| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,931,807 | 0 | A | C | 52.4% | 2.8 / 17.7 | 21 | W423G (TGG→GGG) | PP_5603 | FAD dependent oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base A (8/2); new base C (11/0); total (19/2) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.14e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.62e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATAGTGGTACGCAGCTGGGTGGGCGGGATACCGGGACTTACCTGCAATTGATTGTCGGGACCTTTTTCTGATCCGACATCCTGGGGCTGCGAAGCAGCCCCGGCAATCTCAAACCAACCCCTGCTCCCCCAGCCACCGCCGCACGCTCCTCTGCTGTTCACCCGCAAGCCCCGACAATACCGCCTCAACAGGCCGCCCACTGCGTATTTGTCGCACCACCGGCGCCAGTTCCACCCCCTGCAAATGCCAAACCCCCAGCGGCTGGTCCGC > NC_002947/3931673‑3931942 | aTAGTGGTACGCAGCTGGGTGGGCGGGATACCGGGACTTACCTGCAATTGATTGTCGGGACCTTTTTCTGATCCGACATCCTGGGGCTGCGAAGCAGCCCCGGCAATCTCAAACCCACCCCTGCTCCCCCCGCCAc > 1:630764/1‑136 (MQ=255) cGCAGCTGGGTGGGCGGGATACCGGGACTTACCTGCAATTGATTGTCGGGACCTTTTTCTGATCCGACATCCTGGGGCTGCGAAGCAGCCCCGGCAATCTCAAACCACCCCCTGCGGCCCCCGCCCCCGCCGCACg > 2:701382/1‑136 (MQ=255) cGCAGCTGGGTGGGCGGGATACCGGGACTTACCTGCAATTGATTGTCGGGACCTTTTTCTGATCCGACATCCTGGGGCTGCGAAGCAGCCCCGGCAATCTCAAACCAACCCCTGCTCCCCCCGCCCCCGCCGCCCg > 2:315216/1‑136 (MQ=255) cGCAGCTGGGTGGGCGGGATACCGGGACTTACCTGCAATTGATTGTCGGGACCTTTTTCTGATCCGACATCCTGGGGCTGCGAAGCAGCCCCGGCAATCTCAAACCAACCCCAGCGCCCCCCGCCCCcgccgcccc > 2:527693/1‑133 (MQ=255) gCAGCTGGGTGGGCGGGATACCGGGACTTACCTGCAATTGATTGTCGGGACCTTTTTCTGATCCGACATCCTGGGGCTGCGAAGCAGCCCCGGCAAACTCAAACCAACCCCAGCTCCCCCCGCCCCccccgccccc > 2:347942/1‑132 (MQ=255) aGCTGGGTGGGCGGGATACCGGGACTTACCTGCAATTGATTGTCGGGACCTTTTTCTGATCCGACATCCTGGGGCTGCGAAGCAGCCCCGGCAATCTCAAACCAACCCCTGCTCCCCCAGCCCCCGCCGCCCGctc > 1:475298/1‑136 (MQ=255) aGCTGGGTGGGCGGGATACCGGGACTTACCTGCAATTGATTGTCGGGACCTTTTTCTGATCCGACATCCTGGGGCTGCGAAGCAGCCCCGGCAATCTCAAACCAACCCCTGCTCCCCCAGCCCCCGCCGCACGcgc > 2:480115/1‑134 (MQ=255) aGCTGGGTGGGCGGGATACCGGGACTTACCTGCAATTGATTGTCGGGACCTTTTTCTGATCCGACATCCTGGGGCTGCGAAGCAGCCCCGGCAATCTCAAACCAACCCCTGCGCCCCCCGCCCCCGccgcccgcgc > 2:354199/1‑134 (MQ=255) cTGGGTGGGCGGGATACCGGGACTTACCTGCAATTGATTGTCGGGACCTTTTTCTGATCCGACATCCTGGGGCTGCGAAGCAGCCCCGGCAATCTCAAACCAACCCCTGCTCCCCCAGCCACCGCCGCACGctcca > 1:1200658/1‑135 (MQ=255) cTTACCTGCAATTGATTGTCGGGACCTTTTTCTGATCCGACATCCTGGGGCTGCGAAGCAGCCCCGGCAATCTCAAACCAACCCCTGGTGCCCCCGCCCCCGCCGCCCGGGGCGCGGGTGGTGACCCGCCAGcccc > 1:1072226/1‑136 (MQ=255) aCCTGCAATTGATTGTCGGGACCTTTTTCTGATCCGACATCCTGGGGCTGCGAAGCAGCCCCGGCAATCTCAAACCAACCCCTTCACCCCCCCCCCCCCCCCCACGCACCGCTGCAGTTACCCAGCCAGccccccc > 2:319646/1‑133 (MQ=255) aCCTGCAATTGATTGTCGGGACCTTTTTCTGATCCGACATCCTGGGGCTGCGAAGCAGCCCCGGCAATCTCAAACCAACCCCAGCTCCCCCACCCACCGCCGCACGCGCCGCTGCTGTTGACCCCCCAGCCCCGAc > 2:1199017/1‑136 (MQ=255) ccTGCAATTGATTGTCGGGACCTTTTTCTGATCCGACATCCTGGGGCTGCGAAGCAGCCCCGGCAATCTCAAACCAACCCCTGCTCCCCCCGCCCCCGCCGCACGCTCCACTGCTGTTAACCCGCAAGCCCCgccc > 2:1032202/1‑133 (MQ=255) attgattgTCGGGACCTTTTTCTGATCCGACATCCTGGGGCTGCGAAGCAGCCCCGGCAATCTCAAACCAACCCCTGCTCCCCCACCCCCCGCCGCACGCGCCTCTGCTGTTCACCCCCAACCCCCGACAAAAccc > 1:734694/1‑135 (MQ=255) ttCTGATCCGACATCCTGGGGCTGCGAAGCAGCCCCGGCAATCACAAACCAAAACCAGCACCCCCACCCACCGCCCCCCCCACCTATGCTGTTAAACCGCAACCCCCCACAAAACAGCCGCAACAGCACCCCCAca > 2:111836/1‑135 (MQ=255) cTGATCCGACATCCTGGGGCTGCGAAGCAGCCCCGGCAATCTCAAACCAACCCCTGCTCCCCCAGCCACCGCCGCACGCTCCTCTGCTGTTCACCCGCAAGCCCCGACAATACCGCCTCAACAGGCCGCCCACTGc > 1:699226/1‑136 (MQ=255) cGACATCCTGGGGCTGCGAAGCAGCCCCGGCAATCTCAAACCAACCCCTGCTACCCCACCCACCCCCGCACGCGCCACTGCAGTTCACCCGCCAGCCCCGACAAAACCGCCGCAACAGGCCGCCCACTGCGGAttt > 1:317140/1‑136 (MQ=255) cccccccGGCAATCTCAAACCAACCCCTGCTCCCCCAGCCACCGCCGCACGCTCCTCTGCTGTTCACCCGCAAGCCCCGACAATACCGCCTCAACAGGCCGCCCACTGCGTATTTGTCGCACCACCGGCGCCAGtt < 1:1022853/133‑1 (MQ=255) cGGCAATCTCAAACCAACCCCTGCTCCCCCAGCCACCGCCGCACGCTCCTCTGCTGTTCACCCGCCAGCCCCGACAATACCGCCGCAACAGGCCGCCCACTGCGTATTTGTAGCACCACCGGCGCCAGTTCCAccc > 2:225513/1‑136 (MQ=255) cGGCAATCTCAAACCAACCCCTGCTCCCCCAGCCACCGCCGCACGCTCCTCTGCTGTTAACCCGCAAGCCCCGACAAAACCCCCACAACAGGCCGCCCACGGCGTATTTGTAGCACCACCGGCGCCAATTCCAccc > 2:626748/1‑136 (MQ=255) aGCCACCGCCGCACGCTCCTCTGCTGTTCACCCGCAAGCCCCGACAATACCGCCTCAACAGGCCGCCCACTGCGTATTTGTCGCACCACCGGCGCCAGTTCCACCCCCTGCAAATGCCAAACCCCCAGCGGCTGGt < 2:709926/136‑1 (MQ=255) aCCGCCGCACGCTCCTCTGCTGTTCACCCGCAAGCCCCGACAATACCGCCTCAACAGGCCGCCCACTGCGTATTTGTCGCACCACCGGCGCCAGTACCACCCCCTGCAAATGCCAAACCCCCAGCGGCTGGTccgc > 1:596310/1‑136 (MQ=255) | ATAGTGGTACGCAGCTGGGTGGGCGGGATACCGGGACTTACCTGCAATTGATTGTCGGGACCTTTTTCTGATCCGACATCCTGGGGCTGCGAAGCAGCCCCGGCAATCTCAAACCAACCCCTGCTCCCCCAGCCACCGCCGCACGCTCCTCTGCTGTTCACCCGCAAGCCCCGACAATACCGCCTCAACAGGCCGCCCACTGCGTATTTGTCGCACCACCGGCGCCAGTTCCACCCCCTGCAAATGCCAAACCCCCAGCGGCTGGTCCGC > NC_002947/3931673‑3931942 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |