Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	3,824,314	C→G	A279P (GCG→CCG)	kguT ←	2‑ketogluconate transporter, putative

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,824,314	0	C	G	100.0%	146.7 / NA	40	A279P (GCG→CCG)	kguT	2‑ketogluconate transporter, putative
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (16/24);  total (16/24)

CCAAGGCGTAGGAACCATAGAACGCCAACGCGGCAATCAGCAGCGGCGGCCAGACGAAGCGCTTGCGCTTTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGCCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAGCCACAGCACGAAGCCATACACGCCGATGCTCCAGCAGAAGTACTGCAGCGCCAGGAT  >  NC_002947/3824194‑3824446                                                                                                                         |                                                                                                                                     ccAAGGCGTAGGAACCATAGAACGCCAACGCGGCAATCAGCAGCGGCGGCCAGACGAAGCGCTTGCGCTTTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGa                                                                                                                       >  1:280755/1‑136 (MQ=255)        gTAGGAACCATAGAACGCCAACGCGGCAATCAGCAGCGGCGGCCAGACGAAGCGCTTGCGCTTTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGAGgccagc                                                                                                                <  2:322186/136‑1 (MQ=255)                cATAGAACGCCAACGCGGCAATCAGCAGCGGCGGCCAGACGAAGCGCTTGCGCTTTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGc                                                                                                        <  2:496842/136‑1 (MQ=255)                  tAGAACGCCAACGCGGCAATCAGCAGCGGCGGCCAGACGAAGCGCTTGCGCTTTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGccg                                                                                                      >  1:1306090/1‑135 (MQ=255)                  tAGAACGCCAACGCGGCAATCAGCAGCGGCGGCCAGACGAAGCGCTTGCGCTTTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCt                                                                                                      <  1:484215/136‑1 (MQ=255)                  tAGAACGCCAACGCGGCAATCAGCAGCGGCGGCCAGACGAAGCGCTTGCGCTTTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCt                                                                                                      <  1:420210/136‑1 (MQ=255)                  tAGAACGCCAACGCGGCAATCAGCAGCGGCGGCCAGACGAAGCGCTTGCGCTTTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCt                                                                                                      <  1:67995/136‑1 (MQ=255)                  tAGAACGCCAACGCGGCAATCAGCAGCGGCGGCCAGACGAAGCGCTTGCGCTTTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCt                                                                                                      <  1:41476/136‑1 (MQ=255)                  tAGAACGCCAACGCGGCAATCAGCAGCGGCGGCCAGACGAAGCGCTTGCGCTTTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCt                                                                                                      >  1:540444/1‑136 (MQ=255)                          cAACGCGGCAATCAGCAGCGGCGGCCAGACGAAGCGCTTGCGCTTTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGata                                                                                              >  1:1277110/1‑136 (MQ=255)                          cAACGCGGCAATCAGCAGCGGCGGCCAGACGAAGCGCTTGCGCTTTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGata                                                                                              >  1:137828/1‑136 (MQ=255)                                ggCAATCAGCAGCGGCGGCCAGACGAAGCGCTTGCGCTTTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACg                                                                                        >  1:191120/1‑136 (MQ=255)                                      cagcagCGGCGGCCAGACGAAGCGCTTGCGCTTTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTcgcc                                                                                  <  1:630619/136‑1 (MQ=255)                                      cagcagCGGCGGCCAGACGAAGCGCTTGCGCTTTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTcgcc                                                                                  <  1:357337/136‑1 (MQ=255)                                            cggcggCCAGACGAAGCGCTTGCGCTTTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGc                                                                            <  2:280755/136‑1 (MQ=255)                                                  ccAGACGAAGCGCTTGCGCTTTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAgg                                                                      >  2:264771/1‑136 (MQ=255)                                                      aCGAAGCGCTTGCGCTTTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCg                                                                  <  2:1306090/136‑1 (MQ=255)                                                      aCGAAGCGCTTGCGCTTTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCg                                                                  <  2:540444/136‑1 (MQ=255)                                                        gAAGCGCTTGCGCTTTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGAc                                                                <  2:818943/136‑1 (MQ=255)                                                             gcttgcgcttTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAg                                                           <  1:44057/136‑1 (MQ=255)                                                             gcttgcgcttTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAg                                                           <  1:524764/136‑1 (MQ=255)                                                                  cgcttTTGCAGGCGGTCGGACGCCCAGGACTCGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAGCcaca                                                      <  2:789843/136‑1 (MQ=255)                                                                    cttTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAGCCACAGc                                                    <  1:1147727/136‑1 (MQ=255)                                                                    cttTTGCAGGCGGTCGGACGCCAAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAGCCACAGc                                                    <  1:287788/136‑1 (MQ=255)                                                                       ttGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAGCCACAGCACg                                                 <  1:1372710/136‑1 (MQ=255)                                                                          cAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAGCCACAGCACGAAg                                              >  1:1114574/1‑136 (MQ=255)                                                                                gTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAGCCACAGCACGAAGCCATac                                        >  2:265577/1‑136 (MQ=255)                                                                                gTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGAAATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAGCCACAGCACGAAGCCATac                                        >  2:526608/1‑136 (MQ=255)                                                                                    gACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAGCCACAGCACGAAGCCATACACGc                                    <  2:1114574/136‑1 (MQ=255)                                                                                            ggACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAGCCACAGCACGAAGCCATACACGCCGATGCTc                            <  2:1277110/136‑1 (MQ=255)                                                                                            ggACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTGCGCCGCCTGCTGGAGGATCGCCGGCAGCCACAGCACGAAGCCATACACGCCGATGCTc                            <  2:327478/136‑1 (MQ=255)                                                                                                     caccaTTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAGCCACAGCACGAAGCCATACACGCCGATGCTCCAGCAGAAg                   >  2:852972/1‑136 (MQ=255)                                                                                                     caccaTTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAGCCACAGCACGAAGCCATACACGCCGATGCTCCAGCAGAAg                   >  2:280424/1‑136 (MQ=255)                                                                                                     caccaTTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAGCCACAGCACGAAGCCATACACGCCGATGCTCCAGCAGAAg                   >  1:175620/1‑136 (MQ=255)                                                                                                       ccaTTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAGCCACAGCACGAAGCCATACACGCCGATGCTCCAGCAGAAGTa                 <  2:28036/136‑1 (MQ=255)                                                                                                             ccAGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAGCCACAGCACGAAGCCATACACGCCGATGCTCCAGCAGAAGTACTGCAg           <  1:900159/136‑1 (MQ=255)                                                                                                               aGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAGCCACAGc                                                    <  2:324880/93‑1 (MQ=255)                                                                                                               aGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAGCCACAGc                                                    >  1:324880/1‑93 (MQ=255)                                                                                                               aGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAGCCACAGCACGAAGCCATACACGCCGATGCTCCAGCAGAAGTACTGCAgcg         >  1:14273/1‑136 (MQ=255)                                                                                                               aGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAGCCACAGCACGAAGCCATACACGCCGATGCTCCAGCAGAAGTACTGCAgcc         >  2:479981/1‑135 (MQ=255)                                                                                                                    cgccggCTGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAGCCACAGCACGAAGCCATACACGCCGATGCTCCAGCAGAAGTACTGCAGCGCCAgg    <  1:100660/136‑1 (MQ=255)                                                                                                                    cgccggCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAGCCACAGCACGAAGCCATACACGCCGATGCTCCAGCAGAAGTACTGCAGCGCCAgg    >  1:949729/1‑136 (MQ=255)                                                                                                                      ccggcAGGTATGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGCATCGACGGCAGCCACAGCACGAAGCCATACACGCCGATGCTCCAGCAGAAGTACTGCAGCGCCAGgat  <  2:137828/136‑1 (MQ=255)                                                                                                                         |                                                                                                                                     CCAAGGCGTAGGAACCATAGAACGCCAACGCGGCAATCAGCAGCGGCGGCCAGACGAAGCGCTTGCGCTTTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGCCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAGCCACAGCACGAAGCCATACACGCCGATGCTCCAGCAGAAGTACTGCAGCGCCAGGAT  >  NC_002947/3824194‑3824446

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑