Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 4,743,205 C→G intergenic (+474/+278) gltA → / ← yeiW citrate synthase/putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,743,2050CG97.2% 127.9 / NA 36intergenic (+474/+278)gltA/yeiWcitrate synthase/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base G (19/16);  total (20/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑AACCTCCGCCAAC‑TGCATTCGTGAACCAAGCAACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCC  >  NC_002947/4743092‑4743320
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gCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGGCACCTTCGTGCTTGAGCAGCTTCTGCGACT‑TGCATTCGTGaatccagg                                                                                                <  2:91662/136‑7 (MQ=255)
            gTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACT‑TGCATTCGTGAATCCAGGCACAGGCCCCgg                                                                                    <  1:135637/136‑1 (MQ=255)
              cGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACT‑TGCATTCGTGAATCCAGGCACAGGCCCCGGac                                                                                  <  1:660599/136‑1 (MQ=255)
              cGTAATGAGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACT‑TGCATTCGTGAATCCAGGCACAGGCCCCGGac                                                                                  <  2:1294829/136‑1 (MQ=255)
               gTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACT‑TGCATTCGTGAATCCAGGCACAGGCCCCGGaca                                                                                 >  1:921730/1‑136 (MQ=255)
               gTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACT‑TGCATTCGTGAATCCAGGCACAGGCCCCGGaca                                                                                 >  1:554983/1‑136 (MQ=255)
                  aTGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACT‑TGCATTCGTGAATCCAGGCACAGGCCCCGGACACGt                                                                              >  1:1262084/1‑136 (MQ=255)
                              gAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACT‑TGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAgcg                                                                  >  2:679239/1‑136 (MQ=255)
                              gAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACT‑TGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAgcg                                                                  <  1:726329/136‑1 (MQ=255)
                                  cACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACT‑TGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTc                                                              >  1:905258/1‑136 (MQ=255)
                                            cACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACT‑TGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGcc                                                    >  1:1247698/1‑136 (MQ=255)
                                             aCCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACT‑TGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCa                                                   <  2:1208203/136‑1 (MQ=255)
                                                            ttGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACT‑TGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCa                                    >  1:1152405/1‑136 (MQ=255)
                                                                 cTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACT‑TGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTc                               <  1:1094299/136‑1 (MQ=255)
                                                                 cTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACT‑TGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTc                               <  1:999061/136‑1 (MQ=255)
                                                                 cTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACT‑TGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTc                               <  1:1084607/136‑1 (MQ=255)
                                                                  tGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACT‑TGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTca                              <  2:1262084/136‑1 (MQ=255)
                                                                    ggTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACT‑TGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTcaca                            >  2:1326037/1‑136 (MQ=255)
                                                                       ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACT‑TGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAgcggc                                              >  1:1098152/1‑115 (MQ=255)
                                                                       ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACT‑TGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAgcggc                                              <  2:1098152/115‑1 (MQ=255)
                                                                       ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACT‑TGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcg                         >  1:757731/1‑136 (MQ=255)
                                                                       ataGGTCCAGCTCTGGTCACCATCGTGCTTTAGCAGCTTCTGCGACT‑TTCATTCGTGAATCCAGGCACAGTCACCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGACTTGATCACGGTCACAccc                         >  1:847562/1‑135 (MQ=255)
                                                                        taGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACT‑TGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                        <  1:639019/136‑1 (MQ=255)
                                                                        taGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACT‑TGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                        <  2:921730/136‑1 (MQ=255)
                                                                        taGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACT‑TGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                        <  1:729924/136‑1 (MQ=255)
                                                                                 ctctGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACT‑TGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGcca               >  1:1236861/1‑135 (MQ=255)
                                                                                 ctctGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACT‑TGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGcca               >  1:176733/1‑135 (MQ=255)
                                                                                 ctctGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACT‑TGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCt               >  2:1077631/1‑136 (MQ=255)
                                                                                 ctctGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACT‑TGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCt               >  1:1120055/1‑136 (MQ=255)
                                                                                 ctctGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACT‑TGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCt               >  1:983995/1‑136 (MQ=255)
                                                                                 ctctGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACT‑TGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCt               >  2:944430/1‑136 (MQ=255)
                                                                                        cACCTTCGTGCTTGAGCAGCTTCTGCGACT‑TGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACtt        >  2:278525/1‑136 (MQ=255)
                                                                                         aCCTTCGTGCTTGAGCAGCTTCTGCGACT‑TGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTg       <  2:267030/136‑1 (MQ=255)
                                                                                         aCCTTCGTGCTTGAGCAGCTTCTGCGACT‑TGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTg       <  2:512928/136‑1 (MQ=255)
                                                                                         aCCTTCGTGCTTGAGCAGCTTCTGCGACT‑TGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTg       >  1:179978/1‑136 (MQ=255)
                                                                                              cGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGAccc  >  2:1075219/1‑136 (MQ=255)
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GCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑AACCTCCGCCAAC‑TGCATTCGTGAACCAAGCAACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCC  >  NC_002947/4743092‑4743320

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑