Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	4,637,297	A→G	S143P (TCT→CCT)	uvrY ←	BarA/UvrY two‑componenent system response regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,637,297	0	A	G	100.0%	125.6 / NA	34	S143P (TCT→CCT)	uvrY	BarA/UvrY two‑componenent system response regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/25);  total (9/25)

AAGATCCGATAACGGTAAGTATTGACGGTCTTGGGCGAGAGGCACAACTTGTCGGAGATGATCTGCACTTTCTGGCAGCCGACGATCATCAGGGCAATCTGGATTTCCCGTTCGGACAACGCGTCGAACGGAGACCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAG  >  NC_002947/4637164‑4637420                                                                                                                                      |                                                                                                                            aaGATCCGATAACGGTAAGTATTGACGGTCTTGGGCGAGAGGCACAACTTGTCGGAGATGATCTGCACTTTCTGGCAGCCGACGATCATCAGGGCAATCTGGATTTCCCGTTCGGACAACGCGTCGAACGGAGGcc                                                                                                                           <  2:931898/136‑1 (MQ=255)  aGATCCGATAACGGTAAGTATTGACGGTCTTGGGCGAGAGGCACAACTTGTCGGAGATGATCTGCACTTTCTGGCAGCCGACGATCATCAGGGCAATCTGGATTTCCCGTTCGGACAACGCGTCGAACGGAGGccc                                                                                                                          <  1:713873/136‑1 (MQ=255)           aaCGGTAAGTATTGACGGTCTTGGGCGAGAGGCACAACTTGTCGGAGATGATCTGCACTTTCTGGCAGCCGACGATCATCAGGGCAATCTGGATTTCCCGTTCGGACAACGCGTCGAACGGAGGCCCCTGCGGCTg                                                                                                                 <  2:877449/136‑1 (MQ=255)                   gTATTGACGGTCTTGGGCGAGAGGCACAACTTGTCGGAGATGATCTGCACTTTCTGGCAGCCGACGATCATCAGGGCAATCTGGATTTCCCGTTCGGACAACGCGTCGAACGGAGGCCCCTGCGGCTGGAACGGtt                                                                                                         <  1:1047887/136‑1 (MQ=255)                   gTATTGACGGTCTTGGGCGAGAGGCACAACTTGTCGGAGATGATCTGCACTTTCTGGCAGCCGACGATCATCAGGGCAATCTGGATTTCCCGTTCGGACAACGCGTCGAACGGAGGCCCCTGCGGCTGGAACGGtt                                                                                                         <  1:569580/136‑1 (MQ=255)                           ggTCTTGGGCGAGATGCACAACTTGTCGGAGATGATCTGCACTTTCTGGCAGCAGACGATCATCAGGGCAATCTGGATTTCCCGTTCGGACAACGCGTCGAACGGAGGCCCCTGCGGCAGGAACTGTTTCAGCGcc                                                                                                 >  2:628578/1‑136 (MQ=255)                           ggTCTTGGGCGAGAGGCACAACTTGTCGGAGATGATCTGCACTTTCTGGCAGCCGACGATCATCAGGGCAATCTGGATTTCCCGTTCGGACAACGCGTCGAACGGAGGCCCCTGCGGCTGGAACGGTTTCAGCGcc                                                                                                 >  2:685343/1‑136 (MQ=255)                                 gggCGAGAGGCACAACTTGTCGGAGATGATCTGCACTTTCTGGCAGCCGACGATCATCAGGGCAATCTGGATTTCCCGTTCGGACAACGCGTCGAACGGAGGCCCCTGCGGCTGGAACGGTTTCAGCGCCAActgc                                                                                           >  1:1313209/1‑136 (MQ=255)                                 gggCGAGAGGCACAACTTGTCGGAGATGATCTGCACTTTCTGGCAGCCGACGATCATCAGGGCAATCTGGATTTCCCGTTCGGACAACGCGTCGAACGGAGGCCCCTGCGGCTGGAACGGTTTCAGCGCCAActgc                                                                                           >  1:373404/1‑136 (MQ=255)                                         ggCACAACTTGTCGGAGATGATCTGCACTTTCTGGCAGCCGACGATCATCAGGGCAATCTGGATTTCCCGTTCGGACAACGCGTCGAACGGAGGCCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGAt                                                                                   <  2:803909/136‑1 (MQ=255)                                         ggCACAACTTGTCGGAGATGATCTGCACTTTCTGGCAGCCGACGATCATCAGGGCAATCTGGATTTCCCGTTCGGACAACGCGTCGAACGGAGGCCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGAt                                                                                   <  1:516789/136‑1 (MQ=255)                                         ggCACAACTTGTCGGAGATGATCTGCACTTTCTGGCAGCCGACGATCATCAGGGCAATCTGGATTTCCCGTTCGGACAACGCGTCGAACGGAGGCCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGAt                                                                                   <  2:1313209/136‑1 (MQ=255)                                          gCACAACTTGTCGGAGATGATCTGCACTTTCTGGCAGCCGACGATCATCAGGGCAATCTGGATTTCCCGTTCGGACAACGCGTCGAACGGAGGCCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATc                                                                                  >  1:867713/1‑136 (MQ=255)                                              aaCTTGTCGGAGATGATCTGCACTTTCTGGCAGCCGACGATCATCAGGGCAATCTGGATTTCCCGTTCGGACAACGCGTCGAACGGAGGCCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATCTGCg                                                                              <  1:1060979/136‑1 (MQ=255)                                              aaCTTGTCGGAGATGATCTGCACTTTCTGGCAGCCGACGATCATCAGGGCAATCTGGATTTCCCGTTCGGACAACGCGTCGAACGGAGGCCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATCTGCg                                                                              <  2:950596/136‑1 (MQ=255)                                              aaCTTGTCGGAGATGATCTGCACTTTCTGGCAGCCGACGATCATCAGGGCAATCTGGATTTCCCGTTCGGACAACGCGTCGAACGGAGGCCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATCTGCg                                                                              <  2:472719/136‑1 (MQ=255)                                               aCTTGTCGGAGATGATCTGCACTTTCTGGCAGCCGACGATCATCAGGGCAATCTGGATTTCCCGTTCGGACAACGCGTCGAACGGAGGCCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATCTGCgg                                                                             >  1:239717/1‑136 (MQ=255)                                                   gTCGGAGATGATCTGCACTTTCTGGCAGCCGACGATCATCAGGGCAATCTGGATTTCCCGTTCGGACAACGCGTCGAACGGAGGCCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATCTGCGGGCTg                                                                         <  2:1139989/136‑1 (MQ=255)                                                   gTCGGAGATGATCTGCACTTTCTGGCAGCCGACGATCATCAGGGCAATCTGGATTTCCCGTTCGGACAACGCGTCGAACGGAGGCCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATCTGCGGGCTg                                                                         <  2:965574/136‑1 (MQ=255)                                                                          ggCAGCCGACGATCATCAGGGCAATCTGGATTTCCCGTTCGGACAACGCGTCGAACGGAGGCCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGc                                                  >  2:1358951/1‑136 (MQ=255)                                                                          ggCAGCCGACGATCATCAGGGCAATCTGGATTTCCCGTTCGGACAACGCGTCGAACGGAGGCCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGc                                                  >  2:1364751/1‑136 (MQ=255)                                                                              gCCGACGATCATCAGGGCAATCTGGATTTCCCGTTCGGACAACGCGTCGAACGGAGGCCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGa                                              <  2:99068/136‑1 (MQ=255)                                                                              gCCGACGATCATCAGGGCAATCTGGATTTCCCGTTCGGACAACGCGTCGAACGGAGGCCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGa                                              <  2:1251216/136‑1 (MQ=255)                                                                              gCCGACGATCATCAGGGCAATCTGGATTTCCCGTTCGGACAACGCGTCGAACGGAGGCCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGa                                              <  1:1373470/136‑1 (MQ=255)                                                                              gCCGACGATCATCAGGGCAATCTGGATTTCCCGTTCGGACAACGCGTCGAACGGAGGCCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGa                                              <  1:294217/136‑1 (MQ=255)                                                                               ccGACGATCATCAGGGCAATCTGGATTTCCCGTTCGGACAACGCGTCGAACGGAGGCCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGAc                                             <  1:1364751/136‑1 (MQ=255)                                                                               ccGACGATCATCAGGGCAATCTGGATTTCCCGTTCGGACAACGCGTCGAACGGAGGCCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGAc                                             <  1:1358951/136‑1 (MQ=255)                                                                                       catcaGGGCAATCTGGATTTCCCGTTCGGACAACGCGTCGAACGGAGGCCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGcc                                     <  2:729743/136‑1 (MQ=255)                                                                                                  ccTGGATTTCCCGTTCGGACAACGCGTCGAACGGAGGCCCCTGCGGCTGGAACGGTTGCAGCGCCAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTc                          <  1:289609/135‑1 (MQ=255)                                                                                                  acTGGATTTCCCGTTCGGACAACGCGTCGAACGGAGGCCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTc                          <  2:1358499/135‑1 (MQ=255)                                                                                                          tCCCGTTCGGACAACGCGTCGAACGGAGGCCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGc                  <  2:709574/136‑1 (MQ=255)                                                                                                              gTTCGGACAACGCGTCGAACGGAGGCCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGc                  >  2:650422/1‑132 (MQ=255)                                                                                                              gTTCGGACAACGCGTCGAACGGAGGCCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGc                  <  1:650422/132‑1 (MQ=255)                                                                                                                          cgTCGAACGGAGGCCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAg  <  2:867713/136‑1 (MQ=255)                                                                                                                                      |                                                                                                                            AAGATCCGATAACGGTAAGTATTGACGGTCTTGGGCGAGAGGCACAACTTGTCGGAGATGATCTGCACTTTCTGGCAGCCGACGATCATCAGGGCAATCTGGATTTCCCGTTCGGACAACGCGTCGAACGGAGACCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAG  >  NC_002947/4637164‑4637420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑