Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_bme_pgi2,637,4390CA21.1% 134.3 / 24.8 52intergenic (‑83/+35)der/bamBGTPase; multicopy suppressor of ftsJ/BamABCDE complex OM biogenesis lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (23/18);  new base A (5/6);  total (28/24)
Fisher's exact test for biased strand distribution p-value = 7.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TTAGACGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGAACTATCAACTTTTTGCTG  >  NC_000913_3_bme_pgi/2637290‑2637578
                                                                                                                                                     |                                                                                                                                           
ttAGACGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTc                                                                                                                                             >  1:146556/1‑150 (MQ=255)
      ggTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAg                                                                                                                                       >  2:352800/1‑150 (MQ=255)
          aaaTAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCTCCTGGACAGGGGCCGt                                                                                                                                   <  2:18533/150‑1 (MQ=255)
           aaTAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGtt                                                                                                                                  >  1:267684/1‑150 (MQ=255)
           aaTAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGtt                                                                                                                                  >  4:269436/1‑150 (MQ=255)
           aaTAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGATAAAAACAGAAAAACGGCTCCTGGACAGGGGCCGtt                                                                                                                                  <  2:568509/150‑1 (MQ=255)
             tAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGtttt                                                                                                                                <  2:146556/150‑1 (MQ=255)
                 gTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCaaa                                                                                                                            >  3:37964/1‑150 (MQ=255)
                  tGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAg                                                                                                                           >  4:216484/1‑150 (MQ=255)
                                ttAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGAc                                                                                                             <  1:468920/150‑1 (MQ=255)
                                    ggCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGAtt                                                                                                         >  2:248532/1‑150 (MQ=255)
                                       gcCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAAc                                                                                                      <  4:80403/150‑1 (MQ=255)
                                        cccGCCAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTGTAAATCAGCAGACAACGCACGCTATATTCTCGTCGTTGTTTAAAACATGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACg                                                                                                     >  2:150890/1‑150 (MQ=255)
                                         ccGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACgt                                                                                                    >  4:189408/1‑150 (MQ=255)
                                           gACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTGAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACgtgt                                                                                                  >  2:557992/1‑150 (MQ=255)
                                            aCAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCaaa                                                                                                                            >  1:54701/1‑123 (MQ=255)
                                            aCAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCaaa                                                                                                                            <  2:54701/123‑1 (MQ=255)
                                              aaGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAAt                                                                                               >  3:7721/1‑150 (MQ=255)
                                                 cgcgACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATaga                                                                                            <  3:269436/150‑1 (MQ=255)
                                                        acaGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCTCCTGGACAGGGGCCGt                                                                                                                                   >  2:384775/1‑104 (MQ=38)
                                                        acaGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCTCCTGGACAGGGGCCGt                                                                                                                                   <  1:384775/104‑1 (MQ=38)
                                                               ccATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGt                                                                              >  4:103692/1‑150 (MQ=255)
                                                                cATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTc                                                                             <  3:189408/150‑1 (MQ=255)
                                                                       aaaGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGcc                                                                      <  1:248532/150‑1 (MQ=255)
                                                                        aaGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGa                                                                                                           <  1:93687/112‑1 (MQ=255)
                                                                        aaGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGa                                                                                                           >  2:93687/1‑112 (MQ=255)
                                                                           ccTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGt                                                                              <  2:472299/138‑1 (MQ=255)
                                                                           ccTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGt                                                                              >  1:472299/1‑138 (MQ=255)
                                                                                 ttttATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATcagca                                                            <  4:37964/150‑1 (MQ=255)
                                                                                  tttATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACgtg                                                                                                   <  4:219279/110‑1 (MQ=255)
                                                                                  tttATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACgtg                                                                                                   >  3:219279/1‑110 (MQ=255)
                                                                                      taAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTg                                                       >  2:296987/1‑150 (MQ=255)
                                                                                       aaaTCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGc                                                      <  1:508476/150‑1 (MQ=255)
                                                                                         atcatcAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCaaa                                                                                                                            >  2:254744/1‑78 (MQ=255)
                                                                                         atcatcAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCaaa                                                                                                                            <  1:254744/78‑1 (MQ=255)
                                                                                                gACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCgg                                             >  1:274615/1‑150 (MQ=255)
                                                                                                         cgcTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGtt                                    <  1:557992/150‑1 (MQ=255)
                                                                                                             atatTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCCGTTTGCCGTCAGCGGCAACCGGTTCAGt                                <  2:267684/150‑1 (MQ=255)
                                                                                                                ttCGCGTCGTTGTTAAAAGCAGGATACCGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCGGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAgacgg                             <  2:183951/150‑5 (MQ=255)
                                                                                                                     gtcgtTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGCTCAGTCTGGAAAc                        >  2:510767/1‑150 (MQ=255)
                                                                                                                      tcgtTGTTAAAAACAGGAAAACGGCCCCGGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAAcc                       >  4:105093/1‑150 (MQ=255)
                                                                                                                            ttAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGAACt                 <  2:384781/150‑1 (MQ=255)
                                                                                                                                  cttttaaaaCGGCTCCTGGACAGAGGCCGCTTTCAAAGTGAACGCCAGAGACGCTTAACGTGTAATAGAGTACACGGGTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGAACGATGAAc           >  2:565728/6‑150 (MQ=255)
                                                                                                                                   tttgAAAACGGCTCCTGGACAGGGGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCt                                                                     <  2:570604/88‑1 (MQ=25)
                                                                                                                                   tttgAAAACGGCTCCTGGACAGGGGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCt                                                                     >  1:570604/4‑91 (MQ=25)
                                                                                                                                     ggAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGAACTATCAACttt        >  3:292100/1‑150 (MQ=255)
                                                                                                                                        aaaCGGCTCCTGGACAGGGGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTcc                                                                                >  2:120210/1‑75 (MQ=25)
                                                                                                                                        aaaCGGCTCCTGGACAGGGGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTcc                                                                                <  1:120210/75‑1 (MQ=25)
                                                                                                                                        aaaCGGCTCCTGGACAGGGGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGaa                   <  1:385094/136‑1 (MQ=255)
                                                                                                                                        aaaCGGCTCCTGGACAGGGGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTCCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGaa                   >  2:385094/1‑136 (MQ=255)
                                                                                                                                        aaaCGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGAACTATCAACTTTTTg     >  2:293751/1‑150 (MQ=255)
                                                                                                                                         aaCGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGAACTATCAACTTTTTGc    >  2:242138/1‑150 (MQ=255)
                                                                                                                                           cGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGAACTATCAACTTTTTGCTg  <  4:7721/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                           
TTAGACGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGAACTATCAACTTTTTGCTG  >  NC_000913_3_bme_pgi/2637290‑2637578

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑