGTTTGGCGTGGTAAACCAGCAACCCGCCCAGTGGCGTTGCCACAGGTTCCACCGCAGCCAACGGGGTGGCGTGGCGTAACAGCGCGGGCAGGGGGGG‑‑CGCTGGTGCCTTc                                                                                                         <  1:604636/136‑1 (MQ=255)
                             tCCAGGTTCTGGCCCGGTTTGGCGTGGTAAACCAGCAACCCGCCCAGTGGCGTTGCCACAGGTTCCACCGCAGCCAACGGGGTGGCGTGGCGTAACAGCGCTGGCAGGGGGGG‑‑CGCTGGTGCCTTCGATGACACCt                                                                                               <  2:1129900/136‑1 (MQ=255)
                             tCCAGGTGCTGGCCCGGTTTGGCGTGGTAAACCAGCAACCCGCCCAGTGGCGTTGCCACAGGTTCCACCGCAGCCAACGGGGTGGCGTGGCGTAACAGCGCGGGCAGGGGGGG‑‑CGCTGGTGCCTTCGATGACACCt                                                                                               <  1:268060/136‑1 (MQ=255)
                             cccAGGGGCTGGCCCGGTTTGGCGTGGAAAACCAGCAACCCGCCCAGTGGCGTTGCCACAGGTTCCACCGCAGCCAACGGGGTGGCGTGGCGTAACAGCGCGGGCAGGGGGGG‑‑CGCTGGTGCCTTCGATGACACCt                                                                                               <  2:907696/135‑1 (MQ=255)
                               cAGGTGCTGGCCCGGTTTGGCGTGGTAAACCAGCAACCCGCCCAGTGGCGTTGCCACAGGTTCCACCGCAGCCAACGGGGTGGCGTGGCGTAACAGCGCGGGCAGGGGGGG‑‑CGCTGGTGCCTTCGATGACACCTGc                                                                                             <  2:9258/136‑1 (MQ=255)
                                      tGGCCCGGTTTGGCGTGGTAAACCAGCAACCCGCCCAGTGGCGTTGCCACAGGTTCCACCGCAGCCAACGGGGTGGCGTGGCGTAACAGCGCGGGCAGGGGGGG‑‑CGCTGGTGCCTTCGATGACACCTGCGTGGGTc                                                                                      <  1:569477/136‑1 (MQ=255)
                                          ccGGTTTGGCGTGGTAAACCAGCAACCCGCCCAGTGGCGTTGCCACAGGTTCCACCGCAGCCAACGGGGTGGCGTGGCGTAACAGCGCGGGCAGGGGGGG‑‑CGCTGGTGCCTTCGATGACACCTGCGTGGGTCAGGa                                                                                  <  2:1252076/136‑1 (MQ=255)
                                           cGGTTTGGCGTGGTAAACCAGCAACCCGCCCAGTGGCGTTGCCACAGGTTCCACCGCAGCCAACGGGGTGGCGTGGCGTAACAGCGCGGGCAGGGGGGG‑‑CGCTGGTGCCTTCGATGACACCTGCGTGGGTCAGGaa                                                                                 >  2:1234755/1‑136 (MQ=255)
                                              tttGGCGTGGTAAACCAGCAACCCGCCCAGTGGCGTTGCCACAGGTTCCACCGCAGCCAACGGGGTGGCGTGGCGTAACAGCGCGGGCAGGGGGGG‑‑CGCTGGTGCCTTCGATGACACCTGCGTGGGTCAGGAAGTc                                                                              <  1:897459/136‑1 (MQ=255)
                                                ttgCGGGGTTAACCAGCAACCCGCCCAGTTGCGTTGCCACATGTTCCACCGGAGCCAACGGGGTTGCGTGGGGTTACAGCGCGGGCAGGGGGGG‑‑CGCTGGTGCCTTCGATGACACCTGCGTGGGTCAGGAAGTCGa                                                                            <  2:1201671/134‑1 (MQ=255)
                                                tGGCGTGGTAAACCAGCAACCCGCCCAGTGGCGTTGCCACAGGTTCCACCGCAGCCAACGGGGTGGCGTGGCGTAACAGCGCGGGCAGGGGGGG‑‑CGCTGGTGCCTTCGATGACACCTGCGTGGGTCAGGAAGTCGa                                                                            >  2:153087/1‑136 (MQ=255)
                                                tGGCGTGGTAAACCAGCAACCCGCCCAGTGGCGTTGCCACAGGTTCCACCGCAGCCAACGGGGTGGCGTGGCGTAACAGCGCGGGCAGGGGGGG‑‑CGCTGGTGCCTTCGATGACACCTGCGTGGGTCAGGAAGTCGa                                                                            >  1:1016602/1‑136 (MQ=255)
                                                tGGCGTGGTAAACCAGCAACCCGCCCAGTGGCGTTGCCACAGGTTCCACCGCAGCCAACGGGGTGGCGTGGCGTAACAGCGCGGGCAGGGGGGG‑‑CGCTGGTGCCTTCGATGACACCTGCGTGGGTCAGGAAGTCGa                                                                            >  1:868501/1‑136 (MQ=255)
                                                tGGCGTGGTAAACCAGCAACCCGCCCAGTGGCGTTGCCACAGGTTCCACCGCAGCCAACGGGGTGGCGTGGCGTAACAGCGCGGGCAGGGGGGG‑‑CGCTGGTGCCTTCGATGACACCTGCGTGGGTCAGGAAGTCGa                                                                            <  1:692357/136‑1 (MQ=255)
                                                        tAAACCAGCAACCCGCCCAGTGGCGTTGCCACAGGTTCCACCGCAGCCAACGGGGTGGCGTGGCGTAACAGCGCGGGCAGGGGGGG‑‑CGCTGGTGCCTTCGATGACACCTGCGTGGGTCAGGAAGTCGAGGATCGcc                                                                    <  1:1205730/136‑1 (MQ=255)
                                                          aaCCAGCAACCCGCCCAGTGGCGTTGCCACAGGTTCCACCGCAGCCAACGGGGTGGCGTGGCGTAACAGCGCGGGCAGGGGGGG‑‑CGCTGGTGCCTTCGATGACACCTGCGTGGGTCAGGAAGTCGAGGATCGCCTg                                                                  >  1:93449/1‑136 (MQ=255)
                                                          aaCCAGCAACCCGCCCAGTGGCGTTGCCACAGGTTCCACCGCAGCCAACGGGGTGGCGTGGCGTAACAGCGCGGGCAGGGGGGG‑‑CGCTGGTGCCTTCGATGACACCTGCGTGGGTCAGGAAGTCGAGGATCGCCTg                                                                  >  1:692247/1‑136 (MQ=255)
                                                           aCCAGCAACCCGCCCAGTGGCGTTGCCACAGGTTCCACCGCAGCCAACGGGGTGGCGTGGTGTAACAGCGCGGGCAGGGGGGG‑‑CGCTGGTGCCTTCGATGACACCTGCGTGGGTCAGGAAGTCGAGGATCGCCTgg                                                                 >  1:61606/1‑136 (MQ=255)
                                                           aCCAGCAACCCGCCCAGTGGCGTTGCCACAGGTTCCACCGCAGCCAACGGGGTGGCGTGGCGTAACAGCGCGGGCAGGGGGGG‑‑CGCTGGTGCCTTCGATGACACCTGCGTGGGTCAGGAAGTCGAGGATCGCCTgg                                                                 >  2:1274486/1‑136 (MQ=255)
                                                                   cccGCCCAGTGGCGTTGCCACAGGTTCCACCGCAGCCAACGGGGTGGCGTGGCGTAACAGCGCGGGCAGGGGGGG‑‑CGCTGGTGCCTTCGATGACACCTGCGTGGGTCAGGAAGTCGAGGATCGCCTGGCAGTCCtt                                                         >  2:213820/1‑136 (MQ=255)
                                                                   cccGCCCAGTGGCGTTGCCACAGGTTCCACCGCAGCCAACGGGGTGGCGTGGCGTAACAGCGCGGGCAGGGGGGG‑‑CGCTGGTGCCTTCGATGACACCTGCGTGGGTCAGGAAGTCGAGGATCGCCTGGCAGTCCtt                                                         >  1:952760/1‑136 (MQ=255)
                                                                      gCCCAGTGGCGTTGCCACAGGTTCCACCGCAGCCAACGGGGTGGCGTGGCGTAACAGCGCGGGCAGGGGGGG‑‑CGCTGGTGCCTTCGATGACACCTGCGTGGGTCAGGAAGTCGAGGATCGCCTGGCAGTCCTTGCt                                                      <  1:213820/136‑1 (MQ=255)
                                                                           gTGGCGTTGCCACAGGTTCCACCGCAGCCAACGGGGTGGCGTGGCGTAACAGCGCGGGCAGGGGGGG‑‑CGCTGGTGCCTTCGATGACACCTGCGTGGGTCAGGAAGTCGAGGATCGCCTGGCAGTCCTTGCTGGCCa                                                 <  1:1065497/136‑1 (MQ=255)
                                                                           gTGGCGTTGCCACAGGTTCCACCGCAGCCAACGGGGTGGCGTGGCGTAACAGCGCGGGCAGGGGGGG‑‑CGCTGGTGCCTTCGATGACACCTGCGTGGGTCAGGAAGTCGAGGATCGCCTGGCAGTCCTTGCTGGCCa                                                 <  2:1016602/136‑1 (MQ=255)
                                                                               cGTTGCCACAGGTTCCACCGCAGCCAACGGGGTGGCGTGGCGTAACAGCGCGGGCAGGGGGGG‑‑CGCTGGTGCCTTCGATGACACCTGCGTGGGTCAGGAAGTCGAGGATCGCCTGGCAGTCCTTGCTGGCCAGATc                                             >  1:1060104/1‑136 (MQ=255)
                                                                               cGTTGCCACAGGTTCCACCGCAGCCAACGGGGTGGCGTGGCGTAACAGCGCGGGCAGGGGGGG‑‑CGCTGGTGCCTTCGATGACACCTGCGTGGGTCAGGAAGTCGAGGATCGCCTGGCAGTCCTTGCTGGCCAGATc                                             >  1:17489/1‑136 (MQ=255)
                                                                                    ccACAGGTTCCACCGCAGCCAACGGGGTGGCGTGGCGTAACAGCGCGGGCAGGGGGGG‑‑CGCTGGTGCCTTCGATGACACCTGCGTGGGTCAGGAAGTCGAGGATCGCCTGGCAGTCCTTGCTGGCCAGATCATGGc                                        >  2:900822/1‑136 (MQ=255)
                                                                                       caGGTTCCACCGCAGCCAACGGGGTGGCGTGGCGTAACAGCGCGGGCAGGGGGGG‑‑CGCTGGTGCCTTCGATGACACCTGCGTGGGTCAGGAAGTCGAGGATCGCCTGGCAGTCCTTGCTGGCCAGATCATGGCTGa                                     <  2:347644/136‑1 (MQ=255)
                                                                                           ttCCACCGCAGCCAACGGGGTGGCGTGGCGTAACAGCGCGGGCAGGGGGGG‑‑CGCTGGTGCCTTCGATGACACCTGCGTGGGTCAGGAAGTCGAGGATCGCCTGGCAGTCCTTGCTGGCCAGATCATGGCTGACATc                                 <  1:56378/136‑1 (MQ=255)
                                                                                             ccACCGCAGCCAACGGGGTGGCGTGGTGTAACAGCGCGGGCAGGGGGGG‑‑CGCTGGTGCCTTCGATGACACCTGCGTGGGTCAGGAAGTCGAGGATCGCCTGGCAGTCCTTGCTGGCCAGATCATGGCTGACATCCg                               <  2:61606/136‑1 (MQ=255)
                                                                                               cccGCAGCCAACGGGGTGGCGTGGCGTAACAGCGCGGGCAGGGGGGG‑‑CGCTGGTGCCTTCGATGACACCTGCGTGGGTCAGGAAGTCGAGGATCGCCTGGCAGTCCTTGCTGGCCAGATCATGGCTGACATCCGcc                             <  1:889895/135‑1 (MQ=255)
                                                                                               aCCGCAGCCAACGGGGTGGCGTGGCGTAACAGCGCGGGCAGGGGGGG‑‑CGCTGGTGCCTTCGATGACACCTGCGTGGGTCAGGAAGTCGAGGATCGCCTGGCAGTCCTTGCTGGCCAGATCATGGCTGACATCCGcc                             <  2:338820/136‑1 (MQ=255)
                                                                                                  ccAGCCAACGGGGTGGCGTGGCGTAACAGCGCGGGCAGGGGGGG‑‑CGCTGGTGCCTTCGATGACACCTGCGTGGGTCAGGAAGTCGAGGATCGCCTGGCAGTCCTTGCTGGCCAGATCATGGCTGACATCCGCCTgg                          <  1:300548/135‑1 (MQ=255)
                                                                                                       cAACGGGGTGGCGTGGCGTAACAGCGCGGGCAGGGGGGG‑‑CGCTGGTGCCTTCGATGACACCTGCGTGGGTCAGGAAGTCGAGGATCGCCTGGCAGTCCTTGCTGGCCAGATCATGGCTGACATCCGCCTGGCCACg                     >  2:879613/1‑136 (MQ=255)
                                                                                                        aaCGGGGTGGCGTGGCGTAACAGCGCGGGCAGGGGGGG‑‑CGCTGGTGCCTTCGATGACACCTGCGTGGGTCAGGAAGTCGAGGATCGCCTGGCAGTCCTTGCTGGCCAGATCATGGCTGACATCCGCCTGGCCACGc                    >  2:227005/1‑136 (MQ=255)
                                                                                                        aaCGGGGTGGCGTGGCGTAACAGCGCGGGCAGGGGGGG‑‑CGCTGGTGCCTTCGATGACACATGCGTCGGTCAGGAAGTCGAGGATCGCCTGGCAGTCCTTCCTGGCCAGATCATGGCTGACATCCGCCTGGCCACGc                    >  2:216186/1‑136 (MQ=255)
                                                                                                             ggTGGCGTGGCGTAACAGCGCGGGCA‑GGGGGGGCCGCTGGTGCCTTCGATGACACCTGCGTGGGTCAGGAAGTCGAGGATCGCCTGGCAGTCCTTGCTGGCCAGATCATGGCTGACATCCGCCTGGCCACGCAGcg                >  2:7011/1‑135 (MQ=255)
                                                                                                              gtggcgtggcGTAACAGCGCGGGCAGGGGGGG‑‑CGCTGGTGCCTTCGATGACACCTGCGTGGGTCAGGAAGTCGAGGATCGCCTGGCAGTCCTTGCTGGCCAGATCATGGCTGACATCCGCCTGGCCACGCAGCTcc              <  2:501234/136‑1 (MQ=255)
                                                                                                               gggcgtggcgtAACAGCGCGGGCAGGGGGGG‑‑CGCTGGTGCCTTCGATGACACCTGCGTGGGTCAGGAAGTCGAGGATCGCCTGGCAGTCCTTGCTGGCCAGATCATGGCTGACATCCGCCTGGCCACGCAGCTCCa             <  2:759561/135‑1 (MQ=255)
                                                                                                                 gcgtggcgtAACAGCGCGGGCAGGGGGGG‑‑CGCTGGTGCCTTCGATGACACCTGCGTGGGTCAGGAAGTCGAGGATCGCCTGGCAGTCCTTGCTGGCCAGATCATGGCTGACATCCGCCTGGCCACGCAGCTCCACg           >  1:212605/1‑136 (MQ=255)
                                                                                                                   gtggcgtAACAGCGCGGGCAGGGGGGG‑‑CGCTGGTGCCTTCGATGACACCTGCGTGGGTCAGGAAGTCGAGGATCGCCTGGCAGTCCTTGCTGGCCAGATCATGGCTGACATCCGCCTGGCCACGCAGCTCCACGGt         <  2:212605/136‑1 (MQ=255)
                                                                                                                   gtggcgtAACAGCGCGGGCAGGGGGGG‑‑CGCTGGTGCCTTCGATGACACCTGCGTGGGTCAGGAAGTCGAGGATCGCCTGGCAGTCCTTGCTGGCCAGATCATGGCTGACATCCGCCTGGCCACGCAGCTCCACGGt         >  1:64830/1‑136 (MQ=255)
                                                                                                                   gtggcgtAACAGCGCGGGCAGGGGGGG‑‑CGCTGGTGCCTTCGATGACACCTGCGTGGGTCAGGAACTCGAGGATCGCCTGGCAGTCCTTGCTGGCCAGATCATGGCTGACATCCGCCTGGCCACGCAGCTCCACGGt         <  1:761907/136‑1 (MQ=255)
                                                                                                                          aaCAGCGCGGGCAGGGGGGG‑‑CGCTGGTGCCTTCGATGACACCTGCGTGGGTCAGGAAGTCGAGGATCGCCTGGCAGTCCTTGCTGGCCAGATCATGGCTGACATCCGCCTGGCCACGCAGCTCCACGGTGACCGag  >  2:526792/1‑136 (MQ=255)
                                                                                                                          aaCAGCGCGGGCAGGGGGGG‑‑CGCTGGTGCCTTCGATGACACCTGCGTGGGTCAGGAAGTCGAGGATCGCCTGGCAGTCCTTGCTGGCCAGATCATGGCTGACATCCGCCTGGCCACGCAGCTCCACGGTGACCGag  >  2:1185754/1‑136 (MQ=255)
                                                                                                                          aaCAGCGCGGGCAGGGGGGG‑‑CGCTGGTGCCTTCGATGACACCTGCGTGGGTCAGGAAGTCGAGGATCGCCTGGCAGTCCTTGCTGGCCAGATCATGGCTGACATCCGCCTGGCCACGCAGCTCCACGGTGACCGag  <  1:931657/136‑1 (MQ=255)
                                                                                                                          aaCAGCGCGGGCAGGGGGGG‑‑CGCTGGTGCCTTCGATGACACCTGCGTGGGTCAGGAAGTCGAGGATCGCCTGGCAGTCCTTGCTGGCCAGATCATGGCTGACATCCGCCTGGCCACGCAGCTCCACGGTGACCGag  <  1:526792/136‑1 (MQ=255)
                                                                                                                                       |                                                                                                                            
CGATGACTTCGGCGATCAGTTGGCCGGCCTCCAGGTGCTGGCCCGGTTTGGCGTGGTAAACCAGCAACCCGCCCAGTGGCGTTGCCACAGGTTCCACCGCAGCCAACGGGGTGGCGTGGCGTAACAGCGCGGGCA‑GGGGGG‑‑CGCTGGTGCCTTCGATGACACCTGCGTGGGTCAGGAAGTCGAGGATCGCCTGGCAGTCCTTGCTGGCCAGATCATGGCTGACATCCGCCTGGCCACGCAGCTCCACGGTGACCGAG  >  NC_002947/1261677‑1261933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑