Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_bme_pgi 4,233,689 C→T intergenic (‑456/‑2218) lysC ← / → yjbE lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_bme_pgi4,233,6890CT100.0% 444.8 / NA 122intergenic (‑456/‑2218)lysC/yjbElysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (59/63);  total (59/63)

Only 100 of 123 total aligned reads displayed.

TATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGAT  >  NC_000913_3_bme_pgi/4233543‑4233835
                                                                                                                                                  |                                                                                                                                                  
tataAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATtaca                                                                                                                                                 <  2:265573/150‑1 (MQ=255)
   aaataaaGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTACGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACAttt                                                                                                                                              >  3:826953/1‑150 (MQ=255)
   aaataaaGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACAttt                                                                                                                                              >  4:381215/1‑150 (MQ=255)
      taaaGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTc                                                                                                                                           >  4:58770/1‑150 (MQ=255)
        aaGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCtg                                                                                                                                         <  2:1355584/150‑1 (MQ=255)
         atggTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCtgt                                                                                                                                        <  1:1521988/148‑1 (MQ=255)
             tGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACt                                                                                                                                    >  4:849617/1‑150 (MQ=255)
             tGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACt                                                                                                                                    >  1:396440/1‑150 (MQ=255)
              gAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTg                                                                                                                                   <  1:440506/150‑1 (MQ=255)
                 cGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGgcg                                                                                                                                <  1:747313/150‑1 (MQ=255)
                     gCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGGTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACCAGAACCATTACATTTGTCTGTGACTGGCGCTAc                                                                                                                            >  4:584514/1‑150 (MQ=255)
                             cAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTcc                                                                                                                    >  3:427237/1‑150 (MQ=255)
                              aaCGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCa                                                                                                                   <  2:83732/150‑1 (MQ=255)
                                           tGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATtct                                                                                                      <  1:1574643/150‑1 (MQ=255)
                                            gCGGATTTTTCATCCCCTCCGGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATtctc                                                                                                     <  3:152017/150‑1 (MQ=255)
                                            gCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCaaa                                                                                                                 <  1:1361698/138‑1 (MQ=255)
                                            gCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCaaa                                                                                                                 >  2:1361698/1‑138 (MQ=255)
                                             cGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACAttttt                                                                                                                                            <  4:439601/110‑1 (MQ=255)
                                             cGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACAttttt                                                                                                                                            >  3:439601/1‑110 (MQ=255)
                                             cGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCa                                                                                                    >  3:130094/1‑150 (MQ=255)
                                              ggATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCaa                                                                                                   <  2:219273/150‑1 (MQ=255)
                                               gATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCaaa                                                                                                  <  3:976474/150‑1 (MQ=255)
                                                   tttCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCa                                                                                              <  2:1067129/150‑1 (MQ=255)
                                                           aCTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTca                                                                                                             <  4:48034/127‑1 (MQ=255)
                                                           aCTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTa                                                                                      >  4:625175/1‑150 (MQ=255)
                                                              ccTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGACGAGTATTg                                                                                   <  2:324717/150‑1 (MQ=255)
                                                               cTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATtct                                                                                                      <  3:778691/130‑1 (MQ=255)
                                                               cTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATtct                                                                                                      >  4:778691/1‑130 (MQ=255)
                                                               cTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGc                                                                                  >  2:807221/1‑150 (MQ=255)
                                                                tGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCt                                                                                 >  4:75581/1‑150 (MQ=255)
                                                                tGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCt                                                                                 <  1:1362374/150‑1 (MQ=255)
                                                                 ggCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTa                                                                                >  4:651634/1‑150 (MQ=255)
                                                                   cGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAAt                                                                              <  3:625175/150‑1 (MQ=255)
                                                                    ggTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATg                                                                             <  2:93681/150‑1 (MQ=255)
                                                                        agtagtTCAGCTAATAAATACTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATggaaa                                                                         >  2:710715/1‑147 (MQ=255)
                                                                         gtagtTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGgcgc                                                                                                                               <  1:104109/95‑1 (MQ=255)
                                                                         gtagtTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGgcgc                                                                                                                               >  2:104109/1‑95 (MQ=255)
                                                                         gtagtTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAag                                                                        >  3:578586/1‑150 (MQ=255)
                                                                         gtagtTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAag                                                                        >  1:482527/1‑150 (MQ=255)
                                                                            gtTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAgaga                                                                     >  2:456583/1‑150 (MQ=255)
                                                                                aGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTGATGGAAAGAGAcgct                                                                 <  2:182755/150‑2 (MQ=255)
                                                                                aGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGAcgct                                                                 >  4:577791/1‑149 (MQ=255)
                                                                                 gCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCagaa                                                                                           >  3:388353/1‑123 (MQ=255)
                                                                                 gCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCagaa                                                                                           <  4:388353/123‑1 (MQ=255)
                                                                                  cTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGAcgctac                                                               >  4:383068/1‑147 (MQ=255)
                                                                                   taataaATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGAcgct                                                                 >  3:600658/1‑146 (MQ=255)
                                                                                   taataaATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGAcgct                                                                 <  4:600658/147‑2 (MQ=255)
                                                                                     ataaATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGaa                                                            >  2:1295877/1‑150 (MQ=255)
                                                                                         aTGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCtt                                                        <  3:431688/150‑1 (MQ=255)
                                                                                         aTGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCtt                                                        <  4:130094/150‑1 (MQ=255)
                                                                                         aTGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCtt                                                        >  4:431688/1‑150 (MQ=255)
                                                                                           gCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGa                                                      >  3:289970/1‑150 (MQ=255)
                                                                                            cTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGaa                                                     <  3:508757/150‑1 (MQ=255)
                                                                                            cTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGaa                                                     <  1:807221/150‑1 (MQ=255)
                                                                                             ttCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAagag                                                                      <  1:1460206/132‑1 (MQ=255)
                                                                                             ttCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAagag                                                                      >  2:1460206/1‑132 (MQ=255)
                                                                                                 cTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAAc                                                <  1:783566/150‑1 (MQ=255)
                                                                                                  tGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACg                                               >  1:57217/1‑150 (MQ=255)
                                                                                                   gcgcTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACgg                                              >  4:605271/1‑150 (MQ=255)
                                                                                                   gcgcTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACgg                                              <  3:605271/150‑1 (MQ=255)
                                                                                                     gcTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGt                                                                                       >  4:565293/1‑107 (MQ=255)
                                                                                                     gcTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGt                                                                                       <  3:565293/107‑1 (MQ=255)
                                                                                                     gcTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAACGGATAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTc                                            >  3:17100/1‑150 (MQ=255)
                                                                                                       tAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCt                                                                                                                                          >  2:922709/1‑54 (MQ=255)
                                                                                                       tAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCt                                                                                                                                          <  1:922709/54‑1 (MQ=255)
                                                                                                       tAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGc                                          <  3:577791/150‑1 (MQ=255)
                                                                                                          gggTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAGGCAACGGTCGCaaa                                       <  4:427237/150‑1 (MQ=255)
                                                                                                           ggTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATtct                                                                                                      <  3:688064/86‑1 (MQ=255)
                                                                                                            gTTTACAATCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGc                                     <  4:565597/150‑1 (MQ=255)
                                                                                                             tttACCCTCCACAGTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGGCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAGGAGACGCTACGAAGCTTGAAGCAACTGTCGCAAAGCt                                    <  4:826953/150‑1 (MQ=255)
                                                                                                             tttACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGTCGCTACGAAGCTTGAAGCAACGGTAGCAAAGCt                                    <  2:482527/150‑1 (MQ=255)
                                                                                                             tttACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCt                                    <  3:381215/150‑1 (MQ=255)
                                                                                                                acacTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGaa                                 <  4:426300/150‑1 (MQ=255)
                                                                                                                   cTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGaa                                                     >  2:37180/1‑127 (MQ=255)
                                                                                                                   cTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGaa                                                     <  1:37180/127‑1 (MQ=255)
                                                                                                                   cTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGaaaaa                              >  2:852844/1‑150 (MQ=255)
                                                                                                                    tataCATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAg                             <  3:479619/147‑1 (MQ=255)
                                                                                                                    tCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGAcgc                                                                  >  1:515608/1‑113 (MQ=255)
                                                                                                                    tCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGAcgc                                                                  <  2:515608/113‑1 (MQ=255)
                                                                                                                      aaCATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAAtt                                                                                                        <  1:1578634/73‑1 (MQ=255)
                                                                                                                        cATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATtctc                                                                                                     >  1:72029/1‑74 (MQ=255)
                                                                                                                        cATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATtctc                                                                                                     <  2:72029/74‑1 (MQ=255)
                                                                                                                         aTTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGGATAGTTATATTAAAAATACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTg                        >  1:1265724/1‑150 (MQ=255)
                                                                                                                          ttACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTgg                       >  2:1043273/1‑150 (MQ=255)
                                                                                                                           tACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTggg                      <  2:546035/150‑1 (MQ=255)
                                                                                                                              gCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCgg                   <  3:532860/150‑1 (MQ=255)
                                                                                                                                tAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGaa                 <  1:456583/150‑1 (MQ=255)
                                                                                                                                 aaCGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGTGGAAt                >  3:37052/1‑150 (MQ=255)
                                                                                                                                 aaCGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAAt                >  1:503310/1‑150 (MQ=255)
                                                                                                                                  aCGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATc               >  3:766694/1‑150 (MQ=255)
                                                                                                                                  aCGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATc               >  1:1297783/1‑150 (MQ=255)
                                                                                                                                   cGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCg              >  3:249734/1‑150 (MQ=255)
                                                                                                                                     gCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCaaa                                                                                                                 <  4:277374/49‑1 (MQ=255)
                                                                                                                                         tAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCg              >  2:678896/1‑144 (MQ=255)
                                                                                                                                         tAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCg              <  1:678896/144‑1 (MQ=255)
                                                                                                                                            aaCCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCcgcgc     <  4:249734/150‑1 (MQ=255)
                                                                                                                                              ccATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGa   <  1:926250/150‑1 (MQ=255)
                                                                                                                                              ccATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGa   <  4:17100/150‑1 (MQ=255)
                                                                                                                                               cATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGat  >  3:117171/1‑150 (MQ=255)
                                                                                                                                               cATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGat  <  3:75581/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
TATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGAT  >  NC_000913_3_bme_pgi/4233543‑4233835

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: