New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_bme_pgi	1613266 =	48 (0.990)	13 (0.270)	12/254	3.8	22.6%	intergenic (‑15/+49)	glsB/sad	glutaminase 2/succinate semialdehyde dehydrogenase, NAD(P)+‑dependent
	?	NC_000913_3_bme_pgi	1613290 =	41 (0.850)					intergenic (‑39/+25)	glsB/sad	glutaminase 2/succinate semialdehyde dehydrogenase, NAD(P)+‑dependent
Rejected: Coverage evenness skew score above cutoff.

CAATCCCCAATCGGGAACCGTCTACTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCACGACCAAAGCCACTCTTTTTCACGCCACCAAAGG  >  NC_000913_3_bme_pgi/1613122‑1613415                                                                                                                                                 |                                                                                                                                                      cAATCCCCAATCGGGAACCGTCTACTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAg                                                                                                                                                  >  3:154396/1‑150 (MQ=255) cAATCCCCAATCGGGAACCGTCTACTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAg                                                                                                                                                  <  2:190955/150‑1 (MQ=255)  aaTCCCCAATCGGGAACCGTCTACTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAgg                                                                                                                                                 <  4:154396/150‑1 (MQ=255)      cccAATCGGGAACCGTCTACTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCCTGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTg                                                                                                                                             >  3:70115/1‑150 (MQ=255)      cccAATCGGGAACCGTCTACTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTg                                                                                                                                             >  2:61044/1‑150 (MQ=255)            cGGGAACCGTCTACTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGa                                                                                                                                       <  2:379034/150‑1 (MQ=255)                 aCCGTCTACTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGc                                                                                                                                  <  1:61044/150‑1 (MQ=255)                 aCCGTCTACTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGc                                                                                                                                  <  1:507360/150‑1 (MQ=255)                 aCCGTCTACTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGc                                                                                                                                  <  3:96514/150‑1 (MQ=255)                   cGTCTACTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGaaaa                                                                                                                                         >  1:43594/1‑141 (MQ=255)                   cGTCTACTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGaaaa                                                                                                                                         <  2:43594/141‑1 (MQ=255)                     tCTACTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGt                                                                                                                              <  3:158281/150‑1 (MQ=255)                      cTACTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTTTGAAAAGACCTGCGAGta                                                                                                                             <  2:385288/150‑1 (MQ=255)                       tACTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGtat                                                                                                                            >  4:327829/1‑150 (MQ=255)                             aGCCAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCaga                                                                                                                      <  3:237283/150‑1 (MQ=255)                                cAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAg                                                                                                                                        >  3:182792/1‑129 (MQ=255)                                cAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAg                                                                                                                                        <  4:182792/129‑1 (MQ=255)                                   cgcCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGaa                                                                                                                <  2:376747/150‑1 (MQ=255)                                          atatAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAACGACCTGCGAGTATATCAGAGCTGAATATGTcg                                                                                                         <  2:195016/150‑1 (MQ=255)                                           tataATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTcgc                                                                                                        >  3:35393/1‑150 (MQ=255)                                              aaTCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTc                                                                                                     >  2:296041/1‑150 (MQ=255)                                              aaTCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTc                                                                                                     >  2:147667/1‑150 (MQ=255)                                               aTCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCa                                                                                                    >  2:237883/1‑150 (MQ=255)                                                  cgcgACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGAt                                                                                                 >  2:394085/1‑150 (MQ=255)                                                     gACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCg                                                                                              >  3:256455/1‑150 (MQ=255)                                                      aCTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCgg                                                                                             <  1:394085/150‑1 (MQ=255)                                                          tACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGc                                                                                                                                  <  2:34511/109‑1 (MQ=255)                                                          tACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGc                                                                                                                                  >  1:34511/1‑109 (MQ=255)                                                              cTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCca                                                                                     <  1:147667/150‑1 (MQ=255)                                                                   cAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCg                                                                                <  1:237883/150‑1 (MQ=255)                                                                     aTGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTc                                                                              >  4:288039/1‑150 (MQ=255)                                                                                      ccGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTc                                                             <  1:296041/150‑1 (MQ=255)                                                                                                   tCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAg                                                                                                                                        <  1:188157/62‑1 (MQ=255)                                                                                                   tCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAg                                                                                                                                        >  2:188157/1‑62 (MQ=255)                                                                                                     tAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTg                                                                                                                                   <  2:255306/65‑1 (MQ=255)                                                                                                     tAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTg                                                                                                                                   >  1:255306/1‑65 (MQ=255)                                                                                                              aTTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTc                                     <  3:327829/150‑1 (MQ=255)                                                                                                                   ccATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTg                                                                                                                  <  2:207727/68‑1 (MQ=255)                                                                                                                   ccATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTg                                                                                                                  >  1:207727/1‑68 (MQ=255)                                                                                                                     aTGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCACGACCa                              >  1:477471/1‑150 (MQ=255)                                                                                                                      tGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCACGAcc                               >  2:428502/1‑148 (MQ=255)                                                                                                                      tGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCACGAcc                               <  1:428502/148‑1 (MQ=255)                                                                                                                         cGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCACGACCAAAGc                          <  4:35393/150‑1 (MQ=255)                                                                                                                           aCTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCACGACCAAAGCCa                        <  3:288039/150‑1 (MQ=255)                                                                                                                             tGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCACGACCAAAGCCAct                      >  3:281116/1‑150 (MQ=255)                                                                                                                                   tttCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCgt                                                           <  1:98726/107‑1 (MQ=255)                                                                                                                                   tttCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCgt                                                           >  2:98726/1‑107 (MQ=255)                                                                                                                                          tCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCACGACCAAAGCCACTCTTTTTCACGcca         >  4:151295/1‑150 (MQ=255)                                                                                                                                                 ccGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGa                                                                 >  3:42188/1‑87 (MQ=255)                                                                                                                                                 ccGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGa                                                                 <  4:42188/87‑1 (MQ=255)                                                                                                                                                 ccGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCACGACCAAAGCCACTCTTTTTCACGCCACCAAAgg  >  1:293377/1‑150 (MQ=255)                                                                                                                                                 |                                                                                                                                                      CAATCCCCAATCGGGAACCGTCTACTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCACGACCAAAGCCACTCTTTTTCACGCCACCAAAGG  >  NC_000913_3_bme_pgi/1613122‑1613415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.