Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_bme_pgi 4,233,762 C→A intergenic (‑529/‑2145) lysC ← / → yjbE lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_bme_pgi4,233,7620CA100.0% 467.2 / NA 128intergenic (‑529/‑2145)lysC/yjbElysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (67/61);  total (67/61)

Only 100 of 128 total aligned reads displayed.

GTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCG  >  NC_000913_3_bme_pgi/4233616‑4233908
                                                                                                                                                  |                                                                                                                                                   
gtagtTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAag                                                                                                                                                  >  1:482527/1‑150 (MQ=255)
gtagtTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAag                                                                                                                                                  >  3:578586/1‑150 (MQ=255)
   gtTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAgaga                                                                                                                                               >  2:456583/1‑150 (MQ=255)
       aGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGAcgct                                                                                                                                           >  4:577791/1‑149 (MQ=255)
       aGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACAATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGAcgct                                                                                                                                           >  3:731858/1‑149 (MQ=255)
         cTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGAcgctac                                                                                                                                         >  4:383068/1‑147 (MQ=255)
          taataaATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGAcgct                                                                                                                                           <  4:600658/147‑2 (MQ=255)
          taataaATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGAcgct                                                                                                                                           >  3:600658/1‑146 (MQ=255)
            ataaATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGaa                                                                                                                                      >  2:1295877/1‑150 (MQ=255)
                aTGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCtt                                                                                                                                  <  3:431688/150‑1 (MQ=255)
                  gCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGa                                                                                                                                >  3:289970/1‑150 (MQ=255)
                   cTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGaa                                                                                                                               <  3:508757/150‑1 (MQ=255)
                   cTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGaa                                                                                                                               <  1:807554/150‑1 (MQ=255)
                    ttCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAagag                                                                                                                                                >  2:1460206/1‑132 (MQ=255)
                        cTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAAc                                                                                                                          <  1:783566/150‑1 (MQ=255)
                         tGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACg                                                                                                                         >  1:57217/1‑150 (MQ=255)
                          gcgcTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACgg                                                                                                                        >  4:605271/1‑150 (MQ=255)
                            gcTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAACGGATAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTc                                                                                                                      >  3:17100/1‑150 (MQ=255)
                             cTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCg                                                                                                                     >  1:1402722/1‑150 (MQ=255)
                                 gggTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAGGCAACGGTCGCaaa                                                                                                                 <  4:427237/150‑1 (MQ=255)
                                 gggTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCaaa                                                                                                                 <  4:427143/150‑1 (MQ=255)
                                   gTTTACAATCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGc                                                                                                               <  4:565597/150‑1 (MQ=255)
                                    tttACCCTCCACAGTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGGCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAGGAGACGCTACGAAGCTTGAAGCAACTGTCGCAAAGCt                                                                                                              <  4:826953/150‑1 (MQ=255)
                                    tttACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGTCGCTACGAAGCTTGAAGCAACGGTAGCAAAGCt                                                                                                              <  2:482527/150‑1 (MQ=255)
                                       acacTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGaa                                                                                                           <  4:426300/150‑1 (MQ=255)
                                          cTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGaa                                                                                                                               <  1:37180/127‑1 (MQ=255)
                                          cTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGaaaaa                                                                                                        >  2:852844/1‑150 (MQ=255)
                                           tataCATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAg                                                                                                       <  3:479619/147‑1 (MQ=255)
                                           tCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGAcgc                                                                                                                                            >  1:515608/1‑113 (MQ=255)
                                           tCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGAcgc                                                                                                                                            <  2:515608/113‑1 (MQ=255)
                                                aTTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTg                                                                                                  >  1:491194/1‑150 (MQ=255)
                                                aTTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGGATAGTTATATTAAAAATACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTg                                                                                                  >  1:1265724/1‑150 (MQ=255)
                                                     gCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCgg                                                                                             <  3:532860/150‑1 (MQ=255)
                                                       tAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGaa                                                                                           <  1:456583/150‑1 (MQ=255)
                                                        aaCGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGTGGAAt                                                                                          >  3:37052/1‑150 (MQ=255)
                                                          cGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCg                                                                                        >  3:249734/1‑150 (MQ=255)
                                                           ggCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCgg                                                                                       >  3:100311/1‑150 (MQ=255)
                                                                tAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCg                                                                                        <  1:678896/144‑1 (MQ=255)
                                                                   aaCCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCcgcgc                                                                               <  4:249734/150‑1 (MQ=255)
                                                                     ccATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGa                                                                            <  1:926250/150‑1 (MQ=255)
                                                                     ccATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGa                                                                            <  4:17100/150‑1 (MQ=255)
                                                                      cATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGat                                                                           >  3:117171/1‑150 (MQ=255)
                                                                      cATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGat                                                                           <  3:75581/150‑1 (MQ=255)
                                                                       attacaTTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGata                                                                          <  4:117171/147‑1 (MQ=255)
                                                                        ttacaTTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGatat                                                                         >  4:411658/3‑150 (MQ=255)
                                                                         tacaTTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGAcg                                                                                                                                             >  3:387888/2‑82 (MQ=255)
                                                                            aTTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGaa                                                                                           >  2:447412/1‑129 (MQ=255)
                                                                             tttttCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATgcgcg                                                                    <  4:551826/150‑1 (MQ=255)
                                                                              ttttCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATgcgcgc                                                                   <  2:1402722/150‑1 (MQ=255)
                                                                               tttCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGcgcgcg                                                                  <  3:411658/150‑1 (MQ=255)
                                                                                   tgtgACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCAt                                                              >  3:260631/1‑150 (MQ=255)
                                                                                     tgACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCAtt                                                                                                   >  2:532050/1‑112 (MQ=255)
                                                                                     tgACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCAtt                                                                                                   <  1:532050/112‑1 (MQ=255)
                                                                                                aCAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGAccc                                                 >  3:351116/1‑150 (MQ=255)
                                                                                                  aaTCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCcgcgc                                                                               <  2:785744/119‑1 (MQ=255)
                                                                                                  aaTCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCcgcgc                                                                               >  1:785744/1‑119 (MQ=255)
                                                                                                    tCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCccggc                                             >  3:585746/1‑150 (MQ=255)
                                                                                                    tCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCccggc                                             >  2:591296/1‑150 (MQ=255)
                                                                                                    tCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCccggc                                             <  3:917428/150‑1 (MQ=255)
                                                                                                      ttCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGTGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCccggccg                                           >  1:1371726/1‑150 (MQ=255)
                                                                                                         cAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGtt                                        <  2:57217/150‑1 (MQ=255)
                                                                                                          aaaGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTc                                       >  2:203843/1‑150 (MQ=255)
                                                                                                            aGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCgg                                     <  4:178394/150‑1 (MQ=255)
                                                                                                             gTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCggg                                    <  1:1043273/150‑1 (MQ=255)
                                                                                                                  aaTTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCccg                                               <  1:1351986/134‑1 (MQ=255)
                                                                                                                  aaTTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGata                               >  2:1504085/1‑150 (MQ=255)
                                                                                                                    ttCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGTGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATAtt                             <  2:1371726/150‑1 (MQ=255)
                                                                                                                     tctcAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCg                                                                                              <  2:1082470/85‑1 (MQ=255)
                                                                                                                     tctcAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCg                                                                                              >  1:1082470/1‑85 (MQ=255)
                                                                                                                     tctcAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCa                                                               >  4:784665/1‑115 (MQ=255)
                                                                                                                     tctcAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCa                                                               <  3:784665/115‑1 (MQ=255)
                                                                                                                       tcAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGaa                                                                                                                               <  4:718196/50‑1 (MQ=255)
                                                                                                                       tcAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGaa                                                                                                                               >  3:718196/1‑50 (MQ=255)
                                                                                                                       tcAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGCATATcgcgcg                                                                  <  3:832299/110‑1 (MQ=255)
                                                                                                                       tcAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGCATATcgcgcg                                                                  >  4:832299/1‑110 (MQ=255)
                                                                                                                       tcAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGc                          <  4:231482/150‑1 (MQ=255)
                                                                                                                        cAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCccggc                                             >  2:1309915/1‑130 (MQ=255)
                                                                                                                        cAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCccggc                                             <  1:1309915/130‑1 (MQ=255)
                                                                                                                        cAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGct                         >  1:509913/1‑150 (MQ=255)
                                                                                                                         aaaaTCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCACAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGctc                        >  3:552295/1‑150 (MQ=255)
                                                                                                                          aaaTCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCcgcg                                                                                <  4:3550/94‑1 (MQ=255)
                                                                                                                          aaaTCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCcgcg                                                                                >  3:3550/1‑94 (MQ=255)
                                                                                                                          aaaTCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGctct                       >  2:345823/1‑150 (MQ=255)
                                                                                                                          aaaTCAGAAGAGTACTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGctct                       >  2:673947/1‑150 (MQ=255)
                                                                                                                            aTCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGGATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGc                     <  2:909160/150‑1 (MQ=255)
                                                                                                                                gaagaGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATgcg                                                                      >  3:311992/1‑97 (MQ=255)
                                                                                                                                gaagaGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATgcg                                                                      <  4:311992/97‑1 (MQ=255)
                                                                                                                                     gaaTTGCTAATGGAAAGACACGCTCCGAAACTTGCAGCATCGGTCCCAACGTCGTTGAAGCTTTCGGCGGTATCGCCCCCCCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGgatgat            <  3:584514/148‑1 (MQ=255)
                                                                                                                                      tATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCcgcgc                                                                               >  4:487971/1‑83 (MQ=255)
                                                                                                                                        ttGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGAtctt         >  2:331077/1‑150 (MQ=255)
                                                                                                                                            tAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCgg                                     <  1:486632/118‑1 (MQ=255)
                                                                                                                                            tAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCgg                                     >  2:486632/1‑118 (MQ=255)
                                                                                                                                            tAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTc                            >  2:1136514/1‑127 (MQ=255)
                                                                                                                                            tAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTc                            <  1:1136514/127‑1 (MQ=255)
                                                                                                                                            tAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGt     >  2:776338/1‑150 (MQ=255)
                                                                                                                                             aaTGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGtt    >  2:528296/1‑150 (MQ=255)
                                                                                                                                             aaTGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGtt    <  1:331077/150‑1 (MQ=255)
                                                                                                                                              aTGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGcc                                                                                     <  2:549651/69‑1 (MQ=255)
                                                                                                                                              aTGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTc   <  1:345823/150‑1 (MQ=255)
                                                                                                                                               tGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGAGATTCGCTCTGCCTGGATGATCTTCTGTTCg  >  1:902915/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
GTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCG  >  NC_000913_3_bme_pgi/4233616‑4233908

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: