Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 5,585,751 C→T A652T (GCG→ACG)  parC ← DNA topoisomerase IV subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,585,7510CT100.0% 150.8 / NA 43A652T (GCG→ACG) parCDNA topoisomerase IV subunit A
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/30);  total (13/30)

CAAATTCTTCACGGCTGGCCACCCGGTCACCCGGCACGCCAATGATCTTGTTGCCCTTGCCTTTACCCAGCTGTGGCAAGTCGCTGACCTTGAACACCAGCAGGCGGCCTTCGGTGGTCACCGCCGCCAACCAGTCCTGCTCGCGATCGGCCACCGGGCGCGGGGTCATGACCTTGGCCCCGTTGGGCAAGCTGAGCAGGCCTTTGCCGGCCTTGTTCTTGGCCTGCAGGTCTTCACCCTTGACCACGAAGCCGTAAC  >  NC_002947/5585625‑5585882
                                                                                                                              |                                                                                                                                   
cAAATTCTTCACGGCTGGCCACCCGGTCACCCGGCACGCCAATGATCTTGTTGCCCTTGCCTTTACCCAGCTGTGGCAAGTCGCTGACCTTGAACACCAGCAGGCGGCCTTCGGTGGTCACCGCCGTCAACCAGTc                                                                                                                            >  2:760071/1‑136 (MQ=255)
     tcttcACGGCTGGCCACCCGGTCACCCGGCACGCCAATGATCTTGTTGCCCTTGCCTTTACCCAGCTGTGGCAAGTCGCTGACCTTGAACACCAGCAGGCGGCCTTCGGTGGTCACCGCCGTCAACCAGTCCTGCt                                                                                                                       >  2:1256087/1‑136 (MQ=255)
        tcACGGCTGGCCACCCGGTCACCCGGCACGCCAATGATCTTGTTGCCCTTGCCTTTACCCAGCTGTGGCAAGTCGCTGACCTTGAACACCAGCAGGCGGCCTTCGGTGGTCACCGCCGTCAACCAGTCCTGCTcgc                                                                                                                    <  1:1071201/136‑1 (MQ=255)
        tcACGGCTGGCCACCCGGTCACCCGGCACGCCAATGATCTTGTTGCCCTTGCCTTTACCCAGCTGTGGCAAGTCGCTGACCTTGAACACCAGCAGGCGGCCTTCGGTGGTCACCGCCGTCAACCAGTCCTGCTcgc                                                                                                                    <  2:453790/136‑1 (MQ=255)
                    aCCCGGTCACCCGGCACGCCAATGATCTTGTTGCCCTTGCCTTTACCCAGCTGTGGCAAGTCGCTGACCTTGAACACCAGCAGGCGGCCTTCGGTCGTCACCGCCGTCAACCAGTCCAGCTCGCGATCGGCCAccc                                                                                                        >  1:1368323/1‑135 (MQ=255)
                     cccGGTCACCCGGCACGCCAATGATCTTGTTGCCCTTGCCTTTACCCAGCTGTGGCAAGTCGCTGACCTTGAACACCAGCAGGCGGCCTTCGGTGGTCACCGCCGTCAACCAGTCCTGCTCGCGATCGGCCACCgg                                                                                                       <  1:1310132/136‑1 (MQ=255)
                      ccGGTCACCCGGCACGCCAATGATCTTGTTGCCCTTGCCTTTACCCAGCTGTGGCAAGTCGCTGACCTTGAACACCAGCAGGCGGCCTTCGGTGGTCACCGCCGTCAACCAGTCCTGCTCGCGATCGGCCACCggg                                                                                                      <  1:967383/136‑1 (MQ=255)
                       cGGTCACCCGGCACGCCAATGATCTTGTTGCCCTTGCCTTTACCCAGCTGTGGCAAGTCGCTGACCTTGAACACCAGCAGGCGGCCTTCGGTGGTCACCGCCGTCAACCAGTCCTGCTCGCGATCGGCCACCGGgc                                                                                                     <  2:1233473/136‑1 (MQ=255)
                       cGGTCACCCGGCACGCCAATGATCTTGTTGCCCTTGCCTTTACCCAGCTGTGGCAAGTCGCTGACCTTGAACACCAGCAGGCGGCCTTCGGTGGTCACCGCCGTCAACCAGTCCTGCTCGCGATCGGCCACCGGgc                                                                                                     <  1:523433/136‑1 (MQ=255)
                       cGGTCACCCGGCACGCCAATGATCTTGTTGCCCTTGCCATTACCCAGCTGTGGCAAGTCGCTGACCTTGAACACCAGCAGGCGGCCTTCGGTGGTCACCGCCGTCAACCAGTCCTGCTCGCGATCGGCCACCGGgc                                                                                                     <  2:1314955/136‑1 (MQ=255)
                       cGGTCACCCGGCACGCCAATGATCTTGTTGCCCTTGACTTTACCCAGCTGTGGCAAGTCGCTGACCTTGAACACCAGCAGGCGGCCTTCGGTGGTCACCGCCGTCAACCAGTCCTGCTCGCGATCGGCCACCGGgc                                                                                                     <  1:1197727/136‑1 (MQ=255)
                            aCCCGGCACGCCAATGATCTTGTTGCCCTTGCCTTTACCCAGCTGTGGCAAGTCGCTGACCTTGAACACCAGCAGGCGGCCTTCGGTGGTCACCGCCGTCAACCAGTCCTGCTCGCGATCGGCCACCGGGCGCggg                                                                                                <  1:760071/136‑1 (MQ=255)
                              ccGGCTCGGCTATGATCTTGTTGCCCTCGCCTTTACCCAGCTGTGGCAAGTCGCTGAGCTTGAACACCAGCAGGCGGCCTTCGGTGGTCACCGGCGTCATCCAGTCCTGCTCGCGATCGGCCACCGGGCGCGGGGt                                                                                              <  2:272968/136‑1 (MQ=255)
                              ccGGCACGCCAATGATCTTGTTGCCCTTGCCTTTACCCAGCTGTGGCAAGTCGCTGACCTTGAACACCAGCAGGCGGCCTTCGGTGGTCACCGCCGTCAACCAGTCCTGCTCGCGATCGGCCACCGGGCGCGGGGt                                                                                              <  1:579676/136‑1 (MQ=255)
                              ccGGCACGCCAATGATCTTGTTGCCCTTGCCTTTACCCAGCTGTGGCAAGTCGCTGACCTTGAACACCAGCAGGCGGCCTTCGGTGGTCACCGCCGTCAACCAGTCCTGCTCGCGATCGGCCACCGGGCGCGGGGt                                                                                              <  1:603243/136‑1 (MQ=255)
                                   aCGCCAATGATCTTGTTGCCCTTGCCTTTACCCAGCTGTGGCAAGTCGCTGACCTTGAACACCAGCAGGCGGCCTTCGGTGGTCACCGCCGTCAACCAGTCCTGCTCGCGATCGGCCACCGGGCGCGGGGTCATGa                                                                                         >  2:318116/1‑136 (MQ=255)
                                     gCCAATGATCTTGTTGCCCTTGCCTTTACCCAGCTGTGGCAAGTCGCTGACCTTGAACACCAGCAGGCGGCCTTCGGTGGTCACCGCCGTCAACCAGTCCTGCTCGCGATCGGCCACCGGGCGCGGGGTCATGAcc                                                                                       >  2:143097/1‑136 (MQ=255)
                                      ccAATGATCTTGTTGCCCTTGCCTTTACCCAGCTGTGGCAAGTCGCTGACCTTGAACACCAGCAGGCGGCCTTCGGTGGTCACCGCCGTCAACCAGTCCTGCTCGCGATCGGCCACCGGGCGCGGGGTCATGACCt                                                                                      <  2:1099738/136‑1 (MQ=255)
                                      ccAATGATCTTGTTGCCCTTGCCTTTACCCAGCTGTGGCAAGTCGCTGACCTTGAACACCAGCAGGCGGCCTTCGGTGGTCACCGCCGTCAACCAGTCCTGCTCGCGATCGGCCACCGGGCGCGGGGTCATGACCt                                                                                      <  1:534653/136‑1 (MQ=255)
                                                  ttgCCCTTGCCTTTACCCAGCTGTGGCAAGTCGCTGACCTTGAACACCAGCAGGCGGCCTTCGGTGGTCACCGCCGTCAACCAGTCCTGCTCGCGATCGGCCACCGGGCGCGGGGTCATGACCTTGGCCCCGTTgg                                                                          <  1:642916/136‑1 (MQ=255)
                                                    gCCCTTGCCTTTACCCAGCTGTGGCAAGTCGCTGACCTTGAACACCAGCAGGCGGCCTTCGGTGGTCACCGCCGTCAACCAGTCCAGCTCGCGATCGGCCACCGGGCGCGGGGTCATGACCTTGGCCCCGTTGGGc                                                                        >  1:744386/1‑136 (MQ=255)
                                                       cTTGCCTTTACCCAGCTGTGGCAAGTCG‑‑GACCTTGAACACCAGCAGGCGGCCTTCGGTGGTCACCGCCGTCAACCAGTCCTGCTCGCGATCGGCCACCGGGCGCGGGGTCATGACCTTGGCCCCGTTGGGCAAGCt                                                                   <  1:600712/136‑1 (MQ=255)
                                                         tGCCTTTACCCAGCTGTGGCAAGTCTCTGACCTTGAACACCAGCAGGCGGCCTTCGGTGGTCACCGCCGTCAACCAGTCCTGCTCGCGATCGGCCACCGGGCGCGGGGTCATGACCTTGGCCCCGTTGGGCAAGCt                                                                   <  1:318116/136‑1 (MQ=255)
                                                         tGCCTTTACCCAGCTGTGGCAAGTCGCTGACCTTGAACACCAGCAGGCGGCCTTCGGTGGTCACCGCCGTCAACCAGTCCTGCTCGCGATCGGCCACCGGGCGCGGGGTCATGACCTTGGCCCCGTTGGGCAAGCt                                                                   <  2:764566/136‑1 (MQ=255)
                                                         tGCCTTTACCCAGCTGTGGCAAGTCGCTGACCTTGAACACCAGCAGGCGGCCTTCGGTGGTCACCGCCGTCAACCAGTCCTGCTCGCGATCGGCCACCGGGCGCGGGGTCATGACCTTGGCCCCGTTGGGCAAGCt                                                                   <  1:908481/136‑1 (MQ=255)
                                                         tGCCTTTACCCAGCTGTGGCAAGTCGCTGACCTTGAACACCAGCAGGCGGCCTTCGGTGGTCACCGCCGTCAACCAGTCCTGCTCGCGATCGGCCACCGGGCGCGGGGTCATGACCTTGGCCCCGTTGGGCAAGCt                                                                   <  1:115095/136‑1 (MQ=255)
                                                         tGCCTTTACCCAGCTGTGGCAAGTCGCTGACCTTGAACACCAGCAGGCGGCCTTCGGTGGTCACCGCCGTCAACCAGTCCTGCTCGCGATCGGCCACCGGGCGCGGGGTCATGACCTTGGCCCCGTTGGGCAAGCt                                                                   <  1:237907/136‑1 (MQ=255)
                                                             tttACCCAGCTGTGGCAAGTCGCTGACCTTGAACACCAGCAGGCGGCCTTCGGTGGTCACCGCCGTCAACCAGTCCTGCTCGCGATCGGCCACCGGGCGCGGGGTCATGACCTTGGCCCCGTTGGGCAAGCTGAGc                                                               >  1:672223/1‑136 (MQ=255)
                                                                   cAGCTGTGGCAAGTCGCTGACCTTGAACACCAGCAGGCGGCCTTCGGTGGTCACCGCCGTCAACCAGTCCTGCTCGCGATCGGCCACCGGGCGCGGGGTCATGACCATGGCCCCGTTGGGCAAGCTGAGCAGGCCt                                                         >  1:303177/1‑136 (MQ=255)
                                                                    aGCTGTGGCAAGTCGCTGACCTTGAACACCAGCAGGCGGCCTTCGGTGGTCACCGCCGTCAACCAGTCCTGCTCGCGATCGGCCACCGGGCGCGGGGTCATGACCTTGGCCCCGTTGGGCAAGCTGAGCAGGCCtt                                                        <  1:276953/136‑1 (MQ=255)
                                                                         tgGCAAGTCGCTGACCTTGAACACCAGCAGGCGGCCTTCGGTGGTCACCGCCGTCAACCAGTCCTGCTCGCGATCGGCCACCGGGCGCGGGGTCATGACCTTTGCCCCGTTGGGCAAGCAGAGCAGGCCTTTGCCg                                                   >  2:1105310/1‑136 (MQ=255)
                                                                                  gCTGACCTTGAACACCAGCAGGCGGCCTTCGGTGGTCACCGCCGTCAACCAGTCCTGCTCGCGATCGGCCACCGGGCGCGGGGTCATGACCTTGGCCCCGTTGGGCAAGCTGAGCAGGCCTTTGCCGGCCTTGTTc                                          <  2:987688/136‑1 (MQ=255)
                                                                                  gCTGACCTTGAACACCAGCAGGCGGCCTTCGGTGGTCACCGCCGTCAACCAGTCCTGCTCGCGATCGGCCACCGGGCGCGGGGTCATGACCTTGGCCCCGTTGGGCAAGCTGAGCAGGCCTTTGCCGGCCTTGTTc                                          <  2:306964/136‑1 (MQ=255)
                                                                                      aCCTTGAACACCAGCAGGCGGCCTTCGGTGGTCACCGCCGTCAACCAGTCCTGCTCGCGATCGGCCACCGGGCGCGGGGTCATGACCTTGGCCCCGTTGGGCAAGCTGAGCAGGCCTTTGCCGGCCTTGTTCTTgg                                      >  2:311055/1‑136 (MQ=255)
                                                                                        cTTGAACACCAGCAGGGGGCCTTCTGTGGTCACCGCCGTCAACCAGTGCTGCTCGCGATCGGCCACCGGGCGCGGGGTCATGACCTTGGCCCCGTTGGGCTAGCTGAGCAGGCCTTTGCCGGCCTTGTTCTTGGcc                                    <  1:383645/136‑1 (MQ=255)
                                                                                        cTTGAACACCAGCAGGCGGCCTTCGGTGGTCACCGCCGTCAACCAGTCCTGCTCGCGATCGGCCACCGGGCGCGGGGTCATGACCTTGGCCCCGTTGGGCAAGCTGAGCAGGCCTTTGCCGGCCTTGTTCTTGGcc                                    <  1:562237/136‑1 (MQ=255)
                                                                                        cTTGAACACCAGCAGGCGGCCTTCGGTGGTCACCGCCGTCAACCAGTCCTGCTCGCGATCGGCCACCGGGCGCGGGGTCATGACCTTGGCCCCGTTGGGCAAGCTGAGCAGGCCTTTGCCGGCCTTGTTCTTGGcc                                    <  1:1344704/136‑1 (MQ=255)
                                                                                        cTTGAACACCAGCAGGCGGCCTTCGGTGGTCACCGCCGTCAACCAGTCCTGCTCGCGATCGGCCACCGGGCGCGGGGTCATGACCTTGGCCCCGTTGGGCAAGCTGAGCAGGCCTTTGCCGGCCTTGTTCTTGGcc                                    <  1:1293042/136‑1 (MQ=255)
                                                                                        cTTGAACACCAGCAGGCGGCCTTCGGTGGTCACCGCCGTCAACCAGTCCTGCTCGCGATCGGCCACCGGGCGCGGGGCCATGACCTTGGCCCCGTTGGGCAAGCTGAGCAGGCCTTTGCCGGCCTTGTTCTTGGcc                                    <  1:1093485/136‑1 (MQ=255)
                                                                                                    cagGCGGCCTTCGGTGGTCACCGCCGTCAACCAGTCCTGCTCGCGATCGGCCACCGGGCGCGGGGTCATGACCTTGGCCCCGTTGGGCAAGCTGAGCAGGCCTTTGCCGGCCTTGTTCTTGGCCTGCAGGTCTTcc                        >  1:788763/1‑135 (MQ=255)
                                                                                                      ggcggcCTTCGGTGGTCACCGCCGTCAACCAGTCCTGCTCGCGATCGGCCACCGGGCGCGGGGTCATGACCTTGGCCCCGTTGGGCAAGCTGAGCAGGCCTTTGCCGGCCTTGTTCTTGGCCTGCAGGTCTTCAcc                      <  1:143097/136‑1 (MQ=255)
                                                                                                                 gtggtCACCGCCGTCAACCAGTCCTGCTCGCGATCGGCCACCGGGCGCGGGGTCATGACCTTGGCCCCGTTGGGCAAGCTGAGCAGGCCTTTGCCGGCCTTGTTCTTGGCCTGCAGGTCTTCACCCTTGACCACGa           >  1:1341611/1‑136 (MQ=255)
                                                                                                                        ccgccgTCAACCAGTCCTGCTCGCGATCGGCCACCGGGCGCGGGGTCATGACCTTGGCCCCGTTGGGCAAGCTGAGCAGGCCTTTGCCGGCCTTGTTCTTGGCCTGCAGGTCTTCACCCTTGACCACGAAGCCGTa    >  1:1231601/1‑136 (MQ=255)
                                                                                                                          cccggcAACCAGTCCTGCTCGCGATCGGCCACCGGGCGCGGGGTCATGACCTTGGCCCCGTTGGGCAAGCTGAGCAGGCCTTTGCCGGCCTTGTTCTTGGCCTGCAGGTCTTCACCCTTGACCACGAAGCCGTAAc  <  1:839748/135‑1 (MQ=255)
                                                                                                                              |                                                                                                                                   
CAAATTCTTCACGGCTGGCCACCCGGTCACCCGGCACGCCAATGATCTTGTTGCCCTTGCCTTTACCCAGCTGTGGCAAGTCGCTGACCTTGAACACCAGCAGGCGGCCTTCGGTGGTCACCGCCGCCAACCAGTCCTGCTCGCGATCGGCCACCGGGCGCGGGGTCATGACCTTGGCCCCGTTGGGCAAGCTGAGCAGGCCTTTGCCGGCCTTGTTCTTGGCCTGCAGGTCTTCACCCTTGACCACGAAGCCGTAAC  >  NC_002947/5585625‑5585882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑