Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947195,5590CA46.9% 9.2 / 35.5 33T355T (ACC→ACA) PP_0168putative surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base C (7/10);  new base A (10/5);  total (17/16)
Fisher's exact test for biased strand distribution p-value = 1.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.95e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

CCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACA  >  NC_002947/195432‑195689
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cccTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCGGCGTCACCATCGAGAACGCCACCGGCGGTaa                                                                                                                            >  2:890750/1‑136 (MQ=17)
            gCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACACCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGACACCATCGAGAACGCCACCGGCGCTAATTTCGAGCAACt                                                                                                                >  1:474320/1‑136 (MQ=255)
                     tcaCTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCTGTAATTTCGAGCAACTGACCCCGaa                                                                                                       <  1:770716/136‑1 (MQ=255)
                     tcaCTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGaa                                                                                                       <  1:932050/136‑1 (MQ=40)
                         tGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCg                                                                                                   >  1:638446/1‑136 (MQ=38)
                         tGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCg                                                                                                   <  1:429807/136‑1 (MQ=38)
                          gTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGa                                                                                                  <  1:122751/136‑1 (MQ=37)
                              aaGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAAtt                                                                                                                          >  2:775944/1‑108 (MQ=17)
                                                                 aCCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGAcaccc                                                           >  2:1313087/1‑135 (MQ=38)
                                                                 aCCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGAcaccc                                                           >  2:1342388/1‑135 (MQ=38)
                                                                        ccAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACcgc                                                    >  2:939852/1‑136 (MQ=39)
                                                                           aTGACGTCTACAACAACGGCTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGAc                                                 <  1:246568/136‑1 (MQ=37)
                                                                                     aaacaccGGTTGTACCGTAAGCTTCACCTTCTTTAAGGCAACGGGCGTTATTTTCTATCAAATGACCCCGAATGCGACCCGGGCTCTCCCCAGTAACCACGACTCGGTCGACACCACCACCGCGACCCTGACGGCg                                       <  2:474320/135‑1 (MQ=2)
                                                                                           cGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCg                                 >  1:409071/1‑136 (MQ=17)
                                                                                           cGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCg                                 >  1:343800/1‑136 (MQ=17)
                                                                                            ggTTCGACCGCCAGCGCCACCATCGACACCGCCACAGGCGGAAATTTCGACCAACTCACCCCGAATCCCACCCCGGCTCACCCCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGt                                <  1:986459/136‑1 (MQ=11)
                                                                                             gTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTc                               >  1:46158/1‑136 (MQ=37)
                                                                                             gTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTc                               >  1:713568/1‑136 (MQ=17)
                                                                                              ttCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCTCTACCGCGACCCTGCCGGCGAGCCCGTCg                              >  1:1119060/1‑136 (MQ=255)
                                                                                              ttCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCg                              >  2:684800/1‑136 (MQ=17)
                                                                                                           gTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAggcg                 <  1:607406/136‑1 (MQ=17)
                                                                                                             cactttCGACACCGCCCCCGGCGTAAATTCCGACCAACTGCCCCCGAATCCCACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAggcggc               <  1:989235/131‑1 (MQ=11)
                                                                                                             cACCATCGAGACCGCCACCGGCGTTAATTTCGACCAACTCCCCCCCAAACCCCCCCCGGCTCACACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAggcggc               <  1:1285239/136‑1 (MQ=11)
                                                                                                             cACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAggcggc               <  1:401518/136‑1 (MQ=17)
                                                                                                             cACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAggcggc               <  1:315889/136‑1 (MQ=17)
                                                                                                             cACCATCGACACCCCCACCGGGGTAAATTTCGAGCAACTGACCCCTAATCCGACCCCGGCTCACACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAggcggc               <  1:982376/136‑1 (MQ=11)
                                                                                                               ccacccagaACCCCACCGGCGTAAATTTCGACCAACTCCCCCCCAACCCCCCCCCGGCCCCGCCCCCGAACAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGt             <  1:890750/130‑1 (MQ=11)
                                                                                                                 aTCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGa           >  1:386559/1‑136 (MQ=17)
                                                                                                                 aTCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGa           >  1:354896/1‑136 (MQ=17)
                                                                                                                   ccccaaCCCCACCGGCGTTAATTTGGCCCCACTGCCCCCGAAACCGCCCCCGGCGCACACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATc         <  1:978848/132‑1 (MQ=11)
                                                                                                                       aaCGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACtt     >  1:1041348/1‑136 (MQ=17)
                                                                                                                       aaCGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACtt     >  2:150799/1‑136 (MQ=17)
                                                                                                                          gCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTaca  <  2:354896/136‑1 (MQ=17)
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CCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACA  >  NC_002947/195432‑195689

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑