Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	5,367,931	G→A	P199S (CCC→TCC)	ftsH ←	integral membrane ATP‑dependent zinc metallopeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,367,931	0	G	A	100.0%	192.8 / NA	54	P199S (CCC→TCC)	ftsH	integral membrane ATP‑dependent zinc metallopeptidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/31);  total (23/31)

GAACATGTCGCGCACACGGCTGGCACCGACACCCACGAACATTTCCACGAAGTCCGAACCGGAAATGGTGAAGAACGGTACTTTCGCTTCGCCCGCGATGGCCTTGGCCAGCAGGGTCTTACCGGTACCGGGTGGGCCGACCATCAGCACACCGCGCGGGATACGGCCACCCAGGCGCTGGAACTTGCCCGGATCGCGCAGGAACTCGACCAGCTCGCCAACCTCTTCCTTGGCCTCGTCGCAGCCCGCAACGTCAGCCAGCG  >  NC_002947/5367800‑5368062                                                                                                                                    |                                                                                                                                    gAACATGTCGCGCACACGGCTGGCACCGACACCCACGAACATTTCCACGAAGTCCGAACCGGAAATGGTGAAGAACGGTACTTTCTCTTCGCCCGCGATGGCCTTGGCCAGCAGGGTCTTACCGGTACCTGATggg                                                                                                                                 >  2:468184/1‑136 (MQ=255) gAACATGTCGCGCACACGGCTGGCACCGACACCCACGAACATTTCCACGAAGTCCGAACCGGAAATGGTGAAGAACGGTACTTTCGCTTCGCCCGCGATGGCCTTTGCCAGCAGGGTCTTACCGGTACCGGATggg                                                                                                                                 <  1:444391/136‑1 (MQ=255) gAACATGTCGCGCACACGGCTGGCACCGACACCCACGAACATTTCCACGAAGTCCGAACCGGAAATGGTGAAGAACGGTACTTTCGCTTCGCCCGCGATGGCCTTGGCCAGCAGGGTCTTACCGGTACCGGATggg                                                                                                                                 >  1:296565/1‑136 (MQ=255) gAACATGTCGCGCACACGGCTGGCACCGACACCCACGAACATTTCCACGAAGTCCGAACCGGAAATGGTGAAGAACGGTACTTTCGCTTCGCCCGCGATGGCCTTGGCCAGCAGGGTCTTACCGGTACCGGATggg                                                                                                                                 >  1:1359135/1‑136 (MQ=255) gAACATGTCGCGCACACGGCTGGCACCGACACCCACGAACATTTCCACGAAGTCCGAACCGGAAATGGTGAAGAACGGTACTTTCGCTTCGCCCGCGATGGCCTTGGCCAGCAGGGTCTTACCGGTACCGGATggg                                                                                                                                 <  1:242970/136‑1 (MQ=255) gAACATGTCGCGCACACGGCTGGCACCGACACCCACGAACATTTCCACGAAGTCCGAACCGGAAATGGTGAAGAACGGTACTTTCGCTTCGCCCGCGATGGCCTTGGCCAGCAGGGTCTTACCGGTACCGGATggg                                                                                                                                 >  2:270246/1‑136 (MQ=255) gAACATGTCGCGCACACGGCTGGCACCGACACCCACGAACATATCCACGAAGTCCGAACCGGAAATGGTGAAGAACGGTACTTTCGCTTCGCCCGCGATGGCCTTGGCCAGCAGGGTCTTACCGGTACCGGATggg                                                                                                                                 <  2:532020/136‑1 (MQ=255) gAACATGTCGCGCACACGGCTGGCACCGACACCCACGAACAGTTCCACGAAGTCCGAACCGGAAATGGTGAAGAACGGTACTTTCGCTTCGCCCGCGATGGCCTTGGCCAGCAGGGTCTTACCGGTACCGGATggg                                                                                                                                 >  2:1306409/1‑136 (MQ=255)   aCATGTCGCGCACACGGCTGGCACCGACACCCACGAACATTTCCACGAAGTCCGAACCGGAAATGGTGAAGAACGGTACTTTCGCTTCGCCCGCGATGGCCTTGGCCAGCAGGGTCTTACCGGTACCGGATGGGcc                                                                                                                               <  2:695564/136‑1 (MQ=255)   aCATGTCGCGCACACGGCAGGCACCGACACCCACGAACATTTCCACGAAGTCCGAACCGGAAATGGTGAAGAACGGTACTTTCGCTTCGCCCGCGATGGCCTTGGCCAGCAGGGTCTTACCGGTACCGGATGGGcc                                                                                                                               <  2:1118996/136‑1 (MQ=255)   aCAGGTCGCGCACACGGCTGGCACCGACACCCACGAACATTTCCACGAAGTCCGAACCGGAAATGGTGAAGAACGGTACTTTCGCTTCGCCCGCGATGGCCTTGGCCAGCAGGGTCTTACCGGTACCGGATGGGcc                                                                                                                               <  2:1125448/136‑1 (MQ=255)              acacGGCTGGCACCGACACCCACGAACATTTCCACGAAGTCCGAACCGGAAATGGTGAAGAACGGTACTTTCGCTTCGCCCGCGATGGCCTTGGCCAGCAGGGTCTTACCGGTACCGGATGGGCCGACCATCAGca                                                                                                                    >  2:517479/1‑136 (MQ=255)                  ggCTGGCACCGACACCCACGAACATTTCCTCGAAGTCCGAACCGGATATGGTGAAGAACGGTACTTTCGCTTCGCCCGCGATGGCCTTGGCCAGCAGGGTCTTACCGGTACCGGATGGGCCGACCATCAGCACAcc                                                                                                                <  2:357191/136‑1 (MQ=255)                  ggCTGGCACCGACACCCACGAACATTTCCACGAAGTCCGAACCGGAAATGGTGAAGAACGGTACTTTCGCTTCGCCCGCGATGGCCTTGGCCAGCAGGGTCTTACCGGTACCGGATGGGCCGACCATCAGCACAcc                                                                                                                <  2:68257/136‑1 (MQ=255)                     tGGCACCGACACCCACGAACATTTCCACGAAGTCCGAACCGGAAATGGTGAAGAACGGTACTTTCGCTTCGCCCGCGATGGCCTTGGCCAGCAGGGTCTTACCGGTACCGGATGGGcc                                                                                                                               <  2:688545/118‑1 (MQ=255)                     tGGCACCGACACCCACGAACATTTCCACGAAGTCCGAACCGGAAATGGTGAAGAACGGTACTTTCGCTTCGCCCGCGATGGCCTTGGCCAGCAGGGTCTTACCGGTACCGGATGGGcc                                                                                                                               >  1:688545/1‑118 (MQ=255)                     tGGCACCGACACCCACGAACATTTCCACGAAGTCCGAACCGGAAATGGTGAAGAACGGTACTTTCGCTTCGCCCGCGATGGCCTTGGCCAGCAGGGTCTTACCGGTACCGGATGGGCCGACCATCAGCACAcc                                                                                                                <  1:731315/133‑1 (MQ=255)                     tGGCACCGACACCCACGAACATTTCCACGAAGTCCGAACCGGAAATGGTGAAGAACGGTACTTTCGCTTCGCCCGCGATGGCCTTGGCCAGCAGGGTCTTACCGGTACCGGATGGGCCGACCATCAGCACAcc                                                                                                                >  2:731315/1‑133 (MQ=255)                           cGACACCCACGAACATTTCCACGAAGTCCGAACCGGAAATCGTGAAGAACGGTACTTTCGCTTCGCCCGCGATGGCCTTGGCCAGCAGGGTCTTACCGGTACCGGATGGGCCGACCATCAGCACACCGCGCGGGAt                                                                                                       <  2:456200/136‑1 (MQ=255)                             acacCCACGAACATTTCCACGAAGTCCGAACCGGAAATGGTGAAGAACGGTACTTTCGCTTCGCCCGCGATGGCCTTGGCCAGCAGGGTCTTACCGGTACCGGATGGGCCGACCATCAGCACACCGCGCGGGATAc                                                                                                     <  1:270246/136‑1 (MQ=255)                               acCCACGAACATTTCCACGAAGTCCGAACCGGAAATGGTGAAGAACGGTACTTTCGCTTCGCCCGCGATGGCCTTGGCCAGCAGGGTCTTACCGGTACCGGATGGGCCGACCATCAGCACACCGCGCGGGATACgg                                                                                                   >  1:1325454/1‑136 (MQ=255)                               acCCACGAACATTTCCACGAAGTCCGAACCGGAAATGGTGAAGAACGGTACTTTCGCTTCGCCCGCGATGGCCTTGGCCAGCAGGGTCTTACCGGTACCGGATGGGCCGACCATCAGCACACCGCGCGGGATACgg                                                                                                   >  1:212960/1‑136 (MQ=255)                                    gggtcATTTGCACGAAGTCCGATCGGGATATGGTGTAGAACGGTACTTTCGCTTCGCCCGCGATGGCCTTGGCCAGCAGGGTGTTACGGGTACCGGATGTGCCGACCATCAGGACACGGGGCGGGATTCGGCCTcc                                                                                              <  1:272220/132‑1 (MQ=255)                                    cggaCATTTCCACGAAGTCCGAACCGGATATGGTGAAGAACGGTACTTTCGCTTCGCCCGCGATGGCCTTGGCCAGCAGGGTCTTACCGGTACCGGATGTGCCGACCATCAGCACACCGCGCGGGATACGGCCAcc                                                                                              <  1:265932/133‑1 (MQ=255)                                       aCATTTCCACGAAGTCCGAACCGGAAATGGTGAAGAACGGTACTTTCGCTTCGCCCGCGATGGCCTTGGCCAGCAGGGTCTTACCGGTACCGGATGGGCCGACCATCAGCACACCGCGCGGGATACGGCCACCCAg                                                                                           >  2:1198506/1‑136 (MQ=255)                                           ttCCACGAAGTCCGAACCGGAAATGGTGAAGAACGGTACTTTCGCTTCGCCCGCGATGGCCTTGGCCAGCAGGGTCTTACCGGTACCGGATGGGCCGACCATCAGCACAcc                                                                                                                >  2:618217/1‑111 (MQ=255)                                           ttCCACGAAGTCCGAACCGGAAATGGTGAAGAACGGTACTTTCGCTTCGCCCGCGATGGCCTTGGCCAGCAGGGTCTTACCGGTACCGGATGGGCCGACCATCAGCACAcc                                                                                                                <  1:618217/111‑1 (MQ=255)                                                              gAAATGGTGAAGAACGGTACTTTCGCTTCGCCCGCGATGGCCTTGGCCAGCAGGGTCTTACCGGTACCGGATGGGCCGACCATCAGCACACCGCGCGGGATACGGCCACCCAGGCGCTGGAACTTGCCCGGATcgc                                                                    <  2:99830/136‑1 (MQ=255)                                                              gAAATGGTGAAGAACGGTACTTTCGCTTCGCCCGCGATGGCCTTGGCCAGCAGGGTCTTACCGGTACCGGATGGGCCGACCATCAGCACACCGCGCGGGATACGGCCACCCAGGCGCTGGAACTTGCCCGGATcgc                                                                    <  1:517479/136‑1 (MQ=255)                                                                 aTGGTGAAGAACGGTACTTTCGCTTCGCCCGCGATGGCCTTGGCCAGCAGGGTCTTACCGGTACCGGATGGGCCGACCATCAGCACACCGCGCGGGATACGGCCAccc                                                                                             >  2:1210651/1‑108 (MQ=255)                                                                 aTGGTGAAGAACGGTACTTTCGCTTCGCCCGCGATGGCCTTGGCCAGCAGGGTCTTACCGGTACCGGATGGGCCGACCATCAGCACACCGCGCGGGATACGGCCAccc                                                                                             <  1:1210651/108‑1 (MQ=255)                                                                  tGGTGAAGAACGGTACTTTCGCTTCGCCCGCGATGGCCTTGGCCAGCAGGGTCTTACCGGTACCGGATGGGCCGACCATCAGCACACCGCGCGGGATACGGCCACCCAGGCGCTGGAACTTGCCCGGATCGCGCAg                                                                >  1:225111/1‑136 (MQ=255)                                                                  tGGTGAAGAACGGTACTTTCGCTTCGCCCGCGATGGCCTTGGCCAGCAGGGTCTTACCGGTACCGGATGGGCCGACCATCAGCACACCGCGCGGGATACGGCCACCCAGGCGCTGGAACTTGCCCGGATCGCGCAg                                                                >  2:894087/1‑136 (MQ=255)                                                                           aCGGTACTTTCGCTTCGCCCGCGATGGCCTTGGCCAGCAGGGTCTTACCGGTACCGGATGGGCCGACCATCAGCACACCGCGCGGGATACGGCCACCCAGGCGCTGGAACTTGCCCGGATCGCGCAGGAACTCGAc                                                       <  2:754765/136‑1 (MQ=255)                                                                               tACTTTCGCTTCGCCCGCGATGGCCTTGGCCAGCAGGGTCTTACCGGTACCGGATGGGCCGACCATCAGCACACCGCGCGGGATACGGCCACCCAGGCGCTGGAACTTGCCCGGATCGCGCAGGACCTCGACCAGc                                                   >  1:172524/1‑136 (MQ=255)                                                                               tACTTTCGCTTCGCCCGCGATGGCCTTGGCCAGCAGGGTCTTACCGGTACCGGATGGGCCGACCATCAGCACACCGCGCGGGATACGGCCACCCAGGCGCTGGAACTTGCCCGGATCGCGCAGGAACTCGACCAGc                                                   >  1:1019786/1‑136 (MQ=255)                                                                                aCTTTCGCTTCGCCCGCGATGGCCTTGGCCAGCAGGGTCTTACCGGTACCGGATGGGCCGACCATCAGCACACCGCGCGGGATACGGCCACCCAGGCGCTGGAACTTGCCCGGATCGCGCAGGAACTCGACCAGCt                                                  <  1:995011/136‑1 (MQ=255)                                                                                  tttCGCTTCGCCCGCGATGGCCTTGGCCAGCAGGGTCTTACCGGTACCGGATGGGCCGACCATCAGCACACCGCGCGGGATACGGCCACCCAGGCGCTGGAACTTGCCCGGATCGCGCAGGAACTCGACCAGCTCg                                                <  2:296565/136‑1 (MQ=255)                                                                                      gcttcgcCCGCGATGGCCTTGGCCAGCAGGGTCTTACCGGTACCGGATGGGCCGACCATCAGCACACCGCGCGGGATACGGCCACCCAGGCGCTGGAACTTGCCCGGATCGCGCAGGAACTCGACCAGCTCGCCaa                                            <  2:1325454/136‑1 (MQ=255)                                                                                      gcttcgcCCGCGATGGCCTTGGCCAGCAGGGTCTTACCGGTACCGGATGGGCCGACCATCAGCACACCGCGCGGGATACGGCCACCCAGGCGCTGGAACTTGCCCGGATCGCGCAGGAACTCGACCAGCTCGCCaa                                            <  2:212960/136‑1 (MQ=255)                                                                                      gcttcgcCCGCGATGGCCTTGGCCAGCAGGGTCTTACCGGTACCGGATGGGCCGACCATCAGCACACCGCGCGGGATACGGCCACCCAGGCGCTGGAACTTGCCCGGATCGCGCAGGAACTCGACCAGCTCGCCaa                                            <  1:296429/136‑1 (MQ=255)                                                                                            cccGCGATGGCCTTGGCCAGCAGGGTCTTACCGGTACCGGATGGGCCGACCATCAGCACACCGCGCGGGATACGGCCACCCAGGCGCTGGAACTTGCCCGGATCGCGCAGGAACTCGACCAGCTCGCCAACCTCtt                                      >  2:705519/1‑136 (MQ=255)                                                                                                         tGGCCAGCAGGGTCTTACCGGTACCGGATGGGCCGACCATCAGCACACCGCGCGGGATACGGCCACCCAGGCGCTGGAACTTGCCCGGATCGCGCAGGAACTCGACCAGCTCGCCAACCTCTTCCTTGGCCtcgtc                         >  1:499672/1‑136 (MQ=255)                                                                                                             cagcagGGTCTTACCGGTACCGGATGGGCCGACCATCAGCACACCGCGCGGGATACGGCCACCCAGGCGCTGGAACTTGCCCGGATCGCGCAGGAACTCGACCAGCTCGCCACCCTCTTCCTTGGCCTCGTCGCAg                     >  2:731981/1‑136 (MQ=255)                                                                                                             cagcagGGTCTTACCGGTACCGGATGGGCCGACCATCAGCACACCGCGCGGGATACGGCCACCCAGGCGCTGGAACTTGCCCGGATCGCGCAGGAACTCGACCAGCTCGCCAACCTCTTCCTTGGCCTCGTCGCAg                     >  2:1189127/1‑136 (MQ=255)                                                                                                              agcagGGTCTTACCGGTACCGGATGGGCCGACCATCAGCACACCGCGCGGGATACGGCCACCCAGGCGCTGGAACTTGCCCGGATCGCGCAGGAACTCGACCAGCTCGCCAACCTCTTCCTTGGCCTCGTCGCAGc                    >  2:1204765/1‑136 (MQ=255)                                                                                                                   ggTCTTACCGGTACCGGATGGGCCGACCATCAGCACACCGCGCGGGATACGGCCACCCAGGCGCTGGAACTTGCCCGGATCGCGCAGGAACTCGACCAGCTCGCCAACCTCTTCCTTGGCCTCGTCGCAGCCCGCa               <  2:854584/136‑1 (MQ=255)                                                                                                                             gtaccggATGGGCCGACCATCAGCACACCGCGCGGGATACGGCCACCCAGGCGCTGGAACTTGCCCGGATCGCGCAGGAACTCGACCAGCTCGCCAACCTCTTCCTTGGCCTCGTCGCAGCCCGCAACGTcagcca     <  2:499672/136‑1 (MQ=255)                                                                                                                             gtaccggATGGGCCGACCATCAGCACACCGCGCGGGATACGGCCACCCAGGCGCTGGAACTTGCCCGGATCGCGCAGGAACTCGACCAGCTCGCCAACCTCTTCCTTGGCCTCGTCGCAGCCCGCAACGTcagcca     <  2:1349183/136‑1 (MQ=255)                                                                                                                             gtaccggATGGGCCGACCATCAGCACACCGCGCGGGATACGGCCACCCAGGCGCTGGAACTTGCCCGGATCGCGCAGGAACTCGACCAGCTCGCCAACCTCTTCCTTGGCCTCGTCGCAGCCCGCAACGTcagcca     <  1:845735/136‑1 (MQ=255)                                                                                                                             gtaccggATGGGCCGACCATCAGCACACCGCGCGGGATACGGCCACCCAGGCGCTGGAACTTGCCCGGATCGCGCAGGAACTCGACCAGCTCGCCAACCTCTTCCTTGGCCTCGTCGCAGCCCGCAACGTcagcca     <  1:1251218/136‑1 (MQ=255)                                                                                                                             gtaccggATGGGCCGACCATCAGCACACCGCGCGGGATACGGCCACCCAGGCGCTGGAACTTGCCCGGATCGCGCAGGAACTCGACCAGCTCGCCAACCTCTTCCTTGGCCTCGTCGCAGCCCGCAACGTcagcca     <  1:1230187/136‑1 (MQ=255)                                                                                                                             gtaccggATGGGCCGACCATCAGCACACCGCGCGGGATACGGCCACCCAGGCGCTGGAACTTGCCCGGATCGCGCAGGAACTCGACCAGCTCGCCAACCTCTTCCTTGGCCTCGTCGCAGCCCGCAACGTcagcca     <  1:1065683/136‑1 (MQ=255)                                                                                                                                ccggATGGGCCGACCATCAGCACACCGCGCGGGATACGGCCACCCAGGCGCTGGAACTTGCCCGGATCGCGCAGGAACTCGACCAGCTCGCCAACCTCTTCCTTGGCCTCGTCGCAGCCCGCAACGTCAGCCAGCg  >  1:382848/1‑136 (MQ=255)                                                                                                                                    |                                                                                                                                    GAACATGTCGCGCACACGGCTGGCACCGACACCCACGAACATTTCCACGAAGTCCGAACCGGAAATGGTGAAGAACGGTACTTTCGCTTCGCCCGCGATGGCCTTGGCCAGCAGGGTCTTACCGGTACCGGGTGGGCCGACCATCAGCACACCGCGCGGGATACGGCCACCCAGGCGCTGGAACTTGCCCGGATCGCGCAGGAACTCGACCAGCTCGCCAACCTCTTCCTTGGCCTCGTCGCAGCCCGCAACGTCAGCCAGCG  >  NC_002947/5367800‑5368062

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑