| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,040,315 | 1 | . | G | 66.7% | 23.3 / 10.2 | 12 | coding (1130/1143 nt) | rffE | UDP‑N‑acetylglucosamine‑2‑epimerase |
| Reads supporting (aligned to +/- strand): ref base . (4/0); new base G (8/0); total (12/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCTTCGTCGTGATTGTTGACTGTTGTTGGGGGGGCACGCGGAAGGGGGGCTGCTGGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGAGCGGT‑‑GGG‑GG‑GGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAAGGTACTCAACGCCCG > NC_002947/2040187‑2040442 | tctTCGTCGTGATTGTTGACTGTTGTTGGGGGGGCACGCGGAAGGGGGGCTGCTGGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGAGCGGT‑‑ggg‑gg‑gg > 1:1177342/1‑136 (MQ=255) tgttgtTGGGGGGGCACGCGGAAGGGGGGCTGCTGGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTAGCGGGTGAACCCGCACCTACATTGAGCGGTG‑GGG‑GG‑GGGGGCCAGGTGGCCAGGGCt > 1:865482/1‑136 (MQ=255) ggggggCACGCGGAAGGGGGGCTGCTGGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTCAGCGGT‑‑GGGCGG‑GGGGGACAGGAGCCCAGGGCTTTGGGTAt > 2:34525/1‑136 (MQ=255) ggggggCACGCGGAAGGGGGGCTGCTGGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATAGAGAGGTG‑GGG‑GG‑GGGGGCCAGGTGGCCAGGGGTTTGCGTAt > 2:323047/1‑136 (MQ=255) aCGCGGAAGGGGGGCTGCTGGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGAGCGGT‑‑GGG‑GG‑GGGGGCCAGGTGGCCAGGGCTTTGCGTATgcgcgcgc > 1:173160/1‑136 (MQ=255) gggCTGCTGGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGAGCGGT‑‑GGG‑GG‑GGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCa > 2:513402/1‑136 (MQ=255) ggCCAGTTTTTACTGGGCGGCGGCGGGGCTGCGGATAACGTATTCGCGGGTGAACCCGCACCTACATTGAGCGGA‑‑GGG‑GGCGGGGGCAAGTTGGCCAGGGCTATGCTTAAGCCCGCCAAGGCTTTGCCATCGCCAt > 2:471398/1‑136 (MQ=255) ccAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGAGCGGTG‑GGG‑GG‑GGGGGCCAGGTGGCCAGGGCTTTGCTTATGCGCGCGCAGGATTTGCAATCCCCATAg > 2:1044007/1‑136 (MQ=255) cgcgGGTGAACCCGCACCTACATTGAGCGGTGGGGG‑GG‑GGGGGCCAGGTGGCCAGGGCTTTGGGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTTTGGGGGTCGGTAATTTCGCGATACGCCGCCGCATc > 2:979585/1‑136 (MQ=255) gTGAACCCGCACCTACATTGAGCGGTG‑GGG‑GG‑GGGGGCCGGGTGGCCAGGGCTTTGCTTATGCGCGCGCAGGGTTTGCAATCGCCTTAGGGGTTATGGGCGAAGCTCATTAGGCGATACGCCGCCTCAACGGTCAg > 2:1241279/1‑136 (MQ=255) ccGCACCTACATTGAGCGGTG‑GGG‑GG‑GGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAAGGt > 1:558230/1‑136 (MQ=255) ttGAGCGGTG‑GGG‑GG‑GGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAAGGTACTCAACGccc > 2:1025943/1‑136 (MQ=255) tGAGCGGTG‑GGG‑GG‑GGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAAGGTACTCAACGcccc > 1:1087566/1‑135 (MQ=255) | TCTTCGTCGTGATTGTTGACTGTTGTTGGGGGGGCACGCGGAAGGGGGGCTGCTGGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGAGCGGT‑‑GGG‑GG‑GGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAAGGTACTCAACGCCCG > NC_002947/2040187‑2040442 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |